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The map label for this gene is prpD [H]

Identifier: 154686672

GI number: 154686672

Start: 2378620

End: 2380047

Strand: Reverse

Name: prpD [H]

Synonym: RBAM_022410

Alternate gene names: 154686672

Gene position: 2380047-2378620 (Counterclockwise)

Preceding gene: 154686673

Following gene: 154686671

Centisome position: 60.74

GC content: 51.19

Gene sequence:

>1428_bases
ATGACAGAACCCCGCCGAATGGACGCTGTAATCGAAGAGATTTCTGACTACGTGCTCCATCAGGAGATTGACAGTTCAGA
AGCTTTTACTACGGCAGGACATGTGCTTCTTGATACGATAGGCTGCGGAATTTTGGCGCTCAGCTATCCCGAATGTACAA
AGCTGCTCGGCCCGATTGTCCCGGGAACCGCCGTGCCCAACGGCAGCAGAGTCCCGGGCACGCCTTTTGTTTTAGACCCG
GTCACGGCTGCTTTTAATATCGGCTGTATCAACAGGTGGCTCGATTATAATGATACGTGGCTTGCTGCGGAATGGGGTCA
TCCGTCTGACAATCTCGGAGGCATATTGGCTTGTGCGGATTATATATCCAGAGTGCGGATTTCAGAAGGGAAAGAACCGC
TTACCGTGCGCGATGTGCTTTGCGCAATGATCAAAGCGCACGAAATTCAAGGAATTCTCGCTTTGGAAAACAGCTTAAAC
AGAGTGGGGCTTGACCATGTGCTGTTTGTGAAAGTGGCGACTGCGGCTGTTTGCGCGCAAATGCTGGGCGGCTCAAAAGA
GGAAATCAATAACGCCGTATCTCATGCCTGGATTGATAATTCAAGCCTCAGAACGTACCGTCATGCGCCGAACACCGGAT
CACGCAAATCATGGGCGGCCGGCGACGCGACGAGCAGAGGGGTATATTTGGCCCTTATGGCGCACAAAGGTGAAATGGGG
TACCCGACGGCTCTGACCGCATCTGGGTGGGGATTTCAAGACGTATTGTTTAACCGGCGGGAAATAAAGCTTGCCCGCCC
GCTTGGGTCTTATGTGATGGAGAATGTTCTGTTTAAAGTATCCTACCCGGCTGAATTCCATGCCCAGACAGCGGCGGAAT
GCGCGGTCCGGCTTCATCCGGAGGTGAAAGACCGCATCGAAGACATTGACAGGATCACGATTATGACGCATGAGTCGGCA
ATCCGGATTATCGATAAAAAAGGGCCGCTTCACAATCCGGCTGACCGGGATCACTGCCTCCAGTACATTACGGCAATCGG
GCTGCTTTTCGGAGATATCACGGCCGATCATTATGAAGAGGAAACAGCGTCTGACCCGCGAATTGATACATTGCGGAGCA
AAATGGAGGTGACGGAACACAAAGCGTACTCAAAAGATTACTTAGACCCGAATAAGCGATCCATATCAAATGCCGTACAG
GTGCATTTTACAGACGGCACGGCTACGGGACGGATTGAATGTGAATACCCGCTCGGGCACCGGTTCCGCAGAAAAGAAGC
CGTTCCGAAGATTCTTGCCAAGTTTTCAGCAAACATCAGCACTCATTTTTCATTGAAGCAGCAGCGAAAAATCGAAGAGG
CCTGCGAACATAGCGGCCGAATGCAGAAGCTCAGCGTCAGTGAATTTATGGACTTATTTCTCGTATAA

Upstream 100 bases:

>100_bases
GCTCACGTCATTGAACAGCACAGCCGAAACCGGCTTTTCCGCCCGCGTGTCCGCTATACCGGAGCGCGCCTGTAATCAGA
TGAGAAAGGAAGATGAAAAC

Downstream 100 bases:

>100_bases
AATAGGAGGGTGACAAACATGACGTGGATCGTCAGCAAGCAGTCGTCTCAAGAGGAGCTTGCAGAGCGGTTCCGTACGCT
GATGACAGCGCCGGATCTAC

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTEPRRMDAVIEEISDYVLHQEIDSSEAFTTAGHVLLDTIGCGILALSYPECTKLLGPIVPGTAVPNGSRVPGTPFVLDP
VTAAFNIGCINRWLDYNDTWLAAEWGHPSDNLGGILACADYISRVRISEGKEPLTVRDVLCAMIKAHEIQGILALENSLN
RVGLDHVLFVKVATAAVCAQMLGGSKEEINNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVYLALMAHKGEMG
YPTALTASGWGFQDVLFNRREIKLARPLGSYVMENVLFKVSYPAEFHAQTAAECAVRLHPEVKDRIEDIDRITIMTHESA
IRIIDKKGPLHNPADRDHCLQYITAIGLLFGDITADHYEEETASDPRIDTLRSKMEVTEHKAYSKDYLDPNKRSISNAVQ
VHFTDGTATGRIECEYPLGHRFRRKEAVPKILAKFSANISTHFSLKQQRKIEEACEHSGRMQKLSVSEFMDLFLV

