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The map label for this gene is spo0A [H]

Identifier: 154686686

GI number: 154686686

Start: 2391878

End: 2392678

Strand: Reverse

Name: spo0A [H]

Synonym: RBAM_022550

Alternate gene names: 154686686

Gene position: 2392678-2391878 (Counterclockwise)

Preceding gene: 154686687

Following gene: 154686680

Centisome position: 61.06

GC content: 47.19

Gene sequence:

>801_bases
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGTATTATCCGCAGCCAGCCGGAACCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACTGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTAGAGCATAAAGCTTCGTA
A

Upstream 100 bases:

>100_bases
AATATGGATTTTTTAAAAGGGGAAGTAGTGGTTTTGTCGAATGTAACATGTAGCAGTCAAATGGGTTGCGTCCTGTTAAT
ACATTGGGGGAGGAAGAAAC

Downstream 100 bases:

>100_bases
CGCAAGCTTTTGGCTAGATGTCTTTTTCGCGCCTTCTATTCCGTGCATGAACGCGGAAGAGAAGGCGTTTTGAAATTTTG
CTGGCGAAAACAATGGAAAT

Product: Spo0A

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: Stage 0 sporulation protein C; Stage 0 sporulation protein G [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Sequences:

>Translated_266_residues
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS
>Mature_266_residues
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=111, Percent_Identity=40.5405405405405, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788550, Length=82, Percent_Identity=39.0243902439024, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=140, Percent_Identity=33.5714285714286, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 29630; Mature: 29630

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHCCCCCEEEEEEHHH
HLDGLAVLERLRESELEKQPNVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSIIHEIGVPAHIKGYLYLREAIS
HHCCCHHHHHHCCCCHHHHHHHCCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEE
MTKAKPTNSEFIAMVADKLRLEHKAS
EECCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMP
CCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHCCCCCEEEEEEHHH
HLDGLAVLERLRESELEKQPNVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIR
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
QVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSIIHEIGVPAHIKGYLYLREAIS
HHCCCHHHHHHCCCCHHHHHHHCCCCCHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEE
MTKAKPTNSEFIAMVADKLRLEHKAS
EECCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3921963; 3018427; 3157992; 8969508; 9384377; 9044256; 3151993; 2121995; 12176382 [H]