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The map label for this gene is thyA [H]

Identifier: 154247442

GI number: 154247442

Start: 3913735

End: 3914529

Strand: Reverse

Name: thyA [H]

Synonym: Xaut_3514

Alternate gene names: 154247442

Gene position: 3914529-3913735 (Counterclockwise)

Preceding gene: 154247448

Following gene: 154247441

Centisome position: 73.73

GC content: 64.91

Gene sequence:

>795_bases
ATGCATCAGTATCACGACCTGCTCCGTCGCATCCTGAATGAGGGCGTGCGCAAGGACGACCGCACCGGCACCGGCACCCT
CTCCGTGTTCGGGCACCAGATGCGGTTCGACCTGGGTGAGGGCTTTCCCCTCGTCACCACCAAGAAGCTGCACCTGAAAT
CCATCGTGCACGAGCTTTTGTGGTTCCTCGCCGGCGACACCAATGTCCGCTACCTGAAGGACAACGGCGTCTCCATTTGG
GACGAGTGGGCCGACGAAAAGGGCGATCTCGGCCCGGTCTACGGCCACCAGTGGCGCTCCTGGCCCACGCCCGAAGGCGG
CGTCATCGACCAGATCGCGCAGGTGGTCTCGGACATCCGCCGCAATCCCGATTCGCGCCGCCTCATCGTCACCGCCTGGA
ATCCGGCGGATGTGTCGAAGATGGCGCTGCCACCCTGCCACTGCCTGTTCCAGTTCTATGTGCTGGAGGGCAAGCTCTCC
TGCCAGCTCTACCAGCGCTCGGCGGATGTATTCCTGGGCGTGCCCTTCAACATCGCCTCCTACGCGCTGCTCACCCACAT
GGTGGCGCAGGTGACCGGGCTCGGGGTCGGCGATTTCGTCCACACGCTGGGCGACGCGCACCTTTACGTGAACCATCTGG
AGCAAGCCCGCGCGCAGCTGGAGCGGACACCCCGCGCGCTGCCGCGCCTGGCGCTGAATCCGGCGGTCACCGACCTCTTC
GCCTTCCGTTACGAGGATGTGGAAATTGTGGGCTATGATCCGCACCCGGCGATCAAGGCGCCGGTGGCGGTCTGA

Upstream 100 bases:

>100_bases
CCGATCGTCTAAAGCTCTGGACGAGCGGCGGTGGGCCATTTGCCAGCCGGCCGGCATGGCCTACATCTATGTCCCCAAAG
CCCGGAGGACGGAGGCGCGC

Downstream 100 bases:

>100_bases
ACCCGGGCGCGGGAGCGCCCCTTCGTCCCTGTCCGTTTTTCGGCTCGTGCGTACCGGTCGTTCCAGTGGAGCCGGTTCGG
TTGGGACAACCTGATCGGGA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MHQYHDLLRRILNEGVRKDDRTGTGTLSVFGHQMRFDLGEGFPLVTTKKLHLKSIVHELLWFLAGDTNVRYLKDNGVSIW
DEWADEKGDLGPVYGHQWRSWPTPEGGVIDQIAQVVSDIRRNPDSRRLIVTAWNPADVSKMALPPCHCLFQFYVLEGKLS
CQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLGVGDFVHTLGDAHLYVNHLEQARAQLERTPRALPRLALNPAVTDLF
AFRYEDVEIVGYDPHPAIKAPVAV

Sequences:

>Translated_264_residues
MHQYHDLLRRILNEGVRKDDRTGTGTLSVFGHQMRFDLGEGFPLVTTKKLHLKSIVHELLWFLAGDTNVRYLKDNGVSIW
DEWADEKGDLGPVYGHQWRSWPTPEGGVIDQIAQVVSDIRRNPDSRRLIVTAWNPADVSKMALPPCHCLFQFYVLEGKLS
CQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLGVGDFVHTLGDAHLYVNHLEQARAQLERTPRALPRLALNPAVTDLF
AFRYEDVEIVGYDPHPAIKAPVAV
>Mature_264_residues
MHQYHDLLRRILNEGVRKDDRTGTGTLSVFGHQMRFDLGEGFPLVTTKKLHLKSIVHELLWFLAGDTNVRYLKDNGVSIW
DEWADEKGDLGPVYGHQWRSWPTPEGGVIDQIAQVVSDIRRNPDSRRLIVTAWNPADVSKMALPPCHCLFQFYVLEGKLS
CQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLGVGDFVHTLGDAHLYVNHLEQARAQLERTPRALPRLALNPAVTDLF
AFRYEDVEIVGYDPHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=52.8368794326241, Blast_Score=301, Evalue=6e-82,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=65.9090909090909, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71993377, Length=284, Percent_Identity=46.4788732394366, Blast_Score=271, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=41.0958904109589, Blast_Score=205, Evalue=7e-54,
Organism=Drosophila melanogaster, GI17137556, Length=286, Percent_Identity=55.5944055944056, Blast_Score=314, Evalue=4e-86,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 29763; Mature: 29763

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQYHDLLRRILNEGVRKDDRTGTGTLSVFGHQMRFDLGEGFPLVTTKKLHLKSIVHELL
CCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHEEECCCCCCEEECHHHHHHHHHHHHH
WFLAGDTNVRYLKDNGVSIWDEWADEKGDLGPVYGHQWRSWPTPEGGVIDQIAQVVSDIR
HHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RNPDSRRLIVTAWNPADVSKMALPPCHCLFQFYVLEGKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHCCCHHHHEEECCCCEEEECCHHHHH
YALLTHMVAQVTGLGVGDFVHTLGDAHLYVNHLEQARAQLERTPRALPRLALNPAVTDLF
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHH
AFRYEDVEIVGYDPHPAIKAPVAV
HHEECCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MHQYHDLLRRILNEGVRKDDRTGTGTLSVFGHQMRFDLGEGFPLVTTKKLHLKSIVHELL
CCHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHEEECCCCCCEEECHHHHHHHHHHHHH
WFLAGDTNVRYLKDNGVSIWDEWADEKGDLGPVYGHQWRSWPTPEGGVIDQIAQVVSDIR
HHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RNPDSRRLIVTAWNPADVSKMALPPCHCLFQFYVLEGKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHCCCHHHHEEECCCCEEEECCHHHHH
YALLTHMVAQVTGLGVGDFVHTLGDAHLYVNHLEQARAQLERTPRALPRLALNPAVTDLF
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHH
AFRYEDVEIVGYDPHPAIKAPVAV
HHEECCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA