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The map label for this gene is aspA [H]

Identifier: 154686601

GI number: 154686601

Start: 2257733

End: 2259160

Strand: Reverse

Name: aspA [H]

Synonym: RBAM_021700

Alternate gene names: 154686601

Gene position: 2259160-2257733 (Counterclockwise)

Preceding gene: 154686602

Following gene: 154686600

Centisome position: 57.65

GC content: 48.11

Gene sequence:

>1428_bases
ATGACAAACGCGCAAAAAGAATATCGTCAGGAAAAAGATTTCCTCGGAGAAAAACAAATTGAAGCTGATGTGTATTACGG
AATACAAACGCTGCGCGCGAAAGAAAACTTTCCGATTACCGGCTACCGCATCCACGAAGAAATGATTAATGCGCTCGCCA
TCGTCAAAAAAGCGGCGGCGCTTGCCAACATGGATGTAAAACGGCTGTATGAAGGAATCGGAAATGCGATTGTACAGGCC
GCAGATGAAATTCTTGAGGGAAAATGGCATGACCAATTTATCGTCGATCCGATCCAAGGGGGAGCCGGAACGTCCATGAA
CATGAATGCCAACGAAGTAATCGGCAACAGGGCTCTTGAAATCATGGGGCATAAAAAAGGTGAATACATTCACCTCAGCC
CGAACACACACGTCAACATGTCACAATCGACGAACGACGTGTTCCCGACGGCAATCCATATTTCAACCTTGAAACTTTTG
GATAAGCTCCTTGATACGATGGCGCATATGCTCAGTGTCTTTAAAGATAAAGCGAAACAATTTGATTCTGTCATTAAAAT
GGGGCGCACACACCTTCAGGACGCTGTGCCGATCCGTCTCGGACAGGAATTTGAAGCATACAGCCGCGTGCTTGAGCGTG
ACATAAAGCGAATTAAACAATCCCGCCAGCATCTGTATGAAGTAAACATGGGGGCAACGGCCGTAGGTACGGGACTTAAC
GCCGATCCGCGCTACATAGAACAGGTTGTGAAGCACCTGGCTGACATCAGCGGACTGCCGCTTGTCGGCGCCGGCCATTT
GGTTGACGCCACACAAAACACTGATGCGTATACGGAAGTTTCAGCCGCGTTGAAAGTATGCATGATGAACATGTCTAAAA
TCGCCAACGATCTGCGCTTAATGGCTTCAGGTCCGCGCGCGGGACTTGCGGAAATTTCACTGCCCGCCCGTCAGCCGGGA
TCATCCATTATGCCTGGGAAAGTCAACCCTGTAATGGCAGAGCTGATCAACCAAATCGCCTTCCAGGTCATCGGGAATGA
CAATACGATCTGCCTCGCTTCAGAAGCGGGCCAGCTGGAGCTTAACGTAATGGAGCCGGTACTCGTATTTAACCTGCTTC
AATCCATCAGCATCATGAATAACGGGTTCCGTTCATTTACCGATCATTGTCTGGCGGGAATTGAAGCGAACGAAAAACGT
CTGAAGCAATACGTGGAAAAAAGCGTCGGCGTCATTACGGCGGTCAATCCGCACCTCGGGTATGAAGCGGCTGCGCGAAT
TGCACGTGAAGCAATCATGACAGGGCAATCTGTAAGAGATCTTTGCCTTCAAAATGATGTACTGACAGAAGAAGAATTGG
ATATCATTTTGAATCCGTATGAAATGACAAAACCGGGGATTGCCGGAAAAGATCTATTAGAAAAATAA

Upstream 100 bases:

>100_bases
CACAACTTGGCTGAAGTCAAAAACATTATGGAGACACCGATCGCAGACGACGTTGTACTGTAAAAACATGACTACCACAA
AGAGAAAGTAGGGTTTTAGC

Downstream 100 bases:

>100_bases
ATTTTAAAGCAACAACAGCCGCCTTTTCACGTTTTTTAGTGAATAGGCGGTTGCCATACAAAGGGATGAGGCAGTATAGT
ATAATTTGTTATATCATTTG

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTNAQKEYRQEKDFLGEKQIEADVYYGIQTLRAKENFPITGYRIHEEMINALAIVKKAAALANMDVKRLYEGIGNAIVQA
ADEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALEIMGHKKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLL
DKLLDTMAHMLSVFKDKAKQFDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLN
ADPRYIEQVVKHLADISGLPLVGAGHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRAGLAEISLPARQPG
SSIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRSFTDHCLAGIEANEKR
LKQYVEKSVGVITAVNPHLGYEAAARIAREAIMTGQSVRDLCLQNDVLTEEELDIILNPYEMTKPGIAGKDLLEK

Sequences:

>Translated_475_residues
MTNAQKEYRQEKDFLGEKQIEADVYYGIQTLRAKENFPITGYRIHEEMINALAIVKKAAALANMDVKRLYEGIGNAIVQA
ADEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALEIMGHKKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLL
DKLLDTMAHMLSVFKDKAKQFDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLN
ADPRYIEQVVKHLADISGLPLVGAGHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRAGLAEISLPARQPG
SSIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRSFTDHCLAGIEANEKR
LKQYVEKSVGVITAVNPHLGYEAAARIAREAIMTGQSVRDLCLQNDVLTEEELDIILNPYEMTKPGIAGKDLLEK
>Mature_474_residues
TNAQKEYRQEKDFLGEKQIEADVYYGIQTLRAKENFPITGYRIHEEMINALAIVKKAAALANMDVKRLYEGIGNAIVQAA
DEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALEIMGHKKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLD
KLLDTMAHMLSVFKDKAKQFDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLNA
DPRYIEQVVKHLADISGLPLVGAGHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRAGLAEISLPARQPGS
SIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRSFTDHCLAGIEANEKRL
KQYVEKSVGVITAVNPHLGYEAAARIAREAIMTGQSVRDLCLQNDVLTEEELDIILNPYEMTKPGIAGKDLLEK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=465, Percent_Identity=42.3655913978495, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI68303547, Length=212, Percent_Identity=26.4150943396226, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI31541964, Length=238, Percent_Identity=26.4705882352941, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI68303542, Length=238, Percent_Identity=26.4705882352941, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=48.3870967741936, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=42.548596112311, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1790398, Length=209, Percent_Identity=27.7511961722488, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=43.695652173913, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=42.5076452599388, Blast_Score=283, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=42.5162689804772, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640177, Length=466, Percent_Identity=41.8454935622318, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640179, Length=466, Percent_Identity=41.8454935622318, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=42.1166306695464, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24662684, Length=471, Percent_Identity=41.4012738853503, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=37.2844827586207, Blast_Score=325, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52397; Mature: 52266

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAQKEYRQEKDFLGEKQIEADVYYGIQTLRAKENFPITGYRIHEEMINALAIVKKAAA
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LANMDVKRLYEGIGNAIVQAADEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALE
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
IMGHKKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLDKLLDTMAHMLSVFKDKAKQ
HHCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPRYIEQVVKHLADISGLPLVGAGHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHCCCCCCEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRAGLAEISLPARQPGSSIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLE
HCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRSFTDHCLAGIEANEKRLKQYVEKSVGVITAVNPHLG
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARIAREAIMTGQSVRDLCLQNDVLTEEELDIILNPYEMTKPGIAGKDLLEK
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
TNAQKEYRQEKDFLGEKQIEADVYYGIQTLRAKENFPITGYRIHEEMINALAIVKKAAA
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LANMDVKRLYEGIGNAIVQAADEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALE
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
IMGHKKGEYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLDKLLDTMAHMLSVFKDKAKQ
HHCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPRYIEQVVKHLADISGLPLVGAGHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHCCCCCCEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRAGLAEISLPARQPGSSIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLE
HCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRSFTDHCLAGIEANEKRLKQYVEKSVGVITAVNPHLG
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARIAREAIMTGQSVRDLCLQNDVLTEEELDIILNPYEMTKPGIAGKDLLEK
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]