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The map label for this gene is dapE [H]

Identifier: 154247223

GI number: 154247223

Start: 3658750

End: 3659979

Strand: Direct

Name: dapE [H]

Synonym: Xaut_3295

Alternate gene names: 154247223

Gene position: 3658750-3659979 (Clockwise)

Preceding gene: 154247222

Following gene: 154247224

Centisome position: 68.92

GC content: 68.94

Gene sequence:

>1230_bases
ATGACGACCCCCGACGCCGACACCCTGCGCCACCTCATCGCCGAGCGCGCCGACGCGCAGCACGCGTTCCTGGCGGAGCT
GGTGAAGGTGCCGTCCGACAATCCCCCCGGCGATTGCGCGCCCCATGCCGCCCGCGCCGCCGAGCTGCTGGAAGGGCTCG
GCCATGTGGTGGAGCGCCATGTGGTGCCGGCCGAGCGGGTGAAGGCGGCGGGCATGATCTCGGTCACCAACCTCATCGTG
CGCCATCGTTTCGGGGACGGGCCGGTGATCGCCCTCAACGCCCATGGCGACGTGGTGCCCCCCGGCGAGGGCTGGACCAG
GGATCCCTTCGGCGCCGAGGTGGTGGACGGGCGCATGTATGGCCGTGGCGTCGCCGTCTCCAAGTCGGACTTCGCAACTT
ACGCTTTCGCCCTCGATGCGCTGAAGCGCTCGGGCCTGCCGCTCAAGGGCACGGTGGAACTGCACTTCACCTATGACGAG
GAGATCGGCGGCGAGGTGGGGCCGGGCTGGATCCTCGAACAGGGCATCACCAAGCCGGATTATGCCGTCTCCGCCGGCTT
CACCTATGGCGTGGTGGTGGGCCACAACGGCTGCCTGCACCTGGAGGTGGAGGTGAACGGCAAGTCGGCCCACGCCGCGC
TGCCCTTCACCGGCATCGACGCGCTGGAGGCGGCCAACCACATCCTCACCGCCCTCTATGCCTATCGCGGCACCCTGAAG
GACAAGGTGTCCGAGGTGGCGGGCATCGGCTCGCCGCAGCTGACGGTGGGCCTTATCTCCGGCGGCATCAACACCAATGT
GGTGCCGGACCGCGTCACCTTCCGCCTCGACCGCCGCATCGTGCCCGACGAGAGCCCGGAAGCGGTGGAAGCCGAAGTGC
GCAAGGTGATCTCGGACGCCGCCTCCGCCTTCCCCAAGGCGCGGGTGGAGGTGCGGCGCATCCTGCTGGCGCGGCCTTTG
GTGCCCAGCGAGGGAACGAAGGTGCTGTCGTCGGTGCTGTGCCGGAATGCCTCAGCGGTGACCGGCGAGACGGTGAAGGA
AACCGGCGTTCCCATCTACACGGATGCGCGGCTTTATTCAGAGGCGGGCGTGCCGGTGGTGCTCTACGGCGCCGGCCCCC
ACACCATCGAGGAGGCCAACGGCCACCGGGCGGACGAGAACCTGCTCCTGTCGGACCTCGACAAGGCGACCTTGGTCATC
GCCCTCACCCTGCGCGACCTGCTGGCATAG

Upstream 100 bases:

>100_bases
GTCTTGTGATGGCCGCAGCGCATGCGGGTTGCGGCCATCACGCCTTGTCGTTTGCGTCGAAAGCGCAATAAAAATGTATG
TAAAATCGAAGGAAATCCCC

Downstream 100 bases:

>100_bases
GGGCAAGCGGGGAGGGCCGCGGGCGGCGCATCTCCCCGCGCGGCCTTTTTACCTTCCGGTTTGTGGAAGATGGGGCTTCG
CCGCCGAGGGGGCGAAGCGC

Product: acetylornithine deacetylase or succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTTPDADTLRHLIAERADAQHAFLAELVKVPSDNPPGDCAPHAARAAELLEGLGHVVERHVVPAERVKAAGMISVTNLIV
RHRFGDGPVIALNAHGDVVPPGEGWTRDPFGAEVVDGRMYGRGVAVSKSDFATYAFALDALKRSGLPLKGTVELHFTYDE
EIGGEVGPGWILEQGITKPDYAVSAGFTYGVVVGHNGCLHLEVEVNGKSAHAALPFTGIDALEAANHILTALYAYRGTLK
DKVSEVAGIGSPQLTVGLISGGINTNVVPDRVTFRLDRRIVPDESPEAVEAEVRKVISDAASAFPKARVEVRRILLARPL
VPSEGTKVLSSVLCRNASAVTGETVKETGVPIYTDARLYSEAGVPVVLYGAGPHTIEEANGHRADENLLLSDLDKATLVI
ALTLRDLLA

Sequences:

>Translated_409_residues
MTTPDADTLRHLIAERADAQHAFLAELVKVPSDNPPGDCAPHAARAAELLEGLGHVVERHVVPAERVKAAGMISVTNLIV
RHRFGDGPVIALNAHGDVVPPGEGWTRDPFGAEVVDGRMYGRGVAVSKSDFATYAFALDALKRSGLPLKGTVELHFTYDE
EIGGEVGPGWILEQGITKPDYAVSAGFTYGVVVGHNGCLHLEVEVNGKSAHAALPFTGIDALEAANHILTALYAYRGTLK
DKVSEVAGIGSPQLTVGLISGGINTNVVPDRVTFRLDRRIVPDESPEAVEAEVRKVISDAASAFPKARVEVRRILLARPL
VPSEGTKVLSSVLCRNASAVTGETVKETGVPIYTDARLYSEAGVPVVLYGAGPHTIEEANGHRADENLLLSDLDKATLVI
ALTLRDLLA
>Mature_408_residues
TTPDADTLRHLIAERADAQHAFLAELVKVPSDNPPGDCAPHAARAAELLEGLGHVVERHVVPAERVKAAGMISVTNLIVR
HRFGDGPVIALNAHGDVVPPGEGWTRDPFGAEVVDGRMYGRGVAVSKSDFATYAFALDALKRSGLPLKGTVELHFTYDEE
IGGEVGPGWILEQGITKPDYAVSAGFTYGVVVGHNGCLHLEVEVNGKSAHAALPFTGIDALEAANHILTALYAYRGTLKD
KVSEVAGIGSPQLTVGLISGGINTNVVPDRVTFRLDRRIVPDESPEAVEAEVRKVISDAASAFPKARVEVRRILLARPLV
PSEGTKVLSSVLCRNASAVTGETVKETGVPIYTDARLYSEAGVPVVLYGAGPHTIEEANGHRADENLLLSDLDKATLVIA
LTLRDLLA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501901, Length=321, Percent_Identity=24.9221183800623, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1790395, Length=263, Percent_Identity=28.5171102661597, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788816, Length=397, Percent_Identity=23.6775818639798, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789236, Length=317, Percent_Identity=26.813880126183, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24649206, Length=308, Percent_Identity=29.8701298701299, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356353, Length=310, Percent_Identity=25.8064516129032, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24649202, Length=306, Percent_Identity=29.0849673202614, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24649204, Length=306, Percent_Identity=29.0849673202614, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24649210, Length=301, Percent_Identity=26.2458471760797, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24649208, Length=213, Percent_Identity=29.1079812206573, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649212, Length=293, Percent_Identity=28.6689419795222, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 43390; Mature: 43259

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPDADTLRHLIAERADAQHAFLAELVKVPSDNPPGDCAPHAARAAELLEGLGHVVERH
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VVPAERVKAAGMISVTNLIVRHRFGDGPVIALNAHGDVVPPGEGWTRDPFGAEVVDGRMY
CCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCEEE
GRGVAVSKSDFATYAFALDALKRSGLPLKGTVELHFTYDEEIGGEVGPGWILEQGITKPD
CCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECHHCCCCCCCCHHHHCCCCCCC
YAVSAGFTYGVVVGHNGCLHLEVEVNGKSAHAALPFTGIDALEAANHILTALYAYRGTLK
CEEECCCEEEEEEECCCEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCHHH
DKVSEVAGIGSPQLTVGLISGGINTNVVPDRVTFRLDRRIVPDESPEAVEAEVRKVISDA
HHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
ASAFPKARVEVRRILLARPLVPSEGTKVLSSVLCRNASAVTGETVKETGVPIYTDARLYS
HHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCHHHH
EAGVPVVLYGAGPHTIEEANGHRADENLLLSDLDKATLVIALTLRDLLA
CCCCCEEEECCCCCCHHHCCCCCCCCCHHHHCCCCHHEEEHHHHHHHHC
>Mature Secondary Structure 
TTPDADTLRHLIAERADAQHAFLAELVKVPSDNPPGDCAPHAARAAELLEGLGHVVERH
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VVPAERVKAAGMISVTNLIVRHRFGDGPVIALNAHGDVVPPGEGWTRDPFGAEVVDGRMY
CCCHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCEEE
GRGVAVSKSDFATYAFALDALKRSGLPLKGTVELHFTYDEEIGGEVGPGWILEQGITKPD
CCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECHHCCCCCCCCHHHHCCCCCCC
YAVSAGFTYGVVVGHNGCLHLEVEVNGKSAHAALPFTGIDALEAANHILTALYAYRGTLK
CEEECCCEEEEEEECCCEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCHHH
DKVSEVAGIGSPQLTVGLISGGINTNVVPDRVTFRLDRRIVPDESPEAVEAEVRKVISDA
HHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
ASAFPKARVEVRRILLARPLVPSEGTKVLSSVLCRNASAVTGETVKETGVPIYTDARLYS
HHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCHHHH
EAGVPVVLYGAGPHTIEEANGHRADENLLLSDLDKATLVIALTLRDLLA
CCCCCEEEECCCCCCHHHCCCCCCCCCHHHHCCCCHHEEEHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA