BacMap

Definition	Bacillus amyloliquefaciens FZB42, complete genome.
Accession	NC_009725
Length	3,918,589

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The map label for this gene is kdgR1 [H]

Identifier: 154684712

GI number: 154684712

Start: 215385

End: 216230

Strand: Reverse

Name: kdgR1 [H]

Synonym: RBAM_002430

Alternate gene names: 154684712

Gene position: 216230-215385 (Counterclockwise)

Preceding gene: 154684713

Following gene: 154684704

Centisome position: 5.52

GC content: 50.95

Gene sequence:

>846_bases
ATGATGACTGGAGCAATTCCGCAAAAGAAACTGCTGGATGGGACAAGCATTCCCGCGATCGGCTTCGGTACATACGCTCT
TAACGGAAACAACGGCGTTCATGCCATTACCAGCGCCATTGATATCGGCTACCGTCTGATTGACACCGCTTATAACTATG
AAAATGAAGGGACGGTCGGAGAAGCCATCAAGAGAAGCTCTGTTCCGAGAGAACAGCTATACGTGACGTCCAAGCTGCCC
GGACGCTACCATGACTATGACAAAGCTGTACAAACCATACAAGAATCTTTATACCGCGCCGGTCTGGACTACTATGACCT
GTATCTCGTCCATTGGCCGAACCCGAAACAAGACCGCTACACGGAAGCATGGCAGGCACTGATTGATGCGAAAAAATGGG
GTCTGATCCGATCCATCGGCGTCTGCAATTTCCTGCCTGAGCATATCGAACGGCTCGAACGTGAAACCGGCGTAAAGCCA
GGCATCAACCAAATTGAACTGCATCCCGCTTTTAATCAGCAGCAGCAGCGCCAATGGCACGAAGAACAGGGCATCGTGAC
AGAATCATGGAGTCCGTTATCCCGCGCCGGCGAAGTATTGAACGATCAAAAACTCGGACGGATTGCGGACAAATACGGCA
AAACCATTTCCCAAGTCATTTTACGCTGGCATATCCAGCTGGGCGCCATCCCGATTCCAAAATCAGCATCGCCCGAGCGC
CAGCTTGAAAACATCTCCGTTTTTGATTTCTCTCTTGATGAAAACGACATGAGCATTATCGACTCGCTTACCCGCCCCGA
CGGACGCATAAACGGCCAAGACCCGGCGGAGTATGAGGAGTTTTAA

Upstream 100 bases:

>100_bases
TCTCCGTCAGCTTTTTTCATGTGCGGATCATCTCATTTCCTTCCATTATTATGTTTCATTTGTAAGAATAATGGTAAAGT
AGAAAACAAGGAGGGATTTA

Downstream 100 bases:

>100_bases
GTAAACGCATAAAGAAAAGCTGACGGCATCATTTGTCAGCTTTTTTCTCATTTTATATACGCACACCCAGCTCCCGTTTC
CATTCAGAAAGCCAATCTGT

Product: KdgR1

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVGEAIKRSSVPREQLYVTSKLP
GRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRYTEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKP
GINQIELHPAFNQQQQRQWHEEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER
QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF

Sequences:

>Translated_281_residues
MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVGEAIKRSSVPREQLYVTSKLP
GRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRYTEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKP
GINQIELHPAFNQQQQRQWHEEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER
QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF
>Mature_281_residues
MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVGEAIKRSSVPREQLYVTSKLP
GRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRYTEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKP
GINQIELHPAFNQQQQRQWHEEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER
QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=284, Percent_Identity=35.9154929577465, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI291291012, Length=264, Percent_Identity=35.9848484848485, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI24497577, Length=287, Percent_Identity=32.404181184669, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI5174391, Length=287, Percent_Identity=32.404181184669, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI5174695, Length=302, Percent_Identity=33.7748344370861, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=34.7222222222222, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=32.2033898305085, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI5453543, Length=293, Percent_Identity=33.7883959044369, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI310109920, Length=293, Percent_Identity=33.7883959044369, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=34.9090909090909, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=33.1058020477816, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI45446745, Length=293, Percent_Identity=33.4470989761092, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI4503285, Length=293, Percent_Identity=33.4470989761092, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI24497585, Length=293, Percent_Identity=33.4470989761092, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI300116273, Length=284, Percent_Identity=33.0985915492958, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI300116271, Length=285, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI310109924, Length=148, Percent_Identity=33.1081081081081, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI310109926, Length=124, Percent_Identity=39.5161290322581, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI207028673, Length=124, Percent_Identity=38.7096774193548, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87082198, Length=270, Percent_Identity=46.6666666666667, Blast_Score=248, Evalue=2e-67,
Organism=Escherichia coli, GI1786400, Length=261, Percent_Identity=37.1647509578544, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1788081, Length=276, Percent_Identity=28.6231884057971, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI48994888, Length=239, Percent_Identity=25.9414225941423, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537075, Length=288, Percent_Identity=38.8888888888889, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=38.5416666666667, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71998625, Length=272, Percent_Identity=34.9264705882353, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=33.4558823529412, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=32, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17537079, Length=291, Percent_Identity=31.9587628865979, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17566692, Length=296, Percent_Identity=29.7297297297297, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=33.9483394833948, Blast_Score=143, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17550248, Length=304, Percent_Identity=32.5657894736842, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=252, Percent_Identity=32.9365079365079, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=29.0566037735849, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320576, Length=288, Percent_Identity=38.5416666666667, Blast_Score=177, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324694, Length=285, Percent_Identity=34.3859649122807, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6322556, Length=261, Percent_Identity=31.8007662835249, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6319625, Length=283, Percent_Identity=33.9222614840989, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6321896, Length=312, Percent_Identity=31.0897435897436, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320079, Length=278, Percent_Identity=29.8561151079137, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24657054, Length=292, Percent_Identity=39.3835616438356, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=38.1443298969072, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI20129731, Length=282, Percent_Identity=35.4609929078014, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=35.7894736842105, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI45553081, Length=280, Percent_Identity=32.8571428571429, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24644950, Length=290, Percent_Identity=32.4137931034483, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=31.0580204778157, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI221378297, Length=310, Percent_Identity=30.3225806451613, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24662785, Length=277, Percent_Identity=35.3790613718412, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24662781, Length=279, Percent_Identity=34.7670250896057, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281366140, Length=245, Percent_Identity=32.6530612244898, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 31903; Mature: 31903

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVG
CCCCCCCHHHHCCCCCCCEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EAIKRSSVPREQLYVTSKLPGRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRY
HHHHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHH
TEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKPGINQIELHPAFNQQQQRQWH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
EEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF
HHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MMTGAIPQKKLLDGTSIPAIGFGTYALNGNNGVHAITSAIDIGYRLIDTAYNYENEGTVG
CCCCCCCHHHHCCCCCCCEEECEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EAIKRSSVPREQLYVTSKLPGRYHDYDKAVQTIQESLYRAGLDYYDLYLVHWPNPKQDRY
HHHHHHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHH
TEAWQALIDAKKWGLIRSIGVCNFLPEHIERLERETGVKPGINQIELHPAFNQQQQRQWH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHH
EEQGIVTESWSPLSRAGEVLNDQKLGRIADKYGKTISQVILRWHIQLGAIPIPKSASPER
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
QLENISVFDFSLDENDMSIIDSLTRPDGRINGQDPAEYEEF
HHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA