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The map label for this gene is nasD [H]

Identifier: 154684820

GI number: 154684820

Start: 325635

End: 328052

Strand: Reverse

Name: nasD [H]

Synonym: RBAM_003510

Alternate gene names: 154684820

Gene position: 328052-325635 (Counterclockwise)

Preceding gene: 154684821

Following gene: 154684819

Centisome position: 8.37

GC content: 49.83

Gene sequence:

>2418_bases
ATGGGAAAAAAACAGCTAGTCCTTGTTGGAAATGGAATGGCCGGGGTAAGAGCGATTGAAGAAATACTGCGTGTTTCAAA
AGACGAATTCGAGATTACAATATTCGGGGCCGAACCTCATCCCAACTATAACCGAATCCTTCTGTCAAAAGTGCTGCAAG
GCGATACCGACATTCAAGACATTACGTTAAACGATTGGAACTGGTACAAAGAAAACAACATTCAGCTGTATACCGGTGAA
ACAGTCATCAAAGTCGACACGGAAAACAAAACCGTCATCACCGATGCAGACAGAATCCAGCCTTACGATGAACTCATCCT
GGCGACAGGCTCTGTCCCTTTTATCCTTCCGATTCCGGGCGCAGACAAACAAGGCGTCACGGCTTTCCGGGATATAAAAG
ATACAGACACGATGCTCGCCGCGTCAAAACAATATAAAAAAGCAGCCGTCATCGGCGGAGGACTGCTTGGTTTGGAAGCC
GCTCGCGGACTGCTGAATCTCGGAATGGACGTGTCTGTCATCCACCTCGCCCCATTCCTGATGGAGCGCCAGCTTGACGC
AGCGGCGGGACGCCTGCTGCAAAACGAATTAGAAAAACAAGGCATGACCTTTTTCCTTGAAAAGCAAACCGAAGAAATTC
TCGGTACTGACCGTGTCGAAGGGGTACGCTTCAAAGACGGCACAAGCATCGAAGCTGACCTGGTCGTCATGGCGGTCGGC
ATCCGCCCGAATACACAGCTTGGCACAGAAAGCGGCATTCCGGTAAACCGCGGAATCATTGTCAACGATTATATGCAGAC
TGACATTCCGCACATTTACGCGGTCGGTGAATGTGCCGAGCACCGCGGAATCGCATACGGTCTTGTGGCGCCGCTCTATG
AACAGGCAAAAGTGCTGGCCAAACATCTTTGCGGCATTGAAACAAAACCGTATGAAGGCTCCGTTCTCTCCACTCAATTA
AAGGTATCAGGCGTCGAAGTCTTCTCTGCCGGTGACTTTAACGAATCAGAGGACAAAAAAGCGATTAAAGTGTTCGATGA
GCAGGACGGCATTTATAAAAAAATCGTCCTGAGGGGCAATCAAATTGTCGGCGCTGTTTTGTTCGGAGACAGCAGCGAGG
GCAGCCGCCTGTTTTCAATGATTCAAAAAGAAGCCGATATCTCTGAAACCTCCAAAATATCAATCTTACAGCCTCTTGAT
CAGGAAGCAGGCGCAAGCATGACCGCGGCGATGAGCGACGATGAAATCATCTGCGGATGCAACGGCGTATCAAAAGGCGC
GATTATTCAAGCCATTCAGGAAAAAGGCTGCACATCTACGGACGAAATCAAAGCATGCACCGGGGCATCCCGTTCCTGCG
GAGGCTGTAAACCGCTCGTGGAAGACATTCTTCAATATACACTGGGCTCAGAATTTGACGCGTCCGCGCAAAAAGAAGCG
ATTTGCGGCTGCACATCACTGTCGAGAGATGAAGTCGTAGAAGAAATCAAAGCCAAAGGGCTGTCCCATACAAGAGAAAT
CATGAACGTGCTCGGCTGGAAAACGCCGGAGGGCTGCTCCAAATGCCGTCCTGCCCTCAACTACTACTTAGGCATGATTA
ATCCGGCTACATACGAGGATGACAGAACATCCCGTTTTGTGAACGAACGGATGCACGCCAATATTCAAAAAGACGGAACA
TACTCAGTCGTTCCCCGGATGTACGGCGGCGTGACCAACTCTCAGGATCTCCGCAAGATCGCCGATGTCGTCGACAAATA
TGAGATCCCGCTCGTTAAAATGACAGGCGGCCAGCGAATTGATCTCATCGGCGTCAAAAAAGAAGATCTGCCGAAAGTAT
GGGCCGAGCTTGATATGCCGTCAGGCTACGCGTACGGAAAAACACTCCGCACGGTAAAAACGTGCGTAGGCGAACAATTC
TGCCGCTTCGGCACGCAAGACTCCATGGCACTGGGCATTGCGCTTGAGAAAAAATTCGAAGGGCTCAATACGCCTCATAA
GGTCAAAATGGCCGTGTCCGCCTGCCCGCGGAACTGTGCGGAATCAGGCATTAAGGACCTTGGCGTTGTCGGCATCGACG
GAGGCTGGGAGCTTTATGTCGGCGGAAACGGCGGAACCCATTTGCGCGCCGGTGATTTGCTGATGAAAGTAAAAACGAAT
GAAGAAGTCTTAGAATATGCGGGGGCTTACCTTCAGTACTACAGAGAAACGGCAAACTATCTGGAGCGGACATCCGCATG
GCTTGAGCGCGTCGGTTTATCTCATGTCCAATCCGTATTAAACGATCCGGAAAAAAGGCAGGAGCTCAACGACAGAATGA
ATGAGACACTCTCTGTCCACAAAGATCCGTGGCAATACTTCCTTAAAGATAAAAAAGCATCTAAAGATCTATTCGAAAAT
GTCGTCACGACTTCATAA

