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The map label for this gene is mmgD [H]

Identifier: 154686673

GI number: 154686673

Start: 2380073

End: 2381170

Strand: Reverse

Name: mmgD [H]

Synonym: RBAM_022420

Alternate gene names: 154686673

Gene position: 2381170-2380073 (Counterclockwise)

Preceding gene: 154686674

Following gene: 154686672

Centisome position: 60.77

GC content: 50.09

Gene sequence:

>1098_bases
ATGGAATCTTATTCTCCCGGTTTAGATGGCATCATTGCGGTGGAAACGGGGATTTCTTTCCTGGATACACAGCAGGGCAG
TATTTTAATCAAAGGCCACGATCTGATTGAGCTTTCTAAAACGGCCGGCTATATGGACATTGTTCATCTCTTATTGGAAG
ACAGACTTCCTGATGTGAAAGAGAAGGCAGCGCTTGAAGAAGACGTTCGGCGCGGCGGACACCTTCCCGAGGAAATCTTG
CACATATGCCGTCTGCTGCCCGAACATACTCACCCGATGGACGGATTGAGGACGGGTTTGTCTGCGCTTGGAGGCTTTGA
CAAGGACATCGATGACCGTTCATCTTCCGTAAATCAGCACCGCGTACGCGGACTGATAGGCAAAATGCCGAATCTGACGG
TCAACAGCTATCGAGTCATCAACGGGCTGGAGCCTTTGGCTCCGGTCGGAACCTTATCATACAGCGCAAACTTTTTATAT
ATGATTACCGGAAAAACGCCGTCATCTTTGGAAGAAGAAATCTTCGACAGGTCGCTTCTTTTATACAGTGAGCATGAATT
GCCGAACTCAACGTTTACGGCCCGTGTCATCGCTTCGACGCAATCGGATATTTACGGCGCGCTGACCGGAGCCGTCGCCT
CGCTTAAGGGAAATCTTCACGGCGGAGCGAACGAAGCGGTTATGTACATGCTTGAGGAAGCGAAGGATACAGACGGGTTT
ACACAATTATTAACTCAAAAACTCGCAAACAAAGAAAAAATCATGGGCTTCGGCCACCGTGTCTACATGAAAAAAATGGA
CCCGAGGGCGCTCATGATGAAAGAAGCGTTGAAACAGCTGTCTGATCTTTCAGGTGATTTCCGTCTTTATCATATGTGTG
AAGCCGGCGAAACCGTCATGCGGGAGAAAAAAGGCCTTTATCCGAATTTAGATTACTACGCAGCGCCGGTCTATTACATG
CTCGGCATCCCGATACCGCTGTACACGCCTATATTCTTCAGCGCACGGACGGTCGGACTGTGCGCTCACGTCATTGAACA
GCACAGCCGAAACCGGCTTTTCCGCCCGCGTGTCCGCTATACCGGAGCGCGCCTGTAA

Upstream 100 bases:

>100_bases
ATGCGAAAGTTACGCAAATCTATGAAGGAACCAATGAGATTCAAAGGCTGATTATTTCTAAGTATCTTCTTCAATAAAAA
GTGCGCAAAGGAGCGAAACC

Downstream 100 bases:

>100_bases
TCAGATGAGAAAGGAAGATGAAAACATGACAGAACCCCGCCGAATGGACGCTGTAATCGAAGAGATTTCTGACTACGTGC
TCCATCAGGAGATTGACAGT

Product: citrate synthase 3

Products: NA

Alternate protein names: Citrate synthase 3; Citrate synthase III; Methylcitrate synthase [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MESYSPGLDGIIAVETGISFLDTQQGSILIKGHDLIELSKTAGYMDIVHLLLEDRLPDVKEKAALEEDVRRGGHLPEEIL
HICRLLPEHTHPMDGLRTGLSALGGFDKDIDDRSSSVNQHRVRGLIGKMPNLTVNSYRVINGLEPLAPVGTLSYSANFLY
MITGKTPSSLEEEIFDRSLLLYSEHELPNSTFTARVIASTQSDIYGALTGAVASLKGNLHGGANEAVMYMLEEAKDTDGF
TQLLTQKLANKEKIMGFGHRVYMKKMDPRALMMKEALKQLSDLSGDFRLYHMCEAGETVMREKKGLYPNLDYYAAPVYYM
LGIPIPLYTPIFFSARTVGLCAHVIEQHSRNRLFRPRVRYTGARL

Sequences:

