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The map label for this gene is hyfE [C]

Identifier: 154244126

GI number: 154244126

Start: 164782

End: 165462

Strand: Direct

Name: hyfE [C]

Synonym: Xaut_0168

Alternate gene names: 154244126

Gene position: 164782-165462 (Clockwise)

Preceding gene: 154244125

Following gene: 154244127

Centisome position: 3.1

GC content: 66.81

Gene sequence:

>681_bases
ATGATCGAGCACGCCATCACCCCCAATCTCGTCTTCGACGTGTCCCACATGTTCGCGGGCGGGCTGGTGCTCATCTCCTT
CCTGATGCTCTACCAGGACCGGCTTTACGCGCTGCTGAACGTGTTCGCCCTGCAGGCGGTGGCTCTGGCGCTGGCGGTGG
CGTGGCAGGCCTACGCCCAGGACGCGCCGCATCTCTACGTCACGGCAGGCATCGCCCTGGTGTTCAAGGCGCTGGTGATC
CCGGTGGCGCTGCATCGCATGGTGGCGCGGCTCGGCATCCATCGGCAGGTGGAGACGGTGGTGTCCATCGGCCCCACCAT
GCTGGCCGGCATCGCCCTCGTCGCCTTGTCCCTGGTGGTGATGCTGAAGGCTACCCCCGCCGCCGACGCGCTGGCGCGGG
AAGACCTCGCCTTCGCACTCTCGGTGGTGCTGCTGGGCCTCTTGATGATGGTGACGCGGCGCAATGCCGTGAGCCAGGTG
GTGGGCTTCATGTCGCTGGAGAACGGCCTGATCCTCGCCGCCACGGGGGCCAAGGGCATGCCGCTGGTGGTGGAGATCTC
GGTGGCCTTCTCCGTGCTCATCGCCTTCATCGTCATCGGCATCTTCCTGTTCCGCATCCGCGAGCGGTTCGACACCGTGG
ATGTGGACGCCTTCGACCGCTTCCGGGGAGGGCGCGCATGA

Upstream 100 bases:

>100_bases
TCGCCAAGATGCGGGTGTTCCGCGTGCCGGACTTCCTCGGCGCGGCGCTCATGCTCGGGCTGTTGGGAACGCTGCTGCTG
TTCGTGTCGGGGATCCTGTG

Downstream 100 bases:

>100_bases
TCGCCTCCCTCGCCGCGCTGGCGTTCAATCCCGCGCCCGTCATCCTCACCATCCCGGCGGTGGCGGCGGTGCTGCTGGCG
CTGGTTCCGTCCTATCGCGT

Product: putative hydrogenase-4 component E

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MIEHAITPNLVFDVSHMFAGGLVLISFLMLYQDRLYALLNVFALQAVALALAVAWQAYAQDAPHLYVTAGIALVFKALVI
PVALHRMVARLGIHRQVETVVSIGPTMLAGIALVALSLVVMLKATPAADALAREDLAFALSVVLLGLLMMVTRRNAVSQV
VGFMSLENGLILAATGAKGMPLVVEISVAFSVLIAFIVIGIFLFRIRERFDTVDVDAFDRFRGGRA

Sequences:

>Translated_226_residues
MIEHAITPNLVFDVSHMFAGGLVLISFLMLYQDRLYALLNVFALQAVALALAVAWQAYAQDAPHLYVTAGIALVFKALVI
PVALHRMVARLGIHRQVETVVSIGPTMLAGIALVALSLVVMLKATPAADALAREDLAFALSVVLLGLLMMVTRRNAVSQV
VGFMSLENGLILAATGAKGMPLVVEISVAFSVLIAFIVIGIFLFRIRERFDTVDVDAFDRFRGGRA
>Mature_226_residues
MIEHAITPNLVFDVSHMFAGGLVLISFLMLYQDRLYALLNVFALQAVALALAVAWQAYAQDAPHLYVTAGIALVFKALVI
PVALHRMVARLGIHRQVETVVSIGPTMLAGIALVALSLVVMLKATPAADALAREDLAFALSVVLLGLLMMVTRRNAVSQV
VGFMSLENGLILAATGAKGMPLVVEISVAFSVLIAFIVIGIFLFRIRERFDTVDVDAFDRFRGGRA

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24347; Mature: 24347

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEHAITPNLVFDVSHMFAGGLVLISFLMLYQDRLYALLNVFALQAVALALAVAWQAYAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DAPHLYVTAGIALVFKALVIPVALHRMVARLGIHRQVETVVSIGPTMLAGIALVALSLVV
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLKATPAADALAREDLAFALSVVLLGLLMMVTRRNAVSQVVGFMSLENGLILAATGAKGM
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
PLVVEISVAFSVLIAFIVIGIFLFRIRERFDTVDVDAFDRFRGGRA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
>Mature Secondary Structure
MIEHAITPNLVFDVSHMFAGGLVLISFLMLYQDRLYALLNVFALQAVALALAVAWQAYAQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DAPHLYVTAGIALVFKALVIPVALHRMVARLGIHRQVETVVSIGPTMLAGIALVALSLVV
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLKATPAADALAREDLAFALSVVLLGLLMMVTRRNAVSQVVGFMSLENGLILAATGAKGM
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
PLVVEISVAFSVLIAFIVIGIFLFRIRERFDTVDVDAFDRFRGGRA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]