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The map label for this gene is recN [H]

Identifier: 154686688

GI number: 154686688

Start: 2394403

End: 2396133

Strand: Reverse

Name: recN [H]

Synonym: RBAM_022570

Alternate gene names: 154686688

Gene position: 2396133-2394403 (Counterclockwise)

Preceding gene: 154686689

Following gene: 154686687

Centisome position: 61.15

GC content: 47.95

Gene sequence:

>1731_bases
TTGTTAGCTGAACTATCAATAAAAAACTTTGCCATTATAGAGGAACTGACGATTTCGTTTGAACGGGGGCTCACCGTCCT
GACGGGAGAAACAGGGGCCGGCAAGTCAATTATTATAGACGCCGTTTCCCTATTAGTCGGCGGCCGCGGCTCGTCTGAAT
TTGTCCGTTACGGTGAAACAAAGGCCGAGCTTGAAGGACTGTTTCTTTTGGACAGCGGTCATCCCGTTTTTGAAGTATGC
CGTGAGCAGGGAATAGACGCATCTGACGACATGATTGTCATGAGAAGGGACATTAATGCCGGAGGAAAAAGCGTCTGCCG
TGTCAACGGAAAATTAGTGACGATTGCGGCTTTAAGGGAAATCGGCAGACTTTTATTAGATATTCACGGACAGCATGATA
ACCAGCTGTTAATGGAGGACGATAAACACTTAGAGCTGTTGGACAGGTTTGCGGGCGCTGAGGCCGAATCCGCGCTTCAG
GCTTACCATGAAGGCTATGAGCGTTATATGAAGCTGCTCAAAAAAGCGAAGAAGCTGTCAGAAAGTGAGCAGGAAATGGC
GCATCGGTTAGACCTGATTCAATTTCAGCTTGAAGAAATCGAGTCGGCGAAGCTTGAATTGAATGAAGACGAGCTTCTTC
AGGAAGAGCGGAAGCAGATATCCAACTTTGAAAAAATTTATGAATCATTGCAAAACGCCTATAATGCGCTCCGCAGCGAG
CAGGGGGGGCTTGACTGGGTCGGAATGGCCTCTGCCCAGCTTGAAGATATTTCGGATATCAATGAACCGCTGCAAAAGCT
GTCAGAAAGTGTGTCAAGTTCCTATTATCTGCTGGAGGATGCAACCTTTCAGATGCGCAATCTGTTAGATGATCTGGAAT
ATGACCCGGAACGGCTGAATTTTATTGAAACCCGTTTAAATGAGATCAAACAGCTGAAGCGCAAATACGGAGCGACGGTA
GAAGACATTTTGGAATACGGCTCCAAAATAGAAGAGGAAATCGATCAAATCGAGAACCGTGACAGCCACCTTGAAGCGCT
GAAGAAGGAATTGGAGTCGGTCGGCAAAGATGTGGCCGTAGAAGCCGCCAATTTATCAAAAATCAGAAAAGCTTGGGCTA
AGAAACTGGCGGAAGCGATTCATCAGGAATTAAAAAGTCTGTATATGGGCAAATCCACGTTTGATACGGAATTTCTCGTG
AAAACCGATCCGTCAGCCAGTGAAGCACCGGTTGTTAACGGGCAGCCCGTGCAGCTTACCCAAAAGGGGATTGACCTTGT
GAAGTTTTTAATTTCAACAAACACCGGTGAGCCTTTAAAACCGCTGTCAAAGGTCGCTTCCGGAGGCGAGCTTTCAAGGG
TGATGCTGGCGATGAAGAGTATTTTTTCTTCCCAGCAGGATGTCACCTCCATCATCTTTGACGAAGTGGACACCGGTGTA
AGCGGGCGGGTTGCCCAAGCCATCGCGGAAAAAATCCATAAAGTATCGACCGGTTCACAGGTGCTCTGCATTACACACCT
GCCGCAGGTTGCCGCTATGGCGGATACGCACCTTCTGATCGCAAAAGAATTCAAAGACGGACGGACGACAACGCATGTCA
CTCCGCTTTCCAAACAGGACAAGGTAGCGGAAATCGGCCGCATGATTGCGGGCGTTGAAGTGACCGATCTGACAAAACGG
CATGCACAAGAGCTTTTAAAACAGGCCGGACAGGTAAAAACGACAGGATAA

Upstream 100 bases:

>100_bases
ATCTTAATCATCTGCAGAACGCCTGAAGATACGGAGGGCGTTCAAAGCCGTCTTTTGGAGCTGCTGTAGCGTAATTATCA
GATAAAAGGTGGAATGCTGT

Downstream 100 bases:

>100_bases
GCTGCTGATACAGGCAGCTTATTTTTTTGTTCCATTTCTCTAACAGTGTATCCAATGTTTTTCTTCGTATGCCGGTTATA
AATCAAGCGCAGGAAGGCAA

Product: hypothetical protein

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MLAELSIKNFAIIEELTISFERGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEFVRYGETKAELEGLFLLDSGHPVFEVC
REQGIDASDDMIVMRRDINAGGKSVCRVNGKLVTIAALREIGRLLLDIHGQHDNQLLMEDDKHLELLDRFAGAEAESALQ
AYHEGYERYMKLLKKAKKLSESEQEMAHRLDLIQFQLEEIESAKLELNEDELLQEERKQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLQKLSESVSSSYYLLEDATFQMRNLLDDLEYDPERLNFIETRLNEIKQLKRKYGATV
EDILEYGSKIEEEIDQIENRDSHLEALKKELESVGKDVAVEAANLSKIRKAWAKKLAEAIHQELKSLYMGKSTFDTEFLV
KTDPSASEAPVVNGQPVQLTQKGIDLVKFLISTNTGEPLKPLSKVASGGELSRVMLAMKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSTGSQVLCITHLPQVAAMADTHLLIAKEFKDGRTTTHVTPLSKQDKVAEIGRMIAGVEVTDLTKR
HAQELLKQAGQVKTTG

