Click here to switch to the map view.

The map label for this gene is nuoN [H]

Identifier: 154248539

GI number: 154248539

Start: 5105693

End: 5107123

Strand: Reverse

Name: nuoN [H]

Synonym: Xaut_4620

Alternate gene names: 154248539

Gene position: 5107123-5105693 (Counterclockwise)

Preceding gene: 154248540

Following gene: 154248538

Centisome position: 96.2

GC content: 65.41

Gene sequence:

>1431_bases
ATGACGCAGCTCCTGCCTCCCATCGGCGCGGTCCTGCCCGAACTGGTGCTCGCCGTCTCGGCGATCGTGCTGATCCTGAT
CGGTGCGTTCCGCGGAGAGGGGTCGGCGAATCTCGTCACCGGCCTCGCCATCGCCGTGCTCGCGGCGGTGGGTGTGCTGG
TGCTGCTCCAGCCGGCGGCGGAAGTCACCGCCTTCAACGGCTCCCTCGTGGTGGACGCCTACAGCCGCTTCATGAAGCTC
CTCGCCGTGCTCGGCGCCGTGGTCTCGCTGATCATGGCGGTGGACTGGCAGGCGCGGGAGAAGCAGGCGAAGTTCGAGTA
CGCCGTGCTCATCGTCATCGCCACGCTTGGCATGTGCATGCTGGTGTCGGCGGGTGACCTGATCGCGCTCTATCTCGGCC
TCGAGCTGATGAGCCTGTCGCTCTATGTGGTGGCCTCCATCAACCGCGACTCCGTCCGCTCGACCGAGGCTGGCCTGAAG
TATTTCGTTCTCGGTGCCTTGTCTTCGGGCATGCTGCTCTACGGCGCTTCGTTCATCTACGGCTTCACCGGTTCGGTGAA
CTTCCTGCAGATCGCCGCCGTGGCAAAGGAGCCCAGCCCGGGCCTCATCTTCGGCATCGTGTTCCTGCTGGCGGGCCTAT
GCTTCAAGGTGTCGGCGGTGCCGTTCCACATGTGGACGCCGGACGTCTATGAGGGCGCGCCGACCCCGGTCACGGCCTTC
TTCGCCTCCGCGCCCAAGGTGGCGGCCATGGCCATCTTCGTGCGCGTCGTCATCGAGGCATTCCCGCACGTGACCCACCA
GTGGCAGCAGATCGTCGCCTTCGTCTCGCTCGCCTCCATGGTGCTCGGCGCCTTCGCCGCCATCGGCCAGCGCAATATCA
AGCGCCTGCTGGCCTATTCCTCCATCGGCCACATGGGCTTCGCGCTGGTGGGTCTTGCGGCCGGCACGGCGGAAGGCGTA
CGCGGCGTGCTGCTGTACATGACCATCTATGTGGTGATGACGCTCGGCACCTTCGCCTGCGTGCTGACCATGCGCCGCAA
GGGTCAGGCCGTGGAAACCATCGAGGACCTCTCCGGCCTCGCCCGCCGCAACCCGCTGATGGCGCTGGCGCTGGGCGCGC
TCATGTTCTCCCTCGCCGGCATTCCGCCGCTGGCCGGCTTCCTTGCCAAATACTATGTGTTCGTGGCGGCGATCCAGGCC
GGGCTTTACGCCCTCGCGGTGGTGGGCGTGCTTGCCTCGGTGGTAGGCGCGTTCTATTACCTGCGTGTGGTCAAGATCAT
GTATTTCGACGAGCCGGTCGAGGCCTTCGATCCCATGCCGACGGAACTGAAGGCGGTGCTGGCGGTGAGTGGGCTTTTCA
CCATCTTCTATTTCGTCTATCCGGCTCCTCTTATCGAGGCGGCGGGTGTCGCGGCCCGTTCGCTCTTCTGA

Upstream 100 bases:

>100_bases
GACATCCCATGCGGTGAACGCGGTCGTCGCGCGTACCGATTCTGTGGCGGGTGCGGTGAAGACCGCCCTGCTGCTGAGCT
TCTGATCCGAGGTCGCCTCA

Downstream 100 bases:

>100_bases
TCGATGGCTCATTACGAATTCCAGGGTTCCACGGTTCCCATCGTCCGCTTCACCCAGATCGGCTCCACCAACGCCGAGGG
GATGGCGCGCCTTGGCCGGA

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTQLLPPIGAVLPELVLAVSAIVLILIGAFRGEGSANLVTGLAIAVLAAVGVLVLLQPAAEVTAFNGSLVVDAYSRFMKL
LAVLGAVVSLIMAVDWQAREKQAKFEYAVLIVIATLGMCMLVSAGDLIALYLGLELMSLSLYVVASINRDSVRSTEAGLK
YFVLGALSSGMLLYGASFIYGFTGSVNFLQIAAVAKEPSPGLIFGIVFLLAGLCFKVSAVPFHMWTPDVYEGAPTPVTAF
FASAPKVAAMAIFVRVVIEAFPHVTHQWQQIVAFVSLASMVLGAFAAIGQRNIKRLLAYSSIGHMGFALVGLAAGTAEGV
RGVLLYMTIYVVMTLGTFACVLTMRRKGQAVETIEDLSGLARRNPLMALALGALMFSLAGIPPLAGFLAKYYVFVAAIQA
GLYALAVVGVLASVVGAFYYLRVVKIMYFDEPVEAFDPMPTELKAVLAVSGLFTIFYFVYPAPLIEAAGVAARSLF

Sequences:

>Translated_476_residues
MTQLLPPIGAVLPELVLAVSAIVLILIGAFRGEGSANLVTGLAIAVLAAVGVLVLLQPAAEVTAFNGSLVVDAYSRFMKL
LAVLGAVVSLIMAVDWQAREKQAKFEYAVLIVIATLGMCMLVSAGDLIALYLGLELMSLSLYVVASINRDSVRSTEAGLK
YFVLGALSSGMLLYGASFIYGFTGSVNFLQIAAVAKEPSPGLIFGIVFLLAGLCFKVSAVPFHMWTPDVYEGAPTPVTAF
FASAPKVAAMAIFVRVVIEAFPHVTHQWQQIVAFVSLASMVLGAFAAIGQRNIKRLLAYSSIGHMGFALVGLAAGTAEGV
RGVLLYMTIYVVMTLGTFACVLTMRRKGQAVETIEDLSGLARRNPLMALALGALMFSLAGIPPLAGFLAKYYVFVAAIQA
GLYALAVVGVLASVVGAFYYLRVVKIMYFDEPVEAFDPMPTELKAVLAVSGLFTIFYFVYPAPLIEAAGVAARSLF
>Mature_475_residues
TQLLPPIGAVLPELVLAVSAIVLILIGAFRGEGSANLVTGLAIAVLAAVGVLVLLQPAAEVTAFNGSLVVDAYSRFMKLL
AVLGAVVSLIMAVDWQAREKQAKFEYAVLIVIATLGMCMLVSAGDLIALYLGLELMSLSLYVVASINRDSVRSTEAGLKY
FVLGALSSGMLLYGASFIYGFTGSVNFLQIAAVAKEPSPGLIFGIVFLLAGLCFKVSAVPFHMWTPDVYEGAPTPVTAFF
ASAPKVAAMAIFVRVVIEAFPHVTHQWQQIVAFVSLASMVLGAFAAIGQRNIKRLLAYSSIGHMGFALVGLAAGTAEGVR
GVLLYMTIYVVMTLGTFACVLTMRRKGQAVETIEDLSGLARRNPLMALALGALMFSLAGIPPLAGFLAKYYVFVAAIQAG
LYALAVVGVLASVVGAFYYLRVVKIMYFDEPVEAFDPMPTELKAVLAVSGLFTIFYFVYPAPLIEAAGVAARSLF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831108, Length=294, Percent_Identity=22.108843537415, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI145693160, Length=474, Percent_Identity=33.1223628691983, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1788614, Length=386, Percent_Identity=25.9067357512953, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788831, Length=298, Percent_Identity=27.1812080536913, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788827, Length=281, Percent_Identity=27.0462633451957, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788613, Length=386, Percent_Identity=23.3160621761658, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788829, Length=259, Percent_Identity=25.8687258687259, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50360; Mature: 50229

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLLPPIGAVLPELVLAVSAIVLILIGAFRGEGSANLVTGLAIAVLAAVGVLVLLQPAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
EVTAFNGSLVVDAYSRFMKLLAVLGAVVSLIMAVDWQAREKQAKFEYAVLIVIATLGMCM
HHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LVSAGDLIALYLGLELMSLSLYVVASINRDSVRSTEAGLKYFVLGALSSGMLLYGASFIY
HHCCCHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GFTGSVNFLQIAAVAKEPSPGLIFGIVFLLAGLCFKVSAVPFHMWTPDVYEGAPTPVTAF
HCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCHHCCCCCCCHHHH
FASAPKVAAMAIFVRVVIEAFPHVTHQWQQIVAFVSLASMVLGAFAAIGQRNIKRLLAYS
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIGHMGFALVGLAAGTAEGVRGVLLYMTIYVVMTLGTFACVLTMRRKGQAVETIEDLSGL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARRNPLMALALGALMFSLAGIPPLAGFLAKYYVFVAAIQAGLYALAVVGVLASVVGAFYY
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRVVKIMYFDEPVEAFDPMPTELKAVLAVSGLFTIFYFVYPAPLIEAAGVAARSLF
HHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC
>Mature Secondary Structure 
TQLLPPIGAVLPELVLAVSAIVLILIGAFRGEGSANLVTGLAIAVLAAVGVLVLLQPAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH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HHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA