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The map label for this gene is emrB [H]

Identifier: 154245460

GI number: 154245460

Start: 1722617

End: 1724215

Strand: Direct

Name: emrB [H]

Synonym: Xaut_1513

Alternate gene names: 154245460

Gene position: 1722617-1724215 (Clockwise)

Preceding gene: 154245459

Following gene: 154245469

Centisome position: 32.45

GC content: 66.04

Gene sequence:

>1599_bases
ATGTCCACCACCATCACCCAAGGCACGCCCATCGCCCCGCCCCGCGCACCCGCCGCCGCGCCGGGCGTCGAGGGGCGGCG
CATGTTCGCCTTCCTGTGCATGGTGTTCGGCATGTTCATGGCGATCCTGGACATCCAGATCGTCTCCGCCTCCCTGGCCG
AGATCCAGGCCGGGCTTGCCGCCTCCTCGGACGAAATCTCCTGGGTGCAGACCAGCTACCTCATCGCCGAGGTGGTGATG
ATCCCGCTCTCGGGCTTCCTGTCGCGGGCGCTGTCCACGCGCTGGCTGTTCGCGGCCTCGGCGGCGGGCTTTACCTTCAT
GAGCTTCATGTGCGCGACGGCCACGAGCATCGAGCAGATGATCGTCTATCGCGCGCTGCAGGGCTTTCTCGGCGGCGGCA
TGATCCCCACCGTGTTCGCCGCGGCCTATTCGGTGTTTCCGAAGGAAAAGCAGCCCATCGTGGCGCCCATGATCGGCCTC
GTGGCGACGCTGGCGCCCACCATCGGGCCCACCGTGGGCGGCTATCTCACCGACCTGTTCTCCTGGCACTGGCTGTTTCT
CATCAATGTGGGGCCGGGCATCCTGGTGGTGATTGCCTCGGTGACGCTGATCGACTTCGACGAGCCGGACTTCGCCCTTC
TGGACCGCTTCGACTGGTGGGGCCTGCTGTTCATGGCGGGCTTCCTCGGCAGCCTGGAGTTCGTGCTGGAGGAAGGCCCG
ACAAACGACTGGTTCGAGGACAATTACATCCTCGCCTTCGCCGTGGTGTGCGGCGTCTCGGCCTTGGCCTTCTTCGCCCG
CGTGCTGATGGCGAAGGAGCCGGTGGTGGATATCCGCGCCTTCGTGAACCGCAACTTCGCCATCGGCTCGGCCTTCAGCT
TCGTCATGGGCATCGGGCTCTATGGCCTGACCTATCTCTACCCGGTGTATCTGGGGCGGGTGCGGGGCTATTCGGCGCTC
CAGATCGGCGAGACCATGTTCGTCACCGGCGTGTTCATGTTCGTGAGCGCGCCCATCGCCGGGCGGCTGATGACGCGGCT
CGATCCCCGCATCATGATCGGCGCCGCCTTCGCCGGCTTCGCGCTGGGCACGTGGTGGATCACCTTCATCACCAAGGACT
GGGATTTCTGGGAGCTGCTGATCCCGCAGATGCTGCGCGGCACCTCGCTGATGATGGCCATGATCCCCATCAACAACCTG
TCGCTGGGCACGCTGCCGCCGGCACAGCTGAAGAATGCCTCGGGCCTGTTCAACCTCACCCGCAATCTCGGCGGGGCGGT
GGGGCTCGCCCTCATCAACACCGTGCTCAACAACCGCTGGGACCTGCACCTCGCCCGCCTGCACGAGAGCGTGGAATGGG
GCAACCAGGCGGCGCTGGAACAGCTCGACGGACTTACGAAGACCTTCGAGGGGCTGGGCTCGCTTGCCTCCGACGCGGCG
CTGAAGCAGATGGCGCTCATCGTGCGGCGCGAGGCGTTGGTGATGTCTTTTGCCGACGTGTTCTACCTGCTCACCTTCGT
GTTCCTCGCTTTGGTGGTGGCCGCCCCGCTCATGCGCCGGCCCAGGCCCGGCGGGGCAGGGGGCGGCGGCGGGCATTGA

Upstream 100 bases:

>100_bases
AAGGGCGCAACCTGCGGCCCGGCATGTCGGTGACCGCCGTGGTCGATACCCGCGCCGACGCCTCCTGAGCGCCGGCCGTT
CGGGCCCAAGGACGAACACC

Downstream 100 bases:

>100_bases
GCGGACCCGTCACCCGAAGATGTCGGCGGTGATCGCTGCGTACCAGCCCATGTTCTCATCCTGCGTCGCCCGGCGCAGGG
CGGGGGGCTCGTTGGTCTGG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MSTTITQGTPIAPPRAPAAAPGVEGRRMFAFLCMVFGMFMAILDIQIVSASLAEIQAGLAASSDEISWVQTSYLIAEVVM
IPLSGFLSRALSTRWLFAASAAGFTFMSFMCATATSIEQMIVYRALQGFLGGGMIPTVFAAAYSVFPKEKQPIVAPMIGL
VATLAPTIGPTVGGYLTDLFSWHWLFLINVGPGILVVIASVTLIDFDEPDFALLDRFDWWGLLFMAGFLGSLEFVLEEGP
TNDWFEDNYILAFAVVCGVSALAFFARVLMAKEPVVDIRAFVNRNFAIGSAFSFVMGIGLYGLTYLYPVYLGRVRGYSAL
QIGETMFVTGVFMFVSAPIAGRLMTRLDPRIMIGAAFAGFALGTWWITFITKDWDFWELLIPQMLRGTSLMMAMIPINNL
SLGTLPPAQLKNASGLFNLTRNLGGAVGLALINTVLNNRWDLHLARLHESVEWGNQAALEQLDGLTKTFEGLGSLASDAA
LKQMALIVRREALVMSFADVFYLLTFVFLALVVAAPLMRRPRPGGAGGGGGH

Sequences:

>Translated_532_residues
MSTTITQGTPIAPPRAPAAAPGVEGRRMFAFLCMVFGMFMAILDIQIVSASLAEIQAGLAASSDEISWVQTSYLIAEVVM
IPLSGFLSRALSTRWLFAASAAGFTFMSFMCATATSIEQMIVYRALQGFLGGGMIPTVFAAAYSVFPKEKQPIVAPMIGL
VATLAPTIGPTVGGYLTDLFSWHWLFLINVGPGILVVIASVTLIDFDEPDFALLDRFDWWGLLFMAGFLGSLEFVLEEGP
TNDWFEDNYILAFAVVCGVSALAFFARVLMAKEPVVDIRAFVNRNFAIGSAFSFVMGIGLYGLTYLYPVYLGRVRGYSAL
QIGETMFVTGVFMFVSAPIAGRLMTRLDPRIMIGAAFAGFALGTWWITFITKDWDFWELLIPQMLRGTSLMMAMIPINNL
SLGTLPPAQLKNASGLFNLTRNLGGAVGLALINTVLNNRWDLHLARLHESVEWGNQAALEQLDGLTKTFEGLGSLASDAA
LKQMALIVRREALVMSFADVFYLLTFVFLALVVAAPLMRRPRPGGAGGGGGH
>Mature_531_residues
STTITQGTPIAPPRAPAAAPGVEGRRMFAFLCMVFGMFMAILDIQIVSASLAEIQAGLAASSDEISWVQTSYLIAEVVMI
PLSGFLSRALSTRWLFAASAAGFTFMSFMCATATSIEQMIVYRALQGFLGGGMIPTVFAAAYSVFPKEKQPIVAPMIGLV
ATLAPTIGPTVGGYLTDLFSWHWLFLINVGPGILVVIASVTLIDFDEPDFALLDRFDWWGLLFMAGFLGSLEFVLEEGPT
NDWFEDNYILAFAVVCGVSALAFFARVLMAKEPVVDIRAFVNRNFAIGSAFSFVMGIGLYGLTYLYPVYLGRVRGYSALQ
IGETMFVTGVFMFVSAPIAGRLMTRLDPRIMIGAAFAGFALGTWWITFITKDWDFWELLIPQMLRGTSLMMAMIPINNLS
LGTLPPAQLKNASGLFNLTRNLGGAVGLALINTVLNNRWDLHLARLHESVEWGNQAALEQLDGLTKTFEGLGSLASDAAL
KQMALIVRREALVMSFADVFYLLTFVFLALVVAAPLMRRPRPGGAGGGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=478, Percent_Identity=30.1255230125523, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI1788710, Length=519, Percent_Identity=27.9383429672447, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1790195, Length=428, Percent_Identity=25.4672897196262, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788392, Length=399, Percent_Identity=27.0676691729323, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=25.3588516746411, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57678; Mature: 57546

