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The map label for this gene is nifE [H]

Identifier: 154244049

GI number: 154244049

Start: 100648

End: 102549

Strand: Direct

Name: nifE [H]

Synonym: Xaut_0091

Alternate gene names: 154244049

Gene position: 100648-102549 (Clockwise)

Preceding gene: 154244048

Following gene: 154244050

Centisome position: 1.9

GC content: 64.77

Gene sequence:

>1902_bases
ATGAGCTCGGTTTCCGCTACGATCCAGGAAGTCTTCAACGAGCCAAGCTGCGCGAAGAACCAGAACAAATCGGAAGCGGA
AAAGAAGAAGGGGTGCACCAAGCAGCTCCAGCCCGGCGGTGCCGCCGGCGGCTGCGCCTTCGACGGCGCCAAGATCGCCC
TCCAGCCCCTGACCGACGTCGCCCATCTCGTGCACGGCCCGATCGCCTGCGAGGGCAACAGCTGGGACAATCGCGGCGCG
AAGTCGTCCGGCTCGCAGATCTGGCGGACGGGCTTCACCACGGACATCAACGAGACCGACGTGGTGTTCGGCGGTGAGAA
GCGCCTGTTCAAGTCGATCAAGGAAATCATCGAGAAGTACAATCCCCCCGCCGTCTTCGTCTACCAGACCTGCGTGCCCG
CCATGATCGGCGACGACATCGACGCGGTCTGCAAGGCGGCAAAGGAGAAGTTCGGCACGCCGGTGATCCCGGTGAACTCG
CCCGGCTTCGTGGGTCCGAAGAACCTCGGCAACAAGCTCGCGGGCGAAGCTATCCTCCAGCACGTCATCGGCACCGAGGA
GCCGGAATACACCACGCCCTACGACCTGAATATCATCGGCGAATACAATCTGTCCGGTGAATTGTGGCAGGTGAAGCCGT
TGCTCGACGAGCTCGGCATCCGCATCATGGCCTGCATCTCCGGCGACGGGCGCTACAAGGATGTGGCCTCCTCGCACCGC
GCCAAGGCGGCGATGATGGTGTGTTCCAAGGCCATGATCAACGTGGCCCGCAAGATGGAGGAGCGCTACGACATCCCCTT
CTTCGAGGGCTCCTTTTACGGCATCCAGGACTCGTCCGACTCGCTCCGCGAGATTGCCCGCATGCTCATCGAGCGCGGCG
CCGATCCCGAGCTGATGGACCGCACCGAGGCCCTCATCGAGCGCGAGGAGGCGAACGCCTGGGCCGCCATCGCCAAGTAC
AAGCCCCGCTTCAAGGACAAGAAGGTGCTGCTGATCACCGGCGGCGTGAAGTCCTGGTCGGTGGTGGCGGCGTTGCAGGA
AGCCGGCCTCGAGCTGGTGGGCACTTCCGTTAAGAAGTCCACCCGCGAGGACAAGGAGCGCATCAAGGAGCTCATGGGGC
AGGACGCCCACATGATCGACGACATGACGCCCCGTGAAATGTACAAGATGCTGAAGGACGCGAAGGCCGACATCATGCTC
TCGGGCGGCCGCTCGCAGTTCATCGCACTCAAGGCCGCCATGCCGTGGCTCGACATCAACCAGGAGCGCCACCACGCCTA
CATGGGCTATGTGGGCATGGTGAAGCTGGTCGAGGAGATCGACAAGGCGCTCTACAATCCCATCTGGGAGCAGGTGCGCA
AACCCGCCCCGTGGGACAATCCGGCCAACAACTGGCAGAACCGCGCCATCGCCGAGATGGAGGCGGAAGCCGCCGCGCTC
GCCGCCGATCCGGTGGCCGCCGAGAAGGCGCGCCGGGCCAAGAAGATCTGCAACTGCAAGAGTGTCGACCTCGGTACCAT
CGAGGACGCCATCGCCGCCCACGGCCTCACCACCGTGGAAGGCGTGAAGACCCACACCAATGCCTCCGGCGGCTGCGGCG
CATGCGCCGGTCGCATCGAGGAGATCTTCGAGGCCCGTGGCCTGGTGCCGGCGGAAGAGCCGGAGCTTGATCCGGTCGCG
GCGGAGGCCAAGCGGCGCGCCAAGAAGGTGTGCAATTGCATGAGCGTGGATGTAGGCACCATCGAGGATGCCATCCACGC
CGGCGCTGCCAACCTCGCCGACGTGAAGGCGCAGACCAAGGCCACCGGCGGCTGCGGCGGCTGCGCCGAGCGCATCGAGG
AGATGCTGGACGCGGCTGTTGCGGATGCCGACGTGCCGGTCACCCTCGTGGCAGCGGAATAG

