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The map label for this gene is hyb0 [C]

Identifier: 154244300

GI number: 154244300

Start: 357397

End: 358416

Strand: Reverse

Name: hyb0 [C]

Synonym: Xaut_0343

Alternate gene names: 154244300

Gene position: 358416-357397 (Counterclockwise)

Preceding gene: 154244301

Following gene: 154244299

Centisome position: 6.75

GC content: 70.49

Gene sequence:

>1020_bases
ATGAGCACCCTTGCGCGCGAGCCCTTCACCGTGCTGTGGCTGCAATCGGGCGGCTGCGGCGGCTGCACCATGTCGCTCTT
GTGCGCCGAGGCCCCGGACCTTGCCGCGACGCTGGCGAGCGCGAACATCCGCTTCCTGTGGCACCCAACCCTGTCGGAGG
AGACCGGGGACGAGGCCATCGCCCTGTTCGAGGCAGTGCTCGCGGGCGAGGTCCGGCTCGATGCCCTGTGCATCGAAGGG
GCGCTGCTGCGCGGGCCGAACGGCACCGGGCGCTTCCACACCCTCGCCGGCACCGACACCGCCACCATCGACTGGGCGCG
GCGGCTCGCGGCCGTCGCCGAGCATGTGGTGGCGGTGGGCACCTGTGCCGCTTATGGCGGCGTCACGGCGGCCGGCGTCA
ATCCGGCCGATGCCTGCGGCCTGCAATATGACGGGCGCCGTCCCGGCGGGGCGCTGGGCGCGGCCTTCCGCTCCCGCTCC
GGCCTGCCGGTGGTGAATGTCGCCGGCTGCCCCACCCATCCCAACTGGGTGACCGAGACCTTGATGCTGCTCGCCTCCGA
CCTCATGCGGGCGGAGGACCTCGACAGCTATGGCCGCCCGCGCTTCTACGCCGACCACCTCGTGCACCATGGCTGCCCGC
GCAACGAATATTACGAATACAAGGCCAGCGCCGAGAAGATGAGCGACCTCGGCTGCATGATGGAGCATCTTGGCTGCCTC
GGCACCCAGGCCCATGCCGACTGCAACACCCGGCTTTGGAACGGCGAGGGCTCCTGCACCCGCGGCGGCCACGCCTGCAT
CAACTGCACCGCCCCCGAGTTCGAGGAGCCGGGCCATGCCTTCCTTGAAACCCCGAAGATCGGCGGCATCCCCGTGGGCC
TGCCCACCGACATGCCCAAGGCGTGGTTCATCGCCCTGTCGTCCCTCGCCAAGGCGGCGACCCCGGACCGGCTGAGGCGC
AACGCCACCGCCGACCACGTGCTGACGCCACCTGCCGTGCGGGATATCCGCAAGCGATGA

Upstream 100 bases:

>100_bases
GCTACCGTATCGCCGAGGAGCATGACGGACGGCTGGAAGCCGCCAACCACCCGGAGGGCGGCGCCGTCATCTCCCTTATC
CTCCCGTCAGCGGCCCGGCC

Downstream 100 bases:

>100_bases
GCACAGAGACCAAACGGCTCGTGGTGGGGCCGTTCAACCGGGTGGAAGGCGACCTCGAAGTCCGGCTCGACGTGGCCGAC
GGGGAGGTGCGGCAGGCCTT

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSTLAREPFTVLWLQSGGCGGCTMSLLCAEAPDLAATLASANIRFLWHPTLSEETGDEAIALFEAVLAGEVRLDALCIEG
ALLRGPNGTGRFHTLAGTDTATIDWARRLAAVAEHVVAVGTCAAYGGVTAAGVNPADACGLQYDGRRPGGALGAAFRSRS
GLPVVNVAGCPTHPNWVTETLMLLASDLMRAEDLDSYGRPRFYADHLVHHGCPRNEYYEYKASAEKMSDLGCMMEHLGCL
GTQAHADCNTRLWNGEGSCTRGGHACINCTAPEFEEPGHAFLETPKIGGIPVGLPTDMPKAWFIALSSLAKAATPDRLRR
NATADHVLTPPAVRDIRKR

Sequences:

>Translated_339_residues
MSTLAREPFTVLWLQSGGCGGCTMSLLCAEAPDLAATLASANIRFLWHPTLSEETGDEAIALFEAVLAGEVRLDALCIEG
ALLRGPNGTGRFHTLAGTDTATIDWARRLAAVAEHVVAVGTCAAYGGVTAAGVNPADACGLQYDGRRPGGALGAAFRSRS
GLPVVNVAGCPTHPNWVTETLMLLASDLMRAEDLDSYGRPRFYADHLVHHGCPRNEYYEYKASAEKMSDLGCMMEHLGCL
GTQAHADCNTRLWNGEGSCTRGGHACINCTAPEFEEPGHAFLETPKIGGIPVGLPTDMPKAWFIALSSLAKAATPDRLRR
NATADHVLTPPAVRDIRKR
>Mature_338_residues
STLAREPFTVLWLQSGGCGGCTMSLLCAEAPDLAATLASANIRFLWHPTLSEETGDEAIALFEAVLAGEVRLDALCIEGA
LLRGPNGTGRFHTLAGTDTATIDWARRLAAVAEHVVAVGTCAAYGGVTAAGVNPADACGLQYDGRRPGGALGAAFRSRSG
LPVVNVAGCPTHPNWVTETLMLLASDLMRAEDLDSYGRPRFYADHLVHHGCPRNEYYEYKASAEKMSDLGCMMEHLGCLG
TQAHADCNTRLWNGEGSCTRGGHACINCTAPEFEEPGHAFLETPKIGGIPVGLPTDMPKAWFIALSSLAKAATPDRLRRN
ATADHVLTPPAVRDIRKR

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=267, Percent_Identity=28.8389513108614, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787206, Length=286, Percent_Identity=25.1748251748252, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 36042; Mature: 35911

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00277 STAPH_STREP_TOXIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLAREPFTVLWLQSGGCGGCTMSLLCAEAPDLAATLASANIRFLWHPTLSEETGDEAI
CCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCHHHH
ALFEAVLAGEVRLDALCIEGALLRGPNGTGRFHTLAGTDTATIDWARRLAAVAEHVVAVG
HHHHHHHHCCCEEEEEEECCEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
TCAAYGGVTAAGVNPADACGLQYDGRRPGGALGAAFRSRSGLPVVNVAGCPTHPNWVTET
HHHHHCCEEECCCCCHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHH
LMLLASDLMRAEDLDSYGRPRFYADHLVHHGCPRNEYYEYKASAEKMSDLGCMMEHLGCL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GTQAHADCNTRLWNGEGSCTRGGHACINCTAPEFEEPGHAFLETPKIGGIPVGLPTDMPK
CCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCEECCCCCCCCH
AWFIALSSLAKAATPDRLRRNATADHVLTPPAVRDIRKR
HHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
STLAREPFTVLWLQSGGCGGCTMSLLCAEAPDLAATLASANIRFLWHPTLSEETGDEAI
CCCCCCCEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCHHHH
ALFEAVLAGEVRLDALCIEGALLRGPNGTGRFHTLAGTDTATIDWARRLAAVAEHVVAVG
HHHHHHHHCCCEEEEEEECCEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
TCAAYGGVTAAGVNPADACGLQYDGRRPGGALGAAFRSRSGLPVVNVAGCPTHPNWVTET
HHHHHCCEEECCCCCHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHH
LMLLASDLMRAEDLDSYGRPRFYADHLVHHGCPRNEYYEYKASAEKMSDLGCMMEHLGCL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GTQAHADCNTRLWNGEGSCTRGGHACINCTAPEFEEPGHAFLETPKIGGIPVGLPTDMPK
CCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCEECCCCCCCCH
AWFIALSSLAKAATPDRLRRNATADHVLTPPAVRDIRKR
HHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]