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The map label for this gene is nasB [H]

Identifier: 154684822

GI number: 154684822

Start: 330297

End: 332624

Strand: Reverse

Name: nasB [H]

Synonym: RBAM_003530

Alternate gene names: 154684822

Gene position: 332624-330297 (Counterclockwise)

Preceding gene: 154684829

Following gene: 154684821

Centisome position: 8.49

GC content: 50.26

Gene sequence:

>2328_bases
ATGAAGAAACAACGATTAATTGCAGCAGGGAACGGAATGGCCGGAATCCGCTGTATTGAACACATTTTAAAGATGAACCG
TGACATGTTTGAGATTGTCATTTTCGGAAGCGAACCTCATCCCAATTACAATCGCATTCTCTTATCCTCCGTTTTACAAG
GTGAGGTTTCGCTTGATGACATTACGCTGAATAGCCGCGAATGGTACGAAAAAAACGGAATTACGCTTTACACGGGAGAA
ACGGTTGTAGACATTGATACAGAACGGCAGGTTGTCACAACGGATCGGAAGCGCAGCATGACTTACGATCAAATCATCCT
TGCAACGGGCTCCTCTCCTTATATTCTCCCCATACCCGGAGCAGATAAAGAAGGTGTGTACGGCTTTCGGACAATAGAGG
ACTGCCAATCCCTTATCAATGCGGCCAGCCGGTATCAAAAGGCGGCGGTCATCGGCGCCGGTTTATTAGGTCTTGAAGCG
GCCGTCGGCTTACGGCAGCTCGGCATGGACGTCAGCGTCATTCACCACTCTTCCGCCATTATGCAAAAACAGCTTGATCA
AACGGCATCCCGCCTGCTGCAGAAAGAGCTGGAACGAAAAGGCTTAGTCTTTCTTTTAGAGAAAGGCACGGCATCCATTA
CAGGGAACTCACGGGCGGAAGGCATTCGTTTCAAAGACAGTTCTTCCATAGAAGCAGACCTGATTGTCATGGCCGCCGGT
GTGAGGCCCAATATCCGATTGGCTCCATCTGCCGGACTTTCAGTGAACAGAGGAATCATCGTCAATCAATTCATGCAGAC
GAACAAACCGAATGTGTATGCCGTCGGTGAATGCGCGGAGCATGACGGGATTGTATACGGATTGGCGCCGCCGCTTTATG
AACAGGGAAAAGTGCTCGCGCAGCATCTATGCGGCGTCCCGTGCGAAGGGTTTCAAGGCTTTGCACAATCTGCAGCCTTA
AAAATAGCCGGTATTGATGTATGGTCGGCAGGAAAGGTGCACGAGGATGAGCACACAGCCGGCATATTGTTCCATGATGA
GCATGCAGGCATCTACAAAAAAGCGCTCTTTGAGGACGATAAACCGGCCGGCTTTATTTTATTCGGGGACACGCGCGGTA
AACAGCGGCTTCTCGACAGTCTTGTGAAGCAAAGAGATATTTCCATCGTGAAAAAACAGCTCATGGAACCGGATCAGGGC
GGTATATCCTTTGAATCCATGCCTCCCCATGAAACCATTTGCCAATGCAGCTCAGTGACCAAGGGCTCCATTGAAGAAGC
GGTGAACAAATATGGCTTAAAAACCGCGGAGGAAGTGAAGCACATGACAAAAGCGTCCGGATCTTGCGGAGGATGCAGGC
CGCTCGTTGAAGACTTGCTGAAGTATATGGCGAGCGATGATTACACAAAACCCTCCCGCCAGCCTTCGTTTTGCGGCTGC
ACCGATTTGACAGAGGAAGAAGTCATCGCCGAAATTTGCCGCCGTCCGTTCACTGATGCCGCCGAGGTAATGAGAGAGAT
CGGCTGGAAAACAGAAAACGGCTGCCGCATTTGTATTCCGGCGCTTCACTATTACTTGGAAAGGACGCGGCCTGATTTTA
TGCAGTTCAATGAAATATCGGCAGAAGAGACATGCACCCTGATTCCGCAAATGTACGGCGGACTGACAAGTGCGGCAGAG
CTCAAAAACATCGCAGACGTCATCGAAGCATACGGCATCCCCGGGGTTTCGATGACTGACAGTCACCGGCTGAGGCTTTC
CGGAATCAGGCCAAATGACCTTGCAAGCATCAGAAAAGCACTGAACATGCCGGTTTTCTCCCCCCGGCACCGCTGGACGA
TTCAAATCAGAGCATGTACGTGCGGACAGCATCGCTCCTTTCAAGAGCTGGCAAGCAGGATTGAACGAGATACCGACACG
CTTGCCGTCTCCGCTTACGTATCCGTCAGCTTGTCATGTGAAAACAATTGCACCGATGCTTACATTCAGGATATCGGCGC
GATTCAGACACAAACTGGCTGGGAGATTTATATCGGGGGCTTCCGCGGAAAACAAGCCCGCGCCGGCAGCCTTTTTTGTG
TGACGGATAACGAAGACAGTACCGCCGCTATGATGAAAGGCCTTATTCAATATTATCGCGAAACGGCTCACTACAACGAA
GCGATTTATCAATGGGTTGACAGACTCGGCCTCGTCCATATCAGAGAAATGCTGTTTGAAGAAGATATGCGGACACAGCT
TCTGAAAAATCTCCGTTCAGACATATCCCTGCTGCAAAACCTGTCTGCCGGGAAAACGCCATACGAAAGGATGAAGCTTT
TTGACTGA

Upstream 100 bases:

>100_bases
TGTAAGATAATGCGACACATTTAATGCGTTAATCAGGCAATGTGCCATAATTTCTCATAGAGAAGCAATCTTACAGGCAG
CTGCAAAGGAGGTTTGGCGA

Downstream 100 bases:

>100_bases
ACCGATGCTCCGTTATTTCCGTGACAAACTGCAAGATATTCAATCGGAAAAAACATATGAGACGCAATGCCCGTTCTGCA
GCATGCAATGCAAAATGCAG

Product: NasB

Products: nitrite; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MKKQRLIAAGNGMAGIRCIEHILKMNRDMFEIVIFGSEPHPNYNRILLSSVLQGEVSLDDITLNSREWYEKNGITLYTGE
TVVDIDTERQVVTTDRKRSMTYDQIILATGSSPYILPIPGADKEGVYGFRTIEDCQSLINAASRYQKAAVIGAGLLGLEA
AVGLRQLGMDVSVIHHSSAIMQKQLDQTASRLLQKELERKGLVFLLEKGTASITGNSRAEGIRFKDSSSIEADLIVMAAG
VRPNIRLAPSAGLSVNRGIIVNQFMQTNKPNVYAVGECAEHDGIVYGLAPPLYEQGKVLAQHLCGVPCEGFQGFAQSAAL
KIAGIDVWSAGKVHEDEHTAGILFHDEHAGIYKKALFEDDKPAGFILFGDTRGKQRLLDSLVKQRDISIVKKQLMEPDQG
GISFESMPPHETICQCSSVTKGSIEEAVNKYGLKTAEEVKHMTKASGSCGGCRPLVEDLLKYMASDDYTKPSRQPSFCGC
TDLTEEEVIAEICRRPFTDAAEVMREIGWKTENGCRICIPALHYYLERTRPDFMQFNEISAEETCTLIPQMYGGLTSAAE
LKNIADVIEAYGIPGVSMTDSHRLRLSGIRPNDLASIRKALNMPVFSPRHRWTIQIRACTCGQHRSFQELASRIERDTDT
LAVSAYVSVSLSCENNCTDAYIQDIGAIQTQTGWEIYIGGFRGKQARAGSLFCVTDNEDSTAAMMKGLIQYYRETAHYNE
AIYQWVDRLGLVHIREMLFEEDMRTQLLKNLRSDISLLQNLSAGKTPYERMKLFD

Sequences:

>Translated_775_residues
MKKQRLIAAGNGMAGIRCIEHILKMNRDMFEIVIFGSEPHPNYNRILLSSVLQGEVSLDDITLNSREWYEKNGITLYTGE
TVVDIDTERQVVTTDRKRSMTYDQIILATGSSPYILPIPGADKEGVYGFRTIEDCQSLINAASRYQKAAVIGAGLLGLEA
AVGLRQLGMDVSVIHHSSAIMQKQLDQTASRLLQKELERKGLVFLLEKGTASITGNSRAEGIRFKDSSSIEADLIVMAAG
VRPNIRLAPSAGLSVNRGIIVNQFMQTNKPNVYAVGECAEHDGIVYGLAPPLYEQGKVLAQHLCGVPCEGFQGFAQSAAL
KIAGIDVWSAGKVHEDEHTAGILFHDEHAGIYKKALFEDDKPAGFILFGDTRGKQRLLDSLVKQRDISIVKKQLMEPDQG
GISFESMPPHETICQCSSVTKGSIEEAVNKYGLKTAEEVKHMTKASGSCGGCRPLVEDLLKYMASDDYTKPSRQPSFCGC
TDLTEEEVIAEICRRPFTDAAEVMREIGWKTENGCRICIPALHYYLERTRPDFMQFNEISAEETCTLIPQMYGGLTSAAE
LKNIADVIEAYGIPGVSMTDSHRLRLSGIRPNDLASIRKALNMPVFSPRHRWTIQIRACTCGQHRSFQELASRIERDTDT
LAVSAYVSVSLSCENNCTDAYIQDIGAIQTQTGWEIYIGGFRGKQARAGSLFCVTDNEDSTAAMMKGLIQYYRETAHYNE
AIYQWVDRLGLVHIREMLFEEDMRTQLLKNLRSDISLLQNLSAGKTPYERMKLFD
>Mature_775_residues
MKKQRLIAAGNGMAGIRCIEHILKMNRDMFEIVIFGSEPHPNYNRILLSSVLQGEVSLDDITLNSREWYEKNGITLYTGE
TVVDIDTERQVVTTDRKRSMTYDQIILATGSSPYILPIPGADKEGVYGFRTIEDCQSLINAASRYQKAAVIGAGLLGLEA
AVGLRQLGMDVSVIHHSSAIMQKQLDQTASRLLQKELERKGLVFLLEKGTASITGNSRAEGIRFKDSSSIEADLIVMAAG
VRPNIRLAPSAGLSVNRGIIVNQFMQTNKPNVYAVGECAEHDGIVYGLAPPLYEQGKVLAQHLCGVPCEGFQGFAQSAAL
KIAGIDVWSAGKVHEDEHTAGILFHDEHAGIYKKALFEDDKPAGFILFGDTRGKQRLLDSLVKQRDISIVKKQLMEPDQG
GISFESMPPHETICQCSSVTKGSIEEAVNKYGLKTAEEVKHMTKASGSCGGCRPLVEDLLKYMASDDYTKPSRQPSFCGC
TDLTEEEVIAEICRRPFTDAAEVMREIGWKTENGCRICIPALHYYLERTRPDFMQFNEISAEETCTLIPQMYGGLTSAAE
LKNIADVIEAYGIPGVSMTDSHRLRLSGIRPNDLASIRKALNMPVFSPRHRWTIQIRACTCGQHRSFQELASRIERDTDT
LAVSAYVSVSLSCENNCTDAYIQDIGAIQTQTGWEIYIGGFRGKQARAGSLFCVTDNEDSTAAMMKGLIQYYRETAHYNE
AIYQWVDRLGLVHIREMLFEEDMRTQLLKNLRSDISLLQNLSAGKTPYERMKLFD

Specific function: Required for nitrate assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=313, Percent_Identity=28.7539936102236, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI65787454, Length=313, Percent_Identity=28.7539936102236, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI226437568, Length=313, Percent_Identity=28.7539936102236, Blast_Score=114, Evalue=5e-25,
Organism=Homo sapiens, GI4757732, Length=221, Percent_Identity=29.8642533936652, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI22202629, Length=221, Percent_Identity=29.8642533936652, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1789765, Length=784, Percent_Identity=29.3367346938776, Blast_Score=296, Evalue=4e-81,
Organism=Escherichia coli, GI1789065, Length=304, Percent_Identity=28.2894736842105, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788892, Length=279, Percent_Identity=24.7311827956989, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1787352, Length=282, Percent_Identity=24.822695035461, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=304, Percent_Identity=23.6842105263158, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24639257, Length=311, Percent_Identity=24.4372990353698, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281359715, Length=311, Percent_Identity=24.4372990353698, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281359713, Length=311, Percent_Identity=24.4372990353698, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24639250, Length=311, Percent_Identity=24.4372990353698, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI18543267, Length=311, Percent_Identity=24.4372990353698, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24639252, Length=311, Percent_Identity=24.4372990353698, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24585130, Length=282, Percent_Identity=23.758865248227, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24581020, Length=219, Percent_Identity=25.5707762557078, Blast_Score=81, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28573993, Length=219, Percent_Identity=25.5707762557078, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21358499, Length=223, Percent_Identity=29.1479820627803, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640553, Length=224, Percent_Identity=29.9107142857143, Blast_Score=68, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640549, Length=224, Percent_Identity=29.9107142857143, Blast_Score=68, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640551, Length=224, Percent_Identity=29.9107142857143, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.7.1.4

Molecular weight: Translated: 86030; Mature: 86030

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQRLIAAGNGMAGIRCIEHILKMNRDMFEIVIFGSEPHPNYNRILLSSVLQGEVSLDD
CCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
ITLNSREWYEKNGITLYTGETVVDIDTERQVVTTDRKRSMTYDQIILATGSSPYILPIPG
EEECCHHHHHCCCEEEEECCEEEEECCCCEEEECCHHHCCCEEEEEEEECCCCEEEECCC
ADKEGVYGFRTIEDCQSLINAASRYQKAAVIGAGLLGLEAAVGLRQLGMDVSVIHHSSAI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHH
MQKQLDQTASRLLQKELERKGLVFLLEKGTASITGNSRAEGIRFKDSSSIEADLIVMAAG
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCEEECCCCCCCEEEEEEEEC
VRPNIRLAPSAGLSVNRGIIVNQFMQTNKPNVYAVGECAEHDGIVYGLAPPLYEQGKVLA
CCCCEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHH
QHLCGVPCEGFQGFAQSAALKIAGIDVWSAGKVHEDEHTAGILFHDEHAGIYKKALFEDD
HHHCCCCCCHHHHHHHHCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHCCCC
KPAGFILFGDTRGKQRLLDSLVKQRDISIVKKQLMEPDQGGISFESMPPHETICQCSSVT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHCCCC
KGSIEEAVNKYGLKTAEEVKHMTKASGSCGGCRPLVEDLLKYMASDDYTKPSRQPSFCGC
CCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
TDLTEEEVIAEICRRPFTDAAEVMREIGWKTENGCRICIPALHYYLERTRPDFMQFNEIS
CCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHCC
AEETCTLIPQMYGGLTSAAELKNIADVIEAYGIPGVSMTDSHRLRLSGIRPNDLASIRKA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHH
LNMPVFSPRHRWTIQIRACTCGQHRSFQELASRIERDTDTLAVSAYVSVSLSCENNCTDA
HCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHH
YIQDIGAIQTQTGWEIYIGGFRGKQARAGSLFCVTDNEDSTAAMMKGLIQYYRETAHYNE
HHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
AIYQWVDRLGLVHIREMLFEEDMRTQLLKNLRSDISLLQNLSAGKTPYERMKLFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
>Mature Secondary Structure
MKKQRLIAAGNGMAGIRCIEHILKMNRDMFEIVIFGSEPHPNYNRILLSSVLQGEVSLDD
CCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
ITLNSREWYEKNGITLYTGETVVDIDTERQVVTTDRKRSMTYDQIILATGSSPYILPIPG
EEECCHHHHHCCCEEEEECCEEEEECCCCEEEECCHHHCCCEEEEEEEECCCCEEEECCC
ADKEGVYGFRTIEDCQSLINAASRYQKAAVIGAGLLGLEAAVGLRQLGMDVSVIHHSSAI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHH
MQKQLDQTASRLLQKELERKGLVFLLEKGTASITGNSRAEGIRFKDSSSIEADLIVMAAG
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCEEECCCCCCCEEEEEEEEC
VRPNIRLAPSAGLSVNRGIIVNQFMQTNKPNVYAVGECAEHDGIVYGLAPPLYEQGKVLA
CCCCEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHH
QHLCGVPCEGFQGFAQSAALKIAGIDVWSAGKVHEDEHTAGILFHDEHAGIYKKALFEDD
HHHCCCCCCHHHHHHHHCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHCCCC
KPAGFILFGDTRGKQRLLDSLVKQRDISIVKKQLMEPDQGGISFESMPPHETICQCSSVT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHCCCC
KGSIEEAVNKYGLKTAEEVKHMTKASGSCGGCRPLVEDLLKYMASDDYTKPSRQPSFCGC
CCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
TDLTEEEVIAEICRRPFTDAAEVMREIGWKTENGCRICIPALHYYLERTRPDFMQFNEIS
CCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHCC
AEETCTLIPQMYGGLTSAAELKNIADVIEAYGIPGVSMTDSHRLRLSGIRPNDLASIRKA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHH
LNMPVFSPRHRWTIQIRACTCGQHRSFQELASRIERDTDTLAVSAYVSVSLSCENNCTDA
HCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHH
YIQDIGAIQTQTGWEIYIGGFRGKQARAGSLFCVTDNEDSTAAMMKGLIQYYRETAHYNE
HHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
AIYQWVDRLGLVHIREMLFEEDMRTQLLKNLRSDISLLQNLSAGKTPYERMKLFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: ammonium hydroxide; NAD+; NADP+; H2O

Specific reaction: ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H + 3 H+

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]