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The map label for this gene is fixJ [H]

Identifier: 154245687

GI number: 154245687

Start: 1963130

End: 1963519

Strand: Reverse

Name: fixJ [H]

Synonym: Xaut_1743

Alternate gene names: 154245687

Gene position: 1963519-1963130 (Counterclockwise)

Preceding gene: 154245688

Following gene: 154245686

Centisome position: 36.99

GC content: 65.38

Gene sequence:

>390_bases
ATGATCGTGTCCAACGAGGCGCCTGGCTTCGTCGTCCATGTGGTGGATGACGATCCGGCGGTTTGCAACTCGCTGAAGTT
CGCGCTTGAAGTCGATGGCTTCAACGTGCGGACCTATGCCGGTTCGGCGGATGTGCTCGCGGCCGAGCTGTCCCACGGCG
GCTGCATGATCGTGGACTACAATCTGCCCGGCCTGAACGGCCTCGACCTGCTGCGCGAGCTGGACCGGCGCGACGTGCAT
CTTCCGGCCTTCCTCATCACCAGCAATCCCGCCCTGCATGTGCGCAGCCGCGCCACCGCCCAGGGCGTGGCCATCATCGA
GAAACCCCTCCTTGGCAACCAGCTCAGCGAGGCCGTGCGCGACAGTTTCGCGCGTTCGGGCCGGTGCTGA

Upstream 100 bases:

>100_bases
CCTCCTCCCAAAGTAGGCTAAAAGCCAATTGCCCATGTGTGCTGCTGCGCTCTGCTCGGCCGCGTGGACCCGCGAGGTGG
TCGTGGGAAGGACCGGGGAA

Downstream 100 bases:

>100_bases
ACACGGTTCGCTCCGCTCTGCCTGCCTCTCCCCGGAGCCGGGTCCGGTTCGGTTGACCAAACCCCATCGGTGAATCCGGC
TTCAAACCTCAAAAAAGAGA

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MIVSNEAPGFVVHVVDDDPAVCNSLKFALEVDGFNVRTYAGSADVLAAELSHGGCMIVDYNLPGLNGLDLLRELDRRDVH
LPAFLITSNPALHVRSRATAQGVAIIEKPLLGNQLSEAVRDSFARSGRC

Sequences:

>Translated_129_residues
MIVSNEAPGFVVHVVDDDPAVCNSLKFALEVDGFNVRTYAGSADVLAAELSHGGCMIVDYNLPGLNGLDLLRELDRRDVH
LPAFLITSNPALHVRSRATAQGVAIIEKPLLGNQLSEAVRDSFARSGRC
>Mature_129_residues
MIVSNEAPGFVVHVVDDDPAVCNSLKFALEVDGFNVRTYAGSADVLAAELSHGGCMIVDYNLPGLNGLDLLRELDRRDVH
LPAFLITSNPALHVRSRATAQGVAIIEKPLLGNQLSEAVRDSFARSGRC

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13843; Mature: 13843

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSNEAPGFVVHVVDDDPAVCNSLKFALEVDGFNVRTYAGSADVLAAELSHGGCMIVDY
CEECCCCCCEEEEEECCCHHHHCCEEEEEEECCEEEEEECCCCCEEEEECCCCCEEEEEE
NLPGLNGLDLLRELDRRDVHLPAFLITSNPALHVRSRATAQGVAIIEKPLLGNQLSEAVR
CCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCEEEEECCCCCHHHHHHHH
DSFARSGRC
HHHHHCCCC
>Mature Secondary Structure
MIVSNEAPGFVVHVVDDDPAVCNSLKFALEVDGFNVRTYAGSADVLAAELSHGGCMIVDY
CEECCCCCCEEEEEECCCHHHHCCEEEEEEECCEEEEEECCCCCEEEEECCCCCEEEEEE
NLPGLNGLDLLRELDRRDVHLPAFLITSNPALHVRSRATAQGVAIIEKPLLGNQLSEAVR
CCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCEEEEECCCCCHHHHHHHH
DSFARSGRC
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]