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The map label for this gene is bkdAB [H]

Identifier: 154686663

GI number: 154686663

Start: 2368523

End: 2369506

Strand: Reverse

Name: bkdAB [H]

Synonym: RBAM_022320

Alternate gene names: 154686663

Gene position: 2369506-2368523 (Counterclockwise)

Preceding gene: 154686664

Following gene: 154686662

Centisome position: 60.47

GC content: 49.19

Gene sequence:

>984_bases
ATGTCTGTAATGTCTTATATTGACAGTATTAATGCAGCAATGAAAGAAGAAATGGAAAGAGATCCGCGCGTTTTTGTTCT
CGGGGAAGACGTCGGGAGAAAAGGCGGCGTGTTTAAAGCCACGGCGGGTCTCTATGAACAGTTCGGAGAAGAGCGGGTGA
TGGATACGCCGCTTGCTGAATCAGCGATTGCCGGTGTAGGGATCGGCGCCGCGATGTACGGCATGCGCCCGATTGCCGAG
ATGCAGTTTGCCGATTTCATTCTGCCTGCGGTCAACCAAATCATTTCAGAAGCGGCAAAAATACGCTACCGTTCAAACAA
TGATTGGAGCTGTCCGATGGTCATCAGAGCGCCTTACGGCGGCGGCGTGCACGGCGCGTTGTATCATTCCCAATCTGTGG
AGGCCATTTTTGCCAACCAGCCGGGCCTTAAAATTGTCATGCCTTCAACTCCTTATGACGCAAAAGGGCTGTTGAAAGCT
GCAGTCCGTGACGAAGATCCGGTGCTGTTTTTCGAACATAAGAGAGCGTACCGTCTGATTAAAGGCGAAGTCCCTGCGGA
TGATTACGTCCTTCCGATCGGAAAGGCGGATGTAAAACGCGAAGGCAGCGACATTACTGTTATTACGTACGGATTATGCG
TCCATTTTGCTCTGCAGGCTGCCGAGCGCCTTGAAAAAGACGGAATTTCCGCACACGTATTAGATTTGCGTACGGTTTAT
CCGTTAGATAAAGAAGCCATCATTGAAGCCGCGTCTAAAACAGGAAAAGTATTGCTGATCACAGAAGATACGAAAGAAGG
CGGTATTATGAGCGAGGTCGCCGCTATTATTTCTGAAAACTGCTTATTTGATTTAGACGCGCCGATCAAGCGGCTTGCAG
GTCCTGACGTTCCGGCAATGCCTTATGCGCCGACAATGGAAAAATACTTCATGATGAATCCTGACAAAGCAGAAGCCGCG
ATGAGAGAATTAGCGGAGTTTTAA

Upstream 100 bases:

>100_bases
GGCTATCGTAAACGAAGCGACAGACGAAGCGGAGAAAGCGCCATACGCATCTCCTGAATCAACGCTTGATCACGTTTATG
CGAAGTAGGGAGGAATACTT

Downstream 100 bases:

>100_bases
AGACGTAAGGAGGTTTTTGACATGGCGATTGAACAAATGGCTATGCCTCAGCTCGGAGAGAGCGTGACGGAAGGGACGAT
CAGCAAGTGGCTGGTATCCC

Product: BkdAB

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSVMSYIDSINAAMKEEMERDPRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAESAIAGVGIGAAMYGMRPIAE
MQFADFILPAVNQIISEAAKIRYRSNNDWSCPMVIRAPYGGGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKA
AVRDEDPVLFFEHKRAYRLIKGEVPADDYVLPIGKADVKREGSDITVITYGLCVHFALQAAERLEKDGISAHVLDLRTVY
PLDKEAIIEAASKTGKVLLITEDTKEGGIMSEVAAIISENCLFDLDAPIKRLAGPDVPAMPYAPTMEKYFMMNPDKAEAA
MRELAEF

Sequences:

>Translated_327_residues
MSVMSYIDSINAAMKEEMERDPRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAESAIAGVGIGAAMYGMRPIAE
MQFADFILPAVNQIISEAAKIRYRSNNDWSCPMVIRAPYGGGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKA
AVRDEDPVLFFEHKRAYRLIKGEVPADDYVLPIGKADVKREGSDITVITYGLCVHFALQAAERLEKDGISAHVLDLRTVY
PLDKEAIIEAASKTGKVLLITEDTKEGGIMSEVAAIISENCLFDLDAPIKRLAGPDVPAMPYAPTMEKYFMMNPDKAEAA
MRELAEF
>Mature_326_residues
SVMSYIDSINAAMKEEMERDPRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAESAIAGVGIGAAMYGMRPIAEM
QFADFILPAVNQIISEAAKIRYRSNNDWSCPMVIRAPYGGGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAA
VRDEDPVLFFEHKRAYRLIKGEVPADDYVLPIGKADVKREGSDITVITYGLCVHFALQAAERLEKDGISAHVLDLRTVYP
LDKEAIIEAASKTGKVLLITEDTKEGGIMSEVAAIISENCLFDLDAPIKRLAGPDVPAMPYAPTMEKYFMMNPDKAEAAM
RELAEF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=326, Percent_Identity=42.3312883435583, Blast_Score=282, Evalue=3e-76,
Organism=Homo sapiens, GI34101272, Length=326, Percent_Identity=42.3312883435583, Blast_Score=282, Evalue=3e-76,
Organism=Homo sapiens, GI156564403, Length=304, Percent_Identity=40.1315789473684, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI291084858, Length=304, Percent_Identity=39.1447368421053, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI133778974, Length=173, Percent_Identity=30.0578034682081, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=310, Percent_Identity=43.8709677419355, Blast_Score=261, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17506935, Length=316, Percent_Identity=43.6708860759494, Blast_Score=251, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6319698, Length=312, Percent_Identity=40.7051282051282, Blast_Score=246, Evalue=3e-66,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=42.0245398773006, Blast_Score=262, Evalue=2e-70,
Organism=Drosophila melanogaster, GI160714832, Length=326, Percent_Identity=42.0245398773006, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI21358145, Length=326, Percent_Identity=40.1840490797546, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24650940, Length=326, Percent_Identity=40.1840490797546, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=41.1111111111111, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=41.1111111111111, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 35792; Mature: 35660

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVMSYIDSINAAMKEEMERDPRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAE
CCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHCCCHHCCCCHHH
SAIAGVGIGAAMYGMRPIAEMQFADFILPAVNQIISEAAKIRYRSNNDWSCPMVIRAPYG
HHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEECCCC
GGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAAVRDEDPVLFFEHKRAYRLI
CCCCHHEECCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHH
KGEVPADDYVLPIGKADVKREGSDITVITYGLCVHFALQAAERLEKDGISAHVLDLRTVY
CCCCCCCCEEEECCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEHEEC
PLDKEAIIEAASKTGKVLLITEDTKEGGIMSEVAAIISENCLFDLDAPIKRLAGPDVPAM
CCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCHHHHHHCCCCCCCC
PYAPTMEKYFMMNPDKAEAAMRELAEF
CCCCCHHHHEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SVMSYIDSINAAMKEEMERDPRVFVLGEDVGRKGGVFKATAGLYEQFGEERVMDTPLAE
CHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHCCCHHCCCCHHH
SAIAGVGIGAAMYGMRPIAEMQFADFILPAVNQIISEAAKIRYRSNNDWSCPMVIRAPYG
HHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEECCCC
GGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAAVRDEDPVLFFEHKRAYRLI
CCCCHHEECCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHH
KGEVPADDYVLPIGKADVKREGSDITVITYGLCVHFALQAAERLEKDGISAHVLDLRTVY
CCCCCCCCEEEECCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEHEEC
PLDKEAIIEAASKTGKVLLITEDTKEGGIMSEVAAIISENCLFDLDAPIKRLAGPDVPAM
CCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCHHHHHHCCCCCCCC
PYAPTMEKYFMMNPDKAEAAMRELAEF
CCCCCHHHHEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]