Sequences:

>Translated_475_residues
MTEPRRMDAVIEEISDYVLHQEIDSSEAFTTAGHVLLDTIGCGILALSYPECTKLLGPIVPGTAVPNGSRVPGTPFVLDP
VTAAFNIGCINRWLDYNDTWLAAEWGHPSDNLGGILACADYISRVRISEGKEPLTVRDVLCAMIKAHEIQGILALENSLN
RVGLDHVLFVKVATAAVCAQMLGGSKEEINNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVYLALMAHKGEMG
YPTALTASGWGFQDVLFNRREIKLARPLGSYVMENVLFKVSYPAEFHAQTAAECAVRLHPEVKDRIEDIDRITIMTHESA
IRIIDKKGPLHNPADRDHCLQYITAIGLLFGDITADHYEEETASDPRIDTLRSKMEVTEHKAYSKDYLDPNKRSISNAVQ
VHFTDGTATGRIECEYPLGHRFRRKEAVPKILAKFSANISTHFSLKQQRKIEEACEHSGRMQKLSVSEFMDLFLV
>Mature_474_residues
TEPRRMDAVIEEISDYVLHQEIDSSEAFTTAGHVLLDTIGCGILALSYPECTKLLGPIVPGTAVPNGSRVPGTPFVLDPV
TAAFNIGCINRWLDYNDTWLAAEWGHPSDNLGGILACADYISRVRISEGKEPLTVRDVLCAMIKAHEIQGILALENSLNR
VGLDHVLFVKVATAAVCAQMLGGSKEEINNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVYLALMAHKGEMGY
PTALTASGWGFQDVLFNRREIKLARPLGSYVMENVLFKVSYPAEFHAQTAAECAVRLHPEVKDRIEDIDRITIMTHESAI
RIIDKKGPLHNPADRDHCLQYITAIGLLFGDITADHYEEETASDPRIDTLRSKMEVTEHKAYSKDYLDPNKRSISNAVQV
HFTDGTATGRIECEYPLGHRFRRKEAVPKILAKFSANISTHFSLKQQRKIEEACEHSGRMQKLSVSEFMDLFLV

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=472, Percent_Identity=61.0169491525424, Blast_Score=612, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6325258, Length=477, Percent_Identity=51.9916142557652, Blast_Score=496, Evalue=1e-141,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 52718; Mature: 52586

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPRRMDAVIEEISDYVLHQEIDSSEAFTTAGHVLLDTIGCGILALSYPECTKLLGPIV
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHEEECCHHHHHHHCCCC
PGTAVPNGSRVPGTPFVLDPVTAAFNIGCINRWLDYNDTWLAAEWGHPSDNLGGILACAD
CCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHH
YISRVRISEGKEPLTVRDVLCAMIKAHEIQGILALENSLNRVGLDHVLFVKVATAAVCAQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHH
MLGGSKEEINNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVYLALMAHKGEMG
HHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
YPTALTASGWGFQDVLFNRREIKLARPLGSYVMENVLFKVSYPAEFHAQTAAECAVRLHP
CCCEEECCCCCHHHHHHCCHHEEEHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCC
EVKDRIEDIDRITIMTHESAIRIIDKKGPLHNPADRDHCLQYITAIGLLFGDITADHYEE
HHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ETASDPRIDTLRSKMEVTEHKAYSKDYLDPNKRSISNAVQVHFTDGTATGRIECEYPLGH
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEECCCCCCEEEECCCCCH
RFRRKEAVPKILAKFSANISTHFSLKQQRKIEEACEHSGRMQKLSVSEFMDLFLV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCC
>Mature Secondary Structure 
TEPRRMDAVIEEISDYVLHQEIDSSEAFTTAGHVLLDTIGCGILALSYPECTKLLGPIV
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHEEECCHHHHHHHCCCC
PGTAVPNGSRVPGTPFVLDPVTAAFNIGCINRWLDYNDTWLAAEWGHPSDNLGGILACAD
CCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHH
YISRVRISEGKEPLTVRDVLCAMIKAHEIQGILALENSLNRVGLDHVLFVKVATAAVCAQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHH
MLGGSKEEINNAVSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVYLALMAHKGEMG
HHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
YPTALTASGWGFQDVLFNRREIKLARPLGSYVMENVLFKVSYPAEFHAQTAAECAVRLHP
CCCEEECCCCCHHHHHHCCHHEEEHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCC
EVKDRIEDIDRITIMTHESAIRIIDKKGPLHNPADRDHCLQYITAIGLLFGDITADHYEE
HHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ETASDPRIDTLRSKMEVTEHKAYSKDYLDPNKRSISNAVQVHFTDGTATGRIECEYPLGH
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEEECCCCCCEEEECCCCCH
RFRRKEAVPKILAKFSANISTHFSLKQQRKIEEACEHSGRMQKLSVSEFMDLFLV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]