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The map label for this gene is lutR [H]

Identifier: 154244477

GI number: 154244477

Start: 586317

End: 586994

Strand: Direct

Name: lutR [H]

Synonym: Xaut_0520

Alternate gene names: 154244477

Gene position: 586317-586994 (Clockwise)

Preceding gene: 154244474

Following gene: 154244480

Centisome position: 11.04

GC content: 70.35

Gene sequence:

>678_bases
GTGCGTGGCGAGCGGACCCCGAGGCTCAGGGAGGCGCTGGAGGACGACATCGTCTCCGGCCGGCTGCGGCCGGGACAGAG
GCTCGACGAGGTGGGGCTGGCCGCGCGCTTCGGCGTATCGCGGACGCCGATCCGCGAGGCCTTGATCCAGCTGTCCGCCT
CGGGCCTGATCGAGATCCGCCCCCGCCGCGGCGCCTTCGTGGTGCTGCTCGGCCCGCGCGAGCTTCTGGAATCCTTCGAG
CTGATGGAGGAGCTGGAAGTCGCCTGCGCGCGTCTGGCCGCCCAGCGCATGGGAGATGCCGATCGCGCCGCACTGGCCAG
CGCCCATGCCGCGTGCGGCGCTGCGCTCGCAGGTGGAGATGTTCCAACATATTACAGCGCCAACGCCGCGTTCCACGCCG
CTATTCATGCCGCCACCGGCAATCGCGTGATCCATGGCGAAGCCTCGCGCCTGCAGCGTGCGCTGCAGCCCTATAACCGG
CTGCAGCTCGACGTCCCGCGTCGCCTGGAAGCGTCCTTCGCCGAGCACGAGGCGATCATGGCGGCGCTTTGTGAAGGCGA
CGGCGTCGGGGCTGCGGAGCGGATGCGGGCCCATGTGCAGGTCCAGGGGGAGCGCTTCATGGCTCTGCTCGCAGCCTTGC
AGGCGCAAGCCGAGGCTTCGTCGGCGAGCGCCCTCTAA

Upstream 100 bases:

>100_bases
AAATGCAGATTGGATTCCTTGTCAATAATCTTGTATGCAAGATTTTGCTGACTGCATCTCTGATGCCGGCCAGTGACGGT
GGAGCGGACGGAGCGGGATC

Downstream 100 bases:

>100_bases
ACTCGGGACGGAGACGGCGCCAGGTCAGGGGAGAGAATCTTCCGGCCGGATATTCCGCCTGATTCTCGCCCGGAAGCGCG
GGCGATCGAGCAGAGGTCCG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MRGERTPRLREALEDDIVSGRLRPGQRLDEVGLAARFGVSRTPIREALIQLSASGLIEIRPRRGAFVVLLGPRELLESFE
LMEELEVACARLAAQRMGDADRAALASAHAACGAALAGGDVPTYYSANAAFHAAIHAATGNRVIHGEASRLQRALQPYNR
LQLDVPRRLEASFAEHEAIMAALCEGDGVGAAERMRAHVQVQGERFMALLAALQAQAEASSASAL

Sequences:

>Translated_225_residues
MRGERTPRLREALEDDIVSGRLRPGQRLDEVGLAARFGVSRTPIREALIQLSASGLIEIRPRRGAFVVLLGPRELLESFE
LMEELEVACARLAAQRMGDADRAALASAHAACGAALAGGDVPTYYSANAAFHAAIHAATGNRVIHGEASRLQRALQPYNR
LQLDVPRRLEASFAEHEAIMAALCEGDGVGAAERMRAHVQVQGERFMALLAALQAQAEASSASAL
>Mature_225_residues
MRGERTPRLREALEDDIVSGRLRPGQRLDEVGLAARFGVSRTPIREALIQLSASGLIEIRPRRGAFVVLLGPRELLESFE
LMEELEVACARLAAQRMGDADRAALASAHAACGAALAGGDVPTYYSANAAFHAAIHAATGNRVIHGEASRLQRALQPYNR
LQLDVPRRLEASFAEHEAIMAALCEGDGVGAAERMRAHVQVQGERFMALLAALQAQAEASSASAL

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787821, Length=188, Percent_Identity=26.5957446808511, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87082149, Length=200, Percent_Identity=31, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24100; Mature: 24100

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGERTPRLREALEDDIVSGRLRPGQRLDEVGLAARFGVSRTPIREALIQLSASGLIEIR
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEE
PRRGAFVVLLGPRELLESFELMEELEVACARLAAQRMGDADRAALASAHAACGAALAGGD
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
VPTYYSANAAFHAAIHAATGNRVIHGEASRLQRALQPYNRLQLDVPRRLEASFAEHEAIM
CCCEEECCCHHHHEEEECCCCEEEECCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
AALCEGDGVGAAERMRAHVQVQGERFMALLAALQAQAEASSASAL
HHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRGERTPRLREALEDDIVSGRLRPGQRLDEVGLAARFGVSRTPIREALIQLSASGLIEIR
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEE
PRRGAFVVLLGPRELLESFELMEELEVACARLAAQRMGDADRAALASAHAACGAALAGGD
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
VPTYYSANAAFHAAIHAATGNRVIHGEASRLQRALQPYNRLQLDVPRRLEASFAEHEAIM
CCCEEECCCHHHHEEEECCCCEEEECCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
AALCEGDGVGAAERMRAHVQVQGERFMALLAALQAQAEASSASAL
HHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]