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTITQGTPIAPPRAPAAAPGVEGRRMFAFLCMVFGMFMAILDIQIVSASLAEIQAGLA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASSDEISWVQTSYLIAEVVMIPLSGFLSRALSTRWLFAASAAGFTFMSFMCATATSIEQM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYRALQGFLGGGMIPTVFAAAYSVFPKEKQPIVAPMIGLVATLAPTIGPTVGGYLTDLF
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SWHWLFLINVGPGILVVIASVTLIDFDEPDFALLDRFDWWGLLFMAGFLGSLEFVLEEGP
HHHHHEEEECCCHHHHHHHHHHEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
TNDWFEDNYILAFAVVCGVSALAFFARVLMAKEPVVDIRAFVNRNFAIGSAFSFVMGIGL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
YGLTYLYPVYLGRVRGYSALQIGETMFVTGVFMFVSAPIAGRLMTRLDPRIMIGAAFAGF
HHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCHHHHHHHHCCCHHEEHHHHHHH
ALGTWWITFITKDWDFWELLIPQMLRGTSLMMAMIPINNLSLGTLPPAQLKNASGLFNLT
HHHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCHHHHCCCCHHHHHH
RNLGGAVGLALINTVLNNRWDLHLARLHESVEWGNQAALEQLDGLTKTFEGLGSLASDAA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LKQMALIVRREALVMSFADVFYLLTFVFLALVVAAPLMRRPRPGGAGGGGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
STTITQGTPIAPPRAPAAAPGVEGRRMFAFLCMVFGMFMAILDIQIVSASLAEIQAGLA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASSDEISWVQTSYLIAEVVMIPLSGFLSRALSTRWLFAASAAGFTFMSFMCATATSIEQM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVYRALQGFLGGGMIPTVFAAAYSVFPKEKQPIVAPMIGLVATLAPTIGPTVGGYLTDLF
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SWHWLFLINVGPGILVVIASVTLIDFDEPDFALLDRFDWWGLLFMAGFLGSLEFVLEEGP
HHHHHEEEECCCHHHHHHHHHHEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
TNDWFEDNYILAFAVVCGVSALAFFARVLMAKEPVVDIRAFVNRNFAIGSAFSFVMGIGL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
YGLTYLYPVYLGRVRGYSALQIGETMFVTGVFMFVSAPIAGRLMTRLDPRIMIGAAFAGF
HHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCHHHHHHHHCCCHHEEHHHHHHH
ALGTWWITFITKDWDFWELLIPQMLRGTSLMMAMIPINNLSLGTLPPAQLKNASGLFNLT
HHHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCHHHHCCCCHHHHHH
RNLGGAVGLALINTVLNNRWDLHLARLHESVEWGNQAALEQLDGLTKTFEGLGSLASDAA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LKQMALIVRREALVMSFADVFYLLTFVFLALVVAAPLMRRPRPGGAGGGGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]