Upstream 100 bases:

>100_bases
ATTTTTTAGTATTTTTTGTTACATTTCATAACATGCACCTTAAATATTTGTTTTTGCTGTGCTACGCTAAAGAAAACATA
GATAAGGAGCGGATCATCTG

Downstream 100 bases:

>100_bases
AACAGTAAGGGGGAACGGACATGGTGAATAAAGATATGACAAAGGTTTGCATAGGCAAAGTAAACGAATTACCCGAGGAG
TTAGGAAAAACCGTATATAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MGKKQLVLVGNGMAGVRAIEEILRVSKDEFEITIFGAEPHPNYNRILLSKVLQGDTDIQDITLNDWNWYKENNIQLYTGE
TVIKVDTENKTVITDADRIQPYDELILATGSVPFILPIPGADKQGVTAFRDIKDTDTMLAASKQYKKAAVIGGGLLGLEA
ARGLLNLGMDVSVIHLAPFLMERQLDAAAGRLLQNELEKQGMTFFLEKQTEEILGTDRVEGVRFKDGTSIEADLVVMAVG
IRPNTQLGTESGIPVNRGIIVNDYMQTDIPHIYAVGECAEHRGIAYGLVAPLYEQAKVLAKHLCGIETKPYEGSVLSTQL
KVSGVEVFSAGDFNESEDKKAIKVFDEQDGIYKKIVLRGNQIVGAVLFGDSSEGSRLFSMIQKEADISETSKISILQPLD
QEAGASMTAAMSDDEIICGCNGVSKGAIIQAIQEKGCTSTDEIKACTGASRSCGGCKPLVEDILQYTLGSEFDASAQKEA
ICGCTSLSRDEVVEEIKAKGLSHTREIMNVLGWKTPEGCSKCRPALNYYLGMINPATYEDDRTSRFVNERMHANIQKDGT
YSVVPRMYGGVTNSQDLRKIADVVDKYEIPLVKMTGGQRIDLIGVKKEDLPKVWAELDMPSGYAYGKTLRTVKTCVGEQF
CRFGTQDSMALGIALEKKFEGLNTPHKVKMAVSACPRNCAESGIKDLGVVGIDGGWELYVGGNGGTHLRAGDLLMKVKTN
EEVLEYAGAYLQYYRETANYLERTSAWLERVGLSHVQSVLNDPEKRQELNDRMNETLSVHKDPWQYFLKDKKASKDLFEN
VVTTS

Sequences:

