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The map label for this gene is yclA [H]

Identifier: 154684848

GI number: 154684848

Start: 377039

End: 377911

Strand: Reverse

Name: yclA [H]

Synonym: RBAM_003790

Alternate gene names: 154684848

Gene position: 377911-377039 (Counterclockwise)

Preceding gene: 154684853

Following gene: 154684847

Centisome position: 9.64

GC content: 48.34

Gene sequence:

>873_bases
ATGGATATCCGGCAATTGCGTTATTTCATCACCATTGCCCAAGAGCAGAAAATCACGACGGCCGCCAAAAAGCTTCATAT
GGCACAGCCGCCTTTAAGCAGACAGCTGAAACAGCTCGAAGAAGAATTGGGCGTCATTCTGTTTGACCGCAATAAAAAGA
AACAAATGACCCTTACATATGAAGGAAATGTCTTTTTGAAAAGAGCGAAAGAGATTCTTCATAAGTTTGAGGACGCTATA
ATCGAGGTACAAGAGCTGAAGGAGGAGGTGGCCGGGACACTTGCCGTCGGGTCAACCATTTACTGCGCCGCGCTCATGCT
TGAGAAAGTGTCACAGATGAAAGAGCGGTTTCCGCATCTCACCTTTAACATTTGGGAAGATGAGCCCGTCTCACTGCTCG
AGCTTCTGGAAACCCGGCAGATTGACGCCGCCGTCACCACCACCTTGATTAAAAACGACGGCATTGAATCGAAGGAGCTT
GAAGACATTCCGTGTGTATTGGTGCTGTCCGAGCATTCAAAACGACTGTGCGGTGAAACGATTAAACTGAATGACATCGC
CTCTTTGCCTTTGATCCTTTTGCGCACCGTCAATGGAAGAGGTGTGTATGACCAAGTCCTCCAAGTTTGTGAACAGTACG
GTCTCGACCTTCAGGTTGTCTGTGAATGCCATGACTCCGCGACGCTCTTGAGCCTCGTATCATCAGGGTTCGGCGCCACA
ATTCTGCCTTTGACGATGATTCCCGTCCATATGCGTTCGCATGTCAAAACGGTTATCATCGAAGACAACCCGTTTGTCAT
GACACCGGCCGTCATCTGGAGAAAAGACAGCTATCTGCCGAAGCCGGTCAGACAGTTTTTGGAATTATTTTGA

Upstream 100 bases:

>100_bases
TGGTGTCAAAACCATCATACTCCTGATATATCCATATGAAAAATATTCGTTAAATCTATGTATCATACCTTAAAGGTATA
AAAGAAAGGAGCGCGTTCTC

Downstream 100 bases:

>100_bases
TCCGGAACACGAAGAATTCCCGCTTATGAAAACCAATTACTTGACTCATACTAAAAAAAAGTTATCCTTTACAATTGGGA
GCTATTTTTTCTCAATAAAA

Product: YclA

Products: NA

Alternate protein names: Bsd operon regulatory protein [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDIRQLRYFITIAQEQKITTAAKKLHMAQPPLSRQLKQLEEELGVILFDRNKKKQMTLTYEGNVFLKRAKEILHKFEDAI
IEVQELKEEVAGTLAVGSTIYCAALMLEKVSQMKERFPHLTFNIWEDEPVSLLELLETRQIDAAVTTTLIKNDGIESKEL
EDIPCVLVLSEHSKRLCGETIKLNDIASLPLILLRTVNGRGVYDQVLQVCEQYGLDLQVVCECHDSATLLSLVSSGFGAT
ILPLTMIPVHMRSHVKTVIIEDNPFVMTPAVIWRKDSYLPKPVRQFLELF

Sequences:

>Translated_290_residues
MDIRQLRYFITIAQEQKITTAAKKLHMAQPPLSRQLKQLEEELGVILFDRNKKKQMTLTYEGNVFLKRAKEILHKFEDAI
IEVQELKEEVAGTLAVGSTIYCAALMLEKVSQMKERFPHLTFNIWEDEPVSLLELLETRQIDAAVTTTLIKNDGIESKEL
EDIPCVLVLSEHSKRLCGETIKLNDIASLPLILLRTVNGRGVYDQVLQVCEQYGLDLQVVCECHDSATLLSLVSSGFGAT
ILPLTMIPVHMRSHVKTVIIEDNPFVMTPAVIWRKDSYLPKPVRQFLELF
>Mature_290_residues
MDIRQLRYFITIAQEQKITTAAKKLHMAQPPLSRQLKQLEEELGVILFDRNKKKQMTLTYEGNVFLKRAKEILHKFEDAI
IEVQELKEEVAGTLAVGSTIYCAALMLEKVSQMKERFPHLTFNIWEDEPVSLLELLETRQIDAAVTTTLIKNDGIESKEL
EDIPCVLVLSEHSKRLCGETIKLNDIASLPLILLRTVNGRGVYDQVLQVCEQYGLDLQVVCECHDSATLLSLVSSGFGAT
ILPLTMIPVHMRSHVKTVIIEDNPFVMTPAVIWRKDSYLPKPVRQFLELF

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=299, Percent_Identity=26.4214046822742, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=26.027397260274, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=26.5060240963855, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI2367136, Length=252, Percent_Identity=27.3809523809524, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788297, Length=251, Percent_Identity=29.4820717131474, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693105, Length=291, Percent_Identity=24.3986254295533, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789506, Length=289, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788887, Length=261, Percent_Identity=28.735632183908, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787530, Length=253, Percent_Identity=25.296442687747, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=30.1369863013699, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788748, Length=258, Percent_Identity=24.031007751938, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789204, Length=250, Percent_Identity=23.6, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787806, Length=302, Percent_Identity=24.5033112582781, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788296, Length=246, Percent_Identity=25.609756097561, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33004; Mature: 33004

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRQLRYFITIAQEQKITTAAKKLHMAQPPLSRQLKQLEEELGVILFDRNKKKQMTLTY
CCHHHEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEEEE
EGNVFLKRAKEILHKFEDAIIEVQELKEEVAGTLAVGSTIYCAALMLEKVSQMKERFPHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFNIWEDEPVSLLELLETRQIDAAVTTTLIKNDGIESKELEDIPCVLVLSEHSKRLCGET
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHCCCC
IKLNDIASLPLILLRTVNGRGVYDQVLQVCEQYGLDLQVVCECHDSATLLSLVSSGFGAT
EEECCHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCH
ILPLTMIPVHMRSHVKTVIIEDNPFVMTPAVIWRKDSYLPKPVRQFLELF
HHHHHHHHHHHHCCEEEEEECCCCEEECCHHEEECCCCCCHHHHHHHHHC
>Mature Secondary Structure
MDIRQLRYFITIAQEQKITTAAKKLHMAQPPLSRQLKQLEEELGVILFDRNKKKQMTLTY
CCHHHEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEEEE
EGNVFLKRAKEILHKFEDAIIEVQELKEEVAGTLAVGSTIYCAALMLEKVSQMKERFPHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TFNIWEDEPVSLLELLETRQIDAAVTTTLIKNDGIESKELEDIPCVLVLSEHSKRLCGET
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHCCCC
IKLNDIASLPLILLRTVNGRGVYDQVLQVCEQYGLDLQVVCECHDSATLLSLVSSGFGAT
EEECCHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCH
ILPLTMIPVHMRSHVKTVIIEDNPFVMTPAVIWRKDSYLPKPVRQFLELF
HHHHHHHHHHHHCCEEEEEECCCCEEECCHHEEECCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]