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The map label for this gene is hybC [C]

Identifier: 154244299

GI number: 154244299

Start: 355943

End: 357400

Strand: Reverse

Name: hybC [C]

Synonym: Xaut_0342

Alternate gene names: 154244299

Gene position: 357400-355943 (Counterclockwise)

Preceding gene: 154244300

Following gene: 154244298

Centisome position: 6.73

GC content: 71.88

Gene sequence:

>1458_bases
ATGAGCACAGAGACCAAACGGCTCGTGGTGGGGCCGTTCAACCGGGTGGAAGGCGACCTCGAAGTCCGGCTCGACGTGGC
CGACGGGGAGGTGCGGCAGGCCTTCGTCAATTCGCCCCTGTTCCGCGGTTTCGAGCGCATCCTGGAGGGGCGCGACCCGC
GGGACGCCCTTGTCATCGCGCCGCGCATCTGCGGCATCTGCTCGGTGTCGCAGTCCCATGCGGCGGCGCTGGCGCTCGCC
GGCCTTGAGGGCATCGCGCCCACGGACAATGGCCGGGTCGCCACCAACCTCATCCTCGCCACCGAGAACGTGGCGGACCA
CCTCACCCATTTCCACGTCTTCTTCATGCCGGACTTCGCCCGGGCCATCTATGAGGACCGCCCGTGGTTCGCCACCGCCG
CCCGACGCTTCAAGGCGGCGCAGGGCGAGAGCGTGCGCCGGGCACTCGCCACGCGGGCGACGCTGCTGCACGTGATGGGC
CTGCTCGCCGGACGCTGGCCGCACACGCTGGCGATCCAGCCGGGGGGCGTCGCCAAGGGCGCCGACGCGCGGGACCAGAT
GCGCCTCATCGCCACCCTCGGCGCCGTGCGCGCGGACCTGGAGGAGCGCCTTTATGGCGCTCCGCTGGAACGGGTGGCCG
AGATCGCCGACGTGGCGGAACTGGAAGCATGGCGACAGGCGGGGCCGGACGGCGACTTCCGCCTGTTCCTTGAAATCGCC
GCCGACCTGGATCTGGCCCGCCTCGGCCGCGCCCACGACCGCTTCCTCTCCTACGGTGCCTATGCGCTGGACGAGGGCCG
GCTCTACACCGGCGGCACCTTTGCCAACGGGCGGGCCAAGGGCCTCGACCCGGCCGCCATCACCGAGGACCACAGCTTCG
CCCGCATGGAAGGCCGCGAGGCGCCCCACCCGCCCTTCGAAGGCTCGACCTTCCCCGATGGGGCGGACGAGACCGGCTAC
ACCTGGTGCAAGGCCCCGCGCCTCTCCGGCCTGCCGTTCGAGACCGGCGCCTTCGCCCGTCAGGTGGTGGCCGGCCATCC
CCTCGCCCGCGACCTTGCGGCGAGGGAGGGCGCCAACGTGCGCGCCCGCGTCGTCGGCCGGCTGGTTGAGACGGCGCGCA
CCCTCATCGCCATGGAAGGCTGGGTGCGCCAGCTTCATCCCGGCGCGCCCTGGTGCGCGCAAGGCGCGATGCCGCAGTCC
GGCCGCGCCTTCGGCCTCACGGAAGCCGCGCGCGGGGCGCTGGGCCACTGGATGGTGGTGGAGAAGGGGCGCATCGCCCG
CTACCAGATCATCGCCCCCACCACCTGGAACTTCTCCCCCCGCGATGCGGTTGGCCAGCCGGGGCCACTGGAAACGGCGC
TGGTGGGCGCCCCCATCCGGCAGGGTGAAACCACGCCAGTGAGCGTGCAGCACATCGTGCGCTCGTTCGACCCCTGCATG
GTCTGCACGGTGCATTGA

Upstream 100 bases:

>100_bases
TGTCGTCCCTCGCCAAGGCGGCGACCCCGGACCGGCTGAGGCGCAACGCCACCGCCGACCACGTGCTGACGCCACCTGCC
GTGCGGGATATCCGCAAGCG

Downstream 100 bases:

>100_bases
ATCTCATGGGAACCGAGGTCGCGGGCGAGGAGATCGAGGTTTCCGGCATCGTGCAGGGGGTGGGGTTCCGCCCCTTCGTC
TATCGCCTCGCCCGCCGGCT

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSTETKRLVVGPFNRVEGDLEVRLDVADGEVRQAFVNSPLFRGFERILEGRDPRDALVIAPRICGICSVSQSHAAALALA
GLEGIAPTDNGRVATNLILATENVADHLTHFHVFFMPDFARAIYEDRPWFATAARRFKAAQGESVRRALATRATLLHVMG
LLAGRWPHTLAIQPGGVAKGADARDQMRLIATLGAVRADLEERLYGAPLERVAEIADVAELEAWRQAGPDGDFRLFLEIA
ADLDLARLGRAHDRFLSYGAYALDEGRLYTGGTFANGRAKGLDPAAITEDHSFARMEGREAPHPPFEGSTFPDGADETGY
TWCKAPRLSGLPFETGAFARQVVAGHPLARDLAAREGANVRARVVGRLVETARTLIAMEGWVRQLHPGAPWCAQGAMPQS
GRAFGLTEAARGALGHWMVVEKGRIARYQIIAPTTWNFSPRDAVGQPGPLETALVGAPIRQGETTPVSVQHIVRSFDPCM
VCTVH

