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The map label for this gene is fixL [H]

Identifier: 154245689

GI number: 154245689

Start: 1964315

End: 1965820

Strand: Reverse

Name: fixL [H]

Synonym: Xaut_1745

Alternate gene names: 154245689

Gene position: 1965820-1964315 (Counterclockwise)

Preceding gene: 154245691

Following gene: 154245688

Centisome position: 37.03

GC content: 69.26

Gene sequence:

>1506_bases
ATGGACAGCGTGGTGAGCGATACGGCCGCAAGGCGCCCGGCAGGGCGCGCCGCATGGCCTGATGCCCTGGTCGGCGGCGC
GGTGGCGCTTCTCGTCCTCGGGCTCGACGTGGTGGTCGGCTTCCCGCCGTTCCGGGCCGAGGGGCTGATGCTCCAGCTCG
CCGGCGTGATGCTGGTGAGCGGGCGGGGGCTGGTCGCCGGCGGCAGCGCGCTGGCGGTGGCCATCGCGGGCGGGGGTGCG
CTCGCTCTCGTCACCGGAGCGCGAAACTCCGGTGGCGCCTTCGATCCGGCGACACTGGCCCTGTTCATCGCTGTGGGGCT
GGGCATCGCCGTGCTCGGCGAGCGGCTGCGCCGCACGAGGCTCGATGCCGCCGCCCGCAACCGCGACCTGCTGGCCCGCG
AGGCGCACCTCTCCTCCATCCTCGACACCGTGCCCGACGCCATGGTGGTGATCGACGAGCGCGGCATCATGCGCTCCTTC
TCCAGCGCGGCCGAGCGCCTGTTCGGCTACACGGCGGCGGAGGCGGTGGGGCAGAACGTCTCGATCCTCATGCCCACGCC
CCATCGGGAGGCCCACGACGGCTATCTCGCGCGCTACCACACTACCGGGGAGCGTCGCATCATCGGCGTCGGCCGGGTGG
TGGTGGGCGAGCGCAAGGACGGCTCCACCTTTCCCATGGAGCTGGCGGTGGGCGAGATGCGCTCCGCCAGCGAGCGCTTC
TTCACCGGTTTCATCCGCGACCTGACCGAGCGGCAGGAGACCGAGGCGCGCCTGCAGGAACTGCAGGCCGAGCTGGTCCA
CATCTCCCGCCTCACCGCCATGGGCGAGATGGCGTCCACGCTCGCCCACGAGCTGAACCAGCCGCTTTCCGCCATCGCCA
ACTATATCAAGGGCTCGCGGCGCCTTTTGGATGCCGGTACCGTCAAGGTGGAGATGCTGCAGGGGGCGCTGGAAAAGGCC
GGGGAGCAGGCCTTGCGCGCCGGCCAGATCATCCGCCGCCTGCGCGACTTCGTCTCCCGCGGGGAGAGTGAGCGACGGGT
GGAGAGCCTGTCCAAGCTGGTCGAGGAGGCGAGTGCCCTCGCCCTGGTCGGCGCCAAGGAACACGGTATCCAGGTGCGCT
TTCACTATGATCCCAGGTGCGACCTCGTGCTTGCCGACAAAGTGCAGGTCCAGCAGGTATTGCTGAACTTGATGCGCAAC
GCCCTGGAGGCAATGATGGAGGCGCCGCGGCGCCAGCTTCTGGTGAAGACCGAGGCGGCGGAGGACGATATGGTGATGAT
CAGCGTCTCGGATACGGGCCTCGGCATCTCGACCGAGGTTGCACCGCAGCTGTTCACACCCTTTGTCACCACCAAGAAGC
ACGGGATGGGGGTGGGGCTATCCATTTCCCGCACCATCATCGAGGGCCATGGCGGCCGCATCTGGGCCGAGCCCAACCCG
GAGGGCGGCGCGGTGTTCCGCTTCACCCTGCGCGCCGTTGGCGAGGAGGCGGCGCTTCATGACTGA

Upstream 100 bases:

>100_bases
CACTTAAGTCGGTGCCCTTAAGTAGTTTTACGGAGGCCGGAACCGGCTGAAGGGGCAGGGTCGCAAAGATGTTTTGGCAA
GGGACGGCGTGACAGGCGGG

Downstream 100 bases:

>100_bases
TGCACGGATGTCTGCGGTGATCCATGTCATCGATGACGACGAGGCCGTGCGCGAGTCGCTGGCTTTCCTTCTCGCCACCG
CCGGCCACACGGTGCACACC

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MDSVVSDTAARRPAGRAAWPDALVGGAVALLVLGLDVVVGFPPFRAEGLMLQLAGVMLVSGRGLVAGGSALAVAIAGGGA
LALVTGARNSGGAFDPATLALFIAVGLGIAVLGERLRRTRLDAAARNRDLLAREAHLSSILDTVPDAMVVIDERGIMRSF
SSAAERLFGYTAAEAVGQNVSILMPTPHREAHDGYLARYHTTGERRIIGVGRVVVGERKDGSTFPMELAVGEMRSASERF
FTGFIRDLTERQETEARLQELQAELVHISRLTAMGEMASTLAHELNQPLSAIANYIKGSRRLLDAGTVKVEMLQGALEKA
GEQALRAGQIIRRLRDFVSRGESERRVESLSKLVEEASALALVGAKEHGIQVRFHYDPRCDLVLADKVQVQQVLLNLMRN
ALEAMMEAPRRQLLVKTEAAEDDMVMISVSDTGLGISTEVAPQLFTPFVTTKKHGMGVGLSISRTIIEGHGGRIWAEPNP
EGGAVFRFTLRAVGEEAALHD

Sequences:

>Translated_501_residues
MDSVVSDTAARRPAGRAAWPDALVGGAVALLVLGLDVVVGFPPFRAEGLMLQLAGVMLVSGRGLVAGGSALAVAIAGGGA
LALVTGARNSGGAFDPATLALFIAVGLGIAVLGERLRRTRLDAAARNRDLLAREAHLSSILDTVPDAMVVIDERGIMRSF
SSAAERLFGYTAAEAVGQNVSILMPTPHREAHDGYLARYHTTGERRIIGVGRVVVGERKDGSTFPMELAVGEMRSASERF
FTGFIRDLTERQETEARLQELQAELVHISRLTAMGEMASTLAHELNQPLSAIANYIKGSRRLLDAGTVKVEMLQGALEKA
GEQALRAGQIIRRLRDFVSRGESERRVESLSKLVEEASALALVGAKEHGIQVRFHYDPRCDLVLADKVQVQQVLLNLMRN
ALEAMMEAPRRQLLVKTEAAEDDMVMISVSDTGLGISTEVAPQLFTPFVTTKKHGMGVGLSISRTIIEGHGGRIWAEPNP
EGGAVFRFTLRAVGEEAALHD
>Mature_501_residues
MDSVVSDTAARRPAGRAAWPDALVGGAVALLVLGLDVVVGFPPFRAEGLMLQLAGVMLVSGRGLVAGGSALAVAIAGGGA
LALVTGARNSGGAFDPATLALFIAVGLGIAVLGERLRRTRLDAAARNRDLLAREAHLSSILDTVPDAMVVIDERGIMRSF
SSAAERLFGYTAAEAVGQNVSILMPTPHREAHDGYLARYHTTGERRIIGVGRVVVGERKDGSTFPMELAVGEMRSASERF
FTGFIRDLTERQETEARLQELQAELVHISRLTAMGEMASTLAHELNQPLSAIANYIKGSRRLLDAGTVKVEMLQGALEKA
GEQALRAGQIIRRLRDFVSRGESERRVESLSKLVEEASALALVGAKEHGIQVRFHYDPRCDLVLADKVQVQQVLLNLMRN
ALEAMMEAPRRQLLVKTEAAEDDMVMISVSDTGLGISTEVAPQLFTPFVTTKKHGMGVGLSISRTIIEGHGGRIWAEPNP
EGGAVFRFTLRAVGEEAALHD

