Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 154247751

GI number: 154247751

Start: 4247908

End: 4250226

Strand: Reverse

Name: slt [H]

Synonym: Xaut_3828

Alternate gene names: 154247751

Gene position: 4250226-4247908 (Counterclockwise)

Preceding gene: 154247758

Following gene: 154247743

Centisome position: 80.06

GC content: 69.04

Gene sequence:

>2319_bases
ATGTCGCCTGTTTCGAGCCTGATTTCCGTGAGCGCGCTTGCGCTCGCCCTCGCGTGGAGCGGGGCGATGCCGGCTCTCGC
GGAGACCACGCCGACGGCCAAGCCGGCCTCCGCCAAGGACAGCGCGAAGGACGCCAAGGCCAAGGATTCGAAGGCGAAGG
ATACCCATTCCGCCAAGGACACCAAGGCCAAGGACGCCAAGGCCCACGACAGCAAGGCGAAGGACGCCAAGGCCAAGGAC
ACCAAACCGACCGGCAATGCCAAGGCATCTGCCGACGCCAAGTCCGGCAAGGACGCCAAGGCCCACAAGGATGCGGCAAA
GCCGTCCACCTCCAAGCCCTCGGGCAAGCCGGCCACCGCCCGCGCCCCGGCCGCCACGCCCCAGGTGGCGCCGACGCTGG
GCGCCTCGTCCAACATTCCCGGCGGCGACCTCGCCGCGCTGAAGGTGGCGGTGAACGCCGCCAAGAACGGACGCGCGGCG
GAAGCCATGGGCGCCGCCCAGCAGCTCGACGATCCGGCGGCGCGCAGCCTCGTGACCTGGCTCGTCATCCGCCACGCGCC
CAACGACCTCGGCTTCGACGCCATCAACCAGTTCCTGAAGGACAAGCCCGGCTGGCCGACCCAGAACACCCTGCGCCGCC
GCGCCGAGCGGGTGCTGTTCCAGGAGAACCGCGACCCGGCCAAGGCGCGCGACTTCTTCGCCGAGCAGCCGCCGCTCTCC
GGCGAGGGCAAGATCGCGCTCGCCCGCTACCTCGTGGCCGCCGGCAACCGCTCGGATGCGGCCGACTGGGTGCGAGAGGC
GTGGCGCGAGGATGAGCTGAACGAGAGCTTCGAATCGAAGATCATCGACGAGTTCTCCGGCTTCCTCACCCGCACCGACC
ACAAGCTGCGGGCCGACCGCTTCAGCTACAAGCCGGACCCCGACCGCGCCCTGCGCGCCGCCGCCCGCGCCGGCTCGGAC
ATCGTCGCCCTCACCAAGGCGCGCATGGCCATCGCAAAGAAGGACTCCAACGGCGAGAAGCTACTGGCCCAGGTGCCGTT
CAATTTGTCGAGCGATCCGGCCTATCTGTTCGCCAAGTCCCAACTCGCCCGCCGGGCCGACAAGCCGCAGGACGCCGCGC
GCGCGCTCCTGGCCGGCGCCACCTCCGACCAGGTGCGGGCCGATCCAGACGAATGGTGGATCGAGCGGCGCCTCGTCGCC
CGCGAGCTGCTGGACGCCGGCGATTTCCAGACCGCCTACAAGGTGGCCGCCACCGGCGTGCCGCCCAAGGCCGACAATTA
CCGTGCCGAGCAGCACTTCACCGCCGGCTGGATCGCCCTGCGCTTCCTGAAGGACCCGCGCACCGCCGCCCAGCATTTCG
CCAAGATCGACGACGACCAGAAGCACCCCATCACCCTGTCGCGGGCCTATTACTGGCAGGCGCGGGCGGCCGAGGCCATG
GGCGACGGCGGCCGTGCCCGCGAGCGCTTCCAGGCAGCGGCGCGCTTCCCGGCCACCTATTACGGCCAGCTCTCGCGCAC
CAAGCTCGGCATGGCGCCGGTGGTGCTGCGCTCGCCCTCGCCTTCGTTCGACAGCCGCAACACCTTCGCCCGGCTGGAGC
CGGTGAAGGCCATCCGCATGCTTTATGCGGTGGGCGAGCGCGACATTCCCCTGACCATCTATTACGACCTCGCCTGGCGC
ATGGAGGATCCGGCCCAGCTTGCCATGCTGGCCGACCTCGCCGAGGCAAATGGCGACGCCCGCGGCGCGCTGGTAGTAGG
CAAGGAGGGCCTCGCGGAGGGTCATCCGCTGGAGGCGGAAGCCTTCCCCACCTTCGGCCTGCCCAGCTACACCCCCATCG
GCAATCCGGTGGACAAGGCGGCGATCTATGCGGTGGCGCGGCAGGAAAGCCAGTTCAACGCCCGCACGCTGTCCAGCGCC
AAGGCCATGGGCCTGATGCAGGTGACGCCCGAGGCCGGCCGCGAGGTCACCAAGAAGACCGGCGTGCCCTTCAGCGAATC
CCGGCTCATGAGCGACCAGTCCTACAACGTGCAGTTCGGCGCCGCCGAGTTGGGCGAGCTGGTGCAGAATTACGGCGGCA
ACTACGTGCTGGTGTTCGCCGCCTACAATGCCGGGCGCGGCAGCGTGGCCAAGTGGATCCAGCGCTACGGCGACCCGCGC
GACCCGGACGTGGACGTGGTGGACTGGGTGGAATCCATCCCCTTCTCCGAGACCCGCAACTATGTGCAGCGGGTGATGGA
GAACTACCAAGTCTACAAGGTACGCTTCAACATCCCCTCCAAGCTGCAGATGGAAGCCGACCTGCGCGGCGGCCGCTGA

Upstream 100 bases:

>100_bases
TCAATCTGGTTAGGGTTTCGTCAACACGGCTGGCGGAACATGGGTGAGGGGGAGCCGCAAGATCACATCAAACGACACCA
ACCCGCCGCGAGGACATTGC

Downstream 100 bases:

>100_bases
GGCGGACGGGCTGTTCAAAGGGGCGGGCCTCCAATGACGACCAACATCTCGCCCTGATGCGATGCGCCCCTCTCCCGCTT
GCGGGGGAGGGTTCACCAGC

Product: lytic transglycosylase catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 772; Mature: 771

Protein sequence:

>772_residues
MSPVSSLISVSALALALAWSGAMPALAETTPTAKPASAKDSAKDAKAKDSKAKDTHSAKDTKAKDAKAHDSKAKDAKAKD
TKPTGNAKASADAKSGKDAKAHKDAAKPSTSKPSGKPATARAPAATPQVAPTLGASSNIPGGDLAALKVAVNAAKNGRAA
EAMGAAQQLDDPAARSLVTWLVIRHAPNDLGFDAINQFLKDKPGWPTQNTLRRRAERVLFQENRDPAKARDFFAEQPPLS
GEGKIALARYLVAAGNRSDAADWVREAWREDELNESFESKIIDEFSGFLTRTDHKLRADRFSYKPDPDRALRAAARAGSD
IVALTKARMAIAKKDSNGEKLLAQVPFNLSSDPAYLFAKSQLARRADKPQDAARALLAGATSDQVRADPDEWWIERRLVA
RELLDAGDFQTAYKVAATGVPPKADNYRAEQHFTAGWIALRFLKDPRTAAQHFAKIDDDQKHPITLSRAYYWQARAAEAM
GDGGRARERFQAAARFPATYYGQLSRTKLGMAPVVLRSPSPSFDSRNTFARLEPVKAIRMLYAVGERDIPLTIYYDLAWR
MEDPAQLAMLADLAEANGDARGALVVGKEGLAEGHPLEAEAFPTFGLPSYTPIGNPVDKAAIYAVARQESQFNARTLSSA
KAMGLMQVTPEAGREVTKKTGVPFSESRLMSDQSYNVQFGAAELGELVQNYGGNYVLVFAAYNAGRGSVAKWIQRYGDPR
DPDVDVVDWVESIPFSETRNYVQRVMENYQVYKVRFNIPSKLQMEADLRGGR

Sequences:

>Translated_772_residues
MSPVSSLISVSALALALAWSGAMPALAETTPTAKPASAKDSAKDAKAKDSKAKDTHSAKDTKAKDAKAHDSKAKDAKAKD
TKPTGNAKASADAKSGKDAKAHKDAAKPSTSKPSGKPATARAPAATPQVAPTLGASSNIPGGDLAALKVAVNAAKNGRAA
EAMGAAQQLDDPAARSLVTWLVIRHAPNDLGFDAINQFLKDKPGWPTQNTLRRRAERVLFQENRDPAKARDFFAEQPPLS
GEGKIALARYLVAAGNRSDAADWVREAWREDELNESFESKIIDEFSGFLTRTDHKLRADRFSYKPDPDRALRAAARAGSD
IVALTKARMAIAKKDSNGEKLLAQVPFNLSSDPAYLFAKSQLARRADKPQDAARALLAGATSDQVRADPDEWWIERRLVA
RELLDAGDFQTAYKVAATGVPPKADNYRAEQHFTAGWIALRFLKDPRTAAQHFAKIDDDQKHPITLSRAYYWQARAAEAM
GDGGRARERFQAAARFPATYYGQLSRTKLGMAPVVLRSPSPSFDSRNTFARLEPVKAIRMLYAVGERDIPLTIYYDLAWR
MEDPAQLAMLADLAEANGDARGALVVGKEGLAEGHPLEAEAFPTFGLPSYTPIGNPVDKAAIYAVARQESQFNARTLSSA
KAMGLMQVTPEAGREVTKKTGVPFSESRLMSDQSYNVQFGAAELGELVQNYGGNYVLVFAAYNAGRGSVAKWIQRYGDPR
DPDVDVVDWVESIPFSETRNYVQRVMENYQVYKVRFNIPSKLQMEADLRGGR
>Mature_771_residues
SPVSSLISVSALALALAWSGAMPALAETTPTAKPASAKDSAKDAKAKDSKAKDTHSAKDTKAKDAKAHDSKAKDAKAKDT
KPTGNAKASADAKSGKDAKAHKDAAKPSTSKPSGKPATARAPAATPQVAPTLGASSNIPGGDLAALKVAVNAAKNGRAAE
AMGAAQQLDDPAARSLVTWLVIRHAPNDLGFDAINQFLKDKPGWPTQNTLRRRAERVLFQENRDPAKARDFFAEQPPLSG
EGKIALARYLVAAGNRSDAADWVREAWREDELNESFESKIIDEFSGFLTRTDHKLRADRFSYKPDPDRALRAAARAGSDI
VALTKARMAIAKKDSNGEKLLAQVPFNLSSDPAYLFAKSQLARRADKPQDAARALLAGATSDQVRADPDEWWIERRLVAR
ELLDAGDFQTAYKVAATGVPPKADNYRAEQHFTAGWIALRFLKDPRTAAQHFAKIDDDQKHPITLSRAYYWQARAAEAMG
DGGRARERFQAAARFPATYYGQLSRTKLGMAPVVLRSPSPSFDSRNTFARLEPVKAIRMLYAVGERDIPLTIYYDLAWRM
EDPAQLAMLADLAEANGDARGALVVGKEGLAEGHPLEAEAFPTFGLPSYTPIGNPVDKAAIYAVARQESQFNARTLSSAK
AMGLMQVTPEAGREVTKKTGVPFSESRLMSDQSYNVQFGAAELGELVQNYGGNYVLVFAAYNAGRGSVAKWIQRYGDPRD
PDVDVVDWVESIPFSETRNYVQRVMENYQVYKVRFNIPSKLQMEADLRGGR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=134, Percent_Identity=36.5671641791045, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83941; Mature: 83810

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVSSLISVSALALALAWSGAMPALAETTPTAKPASAKDSAKDAKAKDSKAKDTHSAKD
CCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
TKAKDAKAHDSKAKDAKAKDTKPTGNAKASADAKSGKDAKAHKDAAKPSTSKPSGKPATA
CCCCCCHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
RAPAATPQVAPTLGASSNIPGGDLAALKVAVNAAKNGRAAEAMGAAQQLDDPAARSLVTW
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
LVIRHAPNDLGFDAINQFLKDKPGWPTQNTLRRRAERVLFQENRDPAKARDFFAEQPPLS
HHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
GEGKIALARYLVAAGNRSDAADWVREAWREDELNESFESKIIDEFSGFLTRTDHKLRADR
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FSYKPDPDRALRAAARAGSDIVALTKARMAIAKKDSNGEKLLAQVPFNLSSDPAYLFAKS
CCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHH
QLARRADKPQDAARALLAGATSDQVRADPDEWWIERRLVARELLDAGDFQTAYKVAATGV
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
PPKADNYRAEQHFTAGWIALRFLKDPRTAAQHFAKIDDDQKHPITLSRAYYWQARAAEAM
CCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
GDGGRARERFQAAARFPATYYGQLSRTKLGMAPVVLRSPSPSFDSRNTFARLEPVKAIRM
CCCHHHHHHHHHHHHCCHHHHCCHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
LYAVGERDIPLTIYYDLAWRMEDPAQLAMLADLAEANGDARGALVVGKEGLAEGHPLEAE
HHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
AFPTFGLPSYTPIGNPVDKAAIYAVARQESQFNARTLSSAKAMGLMQVTPEAGREVTKKT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEECCHHHHHHHHHC
GVPFSESRLMSDQSYNVQFGAAELGELVQNYGGNYVLVFAAYNAGRGSVAKWIQRYGDPR
CCCCHHHHHCCCCCCCEEECHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCC
DPDVDVVDWVESIPFSETRNYVQRVMENYQVYKVRFNIPSKLQMEADLRGGR
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCCC
>Mature Secondary Structure 
SPVSSLISVSALALALAWSGAMPALAETTPTAKPASAKDSAKDAKAKDSKAKDTHSAKD
CHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
TKAKDAKAHDSKAKDAKAKDTKPTGNAKASADAKSGKDAKAHKDAAKPSTSKPSGKPATA
CCCCCCHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
RAPAATPQVAPTLGASSNIPGGDLAALKVAVNAAKNGRAAEAMGAAQQLDDPAARSLVTW
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
LVIRHAPNDLGFDAINQFLKDKPGWPTQNTLRRRAERVLFQENRDPAKARDFFAEQPPLS
HHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
GEGKIALARYLVAAGNRSDAADWVREAWREDELNESFESKIIDEFSGFLTRTDHKLRADR
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FSYKPDPDRALRAAARAGSDIVALTKARMAIAKKDSNGEKLLAQVPFNLSSDPAYLFAKS
CCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHH
QLARRADKPQDAARALLAGATSDQVRADPDEWWIERRLVARELLDAGDFQTAYKVAATGV
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
PPKADNYRAEQHFTAGWIALRFLKDPRTAAQHFAKIDDDQKHPITLSRAYYWQARAAEAM
CCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHH
GDGGRARERFQAAARFPATYYGQLSRTKLGMAPVVLRSPSPSFDSRNTFARLEPVKAIRM
CCCHHHHHHHHHHHHCCHHHHCCHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
LYAVGERDIPLTIYYDLAWRMEDPAQLAMLADLAEANGDARGALVVGKEGLAEGHPLEAE
HHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCC
AFPTFGLPSYTPIGNPVDKAAIYAVARQESQFNARTLSSAKAMGLMQVTPEAGREVTKKT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCEEEECCHHHHHHHHHC
GVPFSESRLMSDQSYNVQFGAAELGELVQNYGGNYVLVFAAYNAGRGSVAKWIQRYGDPR
CCCCHHHHHCCCCCCCEEECHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCC
DPDVDVVDWVESIPFSETRNYVQRVMENYQVYKVRFNIPSKLQMEADLRGGR
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]