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The map label for this gene is prfC [H]

Identifier: 154246587

GI number: 154246587

Start: 2939798

End: 2941405

Strand: Direct

Name: prfC [H]

Synonym: Xaut_2646

Alternate gene names: 154246587

Gene position: 2939798-2941405 (Clockwise)

Preceding gene: 154246586

Following gene: 154246588

Centisome position: 55.37

GC content: 68.35

Gene sequence:

>1608_bases
ATGCTCGACCAGACGCCCAACGCCGCCCTCGCGGACCTGCCGGATTTCGCCCGCCGGCGCACCTTCGCCATCATCTCCCA
TCCGGACGCCGGCAAGACGACGCTGACGGAAAAGCTGCTGCTTGCCTCCGGCGCCATCCGCATGGCCGGCCACGTGAAGG
CGCGCGGCGAGCGCCGCCGCACCCGCTCCGACTGGATGGAGATCGAGCAGCAGCGCGGCATCTCGGTCACCTCCTCGGTG
ATGACCTTCGAGCGCGACGGCATCGTCTTCAACCTGCTCGACACCCCCGGCCACTCGGACTTCTCCGAGGACACCTATCG
CACCCTCTCGGCGGTGGACGCGGCGGTGATGGTGATCGACGCCGCCAAGGGCATCGAGAGCCAGACCCGCAAGCTGTTCG
AGGTCTGCCGCCTGCGCGACATTCCCATCTTCACCTTCGTCAACAAGGTGGACCGCGAGAGCCTCGACCCCATCGCCCTG
ATGGACGAGGTGTCCTCCACCCTCGCCCTCGACCTCACCCCGCTGACCTGGCCCATCGGCATGGGCGTCGATTTCCGTGG
CCTCATCGACATCGCCAACAAGAAGGCCCTGCTCGCCACCGAGCGCGGCGGGCCGCTGGTGCGCGAGGTGCCGTTCGAGA
CGCCGGAGGACCTCATCGACCTCGAAGGGGTGGAGGAGCGCATCATCAACGAGGCCGTGGAGAGCCTGACGCTGGCGCTG
GGCGTGCTGCCGCCGTTCGAGCTGGCCTCGTTCCGCGAGGGGCATCTCTCCCCGGTCTATTTCGGGTCGGCGCTGAAGGA
GGCGGGGGTCGCCGAGCTGCTGTTCGGCCTCGGCTCGGTCGGCCCGAGCCCGCTGCCGCGCTCCGCCGCCGGGGATCGCA
CCGTCGAGCCGGCGGAGGACCTCGTCTCCGGCTTCGTCTTCAAGGTGCAGGCCAACATGGATCCCAACCACCGGGACCGC
GTGGCCTTCGTGCGCCTGTGCTCGGGCCGCTTCAAGCGCGGCATGAAGCTGTTCAACCCGCGCGAGAAGCGCCAGATGGC
CATCGCCAACCCCATCTTCTTCTTCGCCCAGGAGCGCGAGACGGTGGACGAGGCCGTGGCTGGCGACATCATCGGCATTC
CCAACCACGGCATGCTGCGGGTGGGCGATACCCTCTCCGAGGGCGAGACCATCAAGTTCGAGGGCCTTCCGGACTTCGCG
CCGGAAATCCTGCGCCGGGTGCTGCTGTCCGACCCCATGAAGGCCAAGCAGCTGCAGAAGGCGCTGCAGGACATGGCGGA
GGAGGGCGTGGTGCAGGTCATCAAGCCCGTCTCTGATCCCTCTCCTATCGTGGGCGTGGTGGGCACTCTGCAGCTCGACG
TGCTCGCCGCCCGGCTGGAGAAGGAATACGGCGTGCCCATCCGCTACGAGACGGTGTCCTTCGTCACCGCCCGCTGGATC
GTGGGCGAGCGCGCCGAGGTGGACCGCTTCATGGAGGCGAACCGCTCCTCCACCGCCCGCGACCGCGACGAGGCGCCGGT
CTATCTCGCCCGCAGCCAGTGGGTGCTGGACCGCGCCATCGAGGAATGGCCCAAGCTCAAGTTCGTCGCCGTGAAGGAAC
GCGGCTGA

Upstream 100 bases:

>100_bases
CCGCGACGTTTCCGCCGAGGCGGACGTGGGCTGGGGTGCGGCGGGCGGCGCCGCGCCCCAACGCCATTCCATGGCCCGCG
ATTTGTCATTATAAGGCGCC

Downstream 100 bases:

>100_bases
CCTTTCATCCCACCCTGAATTGACTCGCCCTGAATTGACTTGCCCGGCCGCGCGGGGGCGCGGCCGATCTGGAGGACCCC
CATGGCCGACGCCGCCCGCC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MLDQTPNAALADLPDFARRRTFAIISHPDAGKTTLTEKLLLASGAIRMAGHVKARGERRRTRSDWMEIEQQRGISVTSSV
MTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMVIDAAKGIESQTRKLFEVCRLRDIPIFTFVNKVDRESLDPIAL
MDEVSSTLALDLTPLTWPIGMGVDFRGLIDIANKKALLATERGGPLVREVPFETPEDLIDLEGVEERIINEAVESLTLAL
GVLPPFELASFREGHLSPVYFGSALKEAGVAELLFGLGSVGPSPLPRSAAGDRTVEPAEDLVSGFVFKVQANMDPNHRDR
VAFVRLCSGRFKRGMKLFNPREKRQMAIANPIFFFAQERETVDEAVAGDIIGIPNHGMLRVGDTLSEGETIKFEGLPDFA
PEILRRVLLSDPMKAKQLQKALQDMAEEGVVQVIKPVSDPSPIVGVVGTLQLDVLAARLEKEYGVPIRYETVSFVTARWI
VGERAEVDRFMEANRSSTARDRDEAPVYLARSQWVLDRAIEEWPKLKFVAVKERG

Sequences:

>Translated_535_residues
MLDQTPNAALADLPDFARRRTFAIISHPDAGKTTLTEKLLLASGAIRMAGHVKARGERRRTRSDWMEIEQQRGISVTSSV
MTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMVIDAAKGIESQTRKLFEVCRLRDIPIFTFVNKVDRESLDPIAL
MDEVSSTLALDLTPLTWPIGMGVDFRGLIDIANKKALLATERGGPLVREVPFETPEDLIDLEGVEERIINEAVESLTLAL
GVLPPFELASFREGHLSPVYFGSALKEAGVAELLFGLGSVGPSPLPRSAAGDRTVEPAEDLVSGFVFKVQANMDPNHRDR
VAFVRLCSGRFKRGMKLFNPREKRQMAIANPIFFFAQERETVDEAVAGDIIGIPNHGMLRVGDTLSEGETIKFEGLPDFA
PEILRRVLLSDPMKAKQLQKALQDMAEEGVVQVIKPVSDPSPIVGVVGTLQLDVLAARLEKEYGVPIRYETVSFVTARWI
VGERAEVDRFMEANRSSTARDRDEAPVYLARSQWVLDRAIEEWPKLKFVAVKERG
>Mature_535_residues
MLDQTPNAALADLPDFARRRTFAIISHPDAGKTTLTEKLLLASGAIRMAGHVKARGERRRTRSDWMEIEQQRGISVTSSV
MTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMVIDAAKGIESQTRKLFEVCRLRDIPIFTFVNKVDRESLDPIAL
MDEVSSTLALDLTPLTWPIGMGVDFRGLIDIANKKALLATERGGPLVREVPFETPEDLIDLEGVEERIINEAVESLTLAL
GVLPPFELASFREGHLSPVYFGSALKEAGVAELLFGLGSVGPSPLPRSAAGDRTVEPAEDLVSGFVFKVQANMDPNHRDR
VAFVRLCSGRFKRGMKLFNPREKRQMAIANPIFFFAQERETVDEAVAGDIIGIPNHGMLRVGDTLSEGETIKFEGLPDFA
PEILRRVLLSDPMKAKQLQKALQDMAEEGVVQVIKPVSDPSPIVGVVGTLQLDVLAARLEKEYGVPIRYETVSFVTARWI
VGERAEVDRFMEANRSSTARDRDEAPVYLARSQWVLDRAIEEWPKLKFVAVKERG

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=482, Percent_Identity=27.3858921161826, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI19923640, Length=502, Percent_Identity=26.2948207171315, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=27.9898218829517, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI25306287, Length=296, Percent_Identity=31.0810810810811, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI157426893, Length=142, Percent_Identity=33.0985915492958, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI94966754, Length=144, Percent_Identity=31.9444444444444, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI4503483, Length=168, Percent_Identity=30.952380952381, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=32.7586206896552, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=32.7586206896552, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=32.7586206896552, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=524, Percent_Identity=48.4732824427481, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1789738, Length=491, Percent_Identity=28.3095723014257, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788922, Length=172, Percent_Identity=31.9767441860465, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI48994988, Length=207, Percent_Identity=30.9178743961353, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=493, Percent_Identity=26.1663286004057, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17556745, Length=482, Percent_Identity=24.6887966804979, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=144, Percent_Identity=32.6388888888889, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=34.0740740740741, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=34.0740740740741, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17506493, Length=165, Percent_Identity=28.4848484848485, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=483, Percent_Identity=26.0869565217391, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6322359, Length=424, Percent_Identity=26.4150943396226, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=190, Percent_Identity=26.8421052631579, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=166, Percent_Identity=33.1325301204819, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=166, Percent_Identity=33.1325301204819, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24582462, Length=497, Percent_Identity=28.169014084507, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI221458488, Length=392, Percent_Identity=28.8265306122449, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI78706572, Length=157, Percent_Identity=35.031847133758, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585711, Length=157, Percent_Identity=31.8471337579618, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585713, Length=157, Percent_Identity=31.8471337579618, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585709, Length=157, Percent_Identity=33.1210191082803, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574573, Length=167, Percent_Identity=32.9341317365269, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59284; Mature: 59284

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQTPNAALADLPDFARRRTFAIISHPDAGKTTLTEKLLLASGAIRMAGHVKARGERRR
CCCCCCCCHHHCCHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHH
TRSDWMEIEQQRGISVTSSVMTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMVID
HHHHHHHHHHHCCCEEEHHHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEH
AAKGIESQTRKLFEVCRLRDIPIFTFVNKVDRESLDPIALMDEVSSTLALDLTPLTWPIG
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCEEECC
MGVDFRGLIDIANKKALLATERGGPLVREVPFETPEDLIDLEGVEERIINEAVESLTLAL
CCCCHHHHHHHCCCCEEEEECCCCCCEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
GVLPPFELASFREGHLSPVYFGSALKEAGVAELLFGLGSVGPSPLPRSAAGDRTVEPAED
HCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
LVSGFVFKVQANMDPNHRDRVAFVRLCSGRFKRGMKLFNPREKRQMAIANPIFFFAQERE
HHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHCCHHEEEECHHH
TVDEAVAGDIIGIPNHGMLRVGDTLSEGETIKFEGLPDFAPEILRRVLLSDPMKAKQLQK
HHHHHHCCCEEECCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHH
ALQDMAEEGVVQVIKPVSDPSPIVGVVGTLQLDVLAARLEKEYGVPIRYETVSFVTARWI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
VGERAEVDRFMEANRSSTARDRDEAPVYLARSQWVLDRAIEEWPKLKFVAVKERG
HCCHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEEEECCC
>Mature Secondary Structure
MLDQTPNAALADLPDFARRRTFAIISHPDAGKTTLTEKLLLASGAIRMAGHVKARGERRR
CCCCCCCCHHHCCHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHH
TRSDWMEIEQQRGISVTSSVMTFERDGIVFNLLDTPGHSDFSEDTYRTLSAVDAAVMVID
HHHHHHHHHHHCCCEEEHHHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEH
AAKGIESQTRKLFEVCRLRDIPIFTFVNKVDRESLDPIALMDEVSSTLALDLTPLTWPIG
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCEEECC
MGVDFRGLIDIANKKALLATERGGPLVREVPFETPEDLIDLEGVEERIINEAVESLTLAL
CCCCHHHHHHHCCCCEEEEECCCCCCEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
GVLPPFELASFREGHLSPVYFGSALKEAGVAELLFGLGSVGPSPLPRSAAGDRTVEPAED
HCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
LVSGFVFKVQANMDPNHRDRVAFVRLCSGRFKRGMKLFNPREKRQMAIANPIFFFAQERE
HHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHCCHHEEEECHHH
TVDEAVAGDIIGIPNHGMLRVGDTLSEGETIKFEGLPDFAPEILRRVLLSDPMKAKQLQK
HHHHHHCCCEEECCCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHH
ALQDMAEEGVVQVIKPVSDPSPIVGVVGTLQLDVLAARLEKEYGVPIRYETVSFVTARWI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
VGERAEVDRFMEANRSSTARDRDEAPVYLARSQWVLDRAIEEWPKLKFVAVKERG
HCCHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA