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The map label for this gene is sleB [H]

Identifier: 154686539

GI number: 154686539

Start: 2204534

End: 2205403

Strand: Reverse

Name: sleB [H]

Synonym: RBAM_021080

Alternate gene names: 154686539

Gene position: 2205403-2204534 (Counterclockwise)

Preceding gene: 154686540

Following gene: 154686538

Centisome position: 56.28

GC content: 46.9

Gene sequence:

>870_bases
ATGAAGTGCAAAGGAGCCTTTATGGCATGTCTCATTCTATTTTCTTTCGCCGCTGCTTTCTTCGATCATCATGAAACGGT
TCATGCGTTTTCAAATCAAGTGATTCAAAGAGGCGCGACGGGTGATGATGTTATAGAATTGCAGGCCCGTCTTCAATATA
ACGGATTTTATAACGGGAAAATCGATGGTGTTTACGGCTGGACCACATACTGGGCCGTGAGAAATTTTCAGGAGAAATTC
GGCATGAAGCCGGTTGACGGCCTCGTCGGCGCGAAAACCAAACAGGTGCTGATTTCTAAATCAAAATATTACCGTGAATA
TGTAATGGAGCAGATCCAAAAAGGGAATACGTTCACTCATTATGGCAACGTCCCATTGAAAAACCAAACAAAACCGAGAG
CCGGAACGCAGAAGCCGGCGGGAACGAATAAGCCTGCTCAAAAGCCGCAAAACAAAACAGGCGGTCAGTCAAATCAAAAG
CAAAATACGACAGCGGCCAATATGCCTGGCGGTTTCTCAAACAACGACATCAATCTTCTGGCACAGGCTGTGTACGGGGA
AGCGCGCGGTGAGCCGTTTGAAGGGCAGGTTGCCATCGCCGCGGTTATTTTAAACCGTATTAAAAGCCCGTTATTTCCGA
ATACGGTGGCCGGCGTCATTTTCGAACCGCTCGCGTTTACCGCCGTTGCCGACGGCCAAATATATATGCAGCCGAATGAA
AGAGCAAAAGAAGCGGTTCTTGATGCGATCAACGGCTGGGATCCTTCTGAAAACGCCATTTATTATTTCAATCCTGATAC
GGCGACAAGCAAATGGATTTGGGGACGTCCGCAGATTAAAAGAATCGGAAAGCACATTTTCTGTGAGTAA

Upstream 100 bases:

>100_bases
TTATGCCTTGACAGGGGATAAGAAGGAAACGTGCATAAAAATAACCTCGTGACAGACAATACGGGTACACTTTAAATTTT
GCAGTAGGAGGCTTGAAGAG

Downstream 100 bases:

>100_bases
AAGTACGCAAAATGAGGTGTTAACATGATTAGAGGAATCATTATCGCTGTTCTCGGTGTCGCGATCGCCGCAACCGGATA
CTGGGGATATAAAGAGCATC

Product: hypothetical protein

Products: NA

Alternate protein names: SCLE [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKCKGAFMACLILFSFAAAFFDHHETVHAFSNQVIQRGATGDDVIELQARLQYNGFYNGKIDGVYGWTTYWAVRNFQEKF
GMKPVDGLVGAKTKQVLISKSKYYREYVMEQIQKGNTFTHYGNVPLKNQTKPRAGTQKPAGTNKPAQKPQNKTGGQSNQK
QNTTAANMPGGFSNNDINLLAQAVYGEARGEPFEGQVAIAAVILNRIKSPLFPNTVAGVIFEPLAFTAVADGQIYMQPNE
RAKEAVLDAINGWDPSENAIYYFNPDTATSKWIWGRPQIKRIGKHIFCE

Sequences:

>Translated_289_residues
MKCKGAFMACLILFSFAAAFFDHHETVHAFSNQVIQRGATGDDVIELQARLQYNGFYNGKIDGVYGWTTYWAVRNFQEKF
GMKPVDGLVGAKTKQVLISKSKYYREYVMEQIQKGNTFTHYGNVPLKNQTKPRAGTQKPAGTNKPAQKPQNKTGGQSNQK
QNTTAANMPGGFSNNDINLLAQAVYGEARGEPFEGQVAIAAVILNRIKSPLFPNTVAGVIFEPLAFTAVADGQIYMQPNE
RAKEAVLDAINGWDPSENAIYYFNPDTATSKWIWGRPQIKRIGKHIFCE
>Mature_289_residues
MKCKGAFMACLILFSFAAAFFDHHETVHAFSNQVIQRGATGDDVIELQARLQYNGFYNGKIDGVYGWTTYWAVRNFQEKF
GMKPVDGLVGAKTKQVLISKSKYYREYVMEQIQKGNTFTHYGNVPLKNQTKPRAGTQKPAGTNKPAQKPQNKTGGQSNQK
QNTTAANMPGGFSNNDINLLAQAVYGEARGEPFEGQVAIAAVILNRIKSPLFPNTVAGVIFEPLAFTAVADGQIYMQPNE
RAKEAVLDAINGWDPSENAIYYFNPDTATSKWIWGRPQIKRIGKHIFCE

Specific function: Could be a lytic transglycosylase. Required for spore cortex hydrolysis during germination. Interacts strongly but noncovalently with spore components [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 32130; Mature: 32130

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCKGAFMACLILFSFAAAFFDHHETVHAFSNQVIQRGATGDDVIELQARLQYNGFYNGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEHHEECCEECCE
IDGVYGWTTYWAVRNFQEKFGMKPVDGLVGAKTKQVLISKSKYYREYVMEQIQKGNTFTH
ECCEECHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YGNVPLKNQTKPRAGTQKPAGTNKPAQKPQNKTGGQSNQKQNTTAANMPGGFSNNDINLL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHH
AQAVYGEARGEPFEGQVAIAAVILNRIKSPLFPNTVAGVIFEPLAFTAVADGQIYMQPNE
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCEEEECCCH
RAKEAVLDAINGWDPSENAIYYFNPDTATSKWIWGRPQIKRIGKHIFCE
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKCKGAFMACLILFSFAAAFFDHHETVHAFSNQVIQRGATGDDVIELQARLQYNGFYNGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEHHEECCEECCE
IDGVYGWTTYWAVRNFQEKFGMKPVDGLVGAKTKQVLISKSKYYREYVMEQIQKGNTFTH
ECCEECHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YGNVPLKNQTKPRAGTQKPAGTNKPAQKPQNKTGGQSNQKQNTTAANMPGGFSNNDINLL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHH
AQAVYGEARGEPFEGQVAIAAVILNRIKSPLFPNTVAGVIFEPLAFTAVADGQIYMQPNE
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEECCEEEECCCH
RAKEAVLDAINGWDPSENAIYYFNPDTATSKWIWGRPQIKRIGKHIFCE
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8830707; 8760912; 9384377; 10197998; 10658652 [H]