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The map label for this gene is fixJ [H]

Identifier: 154245688

GI number: 154245688

Start: 1963681

End: 1964322

Strand: Reverse

Name: fixJ [H]

Synonym: Xaut_1744

Alternate gene names: 154245688

Gene position: 1964322-1963681 (Counterclockwise)

Preceding gene: 154245689

Following gene: 154245687

Centisome position: 37.0

GC content: 66.04

Gene sequence:

>642_bases
ATGACTGATGCACGGATGTCTGCGGTGATCCATGTCATCGATGACGACGAGGCCGTGCGCGAGTCGCTGGCTTTCCTTCT
CGCCACCGCCGGCCACACGGTGCACACCCACGCCTCGGCCCGGCATTTCCTGGACGCCGGCATGCCGGCCGCCGACCCGT
CCATGAGCTGCATCATCACCGACGTGCGCATGCCGGACATGACCGGCATCGACTTGCTGCAGCATTTGCAGGAGAAGGGC
TCCACCGTGCCGGTGATCGTCGTCACCGGCCATGGCGACGTGCCGCTGGCGGTGGAGGCCATGAAGCTCGGTGCCGCCGA
CTTCCTGGAGAAGCCGTTCGACGATGTGGCCATCCTCGATACGGTGAAGTCCGCGCTGGCGCGGCGCGAGAAGGCCTACC
AGCATGATGCGCTGCGCGAGCAGGTCTCGGAGCGCATCGCCGCCTTGTCCCAGCGCGAGCGCCAGGTGCTGGAATGCCTT
GTGGTGGGCCAGGCCAACAAGACCATCGCCTACGAGCTTGGCATCAGCCCCCGCACCGTGGAAGTCTATCGCGCCAACGT
GATGACCAAGATGAAGGCGCAGAGCCTGTCCGAGCTGGTGCGCATGGCGCTGCTGGCGGGCATCTCGCCCAAGGCGGCAT
AG

Upstream 100 bases:

>100_bases
ATCGAGGGCCATGGCGGCCGCATCTGGGCCGAGCCCAACCCGGAGGGCGGCGCGGTGTTCCGCTTCACCCTGCGCGCCGT
TGGCGAGGAGGCGGCGCTTC

Downstream 100 bases:

>100_bases
GGGTAGCATCATGGGGTGTAGCACTATCGTGCCGCCGCTTGATCTAGCGCAACGCCGGACGCCTCCTCCCAAAGTAGGCT
AAAAGCCAATTGCCCATGTG

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTDARMSAVIHVIDDDEAVRESLAFLLATAGHTVHTHASARHFLDAGMPAADPSMSCIITDVRMPDMTGIDLLQHLQEKG
STVPVIVVTGHGDVPLAVEAMKLGAADFLEKPFDDVAILDTVKSALARREKAYQHDALREQVSERIAALSQRERQVLECL
VVGQANKTIAYELGISPRTVEVYRANVMTKMKAQSLSELVRMALLAGISPKAA

Sequences:

>Translated_213_residues
MTDARMSAVIHVIDDDEAVRESLAFLLATAGHTVHTHASARHFLDAGMPAADPSMSCIITDVRMPDMTGIDLLQHLQEKG
STVPVIVVTGHGDVPLAVEAMKLGAADFLEKPFDDVAILDTVKSALARREKAYQHDALREQVSERIAALSQRERQVLECL
VVGQANKTIAYELGISPRTVEVYRANVMTKMKAQSLSELVRMALLAGISPKAA
>Mature_212_residues
TDARMSAVIHVIDDDEAVRESLAFLLATAGHTVHTHASARHFLDAGMPAADPSMSCIITDVRMPDMTGIDLLQHLQEKGS
TVPVIVVTGHGDVPLAVEAMKLGAADFLEKPFDDVAILDTVKSALARREKAYQHDALREQVSERIAALSQRERQVLECLV
VGQANKTIAYELGISPRTVEVYRANVMTKMKAQSLSELVRMALLAGISPKAA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=35.8585858585859, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790299, Length=193, Percent_Identity=30.5699481865285, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788550, Length=139, Percent_Identity=33.0935251798561, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1788222, Length=208, Percent_Identity=28.3653846153846, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1790437, Length=118, Percent_Identity=34.7457627118644, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1786747, Length=154, Percent_Identity=29.2207792207792, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23082; Mature: 22951

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDARMSAVIHVIDDDEAVRESLAFLLATAGHTVHTHASARHFLDAGMPAADPSMSCIIT
CCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHCCCCCCCCCCEEEEE
DVRMPDMTGIDLLQHLQEKGSTVPVIVVTGHGDVPLAVEAMKLGAADFLEKPFDDVAILD
ECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCHHHHHHCCCCHHHHHH
TVKSALARREKAYQHDALREQVSERIAALSQRERQVLECLVVGQANKTIAYELGISPRTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
EVYRANVMTKMKAQSLSELVRMALLAGISPKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDARMSAVIHVIDDDEAVRESLAFLLATAGHTVHTHASARHFLDAGMPAADPSMSCIIT
CCCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHCCCCCCCCCCEEEEE
DVRMPDMTGIDLLQHLQEKGSTVPVIVVTGHGDVPLAVEAMKLGAADFLEKPFDDVAILD
ECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCHHHHHHCCCCHHHHHH
TVKSALARREKAYQHDALREQVSERIAALSQRERQVLECLVVGQANKTIAYELGISPRTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
EVYRANVMTKMKAQSLSELVRMALLAGISPKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]