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The map label for this gene is phaA [H]

Identifier: 154247037

GI number: 154247037

Start: 3447758

End: 3448936

Strand: Reverse

Name: phaA [H]

Synonym: Xaut_3108

Alternate gene names: 154247037

Gene position: 3448936-3447758 (Counterclockwise)

Preceding gene: 154247039

Following gene: 154247036

Centisome position: 64.96

GC content: 68.96

Gene sequence:

>1179_bases
ATGAAGGACGAGGTCGTCATCGTCAGCGCAGCGCGCACCCCGGTCGGGTCGTTCAACGGCGCGCTCGCGCCGTTGCCCGC
CCATGAGCTGGGCGCGATCGCCATCAAGGCGGCGCTGGAGCGCGCCGGACTTCCCGCCGATACCGTCAGCGAAGTGATTC
TCGGTCAGGTGCTGACTGCAGCCCAAGGCCAGAACCCCGCCCGCCAGGCCTCGCTGAAGGCCGGCCTGCCCATCGGCGTC
CCGGCGTGGGAAGTGCAGATGGTGTGCGGCTCCGGGCTGCGCTCCGTGGCGCTGGGCGCTCAGGCCATCGTCAACGGCGA
CAGCGAAATCGTCGTGGCCGGCGGCCAGGAATCCATGAGCCAGTCCACCCACGCGGCCCACGTGCGCAACGGCACCCGCA
TGGGCGGCCTCGAGTTCGTGGACACCATGATCAAGGACGGCCTGTGGGATGCCTTCAATGGCTACCACATGGGCAACACC
GCCGAGAACGTGGCGCGCCAGTTCCAGATCACCCGCCAGGAGCAGGACGAGTTCGCCGTCGCTTCCCAGAACAAGGCGGA
AGCGGCCCAGAAGGGCGGCAAGTTCAAGGAGGAGATCGTCCCCGTCACCATCTCCACCCGCAAGGGCGACGTGGTGGTGG
ACCAGGACGAATACATCCGCCACGGTGCCAGCATCGAGGCCATGACCAAGCTGAAGCCCGCCTTCGCCAAGGACGGCACG
GTGACCGCCGGCAACGCCTCCGGCATCAATGACGGCGCCGCCGCCGTGGTGCTCATGAGCGCCGCCAAGGCCAAGGCCGA
GGGCCGCACCCCGCTGGCCCGCATCGTCTCCTGGGCGCAGGCCGGCGTCGATCCGTCGATCATGGGCACGGGTCCCATCC
CCGCCTCCCGCCTCGCGCTGGAGAAGGCCGGCTGGAGCGCCTCGGACCTCGACCTGGTGGAAGCCAACGAGGCCTTCGCC
GCCCAGGCCATCGCCGTGAACAAGGACCTCGGCTGGGATACCAGTAAGGTGAACGTGAACGGCGGCGCCATCGCCATCGG
CCACCCCATCGGCGCCTCGGGCACCCGCATCCTGGTGACCCTGCTGCACGAGATGCAGAAGCGCGACGCCAAGAAGGGCC
TTGCCACCCTGTGCATCGGCGGCGGCATGGGCATCGCCATGTGCCTTGCGCGGGACTGA

Upstream 100 bases:

>100_bases
CCGGCCACGATAGGGGCCGGTACTTTTCACGGCACTGGCAGCACCGCACTGGCAACGAGGCGCAAAGCCGCCCGCCCGAT
CTTCCCCAGGAGAGACACCC

Downstream 100 bases:

>100_bases
GGGCCTCGCGCGGGACTGATCCCGCCTGAAGCGCAGCGCGTGTGACACCGCCGTCCGGCTCGCCGGGCGGCATGGCTCCA
CAACCGACACGCGCACAACC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKDEVVIVSAARTPVGSFNGALAPLPAHELGAIAIKAALERAGLPADTVSEVILGQVLTAAQGQNPARQASLKAGLPIGV
PAWEVQMVCGSGLRSVALGAQAIVNGDSEIVVAGGQESMSQSTHAAHVRNGTRMGGLEFVDTMIKDGLWDAFNGYHMGNT
AENVARQFQITRQEQDEFAVASQNKAEAAQKGGKFKEEIVPVTISTRKGDVVVDQDEYIRHGASIEAMTKLKPAFAKDGT
VTAGNASGINDGAAAVVLMSAAKAKAEGRTPLARIVSWAQAGVDPSIMGTGPIPASRLALEKAGWSASDLDLVEANEAFA
AQAIAVNKDLGWDTSKVNVNGGAIAIGHPIGASGTRILVTLLHEMQKRDAKKGLATLCIGGGMGIAMCLARD

Sequences:

>Translated_392_residues
MKDEVVIVSAARTPVGSFNGALAPLPAHELGAIAIKAALERAGLPADTVSEVILGQVLTAAQGQNPARQASLKAGLPIGV
PAWEVQMVCGSGLRSVALGAQAIVNGDSEIVVAGGQESMSQSTHAAHVRNGTRMGGLEFVDTMIKDGLWDAFNGYHMGNT
AENVARQFQITRQEQDEFAVASQNKAEAAQKGGKFKEEIVPVTISTRKGDVVVDQDEYIRHGASIEAMTKLKPAFAKDGT
VTAGNASGINDGAAAVVLMSAAKAKAEGRTPLARIVSWAQAGVDPSIMGTGPIPASRLALEKAGWSASDLDLVEANEAFA
AQAIAVNKDLGWDTSKVNVNGGAIAIGHPIGASGTRILVTLLHEMQKRDAKKGLATLCIGGGMGIAMCLARD
>Mature_392_residues
MKDEVVIVSAARTPVGSFNGALAPLPAHELGAIAIKAALERAGLPADTVSEVILGQVLTAAQGQNPARQASLKAGLPIGV
PAWEVQMVCGSGLRSVALGAQAIVNGDSEIVVAGGQESMSQSTHAAHVRNGTRMGGLEFVDTMIKDGLWDAFNGYHMGNT
AENVARQFQITRQEQDEFAVASQNKAEAAQKGGKFKEEIVPVTISTRKGDVVVDQDEYIRHGASIEAMTKLKPAFAKDGT
VTAGNASGINDGAAAVVLMSAAKAKAEGRTPLARIVSWAQAGVDPSIMGTGPIPASRLALEKAGWSASDLDLVEANEAFA
AQAIAVNKDLGWDTSKVNVNGGAIAIGHPIGASGTRILVTLLHEMQKRDAKKGLATLCIGGGMGIAMCLARD

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=59.9489795918367, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI167614485, Length=391, Percent_Identity=45.0127877237852, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI4557237, Length=390, Percent_Identity=45.3846153846154, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI4501853, Length=390, Percent_Identity=40.5128205128205, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI4504327, Length=419, Percent_Identity=32.6968973747017, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI194353979, Length=385, Percent_Identity=28.8311688311688, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI87082165, Length=393, Percent_Identity=57.7608142493639, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=59.0330788804071, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=43.5323383084577, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=42.3645320197044, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=35.1219512195122, Blast_Score=208, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=46.5295629820051, Blast_Score=331, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI25147385, Length=385, Percent_Identity=42.3376623376623, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17535921, Length=395, Percent_Identity=41.2658227848101, Blast_Score=270, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17551802, Length=422, Percent_Identity=31.042654028436, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=396, Percent_Identity=33.0808080808081, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325229, Length=391, Percent_Identity=44.7570332480818, Blast_Score=283, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=35.8585858585859, Blast_Score=210, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=57.9896907216495, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24640423, Length=381, Percent_Identity=47.50656167979, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI17648125, Length=388, Percent_Identity=44.3298969072165, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI17137578, Length=423, Percent_Identity=33.096926713948, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40460; Mature: 40460

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEVVIVSAARTPVGSFNGALAPLPAHELGAIAIKAALERAGLPADTVSEVILGQVLTA
CCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AQGQNPARQASLKAGLPIGVPAWEVQMVCGSGLRSVALGAQAIVNGDSEIVVAGGQESMS
HCCCCHHHHHHHHCCCCCCCCHHEEEEHHCCCHHHHHHCCHHEECCCCCEEEECCHHHHH
QSTHAAHVRNGTRMGGLEFVDTMIKDGLWDAFNGYHMGNTAENVARQFQITRQEQDEFAV
HHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
ASQNKAEAAQKGGKFKEEIVPVTISTRKGDVVVDQDEYIRHGASIEAMTKLKPAFAKDGT
HCCCHHHHHHCCCCCHHHEEEEEEECCCCCEEECCHHHHHCCCCHHHHHHCCHHHCCCCC
VTAGNASGINDGAAAVVLMSAAKAKAEGRTPLARIVSWAQAGVDPSIMGTGPIPASRLAL
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHH
EKAGWSASDLDLVEANEAFAAQAIAVNKDLGWDTSKVNVNGGAIAIGHPIGASGTRILVT
HHCCCCCCCCEEEECCHHHHHEEEEEECCCCCCCCEEEECCCEEEECCCCCCCCHHHHHH
LLHEMQKRDAKKGLATLCIGGGMGIAMCLARD
HHHHHHHHHHHCCCEEEEECCCCCEEEEEECC
>Mature Secondary Structure
MKDEVVIVSAARTPVGSFNGALAPLPAHELGAIAIKAALERAGLPADTVSEVILGQVLTA
CCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AQGQNPARQASLKAGLPIGVPAWEVQMVCGSGLRSVALGAQAIVNGDSEIVVAGGQESMS
HCCCCHHHHHHHHCCCCCCCCHHEEEEHHCCCHHHHHHCCHHEECCCCCEEEECCHHHHH
QSTHAAHVRNGTRMGGLEFVDTMIKDGLWDAFNGYHMGNTAENVARQFQITRQEQDEFAV
HHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
ASQNKAEAAQKGGKFKEEIVPVTISTRKGDVVVDQDEYIRHGASIEAMTKLKPAFAKDGT
HCCCHHHHHHCCCCCHHHEEEEEEECCCCCEEECCHHHHHCCCCHHHHHHCCHHHCCCCC
VTAGNASGINDGAAAVVLMSAAKAKAEGRTPLARIVSWAQAGVDPSIMGTGPIPASRLAL
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHH
EKAGWSASDLDLVEANEAFAAQAIAVNKDLGWDTSKVNVNGGAIAIGHPIGASGTRILVT
HHCCCCCCCCEEEECCHHHHHEEEEEECCCCCCCCEEEECCCEEEECCCCCCCCHHHHHH
LLHEMQKRDAKKGLATLCIGGGMGIAMCLARD
HHHHHHHHHHHCCCEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566717 [H]