Click here to switch to the map view.

The map label for this gene is bkdB [H]

Identifier: 154686662

GI number: 154686662

Start: 2367239

End: 2368501

Strand: Reverse

Name: bkdB [H]

Synonym: RBAM_022310

Alternate gene names: 154686662

Gene position: 2368501-2367239 (Counterclockwise)

Preceding gene: 154686663

Following gene: 154686660

Centisome position: 60.44

GC content: 48.38

Gene sequence:

>1263_bases
ATGGCGATTGAACAAATGGCTATGCCTCAGCTCGGAGAGAGCGTGACGGAAGGGACGATCAGCAAGTGGCTGGTATCCCC
CGGTGATCAAGTGAATAAATATGACCCCATCGCGGAGGTCATGACAGATAAAGTGAACGCGGAGGTGCCGTCTTCTTTTA
CCGGTACGATTAGAGAACTTGTAGGAGAAGAAGGCCAGACTCTTGCAGTCGGCGAGATCATCTGCAAAATTGAAACAGAA
GAAACGGAAACCGAAGAGGCGCCGAAGCGGGAAGAAGAGCAGAATAGCCCTTCAGACACTGATGCAAACCGTCAAGGTAA
AGATCAGTCAAATAAAGCGCGTTATTCGCCGGCCGTGCTCAGATTAGCGGGAGAACACGGCATTCAGCTTGAAGAAGTTG
AAGGAACGGGTGCAGGCGGAAGAATTACGCGAAAAGACATTCAAAAGATCATTGATTCGGGAATGAAGCAGAAGTCGGAA
GCACCGGCGCCGGCTGCCGTTCAAACAACGCAACAAGCGCCGAAACAACAGCAAGAACAAGCACCGGCACCAGCTCTGTC
AGCCGGTGATATTGAAATTCCGGTTACAGGCGTCAGAAAAGCCATCGCCGCCAATATGCATAAAAGCAAAACAGAAATTC
CGCATGCCTGGACGATGATGGAAGTGGACGTCACGAATATGACGGCGTACAGAAACAGCATTAAAGACTCATTTAAACAA
AAAGAAGGCTTCAATTTAACGTTTTTCGCTTTCTTTGTAAAAGCGGCGGCGCAGGCTCTCAAAGAGTTCCCGCAGATGAA
CAGCATGTGGGCGGGAGACAAAATCGTCCAAAAGAAAGACATCAACATCTCCATTGCCGTTGCCACGGAAGATTCGCTGT
TTGTTCCGGTTATTAAGCATGCTGATGAAAAAACGATTAAAGGCATAGCCAGAGAAATAACGGACCTTGCGAAAAAAGTG
AGAAACGGAAAACTTACCGCGGAAGATATGCAGGGCGGCACATTTACCGTCAATAACACGGGATCTTTCGGCTCAGTTCA
ATCTATGGGCATTATCAACCATCCGCAGGCGGCGATTCTTCAGGTGGAGTCTATCGTCAAACGTCCGGTTGTCATGAATA
ACGGGATGATCGCCGTTCGTGATATGGTTAATCTTTGCCTGTCTTTAGATCACAGAGTGCTCGACGGGCTCGTGTGCGGC
CGATTCCTCGGACGCGTCAAAGAGATCTTAGAAAACATTGATGAACATACTTCCGTCTATTAA

Upstream 100 bases:

>100_bases
TGCGCCGACAATGGAAAAATACTTCATGATGAATCCTGACAAAGCAGAAGCCGCGATGAGAGAATTAGCGGAGTTTTAAA
GACGTAAGGAGGTTTTTGAC

Downstream 100 bases:

>100_bases
AAAAAACTCCTTTTGCCCGGGCGGCAAAAGGAGTTTTTTTATTTTCATTGTGAATCCTCGGGAACAATCATAGTGAGATG
ATGTTTTTTCACTTCGATCG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MAIEQMAMPQLGESVTEGTISKWLVSPGDQVNKYDPIAEVMTDKVNAEVPSSFTGTIRELVGEEGQTLAVGEIICKIETE
ETETEEAPKREEEQNSPSDTDANRQGKDQSNKARYSPAVLRLAGEHGIQLEEVEGTGAGGRITRKDIQKIIDSGMKQKSE
APAPAAVQTTQQAPKQQQEQAPAPALSAGDIEIPVTGVRKAIAANMHKSKTEIPHAWTMMEVDVTNMTAYRNSIKDSFKQ
KEGFNLTFFAFFVKAAAQALKEFPQMNSMWAGDKIVQKKDINISIAVATEDSLFVPVIKHADEKTIKGIAREITDLAKKV
RNGKLTAEDMQGGTFTVNNTGSFGSVQSMGIINHPQAAILQVESIVKRPVVMNNGMIAVRDMVNLCLSLDHRVLDGLVCG
RFLGRVKEILENIDEHTSVY

Sequences:

