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The map label for this gene is yaaI [H]

Identifier: 154684536

GI number: 154684536

Start: 26259

End: 26810

Strand: Reverse

Name: yaaI [H]

Synonym: RBAM_000220

Alternate gene names: 154684536

Gene position: 26810-26259 (Counterclockwise)

Preceding gene: 154684556

Following gene: 154684535

Centisome position: 0.68

GC content: 42.21

Gene sequence:

>552_bases
TTGTCTGAACCTAACAACACAGCCCTTCTGATTGTCGATATGATTAACAATTTTCAATTTGATATGGGCGAACGTCTTGC
TGAAAAAACCAAACAAATTGTCCCCCGTATTTTATCATTAAAGAAGCATGCAAAACAAAACAACTGGCCGATTATTTATA
TTAATGATCATTACGGGCTCTGGAAGGCTGATATTGATGCCATCAGGGAGGAATGCAGCAACGACAGAAGCGCCCCCATT
ATAAAAGATATTTCGCCTCAGCCGGATGATTATTTTCTCATTAAACCGAAGCATTCCGCTTTTTACGAAACGGCGCTTCA
CACCCTTCTGACCGAATTACAAGTGAACCGATTAGTCATCACGGGGATTGCCGGGAACATTTGTGTGCTGTTCACGGCCA
ACGACGCCTATATGAGAGAATACAGCATTGCCATTCCGAAAGACTGCACCGCTTCCAATAACGACGAAGACAATGATTTC
GCTTTGACCATGATGGAGAATGTTCTCTTCGCAGAAATAACCACGGAAAAACAAATTACCGCCGAAAAATAA

Upstream 100 bases:

>100_bases
ATAATATTCATCATTCATAGGTTCGAGCTCTCTTCTTAAGTTTTTTCATGTCTCATAAAGGTTAAAATAGGAATACCCCA
GCAGAAAGGAGTTGCCGGTA

Downstream 100 bases:

>100_bases
ACATGATCAGCCTCCTTTTTTCATATATTTGATAGCGGATATGAAAGGAGGCGTTTTTCATTCAGATCTATATAGTGAAA
CGGGGCGACACGCTTTCCGC

Product: YaaI

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MSEPNNTALLIVDMINNFQFDMGERLAEKTKQIVPRILSLKKHAKQNNWPIIYINDHYGLWKADIDAIREECSNDRSAPI
IKDISPQPDDYFLIKPKHSAFYETALHTLLTELQVNRLVITGIAGNICVLFTANDAYMREYSIAIPKDCTASNNDEDNDF
ALTMMENVLFAEITTEKQITAEK

Sequences:

>Translated_183_residues
MSEPNNTALLIVDMINNFQFDMGERLAEKTKQIVPRILSLKKHAKQNNWPIIYINDHYGLWKADIDAIREECSNDRSAPI
IKDISPQPDDYFLIKPKHSAFYETALHTLLTELQVNRLVITGIAGNICVLFTANDAYMREYSIAIPKDCTASNNDEDNDF
ALTMMENVLFAEITTEKQITAEK
>Mature_182_residues
SEPNNTALLIVDMINNFQFDMGERLAEKTKQIVPRILSLKKHAKQNNWPIIYINDHYGLWKADIDAIREECSNDRSAPII
KDISPQPDDYFLIKPKHSAFYETALHTLLTELQVNRLVITGIAGNICVLFTANDAYMREYSIAIPKDCTASNNDEDNDFA
LTMMENVLFAEITTEKQITAEK

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=193, Percent_Identity=26.9430051813472, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 20898; Mature: 20767

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPNNTALLIVDMINNFQFDMGERLAEKTKQIVPRILSLKKHAKQNNWPIIYINDHYGL
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
WKADIDAIREECSNDRSAPIIKDISPQPDDYFLIKPKHSAFYETALHTLLTELQVNRLVI
CHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCEEEE
TGIAGNICVLFTANDAYMREYSIAIPKDCTASNNDEDNDFALTMMENVLFAEITTEKQIT
EECCCCEEEEEECCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCC
AEK
CCC
>Mature Secondary Structure 
SEPNNTALLIVDMINNFQFDMGERLAEKTKQIVPRILSLKKHAKQNNWPIIYINDHYGL
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
WKADIDAIREECSNDRSAPIIKDISPQPDDYFLIKPKHSAFYETALHTLLTELQVNRLVI
CHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCEEEE
TGIAGNICVLFTANDAYMREYSIAIPKDCTASNNDEDNDFALTMMENVLFAEITTEKQIT
EECCCCEEEEEECCCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCC
AEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]