Click here to switch to the map view.

The map label for this gene is bkdAA [H]

Identifier: 154686664

GI number: 154686664

Start: 2369519

End: 2370511

Strand: Reverse

Name: bkdAA [H]

Synonym: RBAM_022330

Alternate gene names: 154686664

Gene position: 2370511-2369519 (Counterclockwise)

Preceding gene: 154686665

Following gene: 154686663

Centisome position: 60.49

GC content: 49.75

Gene sequence:

>993_bases
ATGACTACAAACCGCCATCAAGCATTAGGGCTCTCTGACGAAGAAGCCGTTGAAATGTATAGAACCATGCTGTTAGCCAG
AAAAATCGACGAAAGAATGTGGCTCTTAAACCGCTCGGGCAAAATTCCGTTCGTTGTTTCATGTCAAGGACAGGAAGCGG
CGCAAGTAGGCGCGGCCTATGCCTTAAACCGTGATACCGACTATGTGCTTCCGTACTACAGAGATATAGGGGTTGTGCTT
GCATTCGGTATGACTGCAAAGGATTTGATGATGTCGGGTTTTGCGAAAGCAGCTGACCCGAACTCAGGCGGAAGGCAGAT
GCCGGGCCACTTCGGGCAAAAGAAAAACAGAATCGTAACGGGCTCATCCCCTGTTACGACGCAGGTGCCTCATGCAGTCG
GAATCGCGCTTGCGGGACGCATGGATAAAAAGGATATCGTCTCTTTCGTTACCTTTGGAGAAGGATCTTCCAACCAGGGG
GATTTTCACGAAGGGGCAAACTTTGCCGCCGTGTATAAACTGCCGGTCATTTTCATGTGTGAAAACAATAAGTATGCTAT
TTCTGTCCCGTATGACAGACAGGTGGCGTGCGAGAAAATATCCGACCGGGCGGCAGGCTACGGCATGCCGGGAGTCACTG
TTGACGGAAACGACCTGCTGGAAGTTTACCGGGCCGTAAAAGAAGCGCGCGAACGGGCGTCTAAAGGCGAAGGCCCTACA
TTAATAGAAACGGTTTCCTACCGTTTGACACCGCATTCCAGTGATGACGACGACAGCAGCTACAGAGAACGCGAAGAAGT
AGAAGAAGCAAAGAAACAAGATCCGCTTCTTTTATACAAAGCATACTTGAAAGAAGCCGGGCTTCTAGGTGAAGAAGAAG
AAAAGACCATGCTGGATGACATCATGGCTATCGTAAACGAAGCGACAGACGAAGCGGAGAAAGCGCCATACGCATCTCCT
GAATCAACGCTTGATCACGTTTATGCGAAGTAG

Upstream 100 bases:

>100_bases
ATACATCCGCATCCGTCGCTGTCAGAAGCGATTGGAGAAGCTGCGTTAGCCGCAGACGGAAACGCGATTCATTTTTAAAA
GGAAAGAAGGAGGGGTTTGA

Downstream 100 bases:

>100_bases
GGAGGAATACTTATGTCTGTAATGTCTTATATTGACAGTATTAATGCAGCAATGAAAGAAGAAATGGAAAGAGATCCGCG
CGTTTTTGTTCTCGGGGAAG

Product: BkdAA

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTTNRHQALGLSDEEAVEMYRTMLLARKIDERMWLLNRSGKIPFVVSCQGQEAAQVGAAYALNRDTDYVLPYYRDIGVVL
AFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGRMDKKDIVSFVTFGEGSSNQG
DFHEGANFAAVYKLPVIFMCENNKYAISVPYDRQVACEKISDRAAGYGMPGVTVDGNDLLEVYRAVKEARERASKGEGPT
LIETVSYRLTPHSSDDDDSSYREREEVEEAKKQDPLLLYKAYLKEAGLLGEEEEKTMLDDIMAIVNEATDEAEKAPYASP
ESTLDHVYAK

Sequences:

>Translated_330_residues
MTTNRHQALGLSDEEAVEMYRTMLLARKIDERMWLLNRSGKIPFVVSCQGQEAAQVGAAYALNRDTDYVLPYYRDIGVVL
AFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGRMDKKDIVSFVTFGEGSSNQG
DFHEGANFAAVYKLPVIFMCENNKYAISVPYDRQVACEKISDRAAGYGMPGVTVDGNDLLEVYRAVKEARERASKGEGPT
LIETVSYRLTPHSSDDDDSSYREREEVEEAKKQDPLLLYKAYLKEAGLLGEEEEKTMLDDIMAIVNEATDEAEKAPYASP
ESTLDHVYAK
>Mature_329_residues
TTNRHQALGLSDEEAVEMYRTMLLARKIDERMWLLNRSGKIPFVVSCQGQEAAQVGAAYALNRDTDYVLPYYRDIGVVLA
FGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGRMDKKDIVSFVTFGEGSSNQGD
FHEGANFAAVYKLPVIFMCENNKYAISVPYDRQVACEKISDRAAGYGMPGVTVDGNDLLEVYRAVKEARERASKGEGPTL
IETVSYRLTPHSSDDDDSSYREREEVEEAKKQDPLLLYKAYLKEAGLLGEEEEKTMLDDIMAIVNEATDEAEKAPYASPE
STLDHVYAK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=319, Percent_Identity=39.4984326018809, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI258645172, Length=319, Percent_Identity=39.4984326018809, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI291084742, Length=325, Percent_Identity=32.9230769230769, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4505685, Length=325, Percent_Identity=32.9230769230769, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4885543, Length=327, Percent_Identity=32.7217125382263, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI291084744, Length=332, Percent_Identity=32.2289156626506, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI291084757, Length=176, Percent_Identity=36.9318181818182, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI86563355, Length=327, Percent_Identity=38.2262996941896, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI86563357, Length=327, Percent_Identity=38.2262996941896, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI32564172, Length=323, Percent_Identity=31.8885448916409, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17536047, Length=323, Percent_Identity=31.8885448916409, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=32.2884012539185, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21355903, Length=323, Percent_Identity=34.0557275541796, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24639748, Length=310, Percent_Identity=33.8709677419355, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24639740, Length=310, Percent_Identity=31.2903225806452, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24639744, Length=306, Percent_Identity=31.3725490196078, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI28571106, Length=306, Percent_Identity=31.3725490196078, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24639746, Length=266, Percent_Identity=33.0827067669173, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 36393; Mature: 36262

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNRHQALGLSDEEAVEMYRTMLLARKIDERMWLLNRSGKIPFVVSCQGQEAAQVGAAY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHE
ALNRDTDYVLPYYRDIGVVLAFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVT
EECCCCCEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
GSSPVTTQVPHAVGIALAGRMDKKDIVSFVTFGEGSSNQGDFHEGANFAAVYKLPVIFMC
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCHHCCCCEEEEEECCEEEEE
ENNKYAISVPYDRQVACEKISDRAAGYGMPGVTVDGNDLLEVYRAVKEARERASKGEGPT
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCE
LIETVSYRLTPHSSDDDDSSYREREEVEEAKKQDPLLLYKAYLKEAGLLGEEEEKTMLDD
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
IMAIVNEATDEAEKAPYASPESTLDHVYAK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TTNRHQALGLSDEEAVEMYRTMLLARKIDERMWLLNRSGKIPFVVSCQGQEAAQVGAAY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHE
ALNRDTDYVLPYYRDIGVVLAFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVT
EECCCCCEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
GSSPVTTQVPHAVGIALAGRMDKKDIVSFVTFGEGSSNQGDFHEGANFAAVYKLPVIFMC
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCHHCCCCEEEEEECCEEEEE
ENNKYAISVPYDRQVACEKISDRAAGYGMPGVTVDGNDLLEVYRAVKEARERASKGEGPT
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCE
LIETVSYRLTPHSSDDDDSSYREREEVEEAKKQDPLLLYKAYLKEAGLLGEEEEKTMLDD
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
IMAIVNEATDEAEKAPYASPESTLDHVYAK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]