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The map label for this gene is rutF [H]

Identifier: 154244884

GI number: 154244884

Start: 1054449

End: 1055009

Strand: Direct

Name: rutF [H]

Synonym: Xaut_0934

Alternate gene names: 154244884

Gene position: 1054449-1055009 (Clockwise)

Preceding gene: 154244883

Following gene: 154244885

Centisome position: 19.86

GC content: 67.74

Gene sequence:

>561_bases
ATGACACAGAACATCAACCAGCGCCTTGACTGGGCGTCGAACGAAGCCCTGCGCGAGCTGCCCACCGTCAGCGCGGATGA
CCACCGCTCCGGCATGCGCCACCTGGCATCGGGTGTGTCCATCATCACCAGCCGCGACGGCGAGACCGTGGTGGGGCTCA
CCGCCACGGCGGTGTGCTCGGTCACTGTCGATCCGCCCCGCATGGTGGTTCTGGTCAACAAGAAGGTGGCCGCCGCGGAC
ACCATTCTGGCCAGCGGCGCCTTGTGCATCAACGTGCTCGCCGCCAGCCAGGCGCATCTCGCCAAGGTGTTCGCCGGCAT
GGTGGAGGGGGTGTTCGGCCCCGCCCGCTTCGAGCATGGCAACTGGCACAGCCGCGTCTCCGGCGCGCCCGTGCTTGAGG
GTGCGCTCGCCACGTTCGACTGCCGCGTGGTCAAGGTATTCGACGAGAGCACCCATCACGCCCTGCTGTGCGAGGTGCTT
CACGCCTCCGAGCCGCCGGGCGGCGAGCCGCTGATCTATGTGAACGGCGCTTTCTGTCGCGTCAGCCCCCTGGAAGCGTG
A

Upstream 100 bases:

>100_bases
AGGACAATATCGGCGATCTCCTCGGCGGACAGATGCTCGGCAATCCCATGCCGGCGGCGAACCCGTTCGGCGTTCGGCCG
TGAGCAGGGGGGCTCCTTCA

Downstream 100 bases:

>100_bases
GATGAGCCAGTCCTTCACCGAATCCGAAACCAGCCGCTTCGTCCGCGTCCGCGAAGGCGAGGGCGAACTCAATATCCACT
ATAACGACTGCGATCCGGGC

Product: flavin reductase domain-containing protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MTQNINQRLDWASNEALRELPTVSADDHRSGMRHLASGVSIITSRDGETVVGLTATAVCSVTVDPPRMVVLVNKKVAAAD
TILASGALCINVLAASQAHLAKVFAGMVEGVFGPARFEHGNWHSRVSGAPVLEGALATFDCRVVKVFDESTHHALLCEVL
HASEPPGGEPLIYVNGAFCRVSPLEA

Sequences:

>Translated_186_residues
MTQNINQRLDWASNEALRELPTVSADDHRSGMRHLASGVSIITSRDGETVVGLTATAVCSVTVDPPRMVVLVNKKVAAAD
TILASGALCINVLAASQAHLAKVFAGMVEGVFGPARFEHGNWHSRVSGAPVLEGALATFDCRVVKVFDESTHHALLCEVL
HASEPPGGEPLIYVNGAFCRVSPLEA
>Mature_185_residues
TQNINQRLDWASNEALRELPTVSADDHRSGMRHLASGVSIITSRDGETVVGLTATAVCSVTVDPPRMVVLVNKKVAAADT
ILASGALCINVLAASQAHLAKVFAGMVEGVFGPARFEHGNWHSRVSGAPVLEGALATFDCRVVKVFDESTHHALLCEVLH
ASEPPGGEPLIYVNGAFCRVSPLEA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=158, Percent_Identity=33.5443037974684, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 19665; Mature: 19533

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNINQRLDWASNEALRELPTVSADDHRSGMRHLASGVSIITSRDGETVVGLTATAVCS
CCCCCCHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEEEEEEEEE
VTVDPPRMVVLVNKKVAAADTILASGALCINVLAASQAHLAKVFAGMVEGVFGPARFEHG
EEECCCEEEEEECCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
NWHSRVSGAPVLEGALATFDCRVVKVFDESTHHALLCEVLHASEPPGGEPLIYVNGAFCR
CCCCCCCCCCHHHCHHEEECEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEE
VSPLEA
ECCCCC
>Mature Secondary Structure 
TQNINQRLDWASNEALRELPTVSADDHRSGMRHLASGVSIITSRDGETVVGLTATAVCS
CCCCCHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEEEEEEEEE
VTVDPPRMVVLVNKKVAAADTILASGALCINVLAASQAHLAKVFAGMVEGVFGPARFEHG
EEECCCEEEEEECCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
NWHSRVSGAPVLEGALATFDCRVVKVFDESTHHALLCEVLHASEPPGGEPLIYVNGAFCR
CCCCCCCCCCHHHCHHEEECEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEE
VSPLEA
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA