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The map label for this gene is pcaK [H]

Identifier: 154248276

GI number: 154248276

Start: 4828990

End: 4830333

Strand: Reverse

Name: pcaK [H]

Synonym: Xaut_4356

Alternate gene names: 154248276

Gene position: 4830333-4828990 (Counterclockwise)

Preceding gene: 154248277

Following gene: 154248275

Centisome position: 90.98

GC content: 69.05

Gene sequence:

>1344_bases
ATGGCGGGTGGAAAACAAAGCATCCAGGACCTGCTCGACGGCGAGCCCATACGCGGACGCCAATGGCTGGTCTTTCTTAT
GTGCTTCTTCGTGGCCGCCCTCGACGGCTTCGACACGCAGTGCATCGCCTATACCGGGCCTGCCATCGCCAAGGCGTTCG
GCATGGCGCCGGCGGACATGACCTACATCTTCATCTCGGGCACCCTCGGCATGGCCATCGGTGCCATGGCGCTGGGCTCC
ATCGGCGACAAGATCGGGCGGCGCACCGCCATCCTCGGCGCCATGCTGCTGTTCGGGGTGTTCTCCCTGGCCATCGCGTT
TGCCACCTCGCCCACGCAGATCGTGATGCTGCGCTTCCTCACCGGCCTCGGCATGGGCGGCGCCACCCCGGTGCTGCTGG
CGCTGTCCGCCGAATACGCCCCCGGCCGCGCGCGGGGCACCGTGCTCACCGGTGTGCTGCTGGGCCTGCCGCTCGGCGCG
GTGCTGGGCGGCCTGCTCGCCTCCGGCTGGATGCCGGTGATCGGCTGGCAGGGCATCTATCTGGTGGGCGGCTCGCTGCC
GCTGGTGATGCTGGTGGTCTGCCTCATCGTCCTGCCGGAATCACCCCAGCTTCTGGTTGCTCGCGGCAAGCCCGGCGACC
TGGAGCGAAGCCGGTCACTGGTGGAGAAGATCCTGAAGCGCCCGGTGCCGGCCGGAACCCAGTTCGTGCCCGCCGAGAAG
GTGGCCAAGGGCTCGGTGGCGGCGCTGTTCGCCCCCGCCTACCGCATCCGCACCATCGCGGTGTGGGCGACCTATCTGTT
CAACTGGATCGCCTGGTTCATGCTGCTTTTGTGGCTGCCGACAGTGCTCACCACCTCCGGCCTGCCCGCCGCCACCGCCG
CCTTCGGCACGGTGACCGTGAACACCGCCTTCATCATCTGCGCCATCCCGCTCTCCCTCGTCCTGCCGAAGGTGAACGCA
CGGCTGCTGCTGCTGGGCATGTTCGCCCTGGGCATCGCCATCTGCATTGGTCTGGCGCTGGCCGGTACCAACTGGACGGC
GGTGTTCATCCTGGTGGCGGCGGCGGGCTTCGGCGTCGGCGGCCAGCAGCTGGCGCTGAACTACCTCATCTCGGGCATCT
ACCCCACCGAGCTGCGCGCCACCGGAACCGGCTGGGCCATCGGCATCGGCCGCACCGGCGCCATCGTTGGCTCGGCTACC
GGTGGATGGTTCCTGCAGATGGGCGGGGTGCCCGGCTATTATCTCGGCATCGCCGTGCCGCTCGCCATCGCGGCGCTGGC
GGTGGCGTTGATCCGCTCGCGGCCCTCGGCGCAGCCGGAAGGCGCGCTTTCGGCGGCGCACTGA

Upstream 100 bases:

>100_bases
ATCATGCGCTTCAGACACCCGGCGCGGTCCGACCCGCGCCGGTTCGCCTGATCCCGACACAGCCCAGAACCGCAAGATCA
AGAACCCCTTGGAGGATACA

Downstream 100 bases:

>100_bases
CTGGAAATGGCCGCCGGCGCGATCCGGCGGCCGCACCTTTTCAAGAGGAGACCACAGGTGTCAGACAAGCGCTTTGCCGA
CAAGGTCGCCCTGGTGACCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MAGGKQSIQDLLDGEPIRGRQWLVFLMCFFVAALDGFDTQCIAYTGPAIAKAFGMAPADMTYIFISGTLGMAIGAMALGS
IGDKIGRRTAILGAMLLFGVFSLAIAFATSPTQIVMLRFLTGLGMGGATPVLLALSAEYAPGRARGTVLTGVLLGLPLGA
VLGGLLASGWMPVIGWQGIYLVGGSLPLVMLVVCLIVLPESPQLLVARGKPGDLERSRSLVEKILKRPVPAGTQFVPAEK
VAKGSVAALFAPAYRIRTIAVWATYLFNWIAWFMLLLWLPTVLTTSGLPAATAAFGTVTVNTAFIICAIPLSLVLPKVNA
RLLLLGMFALGIAICIGLALAGTNWTAVFILVAAAGFGVGGQQLALNYLISGIYPTELRATGTGWAIGIGRTGAIVGSAT
GGWFLQMGGVPGYYLGIAVPLAIAALAVALIRSRPSAQPEGALSAAH

Sequences:

>Translated_447_residues
MAGGKQSIQDLLDGEPIRGRQWLVFLMCFFVAALDGFDTQCIAYTGPAIAKAFGMAPADMTYIFISGTLGMAIGAMALGS
IGDKIGRRTAILGAMLLFGVFSLAIAFATSPTQIVMLRFLTGLGMGGATPVLLALSAEYAPGRARGTVLTGVLLGLPLGA
VLGGLLASGWMPVIGWQGIYLVGGSLPLVMLVVCLIVLPESPQLLVARGKPGDLERSRSLVEKILKRPVPAGTQFVPAEK
VAKGSVAALFAPAYRIRTIAVWATYLFNWIAWFMLLLWLPTVLTTSGLPAATAAFGTVTVNTAFIICAIPLSLVLPKVNA
RLLLLGMFALGIAICIGLALAGTNWTAVFILVAAAGFGVGGQQLALNYLISGIYPTELRATGTGWAIGIGRTGAIVGSAT
GGWFLQMGGVPGYYLGIAVPLAIAALAVALIRSRPSAQPEGALSAAH
>Mature_446_residues
AGGKQSIQDLLDGEPIRGRQWLVFLMCFFVAALDGFDTQCIAYTGPAIAKAFGMAPADMTYIFISGTLGMAIGAMALGSI
GDKIGRRTAILGAMLLFGVFSLAIAFATSPTQIVMLRFLTGLGMGGATPVLLALSAEYAPGRARGTVLTGVLLGLPLGAV
LGGLLASGWMPVIGWQGIYLVGGSLPLVMLVVCLIVLPESPQLLVARGKPGDLERSRSLVEKILKRPVPAGTQFVPAEKV
AKGSVAALFAPAYRIRTIAVWATYLFNWIAWFMLLLWLPTVLTTSGLPAATAAFGTVTVNTAFIICAIPLSLVLPKVNAR
LLLLGMFALGIAICIGLALAGTNWTAVFILVAAAGFGVGGQQLALNYLISGIYPTELRATGTGWAIGIGRTGAIVGSATG
GWFLQMGGVPGYYLGIAVPLAIAALAVALIRSRPSAQPEGALSAAH

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=358, Percent_Identity=25.9776536312849, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI73695465, Length=278, Percent_Identity=24.8201438848921, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI7662270, Length=226, Percent_Identity=23.8938053097345, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI216548223, Length=179, Percent_Identity=23.463687150838, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI166064021, Length=340, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI87081723, Length=415, Percent_Identity=31.0843373493976, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082159, Length=403, Percent_Identity=26.0545905707196, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786229, Length=346, Percent_Identity=26.878612716763, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788074, Length=392, Percent_Identity=26.2755102040816, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082231, Length=183, Percent_Identity=26.775956284153, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788068, Length=443, Percent_Identity=23.7020316027088, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1789207, Length=224, Percent_Identity=26.3392857142857, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32564663, Length=386, Percent_Identity=25.1295336787565, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17565978, Length=200, Percent_Identity=31.5, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565976, Length=200, Percent_Identity=26.5, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71988651, Length=249, Percent_Identity=26.9076305220884, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323653, Length=230, Percent_Identity=26.5217391304348, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640198, Length=404, Percent_Identity=25, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640196, Length=404, Percent_Identity=25, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640200, Length=404, Percent_Identity=25, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46231; Mature: 46100

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGGKQSIQDLLDGEPIRGRQWLVFLMCFFVAALDGFDTQCIAYTGPAIAKAFGMAPADM
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCCE
TYIFISGTLGMAIGAMALGSIGDKIGRRTAILGAMLLFGVFSLAIAFATSPTQIVMLRFL
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TGLGMGGATPVLLALSAEYAPGRARGTVLTGVLLGLPLGAVLGGLLASGWMPVIGWQGIY
HHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEE
LVGGSLPLVMLVVCLIVLPESPQLLVARGKPGDLERSRSLVEKILKRPVPAGTQFVPAEK
EECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
VAKGSVAALFAPAYRIRTIAVWATYLFNWIAWFMLLLWLPTVLTTSGLPAATAAFGTVTV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEH
NTAFIICAIPLSLVLPKVNARLLLLGMFALGIAICIGLALAGTNWTAVFILVAAAGFGVG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
GQQLALNYLISGIYPTELRATGTGWAIGIGRTGAIVGSATGGWFLQMGGVPGYYLGIAVP
HHHHHHHHHHHCCCCCCEEECCCCEEEEECCCCCEEECCCCCEEEEECCCCHHHHHHHHH
LAIAALAVALIRSRPSAQPEGALSAAH
HHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGGKQSIQDLLDGEPIRGRQWLVFLMCFFVAALDGFDTQCIAYTGPAIAKAFGMAPADM
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCCE
TYIFISGTLGMAIGAMALGSIGDKIGRRTAILGAMLLFGVFSLAIAFATSPTQIVMLRFL
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TGLGMGGATPVLLALSAEYAPGRARGTVLTGVLLGLPLGAVLGGLLASGWMPVIGWQGIY
HHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEE
LVGGSLPLVMLVVCLIVLPESPQLLVARGKPGDLERSRSLVEKILKRPVPAGTQFVPAEK
EECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
VAKGSVAALFAPAYRIRTIAVWATYLFNWIAWFMLLLWLPTVLTTSGLPAATAAFGTVTV
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEH
NTAFIICAIPLSLVLPKVNARLLLLGMFALGIAICIGLALAGTNWTAVFILVAAAGFGVG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
GQQLALNYLISGIYPTELRATGTGWAIGIGRTGAIVGSATGGWFLQMGGVPGYYLGIAVP
HHHHHHHHHHHCCCCCCEEECCCCEEEEECCCCCEEECCCCCEEEEECCCCHHHHHHHHH
LAIAALAVALIRSRPSAQPEGALSAAH
HHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]