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The map label for this gene is hypF [H]

Identifier: 154244298

GI number: 154244298

Start: 353646

End: 355937

Strand: Reverse

Name: hypF [H]

Synonym: Xaut_0341

Alternate gene names: 154244298

Gene position: 355937-353646 (Counterclockwise)

Preceding gene: 154244299

Following gene: 154244297

Centisome position: 6.7

GC content: 73.12

Gene sequence:

>2292_bases
ATGGGAACCGAGGTCGCGGGCGAGGAGATCGAGGTTTCCGGCATCGTGCAGGGGGTGGGGTTCCGCCCCTTCGTCTATCG
CCTCGCCCGCCGGCTGGACCTCGTCGGAGACGTGCGCAACGCCGGCGACCGCGTCATCATCCGCATCGCCGGCCTGGCTT
CGGCGCGCGATGCCTTCGCGGCGGCGCTGGCGGACGAGGCGCCGGTGCTGGCGCGCGTGGAACAAATGATGCGACGCCCG
GCCCAGGTCCCGGACGGTCCCTTCCGCATCATGGAGAGTGCGGCGGGCACGGTTTCGGTGGGGGTGGTGCCGGATGTGGC
CACCTGCCCCGCCTGCCGAGCCGAGATCGCCGATCCCAAGGCCCGGCGCTTCCGCTACGCCTTCACCAACTGCACCGATT
GCGGTCCGCGCTTCTCCATCGTCACCGGCCTGCCCTACGACCGCCCGGCCACCACCATGGCCGGCTTTCCCATGTGCCCC
GCCTGCCGCGCCGAATATGACGACCCTGCCGACCGCCGCTTCCACGCCCAGCCCATCGCCTGCCCGGACTGCGGCCCGAA
GCTGTGGCTGGAGGGCGACGACGCTGCCGGGGCCGACCCCATCGAGCGTGCCGCCGCCCTGCTGCGCGCCGGGCATATCC
TCGCGATCAAGGGTATCGGCGGCTTCCACATCGCCTGCGACGCCACCGAGGTGGACGCCGTCGCCCTGCTGCGCGCCCGC
AAGCACCGCCCCACCAAGCCGCTGGCCGTGATGGCGGACATGGCCACCGCCCACGGCCTCTGCCTCATCACGCCGGAGGA
GGAGGCGGCGCTCGCCCACCCTTCCGCCCCCATCCTGCTGCTATCCCTGAAGCCCGGTGCGCCACTCGCGCCAGGCATCG
CCCCGGGCCAGCGCCATCTCGGCGTGATGCTTCCCTATACGCCCCTGCACCACCTGCTGCTGGCCGCCGTGGGCCGCCCG
CTGGTGATGACCTCCGGCAACCGCTCCAGCGAACCGCAGATCTTCCGCGACGACGAGGCCCGCGAACGCCTCGCCGGCAT
CGTTGATGCGTTCCTCATGCACGACCGGCCCATCGCCCGCCGCCTCGACGACAGCGTGGCGCGCCATGTAGCGGGCCGGC
TGCGGATCATGCGGCGCGGCCGCGGGCTCGCCCCCATGCCTCTCACCCTGCCTGCCGATTTCGCCGATGCCCCGCCCGTG
CTCGCCCTGGGCGGGGAGCTGAAAAGCGCTCTCTGCCTGACCCATGGCGCCAAAGCACTGCTCTCCCACCATCTCGGCGA
CCTGGACGAGCCGGCCACGGGCGACGCCTTTGAGACCGCGCTTGCCGATTACGCCGCCCTCTTCGCTCACAAGCCTGCGG
CGGTAGCTGTGGACCTGCACCCGGACTACCGCGCCACCCGCCTCGGCGAGGATATGGCCGCCACCCGTAGCCTGCCGCTT
GAGCGGGTGCAGCACCACCATGCCCATATGGCAGCCGCCATGGCCGAGGCCGGCTGGCGGCGGGCGGACGGGCCGGTGGT
GGGCATCGCGCTGGACGGCCTCGGCCTGGGCGAGGACGGCACCGCTTGGGGCGCCGAAATCCTCGTCTGCGATTACCGCG
CCAGCCGGCGCGTCGCCCGCCTCTCCCCCATCCCGCTGGCCGGAGGAGACGCGGCGAGCCGGGAACCGTGGCGGGTGCTC
CTGGCCCATCTCGACCGCGCGCTGGGGCGTTCGGCTGTGGATGGCGACCCGCGCCTCGCGGCGCTGTTGGCCGGCAAGCC
GCTCCTGACCCTGCGCGCCATGATGGACAAGGGGCTGAACGCGCCCCTGGCCTCCTCTGCCGGCCGGCTGTTCGATGCGG
TGGCGGCGGTGCTGGGCCTTGCGCCCGACCGCCTCAGCCACGAGGGTGAGGCCGCCATGGCGCTGGAGGCGGCGGCACAG
GGCGACGCCCCGCCCTACCCCTTCGCCTTGCATGCTGAAGCGGTCCCGATCGAGATCGACCCCGCCCCGCTGTTCAAGGC
GCTGGTGGACGACCTCCACGCCGGGTCGCCCCGCGCCGCCATGGCCGCCGCCTTCCACGCCGGCCTCGCCGAAGCCTTCA
CCGGGGCGGCGGTGCAGGTGGCGGCGAAGGAAGGCCTCACCGCCGTCGCCCTGTCCGGCGGCGTGTTCCAGAATGCCCGG
CTGCTGGAAGAGACGGTGCGGCGGCTTGAGGCCCATGGCCTCATCCCGCTGGTGCCCGGCGACGTACCGGCCAACGATGG
CGGCCTCGCCTTCGGCCAGGCGGTGGTGGCCGCCGCCCGTCGGATGGGGTGA

Upstream 100 bases:

>100_bases
TGGGCGCCCCCATCCGGCAGGGTGAAACCACGCCAGTGAGCGTGCAGCACATCGTGCGCTCGTTCGACCCCTGCATGGTC
TGCACGGTGCATTGAATCTC

Downstream 100 bases:

>100_bases
GGGCGGGCGCCCGGTCTCTTCAGTCACACCCGCCCCGCGTCCCGGTTTTCGTCCCGCGCGAGCGGGGCTATGGTGGCGCC
ATGACCAGCCCGCACCGCTC

Product: (NiFe) hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MGTEVAGEEIEVSGIVQGVGFRPFVYRLARRLDLVGDVRNAGDRVIIRIAGLASARDAFAAALADEAPVLARVEQMMRRP
AQVPDGPFRIMESAAGTVSVGVVPDVATCPACRAEIADPKARRFRYAFTNCTDCGPRFSIVTGLPYDRPATTMAGFPMCP
ACRAEYDDPADRRFHAQPIACPDCGPKLWLEGDDAAGADPIERAAALLRAGHILAIKGIGGFHIACDATEVDAVALLRAR
KHRPTKPLAVMADMATAHGLCLITPEEEAALAHPSAPILLLSLKPGAPLAPGIAPGQRHLGVMLPYTPLHHLLLAAVGRP
LVMTSGNRSSEPQIFRDDEARERLAGIVDAFLMHDRPIARRLDDSVARHVAGRLRIMRRGRGLAPMPLTLPADFADAPPV
LALGGELKSALCLTHGAKALLSHHLGDLDEPATGDAFETALADYAALFAHKPAAVAVDLHPDYRATRLGEDMAATRSLPL
ERVQHHHAHMAAAMAEAGWRRADGPVVGIALDGLGLGEDGTAWGAEILVCDYRASRRVARLSPIPLAGGDAASREPWRVL
LAHLDRALGRSAVDGDPRLAALLAGKPLLTLRAMMDKGLNAPLASSAGRLFDAVAAVLGLAPDRLSHEGEAAMALEAAAQ
GDAPPYPFALHAEAVPIEIDPAPLFKALVDDLHAGSPRAAMAAAFHAGLAEAFTGAAVQVAAKEGLTAVALSGGVFQNAR
LLEETVRRLEAHGLIPLVPGDVPANDGGLAFGQAVVAAARRMG

Sequences:

>Translated_763_residues
MGTEVAGEEIEVSGIVQGVGFRPFVYRLARRLDLVGDVRNAGDRVIIRIAGLASARDAFAAALADEAPVLARVEQMMRRP
AQVPDGPFRIMESAAGTVSVGVVPDVATCPACRAEIADPKARRFRYAFTNCTDCGPRFSIVTGLPYDRPATTMAGFPMCP
ACRAEYDDPADRRFHAQPIACPDCGPKLWLEGDDAAGADPIERAAALLRAGHILAIKGIGGFHIACDATEVDAVALLRAR
KHRPTKPLAVMADMATAHGLCLITPEEEAALAHPSAPILLLSLKPGAPLAPGIAPGQRHLGVMLPYTPLHHLLLAAVGRP
LVMTSGNRSSEPQIFRDDEARERLAGIVDAFLMHDRPIARRLDDSVARHVAGRLRIMRRGRGLAPMPLTLPADFADAPPV
LALGGELKSALCLTHGAKALLSHHLGDLDEPATGDAFETALADYAALFAHKPAAVAVDLHPDYRATRLGEDMAATRSLPL
ERVQHHHAHMAAAMAEAGWRRADGPVVGIALDGLGLGEDGTAWGAEILVCDYRASRRVARLSPIPLAGGDAASREPWRVL
LAHLDRALGRSAVDGDPRLAALLAGKPLLTLRAMMDKGLNAPLASSAGRLFDAVAAVLGLAPDRLSHEGEAAMALEAAAQ
GDAPPYPFALHAEAVPIEIDPAPLFKALVDDLHAGSPRAAMAAAFHAGLAEAFTGAAVQVAAKEGLTAVALSGGVFQNAR
LLEETVRRLEAHGLIPLVPGDVPANDGGLAFGQAVVAAARRMG
>Mature_762_residues
GTEVAGEEIEVSGIVQGVGFRPFVYRLARRLDLVGDVRNAGDRVIIRIAGLASARDAFAAALADEAPVLARVEQMMRRPA
QVPDGPFRIMESAAGTVSVGVVPDVATCPACRAEIADPKARRFRYAFTNCTDCGPRFSIVTGLPYDRPATTMAGFPMCPA
CRAEYDDPADRRFHAQPIACPDCGPKLWLEGDDAAGADPIERAAALLRAGHILAIKGIGGFHIACDATEVDAVALLRARK
HRPTKPLAVMADMATAHGLCLITPEEEAALAHPSAPILLLSLKPGAPLAPGIAPGQRHLGVMLPYTPLHHLLLAAVGRPL
VMTSGNRSSEPQIFRDDEARERLAGIVDAFLMHDRPIARRLDDSVARHVAGRLRIMRRGRGLAPMPLTLPADFADAPPVL
ALGGELKSALCLTHGAKALLSHHLGDLDEPATGDAFETALADYAALFAHKPAAVAVDLHPDYRATRLGEDMAATRSLPLE
RVQHHHAHMAAAMAEAGWRRADGPVVGIALDGLGLGEDGTAWGAEILVCDYRASRRVARLSPIPLAGGDAASREPWRVLL
AHLDRALGRSAVDGDPRLAALLAGKPLLTLRAMMDKGLNAPLASSAGRLFDAVAAVLGLAPDRLSHEGEAAMALEAAAQG
DAPPYPFALHAEAVPIEIDPAPLFKALVDDLHAGSPRAAMAAAFHAGLAEAFTGAAVQVAAKEGLTAVALSGGVFQNARL
LEETVRRLEAHGLIPLVPGDVPANDGGLAFGQAVVAAARRMG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=764, Percent_Identity=44.5026178010471, Blast_Score=533, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 80091; Mature: 79960

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTEVAGEEIEVSGIVQGVGFRPFVYRLARRLDLVGDVRNAGDRVIIRIAGLASARDAFA
CCCCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
AALADEAPVLARVEQMMRRPAQVPDGPFRIMESAAGTVSVGVVPDVATCPACRAEIADPK
HHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCCH
ARRFRYAFTNCTDCGPRFSIVTGLPYDRPATTMAGFPMCPACRAEYDDPADRRFHAQPIA
HHHEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
CPDCGPKLWLEGDDAAGADPIERAAALLRAGHILAIKGIGGFHIACDATEVDAVALLRAR
CCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHHHHHHH
KHRPTKPLAVMADMATAHGLCLITPEEEAALAHPSAPILLLSLKPGAPLAPGIAPGQRHL
CCCCCCCHHHHHHHHHHCCEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
GVMLPYTPLHHLLLAAVGRPLVMTSGNRSSEPQIFRDDEARERLAGIVDAFLMHDRPIAR
EEECCCHHHHHHHHHHCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCHHHH
RLDDSVARHVAGRLRIMRRGRGLAPMPLTLPADFADAPPVLALGGELKSALCLTHGAKAL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
LSHHLGDLDEPATGDAFETALADYAALFAHKPAAVAVDLHPDYRATRLGEDMAATRSLPL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCHHHHHHCCCH
ERVQHHHAHMAAAMAEAGWRRADGPVVGIALDGLGLGEDGTAWGAEILVCDYRASRRVAR
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHCCHHH
LSPIPLAGGDAASREPWRVLLAHLDRALGRSAVDGDPRLAALLAGKPLLTLRAMMDKGLN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHCCCHHHHHHHHHHCCCC
APLASSAGRLFDAVAAVLGLAPDRLSHEGEAAMALEAAAQGDAPPYPFALHAEAVPIEID
CCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCEEEEECC
PAPLFKALVDDLHAGSPRAAMAAAFHAGLAEAFTGAAVQVAAKEGLTAVALSGGVFQNAR
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHCCCEEEEECCCCHHHHH
LLEETVRRLEAHGLIPLVPGDVPANDGGLAFGQAVVAAARRMG
HHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTEVAGEEIEVSGIVQGVGFRPFVYRLARRLDLVGDVRNAGDRVIIRIAGLASARDAFA
CCCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
AALADEAPVLARVEQMMRRPAQVPDGPFRIMESAAGTVSVGVVPDVATCPACRAEIADPK
HHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCCH
ARRFRYAFTNCTDCGPRFSIVTGLPYDRPATTMAGFPMCPACRAEYDDPADRRFHAQPIA
HHHEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
CPDCGPKLWLEGDDAAGADPIERAAALLRAGHILAIKGIGGFHIACDATEVDAVALLRAR
CCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHHHHHHH
KHRPTKPLAVMADMATAHGLCLITPEEEAALAHPSAPILLLSLKPGAPLAPGIAPGQRHL
CCCCCCCHHHHHHHHHHCCEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
GVMLPYTPLHHLLLAAVGRPLVMTSGNRSSEPQIFRDDEARERLAGIVDAFLMHDRPIAR
EEECCCHHHHHHHHHHCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCHHHH
RLDDSVARHVAGRLRIMRRGRGLAPMPLTLPADFADAPPVLALGGELKSALCLTHGAKAL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
LSHHLGDLDEPATGDAFETALADYAALFAHKPAAVAVDLHPDYRATRLGEDMAATRSLPL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCHHHHHHCCCH
ERVQHHHAHMAAAMAEAGWRRADGPVVGIALDGLGLGEDGTAWGAEILVCDYRASRRVAR
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHCCHHH
LSPIPLAGGDAASREPWRVLLAHLDRALGRSAVDGDPRLAALLAGKPLLTLRAMMDKGLN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHCCCHHHHHHHHHHCCCC
APLASSAGRLFDAVAAVLGLAPDRLSHEGEAAMALEAAAQGDAPPYPFALHAEAVPIEID
CCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCEEEEECC
PAPLFKALVDDLHAGSPRAAMAAAFHAGLAEAFTGAAVQVAAKEGLTAVALSGGVFQNAR
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHCCCEEEEECCCCHHHHH
LLEETVRRLEAHGLIPLVPGDVPANDGGLAFGQAVVAAARRMG
HHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497190; 9492269 [H]