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The map label for this gene is yckK [H]

Identifier: 154684847

GI number: 154684847

Start: 376113

End: 376910

Strand: Reverse

Name: yckK [H]

Synonym: RBAM_003780

Alternate gene names: 154684847

Gene position: 376910-376113 (Counterclockwise)

Preceding gene: 154684848

Following gene: 154684846

Centisome position: 9.62

GC content: 45.49

Gene sequence:

>798_bases
ATGAAAAAAGCATTATTCGCTTTGTTCATGATGGTCAGCATCGCAATGCTTGCAGCCTGCGGAAACTCACAGGACAGCGG
ATCTAAGTCAAAAGACGGCGATCTTTGGGCTTCTGTCAAGAAAAAAGGCGTACTGACCGTCGGTACAGAAGGAACGTACG
AACCGTTTACCTTCCATGATAAAAAGACTGACAAACTGACCGGATATGATGTTGAAGTGATTCAAGAAGTCGCGAAACGC
CTCGGTCTGAAAGCTGACTTCAAAGAAACGCAATGGGACAGCATGTTTGCCGGATTAAACGCGAAACGGTTTGACGTCGT
TGCCAACCAAGTCGGCAAAACAGGCCGTGAGAACCAATATGATTTTTCGGATAAATACACAACGTCAAATGCAGTCGTCG
TGACGAAAAAAGACAGCACGATTGCGAAAGAGGCTGATGTCAAAGGAAAAACAGCGGCTCAGTCATTAACAAGCAATTAC
AATAAATTAGCGAAAGAAGCCGGCGCGAAAATTGAAGGCGTTGAAGGCATGGCTCAATCCCTTCAGCTCATTCAGCAGGG
CCGCGCTGATCTGACTTACAACGATAAGCTTGCCGTACTGAACTACTTAAAAACGTCCGGCAATAAAAACGTGAAAATCG
CGTTTGAAGCAGGCCAGCCGCAGCAGACGTATTTCGCATTCCGAAAAGGAAGCGGCGAACTTGTGACTCACGTCAACAAA
GCGTTAAAAGAAATGAAAGAGGACGGGACTCTTTCTAAAATAGCGAAAAAATGGTTCGGCGAAGATGTTTCTAAGTAA

Upstream 100 bases:

>100_bases
AAAACCAATTACTTGACTCATACTAAAAAAAAGTTATCCTTTACAATTGGGAGCTATTTTTTCTCAATAAAACTACTCAG
AAAAGTGGGGAATAAAGAAG

Downstream 100 bases:

>100_bases
CATGCCGGAACCCGTGATTGCAACAGCCATTCCTTGGGATCTGGTGCAGCAGTCATTCTGGCCGATTCTTTCCGGGGGAA
TCTATTACTCGATTCCCCTT

Product: YckK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKKALFALFMMVSIAMLAACGNSQDSGSKSKDGDLWASVKKKGVLTVGTEGTYEPFTFHDKKTDKLTGYDVEVIQEVAKR
LGLKADFKETQWDSMFAGLNAKRFDVVANQVGKTGRENQYDFSDKYTTSNAVVVTKKDSTIAKEADVKGKTAAQSLTSNY
NKLAKEAGAKIEGVEGMAQSLQLIQQGRADLTYNDKLAVLNYLKTSGNKNVKIAFEAGQPQQTYFAFRKGSGELVTHVNK
ALKEMKEDGTLSKIAKKWFGEDVSK

Sequences:

>Translated_265_residues
MKKALFALFMMVSIAMLAACGNSQDSGSKSKDGDLWASVKKKGVLTVGTEGTYEPFTFHDKKTDKLTGYDVEVIQEVAKR
LGLKADFKETQWDSMFAGLNAKRFDVVANQVGKTGRENQYDFSDKYTTSNAVVVTKKDSTIAKEADVKGKTAAQSLTSNY
NKLAKEAGAKIEGVEGMAQSLQLIQQGRADLTYNDKLAVLNYLKTSGNKNVKIAFEAGQPQQTYFAFRKGSGELVTHVNK
ALKEMKEDGTLSKIAKKWFGEDVSK
>Mature_265_residues
MKKALFALFMMVSIAMLAACGNSQDSGSKSKDGDLWASVKKKGVLTVGTEGTYEPFTFHDKKTDKLTGYDVEVIQEVAKR
LGLKADFKETQWDSMFAGLNAKRFDVVANQVGKTGRENQYDFSDKYTTSNAVVVTKKDSTIAKEADVKGKTAAQSLTSNY
NKLAKEAGAKIEGVEGMAQSLQLIQQGRADLTYNDKLAVLNYLKTSGNKNVKIAFEAGQPQQTYFAFRKGSGELVTHVNK
ALKEMKEDGTLSKIAKKWFGEDVSK

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=264, Percent_Identity=38.2575757575758, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1787031, Length=220, Percent_Identity=32.2727272727273, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788649, Length=273, Percent_Identity=27.8388278388278, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=28.1385281385281, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787085, Length=227, Percent_Identity=27.3127753303965, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787088, Length=225, Percent_Identity=27.5555555555556, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29075; Mature: 29075

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALFALFMMVSIAMLAACGNSQDSGSKSKDGDLWASVKKKGVLTVGTEGTYEPFTFHD
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCEEECC
KKTDKLTGYDVEVIQEVAKRLGLKADFKETQWDSMFAGLNAKRFDVVANQVGKTGRENQY
CCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
DFSDKYTTSNAVVVTKKDSTIAKEADVKGKTAAQSLTSNYNKLAKEAGAKIEGVEGMAQS
CCCCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LQLIQQGRADLTYNDKLAVLNYLKTSGNKNVKIAFEAGQPQQTYFAFRKGSGELVTHVNK
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHEEECCCCHHHHHHHH
ALKEMKEDGTLSKIAKKWFGEDVSK
HHHHHHCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKALFALFMMVSIAMLAACGNSQDSGSKSKDGDLWASVKKKGVLTVGTEGTYEPFTFHD
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCEEECC
KKTDKLTGYDVEVIQEVAKRLGLKADFKETQWDSMFAGLNAKRFDVVANQVGKTGRENQY
CCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
DFSDKYTTSNAVVVTKKDSTIAKEADVKGKTAAQSLTSNYNKLAKEAGAKIEGVEGMAQS
CCCCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LQLIQQGRADLTYNDKLAVLNYLKTSGNKNVKIAFEAGQPQQTYFAFRKGSGELVTHVNK
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHEEECCCCHHHHHHHH
ALKEMKEDGTLSKIAKKWFGEDVSK
HHHHHHCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]