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The map label for this gene is hyfF [H]

Identifier: 154244127

GI number: 154244127

Start: 165459

End: 166919

Strand: Direct

Name: hyfF [H]

Synonym: Xaut_0169

Alternate gene names: 154244127

Gene position: 165459-166919 (Clockwise)

Preceding gene: 154244126

Following gene: 154244128

Centisome position: 3.12

GC content: 67.62

Gene sequence:

>1461_bases
ATGATCGCCTCCCTCGCCGCGCTGGCGTTCAATCCCGCGCCCGTCATCCTCACCATCCCGGCGGTGGCGGCGGTGCTGCT
GGCGCTGGTTCCGTCCTATCGCGTCTCGGCAGCGCTCAATGTGAGCGCCTGCGCCCTGACGTTCCTCACCGCCCTGGCGC
TGCTGGTGGCGCCGCGCATTGCCGGGCCGTTCCTCATCGTGGACGACCTCAACATCGTGTTCGTGGTGCTGAACACCTTC
GTGGCCTTCACCACCAGCGTGTTCTCGGCGAGCTACATCGGCCACGAGCTGGAGACGGGGCGCCTGACGCCGCTGCACCT
GCGCTTCTACCACGCCATGTACCAGGTGCTGCTGGCGGCCATGAACCTGGCGCTCGTCTCCAACAATCTCGGCGTCATGT
GGGTGGCCATCGAGGTGGCGACCCTCACCACCGTGCTGATGGTGGGGCTTTATCGCACCGAGGCGGCCATCGAGGCGGCA
TGGAAGTATTTCATGCTGGGCTCGGTGGGCATCGCGCTGGCCCTGTTCGGCACCATCCTCGTCTACATGGTGGCCCAGCC
GGTGGTGGGCGAGGGACCGGATGGCATGATCTGGAGCGTGCTGATGAAGCGCGCACGGGATTTCGACCCGGCGCTGCTCA
ACGTCGCCTTCGTGTTCCTGCTGCTCGGCTATGGCACCAAGGTGGGCCTTGCCCCGCTCCATGCCTGGCTGCCCGATGCC
CATTCGGAAGGCCCAACCCCCATCTCGGCGGTGCTGTCGGGGCTGCTGCTCAACGTGGCGCTCTATGCGGTGCTGCGCTT
CAAGCTGCTGCTGGCGGCCCATCCCGGCACGCTGGCGCCGGGGCCGCTGATGATGGCCATGGGCCTCGCCTCGCTGCTGC
TCGCCGGCTTCATGCTGTACCGGCGACGGGACATCAAGCGGCTGTTCGCCTATTCGTCCATCGAGCACATGGGCCTCATC
GCCTTCGCCTTCGGGCTCGGCGGGCCGGTCGCCAATTTCGCCGGTCTGCTGCATATGACCATGCACAGCCTGACCAAGTC
GGCCATCTTCTTCGCGGTGGGCCATGTGTGCCAGGTGAAGGGCACGCAGAAGCTTTCCGATATTCGCGGCCTCACCACCT
CCCATCCGGCGCTGGGCTGGGGCCTCGTGGTGGCGGTGGCGGCTATTGCCGGCCTGCCGCCGGCCGGCGTGTTCATGAGC
GAGTTCCTGCTGGTGACCTCCACCTTCGCGCACCGGCCGTGGCTCGCGCTGCCGCTGGTAGCGGGGCTGCTGCTGGCCTT
CGGCGCACTGATGCTGAAGCTCTCCGGCATCGCCTTCGGCGCGCCCAGCCCGGGGAGCGACAAGGTGAAGGCGAGCCACC
TGCCCATGACGGTGCATCTTGCCCTGGTGCTGGTGGCCGGGCTCTATCTGCCGCCGCCGCTGGTGGCGTGGTTCCGGCAC
GTGGCCGGGCTTCTGGGCTAG

Upstream 100 bases:

>100_bases
TCATCGCCTTCATCGTCATCGGCATCTTCCTGTTCCGCATCCGCGAGCGGTTCGACACCGTGGATGTGGACGCCTTCGAC
CGCTTCCGGGGAGGGCGCGC

Downstream 100 bases:

>100_bases
GGGCGCGCCATGCAAGCCTTCGCACAGCTTCTGGAAAACGGCCGCGCCGGTCCCTGCCGGCCCTTTCCGCGCGCCGAGGT
GAGTGCAGAGCAGTGGACCG

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MIASLAALAFNPAPVILTIPAVAAVLLALVPSYRVSAALNVSACALTFLTALALLVAPRIAGPFLIVDDLNIVFVVLNTF
VAFTTSVFSASYIGHELETGRLTPLHLRFYHAMYQVLLAAMNLALVSNNLGVMWVAIEVATLTTVLMVGLYRTEAAIEAA
WKYFMLGSVGIALALFGTILVYMVAQPVVGEGPDGMIWSVLMKRARDFDPALLNVAFVFLLLGYGTKVGLAPLHAWLPDA
HSEGPTPISAVLSGLLLNVALYAVLRFKLLLAAHPGTLAPGPLMMAMGLASLLLAGFMLYRRRDIKRLFAYSSIEHMGLI
AFAFGLGGPVANFAGLLHMTMHSLTKSAIFFAVGHVCQVKGTQKLSDIRGLTTSHPALGWGLVVAVAAIAGLPPAGVFMS
EFLLVTSTFAHRPWLALPLVAGLLLAFGALMLKLSGIAFGAPSPGSDKVKASHLPMTVHLALVLVAGLYLPPPLVAWFRH
VAGLLG

Sequences:

>Translated_486_residues
MIASLAALAFNPAPVILTIPAVAAVLLALVPSYRVSAALNVSACALTFLTALALLVAPRIAGPFLIVDDLNIVFVVLNTF
VAFTTSVFSASYIGHELETGRLTPLHLRFYHAMYQVLLAAMNLALVSNNLGVMWVAIEVATLTTVLMVGLYRTEAAIEAA
WKYFMLGSVGIALALFGTILVYMVAQPVVGEGPDGMIWSVLMKRARDFDPALLNVAFVFLLLGYGTKVGLAPLHAWLPDA
HSEGPTPISAVLSGLLLNVALYAVLRFKLLLAAHPGTLAPGPLMMAMGLASLLLAGFMLYRRRDIKRLFAYSSIEHMGLI
AFAFGLGGPVANFAGLLHMTMHSLTKSAIFFAVGHVCQVKGTQKLSDIRGLTTSHPALGWGLVVAVAAIAGLPPAGVFMS
EFLLVTSTFAHRPWLALPLVAGLLLAFGALMLKLSGIAFGAPSPGSDKVKASHLPMTVHLALVLVAGLYLPPPLVAWFRH
VAGLLG
>Mature_486_residues
MIASLAALAFNPAPVILTIPAVAAVLLALVPSYRVSAALNVSACALTFLTALALLVAPRIAGPFLIVDDLNIVFVVLNTF
VAFTTSVFSASYIGHELETGRLTPLHLRFYHAMYQVLLAAMNLALVSNNLGVMWVAIEVATLTTVLMVGLYRTEAAIEAA
WKYFMLGSVGIALALFGTILVYMVAQPVVGEGPDGMIWSVLMKRARDFDPALLNVAFVFLLLGYGTKVGLAPLHAWLPDA
HSEGPTPISAVLSGLLLNVALYAVLRFKLLLAAHPGTLAPGPLMMAMGLASLLLAGFMLYRRRDIKRLFAYSSIEHMGLI
AFAFGLGGPVANFAGLLHMTMHSLTKSAIFFAVGHVCQVKGTQKLSDIRGLTTSHPALGWGLVVAVAAIAGLPPAGVFMS
EFLLVTSTFAHRPWLALPLVAGLLLAFGALMLKLSGIAFGAPSPGSDKVKASHLPMTVHLALVLVAGLYLPPPLVAWFRH
VAGLLG

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=197, Percent_Identity=28.4263959390863, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI251831117, Length=388, Percent_Identity=25, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788831, Length=419, Percent_Identity=38.9021479713604, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1788827, Length=376, Percent_Identity=27.6595744680851, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788613, Length=211, Percent_Identity=33.6492890995261, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788829, Length=433, Percent_Identity=26.5588914549654, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788614, Length=434, Percent_Identity=27.8801843317972, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI145693160, Length=384, Percent_Identity=27.0833333333333, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI2367154, Length=386, Percent_Identity=27.4611398963731, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51387; Mature: 51387

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIASLAALAFNPAPVILTIPAVAAVLLALVPSYRVSAALNVSACALTFLTALALLVAPRI
CCCHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AGPFLIVDDLNIVFVVLNTFVAFTTSVFSASYIGHELETGRLTPLHLRFYHAMYQVLLAA
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
MNLALVSNNLGVMWVAIEVATLTTVLMVGLYRTEAAIEAAWKYFMLGSVGIALALFGTIL
HHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYMVAQPVVGEGPDGMIWSVLMKRARDFDPALLNVAFVFLLLGYGTKVGLAPLHAWLPDA
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
HSEGPTPISAVLSGLLLNVALYAVLRFKLLLAAHPGTLAPGPLMMAMGLASLLLAGFMLY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RRRDIKRLFAYSSIEHMGLIAFAFGLGGPVANFAGLLHMTMHSLTKSAIFFAVGHVCQVK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTQKLSDIRGLTTSHPALGWGLVVAVAAIAGLPPAGVFMSEFLLVTSTFAHRPWLALPLV
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
AGLLLAFGALMLKLSGIAFGAPSPGSDKVKASHLPMTVHLALVLVAGLYLPPPLVAWFRH
HHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
VAGLLG
HHHHCC
>Mature Secondary Structure
MIASLAALAFNPAPVILTIPAVAAVLLALVPSYRVSAALNVSACALTFLTALALLVAPRI
CCCHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AGPFLIVDDLNIVFVVLNTFVAFTTSVFSASYIGHELETGRLTPLHLRFYHAMYQVLLAA
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
MNLALVSNNLGVMWVAIEVATLTTVLMVGLYRTEAAIEAAWKYFMLGSVGIALALFGTIL
HHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYMVAQPVVGEGPDGMIWSVLMKRARDFDPALLNVAFVFLLLGYGTKVGLAPLHAWLPDA
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
HSEGPTPISAVLSGLLLNVALYAVLRFKLLLAAHPGTLAPGPLMMAMGLASLLLAGFMLY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RRRDIKRLFAYSSIEHMGLIAFAFGLGGPVANFAGLLHMTMHSLTKSAIFFAVGHVCQVK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTQKLSDIRGLTTSHPALGWGLVVAVAAIAGLPPAGVFMSEFLLVTSTFAHRPWLALPLV
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
AGLLLAFGALMLKLSGIAFGAPSPGSDKVKASHLPMTVHLALVLVAGLYLPPPLVAWFRH
HHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
VAGLLG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA