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The map label for this gene is yckJ [H]

Identifier: 154684846

GI number: 154684846

Start: 375422

End: 376111

Strand: Reverse

Name: yckJ [H]

Synonym: RBAM_003770

Alternate gene names: 154684846

Gene position: 376111-375422 (Counterclockwise)

Preceding gene: 154684847

Following gene: 154684845

Centisome position: 9.6

GC content: 48.41

Gene sequence:

>690_bases
ATGCCGGAACCCGTGATTGCAACAGCCATTCCTTGGGATCTGGTGCAGCAGTCATTCTGGCCGATTCTTTCCGGGGGAAT
CTATTACTCGATTCCCCTTACCATTCTTTCCTTTATTTTCGGAATGATCATCGCGCTGATTACGGCGCTCGCCAGAATGT
CAAATATGAAACCATTGAGATGGGTGTTCAGCGTATACGTATCAGCGGTGCGCGGCACTCCTCTTCTCGTTCAATTGTTC
ATCATTTTTTATCTGTTCCCGACATTTAATATTACGCTGGACCCTTTCCCAAGCGCAGTCATCGCGTTTTCATTAAACGT
GGGCGCCTACGCTTCTGAAATCATCCGGGCATCTATTTCATCCGTGCCGAAAGGGCAATGGGAAGCCGGTTATACGATCG
GCATGACCTATCGCAAGACATTGTTCCGCGTGATCCTGCCGCAGGCGTTTCGCGTGTCCATTCCGCCATTATCAAATACA
TTTATCAGCCTGATCAAGGATACATCTCTCGCGTCACAGATTCTCGTCGCGGAGCTGTTCAGAAAAGCGCAGGAAATCGG
CGCGCAAAACCTTGATCAAATCTTGGTCATCTATATTGAAGCCGCCTTTATTTATTGGATCATCTGCTTCTTGCTGTCAA
TCGTACAGCACGTGATCGAACGGCGTCTCGACCGTTATGTGGCCAAATAA

Upstream 100 bases:

>100_bases
CTTGTGACTCACGTCAACAAAGCGTTAAAAGAAATGAAAGAGGACGGGACTCTTTCTAAAATAGCGAAAAAATGGTTCGG
CGAAGATGTTTCTAAGTAAC

Downstream 100 bases:

>100_bases
GGAGGTTTCGATATGCTTTCCGTCAAAGGATTAAATAAATCATTCGGACAGAATGAAATTTTAAAAAAGATAGATATGGA
GATAGAAAAAGGGAAAGTCA

Product: YckJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPEPVIATAIPWDLVQQSFWPILSGGIYYSIPLTILSFIFGMIIALITALARMSNMKPLRWVFSVYVSAVRGTPLLVQLF
IIFYLFPTFNITLDPFPSAVIAFSLNVGAYASEIIRASISSVPKGQWEAGYTIGMTYRKTLFRVILPQAFRVSIPPLSNT
FISLIKDTSLASQILVAELFRKAQEIGAQNLDQILVIYIEAAFIYWIICFLLSIVQHVIERRLDRYVAK

Sequences:

>Translated_229_residues
MPEPVIATAIPWDLVQQSFWPILSGGIYYSIPLTILSFIFGMIIALITALARMSNMKPLRWVFSVYVSAVRGTPLLVQLF
IIFYLFPTFNITLDPFPSAVIAFSLNVGAYASEIIRASISSVPKGQWEAGYTIGMTYRKTLFRVILPQAFRVSIPPLSNT
FISLIKDTSLASQILVAELFRKAQEIGAQNLDQILVIYIEAAFIYWIICFLLSIVQHVIERRLDRYVAK
>Mature_228_residues
PEPVIATAIPWDLVQQSFWPILSGGIYYSIPLTILSFIFGMIIALITALARMSNMKPLRWVFSVYVSAVRGTPLLVQLFI
IFYLFPTFNITLDPFPSAVIAFSLNVGAYASEIIRASISSVPKGQWEAGYTIGMTYRKTLFRVILPQAFRVSIPPLSNTF
ISLIKDTSLASQILVAELFRKAQEIGAQNLDQILVIYIEAAFIYWIICFLLSIVQHVIERRLDRYVAK

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788226, Length=205, Percent_Identity=51.7073170731707, Blast_Score=211, Evalue=3e-56,
Organism=Escherichia coli, GI1787030, Length=206, Percent_Identity=38.8349514563107, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1787086, Length=215, Percent_Identity=32.5581395348837, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI1786873, Length=210, Percent_Identity=34.2857142857143, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1787087, Length=234, Percent_Identity=32.0512820512821, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI87082239, Length=185, Percent_Identity=35.1351351351351, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1788645, Length=212, Percent_Identity=32.0754716981132, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788646, Length=216, Percent_Identity=26.8518518518519, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786874, Length=174, Percent_Identity=28.1609195402299, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48994930, Length=123, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25847; Mature: 25716

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEPVIATAIPWDLVQQSFWPILSGGIYYSIPLTILSFIFGMIIALITALARMSNMKPLR
CCCCCEECCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WVFSVYVSAVRGTPLLVQLFIIFYLFPTFNITLDPFPSAVIAFSLNVGAYASEIIRASIS
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHEECCHHHHHHHHHHHHHH
SVPKGQWEAGYTIGMTYRKTLFRVILPQAFRVSIPPLSNTFISLIKDTSLASQILVAELF
HCCCCCCCCCCEEHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RKAQEIGAQNLDQILVIYIEAAFIYWIICFLLSIVQHVIERRLDRYVAK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PEPVIATAIPWDLVQQSFWPILSGGIYYSIPLTILSFIFGMIIALITALARMSNMKPLR
CCCCEECCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WVFSVYVSAVRGTPLLVQLFIIFYLFPTFNITLDPFPSAVIAFSLNVGAYASEIIRASIS
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHEECCHHHHHHHHHHHHHH
SVPKGQWEAGYTIGMTYRKTLFRVILPQAFRVSIPPLSNTFISLIKDTSLASQILVAELF
HCCCCCCCCCCEEHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
RKAQEIGAQNLDQILVIYIEAAFIYWIICFLLSIVQHVIERRLDRYVAK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]