>Translated_420_residues
MAIEQMAMPQLGESVTEGTISKWLVSPGDQVNKYDPIAEVMTDKVNAEVPSSFTGTIRELVGEEGQTLAVGEIICKIETE
ETETEEAPKREEEQNSPSDTDANRQGKDQSNKARYSPAVLRLAGEHGIQLEEVEGTGAGGRITRKDIQKIIDSGMKQKSE
APAPAAVQTTQQAPKQQQEQAPAPALSAGDIEIPVTGVRKAIAANMHKSKTEIPHAWTMMEVDVTNMTAYRNSIKDSFKQ
KEGFNLTFFAFFVKAAAQALKEFPQMNSMWAGDKIVQKKDINISIAVATEDSLFVPVIKHADEKTIKGIAREITDLAKKV
RNGKLTAEDMQGGTFTVNNTGSFGSVQSMGIINHPQAAILQVESIVKRPVVMNNGMIAVRDMVNLCLSLDHRVLDGLVCG
RFLGRVKEILENIDEHTSVY
>Mature_419_residues
AIEQMAMPQLGESVTEGTISKWLVSPGDQVNKYDPIAEVMTDKVNAEVPSSFTGTIRELVGEEGQTLAVGEIICKIETEE
TETEEAPKREEEQNSPSDTDANRQGKDQSNKARYSPAVLRLAGEHGIQLEEVEGTGAGGRITRKDIQKIIDSGMKQKSEA
PAPAAVQTTQQAPKQQQEQAPAPALSAGDIEIPVTGVRKAIAANMHKSKTEIPHAWTMMEVDVTNMTAYRNSIKDSFKQK
EGFNLTFFAFFVKAAAQALKEFPQMNSMWAGDKIVQKKDINISIAVATEDSLFVPVIKHADEKTIKGIAREITDLAKKVR
NGKLTAEDMQGGTFTVNNTGSFGSVQSMGIINHPQAAILQVESIVKRPVVMNNGMIAVRDMVNLCLSLDHRVLDGLVCGR
FLGRVKEILENIDEHTSVY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=418, Percent_Identity=31.5789473684211, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI203098753, Length=449, Percent_Identity=29.8440979955457, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI203098816, Length=449, Percent_Identity=29.8440979955457, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=29.4252873563218, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI19923748, Length=217, Percent_Identity=38.2488479262673, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=39.4904458598726, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1786946, Length=415, Percent_Identity=34.2168674698795, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=31.002331002331, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=419, Percent_Identity=29.3556085918854, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI25146366, Length=407, Percent_Identity=31.9410319410319, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=29.6629213483146, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=28.8961038961039, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=408, Percent_Identity=31.6176470588235, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6324258, Length=443, Percent_Identity=27.313769751693, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI18859875, Length=422, Percent_Identity=28.6729857819905, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=37.2093023255814, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24582497, Length=223, Percent_Identity=33.1838565022422, Blast_Score=144, Evalue=9e-35,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 45895; Mature: 45764

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEQMAMPQLGESVTEGTISKWLVSPGDQVNKYDPIAEVMTDKVNAEVPSSFTGTIREL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
VGEEGQTLAVGEIICKIETEETETEEAPKREEEQNSPSDTDANRQGKDQSNKARYSPAVL
HCCCCCEEEEEEEEEEEECCCCCHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLAGEHGIQLEEVEGTGAGGRITRKDIQKIIDSGMKQKSEAPAPAAVQTTQQAPKQQQEQ
EEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
APAPALSAGDIEIPVTGVRKAIAANMHKSKTEIPHAWTMMEVDVTNMTAYRNSIKDSFKQ
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHH
KEGFNLTFFAFFVKAAAQALKEFPQMNSMWAGDKIVQKKDINISIAVATEDSLFVPVIKH
CCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHEEEECCEEEEEEECCCCEEEHHHHC
ADEKTIKGIAREITDLAKKVRNGKLTAEDMQGGTFTVNNTGSFGSVQSMGIINHPQAAIL
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCCCCCCCHHCCCCCCCCHHHHH
QVESIVKRPVVMNNGMIAVRDMVNLCLSLDHRVLDGLVCGRFLGRVKEILENIDEHTSVY
HHHHHHHCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIEQMAMPQLGESVTEGTISKWLVSPGDQVNKYDPIAEVMTDKVNAEVPSSFTGTIREL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
VGEEGQTLAVGEIICKIETEETETEEAPKREEEQNSPSDTDANRQGKDQSNKARYSPAVL
HCCCCCEEEEEEEEEEEECCCCCHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLAGEHGIQLEEVEGTGAGGRITRKDIQKIIDSGMKQKSEAPAPAAVQTTQQAPKQQQEQ
EEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
APAPALSAGDIEIPVTGVRKAIAANMHKSKTEIPHAWTMMEVDVTNMTAYRNSIKDSFKQ
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHH
KEGFNLTFFAFFVKAAAQALKEFPQMNSMWAGDKIVQKKDINISIAVATEDSLFVPVIKH
CCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHEEEECCEEEEEEECCCCEEEHHHHC
ADEKTIKGIAREITDLAKKVRNGKLTAEDMQGGTFTVNNTGSFGSVQSMGIINHPQAAIL
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCCCCCCCHHCCCCCCCCHHHHH
QVESIVKRPVVMNNGMIAVRDMVNLCLSLDHRVLDGLVCGRFLGRVKEILENIDEHTSVY
HHHHHHHCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]