Sequences:

>Translated_485_residues
MSTETKRLVVGPFNRVEGDLEVRLDVADGEVRQAFVNSPLFRGFERILEGRDPRDALVIAPRICGICSVSQSHAAALALA
GLEGIAPTDNGRVATNLILATENVADHLTHFHVFFMPDFARAIYEDRPWFATAARRFKAAQGESVRRALATRATLLHVMG
LLAGRWPHTLAIQPGGVAKGADARDQMRLIATLGAVRADLEERLYGAPLERVAEIADVAELEAWRQAGPDGDFRLFLEIA
ADLDLARLGRAHDRFLSYGAYALDEGRLYTGGTFANGRAKGLDPAAITEDHSFARMEGREAPHPPFEGSTFPDGADETGY
TWCKAPRLSGLPFETGAFARQVVAGHPLARDLAAREGANVRARVVGRLVETARTLIAMEGWVRQLHPGAPWCAQGAMPQS
GRAFGLTEAARGALGHWMVVEKGRIARYQIIAPTTWNFSPRDAVGQPGPLETALVGAPIRQGETTPVSVQHIVRSFDPCM
VCTVH
>Mature_484_residues
STETKRLVVGPFNRVEGDLEVRLDVADGEVRQAFVNSPLFRGFERILEGRDPRDALVIAPRICGICSVSQSHAAALALAG
LEGIAPTDNGRVATNLILATENVADHLTHFHVFFMPDFARAIYEDRPWFATAARRFKAAQGESVRRALATRATLLHVMGL
LAGRWPHTLAIQPGGVAKGADARDQMRLIATLGAVRADLEERLYGAPLERVAEIADVAELEAWRQAGPDGDFRLFLEIAA
DLDLARLGRAHDRFLSYGAYALDEGRLYTGGTFANGRAKGLDPAAITEDHSFARMEGREAPHPPFEGSTFPDGADETGYT
WCKAPRLSGLPFETGAFARQVVAGHPLARDLAAREGANVRARVVGRLVETARTLIAMEGWVRQLHPGAPWCAQGAMPQSG
RAFGLTEAARGALGHWMVVEKGRIARYQIIAPTTWNFSPRDAVGQPGPLETALVGAPIRQGETTPVSVQHIVRSFDPCMV
CTVH

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=566, Percent_Identity=25.7950530035336, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787207, Length=289, Percent_Identity=30.7958477508651, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 52597; Mature: 52466

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTETKRLVVGPFNRVEGDLEVRLDVADGEVRQAFVNSPLFRGFERILEGRDPRDALVIA
CCCCCCEEEECCHHHCCCCEEEEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEC
PRICGICSVSQSHAAALALAGLEGIAPTDNGRVATNLILATENVADHLTHFHVFFMPDFA
CHHHCHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECHHHHHHHEEEEEECCHHH
RAIYEDRPWFATAARRFKAAQGESVRRALATRATLLHVMGLLAGRWPHTLAIQPGGVAKG
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
ADARDQMRLIATLGAVRADLEERLYGAPLERVAEIADVAELEAWRQAGPDGDFRLFLEIA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH
ADLDLARLGRAHDRFLSYGAYALDEGRLYTGGTFANGRAKGLDPAAITEDHSFARMEGRE
HCCCHHHHHHHHHHHHHHCCEEECCCEEEECCEECCCCCCCCCCCCCCCCCCHHHHCCCC
APHPPFEGSTFPDGADETGYTWCKAPRLSGLPFETGAFARQVVAGHPLARDLAAREGANV
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCH
RARVVGRLVETARTLIAMEGWVRQLHPGAPWCAQGAMPQSGRAFGLTEAARGALGHWMVV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEECCHHHHHCCCCEEEEE
EKGRIARYQIIAPTTWNFSPRDAVGQPGPLETALVGAPIRQGETTPVSVQHIVRSFDPCM
ECCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCCHHHHHHHHCCCCEE
VCTVH
EEEEC
>Mature Secondary Structure 
STETKRLVVGPFNRVEGDLEVRLDVADGEVRQAFVNSPLFRGFERILEGRDPRDALVIA
CCCCCEEEECCHHHCCCCEEEEEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEC
PRICGICSVSQSHAAALALAGLEGIAPTDNGRVATNLILATENVADHLTHFHVFFMPDFA
CHHHCHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECHHHHHHHEEEEEECCHHH
RAIYEDRPWFATAARRFKAAQGESVRRALATRATLLHVMGLLAGRWPHTLAIQPGGVAKG
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
ADARDQMRLIATLGAVRADLEERLYGAPLERVAEIADVAELEAWRQAGPDGDFRLFLEIA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEH
ADLDLARLGRAHDRFLSYGAYALDEGRLYTGGTFANGRAKGLDPAAITEDHSFARMEGRE
HCCCHHHHHHHHHHHHHHCCEEECCCEEEECCEECCCCCCCCCCCCCCCCCCHHHHCCCC
APHPPFEGSTFPDGADETGYTWCKAPRLSGLPFETGAFARQVVAGHPLARDLAAREGANV
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCH
RARVVGRLVETARTLIAMEGWVRQLHPGAPWCAQGAMPQSGRAFGLTEAARGALGHWMVV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEECCHHHHHCCCCEEEEE
EKGRIARYQIIAPTTWNFSPRDAVGQPGPLETALVGAPIRQGETTPVSVQHIVRSFDPCM
ECCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCCHHHHHHHHCCCCEE
VCTVH
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]