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The map label for this gene is nqo13 [H]

Identifier: 154248540

GI number: 154248540

Start: 5107139

End: 5108650

Strand: Reverse

Name: nqo13 [H]

Synonym: Xaut_4621

Alternate gene names: 154248540

Gene position: 5108650-5107139 (Counterclockwise)

Preceding gene: 154248541

Following gene: 154248539

Centisome position: 96.23

GC content: 63.36

Gene sequence:

>1512_bases
ATGACCGACTGGCCCATCCTCTCCGTCGTAACCTTCCTGCCGCTGGTCGGCGCGCTCTTCATCCTGGTGGTGCGGGGCGA
TGACGAGGTGACCGCACGCAACGCCCGCTGGGTGGCGCTGTGGACCACCCTCGTCACCTTCGTGGTGTCGCTGCTGCTGC
TGCCGGGCTTCGACTCCGCGGCGTCCGGCTTCCAGTTCGTGGAAAAGAAGGCGTGGCTCGGCGAACTCGCCGCCTACCAC
ATGGGCGTGGACGGCATCTCGCTTCCCTTCGTCATCCTCACCACGTTCCTCATGCCCTTCTGCATCCTCGCCTCCTGGGA
GAGCGTGCATAAGCGCGTGAAGGAATACATGATCGCGTTCCTGGTGCTCGAAACGCTGATGATCGGCACCTTCTGCGCGC
TGGACCTCGTCCTCTTCTATTTCTTCTTCGAGGGCGGCCTCATCCCGATGTTCCTCATCATCGGCATCTGGGGCGGTCAG
CGGCGCATCTACGCGGCCTTCAAGTTCTTCCTCTACACGCTGGCCGGTTCCGTGCTGATGATGCTCGCCATCATGGCCAT
GTACTGGCAGTCCGGCACCACCGACGTGCCGACGCTGCTGACCCACGGTTTCCCGTCCGGCATGCAGACTTGGCTGTGGC
TGGCCTTCTTCGCCTCCTTCGCGGTGAAGATGCCCATGTGGCCGGTGCACACCTGGCTGCCCGACGCCCACGTGGAGGCG
CCCACCGCCGGTTCGGTCATTCTGGCGGGCGTGCTGCTGAAGATGGGCGGATACGGCTTCCTGCGTTACTCGCTGCCCAT
GTTCCCGGAAGCGAGCCACTATTTCGCGCCGCTGGTGTTCACCCTCTCGGTGGTGGCCATCATCTACACGTCGCTGGTGG
CCCTCGTTCAGGAGGACATCAAGAAGCTGATCGCTTACTCGTCCATTGCCCACATGGGCTTCGTGACTATGGGCATCTTC
GCCATGAACGAGCAGGGCCTGCAGGGGGCCATGTTCCTCATGATCTCCCACGGCTTCGTCTCCGGCGCGCTGTTCCTGTG
CGTGGGCGTCATCTATGACCGGCTGCACACCCGCGAGATCGCGGCCTATGGCGGCCTCGTGCATCGCATGCCGCTCTACG
CCACGGTGCTGATGATCTTCACCATGGCAAACGTGGGCCTGCCGGGCACCTCGGGCTTCATCGGCGAGTTCCTGACCATG
CTCGCCGCCTTCGAGCGCAACACCTGGATCGCCTTCTTCGCGGCAACCGGCGTGATCCTTTCGGCGGGCTATGCCCTGTG
GCTCTACCTGCGCGTGATCTTCGGGCCGCTCGAGAAGGAGAAGCTGAAGACCATGATGGACCTCTCCCCCCGGGAGATCG
TGATCATGGTGCCTCTGGTGATCCTCACCATTCTCTTCGGCGTGTGGCCCGCGCCCATCCTCAACATGACATCCCATGCG
GTGAACGCGGTCGTCGCGCGTACCGATTCTGTGGCGGGTGCGGTGAAGACCGCCCTGCTGCTGAGCTTCTGA

Upstream 100 bases:

>100_bases
TGGTGCGGCTCCAGACCGGCTACCTCTACCACTACGCCTTCGTGATGCTCATCGGCGTCGCGGCTTTCATCACCTGGTTC
ATGTTCGCCGGAGTGTGACG

Downstream 100 bases:

>100_bases
TCCGAGGTCGCCTCAATGACGCAGCTCCTGCCTCCCATCGGCGCGGTCCTGCCCGAACTGGTGCTCGCCGTCTCGGCGAT
CGTGCTGATCCTGATCGGTG

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTDWPILSVVTFLPLVGALFILVVRGDDEVTARNARWVALWTTLVTFVVSLLLLPGFDSAASGFQFVEKKAWLGELAAYH
MGVDGISLPFVILTTFLMPFCILASWESVHKRVKEYMIAFLVLETLMIGTFCALDLVLFYFFFEGGLIPMFLIIGIWGGQ
RRIYAAFKFFLYTLAGSVLMMLAIMAMYWQSGTTDVPTLLTHGFPSGMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAGVLLKMGGYGFLRYSLPMFPEASHYFAPLVFTLSVVAIIYTSLVALVQEDIKKLIAYSSIAHMGFVTMGIF
AMNEQGLQGAMFLMISHGFVSGALFLCVGVIYDRLHTREIAAYGGLVHRMPLYATVLMIFTMANVGLPGTSGFIGEFLTM
LAAFERNTWIAFFAATGVILSAGYALWLYLRVIFGPLEKEKLKTMMDLSPREIVIMVPLVILTILFGVWPAPILNMTSHA
VNAVVARTDSVAGAVKTALLLSF

Sequences:

>Translated_503_residues
MTDWPILSVVTFLPLVGALFILVVRGDDEVTARNARWVALWTTLVTFVVSLLLLPGFDSAASGFQFVEKKAWLGELAAYH
MGVDGISLPFVILTTFLMPFCILASWESVHKRVKEYMIAFLVLETLMIGTFCALDLVLFYFFFEGGLIPMFLIIGIWGGQ
RRIYAAFKFFLYTLAGSVLMMLAIMAMYWQSGTTDVPTLLTHGFPSGMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAGVLLKMGGYGFLRYSLPMFPEASHYFAPLVFTLSVVAIIYTSLVALVQEDIKKLIAYSSIAHMGFVTMGIF
AMNEQGLQGAMFLMISHGFVSGALFLCVGVIYDRLHTREIAAYGGLVHRMPLYATVLMIFTMANVGLPGTSGFIGEFLTM
LAAFERNTWIAFFAATGVILSAGYALWLYLRVIFGPLEKEKLKTMMDLSPREIVIMVPLVILTILFGVWPAPILNMTSHA
VNAVVARTDSVAGAVKTALLLSF
>Mature_502_residues
TDWPILSVVTFLPLVGALFILVVRGDDEVTARNARWVALWTTLVTFVVSLLLLPGFDSAASGFQFVEKKAWLGELAAYHM
GVDGISLPFVILTTFLMPFCILASWESVHKRVKEYMIAFLVLETLMIGTFCALDLVLFYFFFEGGLIPMFLIIGIWGGQR
RIYAAFKFFLYTLAGSVLMMLAIMAMYWQSGTTDVPTLLTHGFPSGMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEAP
TAGSVILAGVLLKMGGYGFLRYSLPMFPEASHYFAPLVFTLSVVAIIYTSLVALVQEDIKKLIAYSSIAHMGFVTMGIFA
MNEQGLQGAMFLMISHGFVSGALFLCVGVIYDRLHTREIAAYGGLVHRMPLYATVLMIFTMANVGLPGTSGFIGEFLTML
AAFERNTWIAFFAATGVILSAGYALWLYLRVIFGPLEKEKLKTMMDLSPREIVIMVPLVILTILFGVWPAPILNMTSHAV
NAVVARTDSVAGAVKTALLLSF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=367, Percent_Identity=35.6948228882834, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI251831117, Length=352, Percent_Identity=25.8522727272727, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1788613, Length=476, Percent_Identity=37.6050420168067, Blast_Score=297, Evalue=9e-82,
Organism=Escherichia coli, GI1788827, Length=253, Percent_Identity=31.2252964426877, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788831, Length=294, Percent_Identity=27.891156462585, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788614, Length=488, Percent_Identity=26.6393442622951, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693160, Length=354, Percent_Identity=25.4237288135593, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788829, Length=415, Percent_Identity=26.2650602409639, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367154, Length=263, Percent_Identity=25.0950570342205, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55618; Mature: 55487

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDWPILSVVTFLPLVGALFILVVRGDDEVTARNARWVALWTTLVTFVVSLLLLPGFDSA
CCCHHHHHHHHHHHHHHHHEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCH
ASGFQFVEKKAWLGELAAYHMGVDGISLPFVILTTFLMPFCILASWESVHKRVKEYMIAF
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLETLMIGTFCALDLVLFYFFFEGGLIPMFLIIGIWGGQRRIYAAFKFFLYTLAGSVLM
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
MLAIMAMYWQSGTTDVPTLLTHGFPSGMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
HHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC
PTAGSVILAGVLLKMGGYGFLRYSLPMFPEASHYFAPLVFTLSVVAIIYTSLVALVQEDI
CCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLIAYSSIAHMGFVTMGIFAMNEQGLQGAMFLMISHGFVSGALFLCVGVIYDRLHTREI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAYGGLVHRMPLYATVLMIFTMANVGLPGTSGFIGEFLTMLAAFERNTWIAFFAATGVIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
SAGYALWLYLRVIFGPLEKEKLKTMMDLSPREIVIMVPLVILTILFGVWPAPILNMTSHA
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VNAVVARTDSVAGAVKTALLLSF
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]