>Translated_805_residues
MGKKQLVLVGNGMAGVRAIEEILRVSKDEFEITIFGAEPHPNYNRILLSKVLQGDTDIQDITLNDWNWYKENNIQLYTGE
TVIKVDTENKTVITDADRIQPYDELILATGSVPFILPIPGADKQGVTAFRDIKDTDTMLAASKQYKKAAVIGGGLLGLEA
ARGLLNLGMDVSVIHLAPFLMERQLDAAAGRLLQNELEKQGMTFFLEKQTEEILGTDRVEGVRFKDGTSIEADLVVMAVG
IRPNTQLGTESGIPVNRGIIVNDYMQTDIPHIYAVGECAEHRGIAYGLVAPLYEQAKVLAKHLCGIETKPYEGSVLSTQL
KVSGVEVFSAGDFNESEDKKAIKVFDEQDGIYKKIVLRGNQIVGAVLFGDSSEGSRLFSMIQKEADISETSKISILQPLD
QEAGASMTAAMSDDEIICGCNGVSKGAIIQAIQEKGCTSTDEIKACTGASRSCGGCKPLVEDILQYTLGSEFDASAQKEA
ICGCTSLSRDEVVEEIKAKGLSHTREIMNVLGWKTPEGCSKCRPALNYYLGMINPATYEDDRTSRFVNERMHANIQKDGT
YSVVPRMYGGVTNSQDLRKIADVVDKYEIPLVKMTGGQRIDLIGVKKEDLPKVWAELDMPSGYAYGKTLRTVKTCVGEQF
CRFGTQDSMALGIALEKKFEGLNTPHKVKMAVSACPRNCAESGIKDLGVVGIDGGWELYVGGNGGTHLRAGDLLMKVKTN
EEVLEYAGAYLQYYRETANYLERTSAWLERVGLSHVQSVLNDPEKRQELNDRMNETLSVHKDPWQYFLKDKKASKDLFEN
VVTTS
>Mature_804_residues
GKKQLVLVGNGMAGVRAIEEILRVSKDEFEITIFGAEPHPNYNRILLSKVLQGDTDIQDITLNDWNWYKENNIQLYTGET
VIKVDTENKTVITDADRIQPYDELILATGSVPFILPIPGADKQGVTAFRDIKDTDTMLAASKQYKKAAVIGGGLLGLEAA
RGLLNLGMDVSVIHLAPFLMERQLDAAAGRLLQNELEKQGMTFFLEKQTEEILGTDRVEGVRFKDGTSIEADLVVMAVGI
RPNTQLGTESGIPVNRGIIVNDYMQTDIPHIYAVGECAEHRGIAYGLVAPLYEQAKVLAKHLCGIETKPYEGSVLSTQLK
VSGVEVFSAGDFNESEDKKAIKVFDEQDGIYKKIVLRGNQIVGAVLFGDSSEGSRLFSMIQKEADISETSKISILQPLDQ
EAGASMTAAMSDDEIICGCNGVSKGAIIQAIQEKGCTSTDEIKACTGASRSCGGCKPLVEDILQYTLGSEFDASAQKEAI
CGCTSLSRDEVVEEIKAKGLSHTREIMNVLGWKTPEGCSKCRPALNYYLGMINPATYEDDRTSRFVNERMHANIQKDGTY
SVVPRMYGGVTNSQDLRKIADVVDKYEIPLVKMTGGQRIDLIGVKKEDLPKVWAELDMPSGYAYGKTLRTVKTCVGEQFC
RFGTQDSMALGIALEKKFEGLNTPHKVKMAVSACPRNCAESGIKDLGVVGIDGGWELYVGGNGGTHLRAGDLLMKVKTNE
EVLEYAGAYLQYYRETANYLERTSAWLERVGLSHVQSVLNDPEKRQELNDRMNETLSVHKDPWQYFLKDKKASKDLFENV
VTTS

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=275, Percent_Identity=29.4545454545455, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI21389617, Length=275, Percent_Identity=29.4545454545455, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI226437568, Length=275, Percent_Identity=29.4545454545455, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI22202629, Length=267, Percent_Identity=25.4681647940075, Blast_Score=68, Evalue=5e-11,
Organism=Homo sapiens, GI4757732, Length=267, Percent_Identity=25.4681647940075, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI91199540, Length=248, Percent_Identity=29.4354838709677, Blast_Score=67, Evalue=8e-11,
Organism=Escherichia coli, GI1789765, Length=795, Percent_Identity=36.2264150943396, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1789065, Length=367, Percent_Identity=27.5204359673025, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI1788892, Length=284, Percent_Identity=27.4647887323944, Blast_Score=109, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=412, Percent_Identity=24.5145631067961, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI281359715, Length=324, Percent_Identity=27.4691358024691, Blast_Score=126, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281359713, Length=324, Percent_Identity=27.4691358024691, Blast_Score=126, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24639250, Length=324, Percent_Identity=27.4691358024691, Blast_Score=126, Evalue=7e-29,
Organism=Drosophila melanogaster, GI18543267, Length=324, Percent_Identity=27.4691358024691, Blast_Score=126, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24639252, Length=324, Percent_Identity=27.4691358024691, Blast_Score=126, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24639257, Length=324, Percent_Identity=27.4691358024691, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24585130, Length=334, Percent_Identity=23.6526946107784, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88477; Mature: 88346

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKQLVLVGNGMAGVRAIEEILRVSKDEFEITIFGAEPHPNYNRILLSKVLQGDTDIQD
CCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCE
ITLNDWNWYKENNIQLYTGETVIKVDTENKTVITDADRIQPYDELILATGSVPFILPIPG
EEECCCCEEECCCEEEEECCEEEEEECCCCEEEECHHHCCCHHHHEEECCCCCEEEECCC
ADKQGVTAFRDIKDTDTMLAASKQYKKAAVIGGGLLGLEAARGLLNLGMDVSVIHLAPFL
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCCEEHHHHHHHH
MERQLDAAAGRLLQNELEKQGMTFFLEKQTEEILGTDRVEGVRFKDGTSIEADLVVMAVG
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCEEECCCCCCCEEEEEEEEC
IRPNTQLGTESGIPVNRGIIVNDYMQTDIPHIYAVGECAEHRGIAYGLVAPLYEQAKVLA
CCCCCCCCCCCCCCCCCCEEEECHHHCCCCEEEEECHHHHHCCCCHHHHHHHHHHHHHHH
KHLCGIETKPYEGSVLSTQLKVSGVEVFSAGDFNESEDKKAIKVFDEQDGIYKKIVLRGN
HHHCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCCEEEECCCCCCEEHHEEECC
QIVGAVLFGDSSEGSRLFSMIQKEADISETSKISILQPLDQEAGASMTAAMSDDEIICGC
EEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHCCCCEEEECCCCCEEEEC
NGVSKGAIIQAIQEKGCTSTDEIKACTGASRSCGGCKPLVEDILQYTLGSEFDASAQKEA
CCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
ICGCTSLSRDEVVEEIKAKGLSHTREIMNVLGWKTPEGCSKCRPALNYYLGMINPATYED
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
DRTSRFVNERMHANIQKDGTYSVVPRMYGGVTNSQDLRKIADVVDKYEIPLVKMTGGQRI
CHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEE
DLIGVKKEDLPKVWAELDMPSGYAYGKTLRTVKTCVGEQFCRFGTQDSMALGIALEKKFE
EEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHC
GLNTPHKVKMAVSACPRNCAESGIKDLGVVGIDGGWELYVGGNGGTHLRAGDLLMKVKTN
CCCCCHHHHHHHHHCCHHHHHCCHHHCCEEEECCCEEEEECCCCCCEEEECCEEEEEECC
EEVLEYAGAYLQYYRETANYLERTSAWLERVGLSHVQSVLNDPEKRQELNDRMNETLSVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHCCC
KDPWQYFLKDKKASKDLFENVVTTS
CCHHHHHHHHCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKKQLVLVGNGMAGVRAIEEILRVSKDEFEITIFGAEPHPNYNRILLSKVLQGDTDIQD
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCE
ITLNDWNWYKENNIQLYTGETVIKVDTENKTVITDADRIQPYDELILATGSVPFILPIPG
EEECCCCEEECCCEEEEECCEEEEEECCCCEEEECHHHCCCHHHHEEECCCCCEEEECCC
ADKQGVTAFRDIKDTDTMLAASKQYKKAAVIGGGLLGLEAARGLLNLGMDVSVIHLAPFL
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCCEEHHHHHHHH
MERQLDAAAGRLLQNELEKQGMTFFLEKQTEEILGTDRVEGVRFKDGTSIEADLVVMAVG
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCEEECCCCCCCEEEEEEEEC
IRPNTQLGTESGIPVNRGIIVNDYMQTDIPHIYAVGECAEHRGIAYGLVAPLYEQAKVLA
CCCCCCCCCCCCCCCCCCEEEECHHHCCCCEEEEECHHHHHCCCCHHHHHHHHHHHHHHH
KHLCGIETKPYEGSVLSTQLKVSGVEVFSAGDFNESEDKKAIKVFDEQDGIYKKIVLRGN
HHHCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCCEEEECCCCCCEEHHEEECC
QIVGAVLFGDSSEGSRLFSMIQKEADISETSKISILQPLDQEAGASMTAAMSDDEIICGC
EEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHCCCCEEEECCCCCEEEEC
NGVSKGAIIQAIQEKGCTSTDEIKACTGASRSCGGCKPLVEDILQYTLGSEFDASAQKEA
CCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
ICGCTSLSRDEVVEEIKAKGLSHTREIMNVLGWKTPEGCSKCRPALNYYLGMINPATYED
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
DRTSRFVNERMHANIQKDGTYSVVPRMYGGVTNSQDLRKIADVVDKYEIPLVKMTGGQRI
CHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEE
DLIGVKKEDLPKVWAELDMPSGYAYGKTLRTVKTCVGEQFCRFGTQDSMALGIALEKKFE
EEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHC
GLNTPHKVKMAVSACPRNCAESGIKDLGVVGIDGGWELYVGGNGGTHLRAGDLLMKVKTN
CCCCCHHHHHHHHHCCHHHHHCCHHHCCEEEECCCEEEEECCCCCCEEEECCEEEEEECC
EEVLEYAGAYLQYYRETANYLERTSAWLERVGLSHVQSVLNDPEKRQELNDRMNETLSVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHCCC
KDPWQYFLKDKKASKDLFENVVTTS
CCHHHHHHHHCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]