>Translated_365_residues
MESYSPGLDGIIAVETGISFLDTQQGSILIKGHDLIELSKTAGYMDIVHLLLEDRLPDVKEKAALEEDVRRGGHLPEEIL
HICRLLPEHTHPMDGLRTGLSALGGFDKDIDDRSSSVNQHRVRGLIGKMPNLTVNSYRVINGLEPLAPVGTLSYSANFLY
MITGKTPSSLEEEIFDRSLLLYSEHELPNSTFTARVIASTQSDIYGALTGAVASLKGNLHGGANEAVMYMLEEAKDTDGF
TQLLTQKLANKEKIMGFGHRVYMKKMDPRALMMKEALKQLSDLSGDFRLYHMCEAGETVMREKKGLYPNLDYYAAPVYYM
LGIPIPLYTPIFFSARTVGLCAHVIEQHSRNRLFRPRVRYTGARL
>Mature_365_residues
MESYSPGLDGIIAVETGISFLDTQQGSILIKGHDLIELSKTAGYMDIVHLLLEDRLPDVKEKAALEEDVRRGGHLPEEIL
HICRLLPEHTHPMDGLRTGLSALGGFDKDIDDRSSSVNQHRVRGLIGKMPNLTVNSYRVINGLEPLAPVGTLSYSANFLY
MITGKTPSSLEEEIFDRSLLLYSEHELPNSTFTARVIASTQSDIYGALTGAVASLKGNLHGGANEAVMYMLEEAKDTDGF
TQLLTQKLANKEKIMGFGHRVYMKKMDPRALMMKEALKQLSDLSGDFRLYHMCEAGETVMREKKGLYPNLDYYAAPVYYM
LGIPIPLYTPIFFSARTVGLCAHVIEQHSRNRLFRPRVRYTGARL

Specific function: Catalyzes the synthesis of citrate from acetyl-CoA and oxaloacetate. The genomic context suggests that this protein probably functions as a 2-methylcitrate synthase, i.e. catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=371, Percent_Identity=25.0673854447439, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786527, Length=359, Percent_Identity=34.8189415041783, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1786939, Length=379, Percent_Identity=31.6622691292876, Blast_Score=178, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=373, Percent_Identity=25.7372654155496, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=376, Percent_Identity=25.531914893617, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319850, Length=327, Percent_Identity=25.3822629969419, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640126, Length=375, Percent_Identity=25.6, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640124, Length=375, Percent_Identity=25.6, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5; =2.3.3.1 [H]

Molecular weight: Translated: 40742; Mature: 40742

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESYSPGLDGIIAVETGISFLDTQQGSILIKGHDLIELSKTAGYMDIVHLLLEDRLPDVK
CCCCCCCCCCEEEEECCCHHEECCCCEEEEECCCEEEEHHHCCHHHHHHHHHHHCCCCHH
EKAALEEDVRRGGHLPEEILHICRLLPEHTHPMDGLRTGLSALGGFDKDIDDRSSSVNQH
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RVRGLIGKMPNLTVNSYRVINGLEPLAPVGTLSYSANFLYMITGKTPSSLEEEIFDRSLL
HHHHHHHCCCCCEECCEEEECCCCCCCCCCCEEECCCEEEEEECCCCCHHHHHHHCCHHE
LYSEHELPNSTFTARVIASTQSDIYGALTGAVASLKGNLHGGANEAVMYMLEEAKDTDGF
EEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHH
TQLLTQKLANKEKIMGFGHRVYMKKMDPRALMMKEALKQLSDLSGDFRLYHMCEAGETVM
HHHHHHHHCCHHHHHHCCHHEEEHHCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
REKKGLYPNLDYYAAPVYYMLGIPIPLYTPIFFSARTVGLCAHVIEQHSRNRLFRPRVRY
HHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TGARL
CCCCC
>Mature Secondary Structure
MESYSPGLDGIIAVETGISFLDTQQGSILIKGHDLIELSKTAGYMDIVHLLLEDRLPDVK
CCCCCCCCCCEEEEECCCHHEECCCCEEEEECCCEEEEHHHCCHHHHHHHHHHHCCCCHH
EKAALEEDVRRGGHLPEEILHICRLLPEHTHPMDGLRTGLSALGGFDKDIDDRSSSVNQH
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RVRGLIGKMPNLTVNSYRVINGLEPLAPVGTLSYSANFLYMITGKTPSSLEEEIFDRSLL
HHHHHHHCCCCCEECCEEEECCCCCCCCCCCEEECCCEEEEEECCCCCHHHHHHHCCHHE
LYSEHELPNSTFTARVIASTQSDIYGALTGAVASLKGNLHGGANEAVMYMLEEAKDTDGF
EEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHH
TQLLTQKLANKEKIMGFGHRVYMKKMDPRALMMKEALKQLSDLSGDFRLYHMCEAGETVM
HHHHHHHHCCHHHHHHCCHHEEEHHCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
REKKGLYPNLDYYAAPVYYMLGIPIPLYTPIFFSARTVGLCAHVIEQHSRNRLFRPRVRY
HHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TGARL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759838; 8969508; 9384377 [H]