Sequences:

>Translated_576_residues
MLAELSIKNFAIIEELTISFERGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEFVRYGETKAELEGLFLLDSGHPVFEVC
REQGIDASDDMIVMRRDINAGGKSVCRVNGKLVTIAALREIGRLLLDIHGQHDNQLLMEDDKHLELLDRFAGAEAESALQ
AYHEGYERYMKLLKKAKKLSESEQEMAHRLDLIQFQLEEIESAKLELNEDELLQEERKQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLQKLSESVSSSYYLLEDATFQMRNLLDDLEYDPERLNFIETRLNEIKQLKRKYGATV
EDILEYGSKIEEEIDQIENRDSHLEALKKELESVGKDVAVEAANLSKIRKAWAKKLAEAIHQELKSLYMGKSTFDTEFLV
KTDPSASEAPVVNGQPVQLTQKGIDLVKFLISTNTGEPLKPLSKVASGGELSRVMLAMKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSTGSQVLCITHLPQVAAMADTHLLIAKEFKDGRTTTHVTPLSKQDKVAEIGRMIAGVEVTDLTKR
HAQELLKQAGQVKTTG
>Mature_576_residues
MLAELSIKNFAIIEELTISFERGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEFVRYGETKAELEGLFLLDSGHPVFEVC
REQGIDASDDMIVMRRDINAGGKSVCRVNGKLVTIAALREIGRLLLDIHGQHDNQLLMEDDKHLELLDRFAGAEAESALQ
AYHEGYERYMKLLKKAKKLSESEQEMAHRLDLIQFQLEEIESAKLELNEDELLQEERKQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLQKLSESVSSSYYLLEDATFQMRNLLDDLEYDPERLNFIETRLNEIKQLKRKYGATV
EDILEYGSKIEEEIDQIENRDSHLEALKKELESVGKDVAVEAANLSKIRKAWAKKLAEAIHQELKSLYMGKSTFDTEFLV
KTDPSASEAPVVNGQPVQLTQKGIDLVKFLISTNTGEPLKPLSKVASGGELSRVMLAMKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSTGSQVLCITHLPQVAAMADTHLLIAKEFKDGRTTTHVTPLSKQDKVAEIGRMIAGVEVTDLTKR
HAQELLKQAGQVKTTG

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=575, Percent_Identity=33.9130434782609, Blast_Score=300, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 64053; Mature: 64053

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAELSIKNFAIIEELTISFERGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEFVRYGET
CCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAELEGLFLLDSGHPVFEVCREQGIDASDDMIVMRRDINAGGKSVCRVNGKLVTIAALRE
HHHHCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEECCCEEHHHHHHH
IGRLLLDIHGQHDNQLLMEDDKHLELLDRFAGAEAESALQAYHEGYERYMKLLKKAKKLS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ESEQEMAHRLDLIQFQLEEIESAKLELNEDELLQEERKQISNFEKIYESLQNAYNALRSE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QGGLDWVGMASAQLEDISDINEPLQKLSESVSSSYYLLEDATFQMRNLLDDLEYDPERLN
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCHHHHH
FIETRLNEIKQLKRKYGATVEDILEYGSKIEEEIDQIENRDSHLEALKKELESVGKDVAV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHH
EAANLSKIRKAWAKKLAEAIHQELKSLYMGKSTFDTEFLVKTDPSASEAPVVNGQPVQLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
QKGIDLVKFLISTNTGEPLKPLSKVASGGELSRVMLAMKSIFSSQQDVTSIIFDEVDTGV
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
SGRVAQAIAEKIHKVSTGSQVLCITHLPQVAAMADTHLLIAKEFKDGRTTTHVTPLSKQD
CHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHEEEHHCCCCCCEEEECCCCCCH
KVAEIGRMIAGVEVTDLTKRHAQELLKQAGQVKTTG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLAELSIKNFAIIEELTISFERGLTVLTGETGAGKSIIIDAVSLLVGGRGSSEFVRYGET
CCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAELEGLFLLDSGHPVFEVCREQGIDASDDMIVMRRDINAGGKSVCRVNGKLVTIAALRE
HHHHCEEEEEECCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEECCCEEHHHHHHH
IGRLLLDIHGQHDNQLLMEDDKHLELLDRFAGAEAESALQAYHEGYERYMKLLKKAKKLS
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ESEQEMAHRLDLIQFQLEEIESAKLELNEDELLQEERKQISNFEKIYESLQNAYNALRSE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QGGLDWVGMASAQLEDISDINEPLQKLSESVSSSYYLLEDATFQMRNLLDDLEYDPERLN
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCHHHHH
FIETRLNEIKQLKRKYGATVEDILEYGSKIEEEIDQIENRDSHLEALKKELESVGKDVAV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHH
EAANLSKIRKAWAKKLAEAIHQELKSLYMGKSTFDTEFLVKTDPSASEAPVVNGQPVQLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
QKGIDLVKFLISTNTGEPLKPLSKVASGGELSRVMLAMKSIFSSQQDVTSIIFDEVDTGV
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
SGRVAQAIAEKIHKVSTGSQVLCITHLPQVAAMADTHLLIAKEFKDGRTTTHVTPLSKQD
CHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHEEEHHCCCCCCEEEECCCCCCH
KVAEIGRMIAGVEVTDLTKRHAQELLKQAGQVKTTG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]