Upstream 100 bases:

>100_bases
TCGATCCGGCCCGGGGCCCCGCCCGGGCCGGATCGAACAGGAGCCTGCCCTTTTCGGAAGTTCTGATCTTTACACAGCCC
GCTTCTCGAAGGAGAGATCC

Downstream 100 bases:

>100_bases
GAGGCCGGCATGGCGAAATTCGTCACGGGCAAGAAGGCCTGCGCGGTCAATCCGCTGAAGATGAGCCAGCCCATCGGCGG
CGCGCTCGCCTTCATGGGGC

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MSSVSATIQEVFNEPSCAKNQNKSEAEKKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDVAHLVHGPIACEGNSWDNRGA
KSSGSQIWRTGFTTDINETDVVFGGEKRLFKSIKEIIEKYNPPAVFVYQTCVPAMIGDDIDAVCKAAKEKFGTPVIPVNS
PGFVGPKNLGNKLAGEAILQHVIGTEEPEYTTPYDLNIIGEYNLSGELWQVKPLLDELGIRIMACISGDGRYKDVASSHR
AKAAMMVCSKAMINVARKMEERYDIPFFEGSFYGIQDSSDSLREIARMLIERGADPELMDRTEALIEREEANAWAAIAKY
KPRFKDKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKSTREDKERIKELMGQDAHMIDDMTPREMYKMLKDAKADIML
SGGRSQFIALKAAMPWLDINQERHHAYMGYVGMVKLVEEIDKALYNPIWEQVRKPAPWDNPANNWQNRAIAEMEAEAAAL
AADPVAAEKARRAKKICNCKSVDLGTIEDAIAAHGLTTVEGVKTHTNASGGCGACAGRIEEIFEARGLVPAEEPELDPVA
AEAKRRAKKVCNCMSVDVGTIEDAIHAGAANLADVKAQTKATGGCGGCAERIEEMLDAAVADADVPVTLVAAE

Sequences:

>Translated_633_residues
MSSVSATIQEVFNEPSCAKNQNKSEAEKKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDVAHLVHGPIACEGNSWDNRGA
KSSGSQIWRTGFTTDINETDVVFGGEKRLFKSIKEIIEKYNPPAVFVYQTCVPAMIGDDIDAVCKAAKEKFGTPVIPVNS
PGFVGPKNLGNKLAGEAILQHVIGTEEPEYTTPYDLNIIGEYNLSGELWQVKPLLDELGIRIMACISGDGRYKDVASSHR
AKAAMMVCSKAMINVARKMEERYDIPFFEGSFYGIQDSSDSLREIARMLIERGADPELMDRTEALIEREEANAWAAIAKY
KPRFKDKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKSTREDKERIKELMGQDAHMIDDMTPREMYKMLKDAKADIML
SGGRSQFIALKAAMPWLDINQERHHAYMGYVGMVKLVEEIDKALYNPIWEQVRKPAPWDNPANNWQNRAIAEMEAEAAAL
AADPVAAEKARRAKKICNCKSVDLGTIEDAIAAHGLTTVEGVKTHTNASGGCGACAGRIEEIFEARGLVPAEEPELDPVA
AEAKRRAKKVCNCMSVDVGTIEDAIHAGAANLADVKAQTKATGGCGGCAERIEEMLDAAVADADVPVTLVAAE
>Mature_632_residues
SSVSATIQEVFNEPSCAKNQNKSEAEKKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDVAHLVHGPIACEGNSWDNRGAK
SSGSQIWRTGFTTDINETDVVFGGEKRLFKSIKEIIEKYNPPAVFVYQTCVPAMIGDDIDAVCKAAKEKFGTPVIPVNSP
GFVGPKNLGNKLAGEAILQHVIGTEEPEYTTPYDLNIIGEYNLSGELWQVKPLLDELGIRIMACISGDGRYKDVASSHRA
KAAMMVCSKAMINVARKMEERYDIPFFEGSFYGIQDSSDSLREIARMLIERGADPELMDRTEALIEREEANAWAAIAKYK
PRFKDKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKSTREDKERIKELMGQDAHMIDDMTPREMYKMLKDAKADIMLS
GGRSQFIALKAAMPWLDINQERHHAYMGYVGMVKLVEEIDKALYNPIWEQVRKPAPWDNPANNWQNRAIAEMEAEAAALA
ADPVAAEKARRAKKICNCKSVDLGTIEDAIAAHGLTTVEGVKTHTNASGGCGACAGRIEEIFEARGLVPAEEPELDPVAA
EAKRRAKKVCNCMSVDVGTIEDAIHAGAANLADVKAQTKATGGCGGCAERIEEMLDAAVADADVPVTLVAAE

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF00148 Oxidored_nitro [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 68553; Mature: 68422

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVSATIQEVFNEPSCAKNQNKSEAEKKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDV
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEHHHHHHH
AHLVHGPIACEGNSWDNRGAKSSGSQIWRTGFTTDINETDVVFGGEKRLFKSIKEIIEKY
HHHHCCCEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
NPPAVFVYQTCVPAMIGDDIDAVCKAAKEKFGTPVIPVNSPGFVGPKNLGNKLAGEAILQ
CCCEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHH
HVIGTEEPEYTTPYDLNIIGEYNLSGELWQVKPLLDELGIRIMACISGDGRYKDVASSHR
HHHCCCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
AKAAMMVCSKAMINVARKMEERYDIPFFEGSFYGIQDSSDSLREIARMLIERGADPELMD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
RTEALIEREEANAWAAIAKYKPRFKDKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKS
HHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHH
TREDKERIKELMGQDAHMIDDMTPREMYKMLKDAKADIMLSGGRSQFIALKAAMPWLDIN
HHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCC
QERHHAYMGYVGMVKLVEEIDKALYNPIWEQVRKPAPWDNPANNWQNRAIAEMEAEAAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AADPVAAEKARRAKKICNCKSVDLGTIEDAIAAHGLTTVEGVKTHTNASGGCGACAGRIE
HCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHCCHHCCCCCCCHHHHHHHHH
EIFEARGLVPAEEPELDPVAAEAKRRAKKVCNCMSVDVGTIEDAIHAGAANLADVKAQTK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHC
ATGGCGGCAERIEEMLDAAVADADVPVTLVAAE
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
SSVSATIQEVFNEPSCAKNQNKSEAEKKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDV
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEHHHHHHH
AHLVHGPIACEGNSWDNRGAKSSGSQIWRTGFTTDINETDVVFGGEKRLFKSIKEIIEKY
HHHHCCCEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
NPPAVFVYQTCVPAMIGDDIDAVCKAAKEKFGTPVIPVNSPGFVGPKNLGNKLAGEAILQ
CCCEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHH
HVIGTEEPEYTTPYDLNIIGEYNLSGELWQVKPLLDELGIRIMACISGDGRYKDVASSHR
HHHCCCCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
AKAAMMVCSKAMINVARKMEERYDIPFFEGSFYGIQDSSDSLREIARMLIERGADPELMD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHH
RTEALIEREEANAWAAIAKYKPRFKDKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKS
HHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHH
TREDKERIKELMGQDAHMIDDMTPREMYKMLKDAKADIMLSGGRSQFIALKAAMPWLDIN
HHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCC
QERHHAYMGYVGMVKLVEEIDKALYNPIWEQVRKPAPWDNPANNWQNRAIAEMEAEAAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AADPVAAEKARRAKKICNCKSVDLGTIEDAIAAHGLTTVEGVKTHTNASGGCGACAGRIE
HCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHCCHHCCCCCCCHHHHHHHHH
EIFEARGLVPAEEPELDPVAAEAKRRAKKVCNCMSVDVGTIEDAIHAGAANLADVKAQTK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHC
ATGGCGGCAERIEEMLDAAVADADVPVTLVAAE
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]