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The map label for this gene is birA [H]

Identifier: 154248538

GI number: 154248538

Start: 5104922

End: 5105689

Strand: Reverse

Name: birA [H]

Synonym: Xaut_4619

Alternate gene names: 154248538

Gene position: 5105689-5104922 (Counterclockwise)

Preceding gene: 154248539

Following gene: 154248537

Centisome position: 96.17

GC content: 67.58

Gene sequence:

>768_bases
ATGGCTCATTACGAATTCCAGGGTTCCACGGTTCCCATCGTCCGCTTCACCCAGATCGGCTCCACCAACGCCGAGGGGAT
GGCGCGCCTTGGCCGGAGTGAGACGTTTCCGGTCTGGCTGGTGGCGGACATCCAGACCGCCGGCCGCGGCCGGCGGGGCC
GTCCCTGGACCTCCGAGCTCGGTAATCTGTTCGCCAGCCTCGTGCTGCCCAATCCGGCGCCGGTGGCAAACCTGGCGCAG
CTGTGCTTCGTGGCGGGTCTTGCCCTTCGCGACGCCGTGCTGGCCGCCGCACCGCAGATCCGTCCGGACGCGCTCAGGCT
CAAATGGCCCAACGACCTCCTGCTGGACGGCGGCAAGGTGGCCGGCATCCTCGTGGAAGGCGGCGCTGATCCCTCGGGCC
GGCCCGCGGCAGTGATCGGCTTCGGCGTCAACTGCCTGCACCATCCCGACGATCTGCCCTACCGTGCCACCAGCCTCGAA
GAATCGGGAGCGCCCACGGCACCAGACCGGCTGTTGGAAGCCCTCGACCTCGCCATGGCGGTGCGGCTCAACCAGTGGAA
TGCGGGCGCGGGTTTTCCCGCCATCCGGTCCGACTGGATGCGCTATGCGCTCGGCATGGGCGACCAGGTGAGCGTCAAGA
TCGGCGAGCGCGAGGTGGTGGGCCGTTTTGAGAACATCGATACCCGCGGAGCCATGATGCTGCGCCGTAAGGATGGGATC
GCGGAGATCATCACGGCGGGCGATGTCTCCTTGGGCAAGTCGGACTGA

Upstream 100 bases:

>100_bases
GGTGCTGGCGGTGAGTGGGCTTTTCACCATCTTCTATTTCGTCTATCCGGCTCCTCTTATCGAGGCGGCGGGTGTCGCGG
CCCGTTCGCTCTTCTGATCG

Downstream 100 bases:

>100_bases
GAGGCCGGATTGAGCCGCACAGGCGTCGTGCGGCCCCTTGCATTGCGGCCCGCAGCCCGCTTCATTGGGATCGATCCTGA
TTGCCCGCCCCTAGCCGGAT

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAHYEFQGSTVPIVRFTQIGSTNAEGMARLGRSETFPVWLVADIQTAGRGRRGRPWTSELGNLFASLVLPNPAPVANLAQ
LCFVAGLALRDAVLAAAPQIRPDALRLKWPNDLLLDGGKVAGILVEGGADPSGRPAAVIGFGVNCLHHPDDLPYRATSLE
ESGAPTAPDRLLEALDLAMAVRLNQWNAGAGFPAIRSDWMRYALGMGDQVSVKIGEREVVGRFENIDTRGAMMLRRKDGI
AEIITAGDVSLGKSD

Sequences:

>Translated_255_residues
MAHYEFQGSTVPIVRFTQIGSTNAEGMARLGRSETFPVWLVADIQTAGRGRRGRPWTSELGNLFASLVLPNPAPVANLAQ
LCFVAGLALRDAVLAAAPQIRPDALRLKWPNDLLLDGGKVAGILVEGGADPSGRPAAVIGFGVNCLHHPDDLPYRATSLE
ESGAPTAPDRLLEALDLAMAVRLNQWNAGAGFPAIRSDWMRYALGMGDQVSVKIGEREVVGRFENIDTRGAMMLRRKDGI
AEIITAGDVSLGKSD
>Mature_254_residues
AHYEFQGSTVPIVRFTQIGSTNAEGMARLGRSETFPVWLVADIQTAGRGRRGRPWTSELGNLFASLVLPNPAPVANLAQL
CFVAGLALRDAVLAAAPQIRPDALRLKWPNDLLLDGGKVAGILVEGGADPSGRPAAVIGFGVNCLHHPDDLPYRATSLEE
SGAPTAPDRLLEALDLAMAVRLNQWNAGAGFPAIRSDWMRYALGMGDQVSVKIGEREVVGRFENIDTRGAMMLRRKDGIA
EIITAGDVSLGKSD

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=239, Percent_Identity=30.9623430962343, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27252; Mature: 27120

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHYEFQGSTVPIVRFTQIGSTNAEGMARLGRSETFPVWLVADIQTAGRGRRGRPWTSEL
CCEEECCCCEEEEEEEEECCCCCHHHHHHCCCCCCCCEEEEEEEHHCCCCCCCCCHHHHH
GNLFASLVLPNPAPVANLAQLCFVAGLALRDAVLAAAPQIRPDALRLKWPNDLLLDGGKV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCEE
AGILVEGGADPSGRPAAVIGFGVNCLHHPDDLPYRATSLEESGAPTAPDRLLEALDLAMA
EEEEEECCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VRLNQWNAGAGFPAIRSDWMRYALGMGDQVSVKIGEREVVGRFENIDTRGAMMLRRKDGI
HEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCHHEEEECCCCC
AEIITAGDVSLGKSD
EEEEECCCCCCCCCC
>Mature Secondary Structure 
AHYEFQGSTVPIVRFTQIGSTNAEGMARLGRSETFPVWLVADIQTAGRGRRGRPWTSEL
CEEECCCCEEEEEEEEECCCCCHHHHHHCCCCCCCCEEEEEEEHHCCCCCCCCCHHHHH
GNLFASLVLPNPAPVANLAQLCFVAGLALRDAVLAAAPQIRPDALRLKWPNDLLLDGGKV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCEE
AGILVEGGADPSGRPAAVIGFGVNCLHHPDDLPYRATSLEESGAPTAPDRLLEALDLAMA
EEEEEECCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VRLNQWNAGAGFPAIRSDWMRYALGMGDQVSVKIGEREVVGRFENIDTRGAMMLRRKDGI
HEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCHHEEEECCCCC
AEIITAGDVSLGKSD
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]