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The map label for this gene is nasC [H]

Identifier: 154684821

GI number: 154684821

Start: 328172

End: 330304

Strand: Reverse

Name: nasC [H]

Synonym: RBAM_003520

Alternate gene names: 154684821

Gene position: 330304-328172 (Counterclockwise)

Preceding gene: 154684822

Following gene: 154684820

Centisome position: 8.43

GC content: 50.16

Gene sequence:

>2133_bases
TTGACTGAACCGATGCTCCGTTATTTCCGTGACAAACTGCAAGATATTCAATCGGAAAAAACATATGAGACGCAATGCCC
GTTCTGCAGCATGCAATGCAAAATGCAGCTTACGGAACAAACCTTCGCGGCACGGAAGAAATACACTGCAATCGGCATTG
ATAATCCGACGACACATGGCCGTTTATGTACAAAAGGCATGAATGCCCATCAGCATGCCGTGCACTCTTCCCGCATTACA
CGCCCCCTGCTTAAAAAAAACGGTGAATTTGTGCCTGTCTCTTGGGCTGAAGCACTGAATCGCATTAAGGAACAAATCAA
TCTGATCCAAACCGAACACGGCAATGATGCGATGGCTGTCTACGGGAGCGCCTCAATTACAAATGAAGAAGCCTATCTGC
TTGGAAAGTTCGCACGGGTCGGCTTACAGACGAAATACATCGATTACAACGGGCGGCTCTGTATGTCTGCCGCAGCAACG
GCGGCCGGCCAGACATTCGGCGCGGACAGGGGCCTGACGAATCCATTATCGGACATCCCGCATACACGTGTCATCATCCT
GGCAGGCACCAATATCGCCGAATGCCAGCCGACGATCATGCCATATTTTGATAAAGCGAAAGAAAACGGGGCTTACATTA
TCGCCATTGATCCCCGGGAAACGGCGACGACCAAAATAGCCGATCTTCACCTGAAAATCAAGCCCGGTACAGACGCCGCC
TTGGCAAACGGACTCGTCAAAATCTGCATTGACGAACAGCTTACAGACAAAGACTTTATCCGCACACGAACAAACGGATT
CAATAAGCTCAAACGGCATATCGACGCTTTGGATCTGAAAGAGATTTCCGAGCAGACAAGTGTTCCTTTGGAGCATATAC
GAAAGGCCGCCGTGAAGTTTGCCAAGGAAACATCCGGCATGCTGTTTACAGCGCGGGGGATCGAACAGCAGACTGACGGT
ACCGCGGCAGTGAAAGCTTTCTTAAATATGGTGCTGATCACCGGAAAAATCGGCAAGCCCTTCTCAGGTTACGGGGCGAT
TACCGGTCAAGGCAACGGACAAGGCGCGAGAGAACACGGCCAAAAAGCGGATCAGCTTCCGGGCTACCGTTCTATCGAAA
TTGAAGAACACCGGGCGTATATTGCGAAGGTGTGGGGGATTCATCAGGATGAGCTGCCGCGAAAAGGCGTCTCAGCCTAT
GAAATGATAGAAAAAATAGATAGCGGCGACATCAAAGGACTGTTTCTCATGTGTTCCAATCCGGCTGTTTCCGGCCCAAA
TGCGAATTTCGTCAAGCAGGCAATCAAGAAACTGAGATTCTTTGTCGCAGTTGATTTATTTATTTCTGAGACCGCGGCAT
TCGCGGATGTGATTTTACCCGCCTCCTCTTATTTGGAAGACGAAGGAACGATGACAAATGTAGAAGGCCGCGTGACATTA
AGAGAAGCGAGCCGCACCTGCCCCGGAGAAGCGAAACATGATTGGCAGATCATCTGTGACATTGCCTCGGCTCTTGGTAA
AGGGCATTATTTTCCCTATACGTCGGCGGAGGAAATCTTTAACGAACTGAGAGAAGCGAGCCGCGGCGGAATTGCTGATT
ACTCTGGCATCACCTATGACAGGCTCAGGCGGGAAGGCGGCATTCAGTGGCCATGTCCTGAAGCGGATCATCCCGGAACC
GGGCGATTGTTTGAGCACTCATTTGCCCATCCCGATCACAGAGCTGAATTAAGCGTGATTCCAAATGAACCGGCCGTCCC
GAAAGAGAAACCGAGCGCCGGCTATCCGCTTTATTTAACCACAGGAAGGGTGATGTCTCATTACTTAACGGGTGTACAAA
CGAGAAAAAGCTCCGCCCTGGCTGCAAGACATTTCGAATCCTTTGTGGAAATCCATCCAAAAACGGCGGCCGACTATCAT
ATCGAAGACCGTGTTCTCGTGAAAATAGAATCCCCCCGGGGCAGCATCACGGTCCGGAGCAGATGGTCGGAGAAAGTACG
AAAAGACACGTTGTTTGTTCCCATCCATTGGTCTGACACGCAAAACGTCAACGATTTAATTGGTGAAGCCTTGGACCCGG
ACTGTAAAATGCCCGGTTTTAAGATGTGTGCGGTACGAATCAGCCCTATATAA

Upstream 100 bases:

>100_bases
GAAGATATGCGGACACAGCTTCTGAAAAATCTCCGTTCAGACATATCCCTGCTGCAAAACCTGTCTGCCGGGAAAACGCC
ATACGAAAGGATGAAGCTTT

Downstream 100 bases:

>100_bases
TTTTTCATAAAATTTTTAGATTTTTTAGTATTTTTTGTTACATTTCATAACATGCACCTTAAATATTTGTTTTTGCTGTG
CTACGCTAAAGAAAACATAG

Product: NasC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MTEPMLRYFRDKLQDIQSEKTYETQCPFCSMQCKMQLTEQTFAARKKYTAIGIDNPTTHGRLCTKGMNAHQHAVHSSRIT
RPLLKKNGEFVPVSWAEALNRIKEQINLIQTEHGNDAMAVYGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAAT
AAGQTFGADRGLTNPLSDIPHTRVIILAGTNIAECQPTIMPYFDKAKENGAYIIAIDPRETATTKIADLHLKIKPGTDAA
LANGLVKICIDEQLTDKDFIRTRTNGFNKLKRHIDALDLKEISEQTSVPLEHIRKAAVKFAKETSGMLFTARGIEQQTDG
TAAVKAFLNMVLITGKIGKPFSGYGAITGQGNGQGAREHGQKADQLPGYRSIEIEEHRAYIAKVWGIHQDELPRKGVSAY
EMIEKIDSGDIKGLFLMCSNPAVSGPNANFVKQAIKKLRFFVAVDLFISETAAFADVILPASSYLEDEGTMTNVEGRVTL
REASRTCPGEAKHDWQIICDIASALGKGHYFPYTSAEEIFNELREASRGGIADYSGITYDRLRREGGIQWPCPEADHPGT
GRLFEHSFAHPDHRAELSVIPNEPAVPKEKPSAGYPLYLTTGRVMSHYLTGVQTRKSSALAARHFESFVEIHPKTAADYH
IEDRVLVKIESPRGSITVRSRWSEKVRKDTLFVPIHWSDTQNVNDLIGEALDPDCKMPGFKMCAVRISPI

Sequences:

>Translated_710_residues
MTEPMLRYFRDKLQDIQSEKTYETQCPFCSMQCKMQLTEQTFAARKKYTAIGIDNPTTHGRLCTKGMNAHQHAVHSSRIT
RPLLKKNGEFVPVSWAEALNRIKEQINLIQTEHGNDAMAVYGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAAT
AAGQTFGADRGLTNPLSDIPHTRVIILAGTNIAECQPTIMPYFDKAKENGAYIIAIDPRETATTKIADLHLKIKPGTDAA
LANGLVKICIDEQLTDKDFIRTRTNGFNKLKRHIDALDLKEISEQTSVPLEHIRKAAVKFAKETSGMLFTARGIEQQTDG
TAAVKAFLNMVLITGKIGKPFSGYGAITGQGNGQGAREHGQKADQLPGYRSIEIEEHRAYIAKVWGIHQDELPRKGVSAY
EMIEKIDSGDIKGLFLMCSNPAVSGPNANFVKQAIKKLRFFVAVDLFISETAAFADVILPASSYLEDEGTMTNVEGRVTL
REASRTCPGEAKHDWQIICDIASALGKGHYFPYTSAEEIFNELREASRGGIADYSGITYDRLRREGGIQWPCPEADHPGT
GRLFEHSFAHPDHRAELSVIPNEPAVPKEKPSAGYPLYLTTGRVMSHYLTGVQTRKSSALAARHFESFVEIHPKTAADYH
IEDRVLVKIESPRGSITVRSRWSEKVRKDTLFVPIHWSDTQNVNDLIGEALDPDCKMPGFKMCAVRISPI
>Mature_709_residues
TEPMLRYFRDKLQDIQSEKTYETQCPFCSMQCKMQLTEQTFAARKKYTAIGIDNPTTHGRLCTKGMNAHQHAVHSSRITR
PLLKKNGEFVPVSWAEALNRIKEQINLIQTEHGNDAMAVYGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAATA
AGQTFGADRGLTNPLSDIPHTRVIILAGTNIAECQPTIMPYFDKAKENGAYIIAIDPRETATTKIADLHLKIKPGTDAAL
ANGLVKICIDEQLTDKDFIRTRTNGFNKLKRHIDALDLKEISEQTSVPLEHIRKAAVKFAKETSGMLFTARGIEQQTDGT
AAVKAFLNMVLITGKIGKPFSGYGAITGQGNGQGAREHGQKADQLPGYRSIEIEEHRAYIAKVWGIHQDELPRKGVSAYE
MIEKIDSGDIKGLFLMCSNPAVSGPNANFVKQAIKKLRFFVAVDLFISETAAFADVILPASSYLEDEGTMTNVEGRVTLR
EASRTCPGEAKHDWQIICDIASALGKGHYFPYTSAEEIFNELREASRGGIADYSGITYDRLRREGGIQWPCPEADHPGTG
RLFEHSFAHPDHRAELSVIPNEPAVPKEKPSAGYPLYLTTGRVMSHYLTGVQTRKSSALAARHFESFVEIHPKTAADYHI
EDRVLVKIESPRGSITVRSRWSEKVRKDTLFVPIHWSDTQNVNDLIGEALDPDCKMPGFKMCAVRISPI

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=700, Percent_Identity=31.5714285714286, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788534, Length=794, Percent_Identity=25.3148614609572, Blast_Score=233, Evalue=4e-62,
Organism=Escherichia coli, GI3868719, Length=616, Percent_Identity=23.0519480519481, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI3868720, Length=620, Percent_Identity=23.5483870967742, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI171474008, Length=561, Percent_Identity=24.0641711229947, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1787778, Length=519, Percent_Identity=23.3140655105973, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787870, Length=571, Percent_Identity=23.1173380035026, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI87081797, Length=562, Percent_Identity=23.1316725978648, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87081994, Length=503, Percent_Identity=23.8568588469185, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 78738; Mature: 78606

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPMLRYFRDKLQDIQSEKTYETQCPFCSMQCKMQLTEQTFAARKKYTAIGIDNPTTHG
CCCHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHCEEEEECCCCCCCC
RLCTKGMNAHQHAVHSSRITRPLLKKNGEFVPVSWAEALNRIKEQINLIQTEHGNDAMAV
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHEECCCCCEEEE
YGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAATAAGQTFGADRGLTNPLSDIP
EECCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEHHHHHHCCCCCCCCCCCCCCHHHCC
HTRVIILAGTNIAECQPTIMPYFDKAKENGAYIIAIDPRETATTKIADLHLKIKPGTDAA
CCEEEEEECCCHHCCCCCCCCCHHHHCCCCEEEEEECCCCCCCEEEEEEEEEECCCCCHH
LANGLVKICIDEQLTDKDFIRTRTNGFNKLKRHIDALDLKEISEQTSVPLEHIRKAAVKF
HHCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AKETSGMLFTARGIEQQTDGTAAVKAFLNMVLITGKIGKPFSGYGAITGQGNGQGAREHG
HHHCCCEEEEECCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEECCCCCCCHHHHC
QKADQLPGYRSIEIEEHRAYIAKVWGIHQDELPRKGVSAYEMIEKIDSGDIKGLFLMCSN
CHHHHCCCCEEEEEHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECC
PAVSGPNANFVKQAIKKLRFFVAVDLFISETAAFADVILPASSYLEDEGTMTNVEGRVTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEECCCCEEEE
REASRTCPGEAKHDWQIICDIASALGKGHYFPYTSAEEIFNELREASRGGIADYSGITYD
EHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHH
RLRREGGIQWPCPEADHPGTGRLFEHSFAHPDHRAELSVIPNEPAVPKEKPSAGYPLYLT
HHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
TGRVMSHYLTGVQTRKSSALAARHFESFVEIHPKTAADYHIEDRVLVKIESPRGSITVRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEEECCCCCEEEHH
RWSEKVRKDTLFVPIHWSDTQNVNDLIGEALDPDCKMPGFKMCAVRISPI
HHHHHHHHCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TEPMLRYFRDKLQDIQSEKTYETQCPFCSMQCKMQLTEQTFAARKKYTAIGIDNPTTHG
CCHHHHHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHCEEEEECCCCCCCC
RLCTKGMNAHQHAVHSSRITRPLLKKNGEFVPVSWAEALNRIKEQINLIQTEHGNDAMAV
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHEECCCCCEEEE
YGSASITNEEAYLLGKFARVGLQTKYIDYNGRLCMSAAATAAGQTFGADRGLTNPLSDIP
EECCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEHHHHHHCCCCCCCCCCCCCCHHHCC
HTRVIILAGTNIAECQPTIMPYFDKAKENGAYIIAIDPRETATTKIADLHLKIKPGTDAA
CCEEEEEECCCHHCCCCCCCCCHHHHCCCCEEEEEECCCCCCCEEEEEEEEEECCCCCHH
LANGLVKICIDEQLTDKDFIRTRTNGFNKLKRHIDALDLKEISEQTSVPLEHIRKAAVKF
HHCCEEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AKETSGMLFTARGIEQQTDGTAAVKAFLNMVLITGKIGKPFSGYGAITGQGNGQGAREHG
HHHCCCEEEEECCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEECCCCCCCHHHHC
QKADQLPGYRSIEIEEHRAYIAKVWGIHQDELPRKGVSAYEMIEKIDSGDIKGLFLMCSN
CHHHHCCCCEEEEEHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECC
PAVSGPNANFVKQAIKKLRFFVAVDLFISETAAFADVILPASSYLEDEGTMTNVEGRVTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEECCCCEEEE
REASRTCPGEAKHDWQIICDIASALGKGHYFPYTSAEEIFNELREASRGGIADYSGITYD
EHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHH
RLRREGGIQWPCPEADHPGTGRLFEHSFAHPDHRAELSVIPNEPAVPKEKPSAGYPLYLT
HHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEE
TGRVMSHYLTGVQTRKSSALAARHFESFVEIHPKTAADYHIEDRVLVKIESPRGSITVRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEEECCCCCEEEHH
RWSEKVRKDTLFVPIHWSDTQNVNDLIGEALDPDCKMPGFKMCAVRISPI
HHHHHHHHCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621 [H]