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The map label for this gene is ansA [H]

Identifier: 154686602

GI number: 154686602

Start: 2259198

End: 2260187

Strand: Reverse

Name: ansA [H]

Synonym: RBAM_021710

Alternate gene names: 154686602

Gene position: 2260187-2259198 (Counterclockwise)

Preceding gene: 154686604

Following gene: 154686601

Centisome position: 57.68

GC content: 45.76

Gene sequence:

>990_bases
ATGAAAAAATTATTGCTGTTAACAACCGGAGGAACAATCGCCTCAGTCGAAGGAGAAAACGGGCTTGCACCCGGGGTGAA
AGCAGACGAATTGTTAACCTACGTATCAAAACTTGATAACGATTACACGATGGAAACCCATTCGTTAATGAACATCGACA
GCACAAATATGCAGCCTGAATACTGGGCCGAAATCGCAAATGCTGTCAAAGAAAAATATGACGCATATGACGGGTTCGTC
ATTACGCACGGAACAGACACAATGGCCTATACATCAGCGGCACTGTCTTATATGCTTCAGCATGCTGATAAACCGATCGT
CATTACGGGTTCGCAAATCCCGATTACATTTCAAAAAACCGATGCCAAAAAAAATATCACGGACGCGATCCGCTTCGCAT
GTGAAGGGGTGGGCGGCGTATACGTCGTATTTGACGGCAGAGTCATTCAAGGCACACGCGCCATTAAATTAAGAACGAAA
AGCTATGATGCATTTGAGAGCATTAACTATCCGTATATCGCGTTTATTACCGAAAACGGAATTGAATACAACAAACAAGT
GCACATGCCAAAAGACGATGCTTTTACGGTCAATACTTCACTTTGCACGGACGTGTGCCTGCTCAAGCTCCACCCGGGGC
TGAAACCGGAAATGTTTGACGCATTAAAGGGCATGTACAAAGGAATCGTCATTGAAAGCTACGGCAGCGGCGGCGTGCCG
TTTGAAGGAAGAGACATTCTCGCCAAAGTCAATGAAATGATTGAAAGCGGCATTGTCGTCGTCATTACGACACAATGTCT
TGAAGAAGGAGAGGACATGAGCGTCTATGAAGTCGGCCGAAGAGTCAATCAAGATCTCATTATCCGCTCCAGAAATATGA
ACACGGAAGCCATCGTGCCGAAATTAATGTGGGCGCTCGGCCAGTCTCACAACTTGGCTGAAGTCAAAAACATTATGGAG
ACACCGATCGCAGACGACGTTGTACTGTAA

Upstream 100 bases:

>100_bases
TCACTTGATTAATTTCATTCGTCTGTCTATAATTTGATTAAAAGTATGCGAAAAGCGAATGCTTAACACATCTTGAGAAA
TGGATGAAGAGGTGCAGGGT

Downstream 100 bases:

>100_bases
AAACATGACTACCACAAAGAGAAAGTAGGGTTTTAGCATGACAAACGCGCAAAAAGAATATCGTCAGGAAAAAGATTTCC
TCGGAGAAAAACAAATTGAA

Product: AnsA

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKLLLLTTGGTIASVEGENGLAPGVKADELLTYVSKLDNDYTMETHSLMNIDSTNMQPEYWAEIANAVKEKYDAYDGFV
ITHGTDTMAYTSAALSYMLQHADKPIVITGSQIPITFQKTDAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTK
SYDAFESINYPYIAFITENGIEYNKQVHMPKDDAFTVNTSLCTDVCLLKLHPGLKPEMFDALKGMYKGIVIESYGSGGVP
FEGRDILAKVNEMIESGIVVVITTQCLEEGEDMSVYEVGRRVNQDLIIRSRNMNTEAIVPKLMWALGQSHNLAEVKNIME
TPIADDVVL

Sequences:

>Translated_329_residues
MKKLLLLTTGGTIASVEGENGLAPGVKADELLTYVSKLDNDYTMETHSLMNIDSTNMQPEYWAEIANAVKEKYDAYDGFV
ITHGTDTMAYTSAALSYMLQHADKPIVITGSQIPITFQKTDAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTK
SYDAFESINYPYIAFITENGIEYNKQVHMPKDDAFTVNTSLCTDVCLLKLHPGLKPEMFDALKGMYKGIVIESYGSGGVP
FEGRDILAKVNEMIESGIVVVITTQCLEEGEDMSVYEVGRRVNQDLIIRSRNMNTEAIVPKLMWALGQSHNLAEVKNIME
TPIADDVVL
>Mature_329_residues
MKKLLLLTTGGTIASVEGENGLAPGVKADELLTYVSKLDNDYTMETHSLMNIDSTNMQPEYWAEIANAVKEKYDAYDGFV
ITHGTDTMAYTSAALSYMLQHADKPIVITGSQIPITFQKTDAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTK
SYDAFESINYPYIAFITENGIEYNKQVHMPKDDAFTVNTSLCTDVCLLKLHPGLKPEMFDALKGMYKGIVIESYGSGGVP
FEGRDILAKVNEMIESGIVVVITTQCLEEGEDMSVYEVGRRVNQDLIIRSRNMNTEAIVPKLMWALGQSHNLAEVKNIME
TPIADDVVL

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=261, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1788065, Length=328, Percent_Identity=31.4024390243902, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789327, Length=163, Percent_Identity=31.2883435582822, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=260, Percent_Identity=34.6153846153846, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17558280, Length=260, Percent_Identity=34.6153846153846, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17558278, Length=260, Percent_Identity=34.6153846153846, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI193207374, Length=260, Percent_Identity=34.6153846153846, Blast_Score=164, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6320527, Length=263, Percent_Identity=27.7566539923954, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323189, Length=279, Percent_Identity=26.1648745519713, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323187, Length=279, Percent_Identity=26.1648745519713, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323186, Length=279, Percent_Identity=26.1648745519713, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323184, Length=279, Percent_Identity=26.1648745519713, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645530, Length=338, Percent_Identity=31.9526627218935, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24639609, Length=274, Percent_Identity=33.9416058394161, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36380; Mature: 36380

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLLTTGGTIASVEGENGLAPGVKADELLTYVSKLDNDYTMETHSLMNIDSTNMQPE
CCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHEEECCCCCCCHH
YWAEIANAVKEKYDAYDGFVITHGTDTMAYTSAALSYMLQHADKPIVITGSQIPITFQKT
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECC
DAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTKSYDAFESINYPYIAFITENG
CCCCHHHHHHHHHHCCCCCEEEEECCEEEECCEEEEEEECCCHHHHHCCCCEEEEEECCC
IEYNKQVHMPKDDAFTVNTSLCTDVCLLKLHPGLKPEMFDALKGMYKGIVIESYGSGGVP
CCCCCEECCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
FEGRDILAKVNEMIESGIVVVITTQCLEEGEDMSVYEVGRRVNQDLIIRSRNMNTEAIVP
CCCHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHH
KLMWALGQSHNLAEVKNIMETPIADDVVL
HHHHHHCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKLLLLTTGGTIASVEGENGLAPGVKADELLTYVSKLDNDYTMETHSLMNIDSTNMQPE
CCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHEEECCCCCCCHH
YWAEIANAVKEKYDAYDGFVITHGTDTMAYTSAALSYMLQHADKPIVITGSQIPITFQKT
HHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCEEEEEEECC
DAKKNITDAIRFACEGVGGVYVVFDGRVIQGTRAIKLRTKSYDAFESINYPYIAFITENG
CCCCHHHHHHHHHHCCCCCEEEEECCEEEECCEEEEEEECCCHHHHHCCCCEEEEEECCC
IEYNKQVHMPKDDAFTVNTSLCTDVCLLKLHPGLKPEMFDALKGMYKGIVIESYGSGGVP
CCCCCEECCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
FEGRDILAKVNEMIESGIVVVITTQCLEEGEDMSVYEVGRRVNQDLIIRSRNMNTEAIVP
CCCHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHH
KLMWALGQSHNLAEVKNIMETPIADDVVL
HHHHHHCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]