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=364, Percent_Identity=30.4945054945055, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790436, Length=276, Percent_Identity=29.3478260869565, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790300, Length=223, Percent_Identity=29.5964125560538, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=273, Percent_Identity=27.8388278388278, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081921, Length=122, Percent_Identity=27.8688524590164, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53677; Mature: 53677

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSVVSDTAARRPAGRAAWPDALVGGAVALLVLGLDVVVGFPPFRAEGLMLQLAGVMLVS
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
GRGLVAGGSALAVAIAGGGALALVTGARNSGGAFDPATLALFIAVGLGIAVLGERLRRTR
CCCEEECCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LDAAARNRDLLAREAHLSSILDTVPDAMVVIDERGIMRSFSSAAERLFGYTAAEAVGQNV
HHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
SILMPTPHREAHDGYLARYHTTGERRIIGVGRVVVGERKDGSTFPMELAVGEMRSASERF
EEEECCCCCCCCCCEEEEEEECCCEEEEEECEEEEECCCCCCCCCHHHHHHHHHHHHHHH
FTGFIRDLTERQETEARLQELQAELVHISRLTAMGEMASTLAHELNQPLSAIANYIKGSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
RLLDAGTVKVEMLQGALEKAGEQALRAGQIIRRLRDFVSRGESERRVESLSKLVEEASAL
CEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHE
ALVGAKEHGIQVRFHYDPRCDLVLADKVQVQQVLLNLMRNALEAMMEAPRRQLLVKTEAA
EEEECCCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCC
EDDMVMISVSDTGLGISTEVAPQLFTPFVTTKKHGMGVGLSISRTIIEGHGGRIWAEPNP
CCCEEEEEECCCCCCCCHHHHHHHHCCHHCCCCCCCCCCCEEEHHEEECCCCEEECCCCC
EGGAVFRFTLRAVGEEAALHD
CCCEEEEEEHHHHCCHHCCCC
>Mature Secondary Structure
MDSVVSDTAARRPAGRAAWPDALVGGAVALLVLGLDVVVGFPPFRAEGLMLQLAGVMLVS
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
GRGLVAGGSALAVAIAGGGALALVTGARNSGGAFDPATLALFIAVGLGIAVLGERLRRTR
CCCEEECCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LDAAARNRDLLAREAHLSSILDTVPDAMVVIDERGIMRSFSSAAERLFGYTAAEAVGQNV
HHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCC
SILMPTPHREAHDGYLARYHTTGERRIIGVGRVVVGERKDGSTFPMELAVGEMRSASERF
EEEECCCCCCCCCCEEEEEEECCCEEEEEECEEEEECCCCCCCCCHHHHHHHHHHHHHHH
FTGFIRDLTERQETEARLQELQAELVHISRLTAMGEMASTLAHELNQPLSAIANYIKGSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
RLLDAGTVKVEMLQGALEKAGEQALRAGQIIRRLRDFVSRGESERRVESLSKLVEEASAL
CEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHE
ALVGAKEHGIQVRFHYDPRCDLVLADKVQVQQVLLNLMRNALEAMMEAPRRQLLVKTEAA
EEEECCCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCC
EDDMVMISVSDTGLGISTEVAPQLFTPFVTTKKHGMGVGLSISRTIIEGHGGRIWAEPNP
CCCEEEEEECCCCCCCCHHHHHHHHCCHHCCCCCCCCCCCEEEHHEEECCCCEEECCCCC
EGGAVFRFTLRAVGEEAALHD
CCCEEEEEEHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]