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The map label for this gene is nifN [H]

Identifier: 154244050

GI number: 154244050

Start: 102559

End: 103986

Strand: Direct

Name: nifN [H]

Synonym: Xaut_0092

Alternate gene names: 154244050

Gene position: 102559-103986 (Clockwise)

Preceding gene: 154244049

Following gene: 154244051

Centisome position: 1.93

GC content: 64.92

Gene sequence:

>1428_bases
ATGGCGAAATTCGTCACGGGCAAGAAGGCCTGCGCGGTCAATCCGCTGAAGATGAGCCAGCCCATCGGCGGCGCGCTCGC
CTTCATGGGGCTGCGCGGTGCCATGCCACTGCTGCACGGCTCGCAGGGCTGCACGTCGTTCGGCCTGGTGCTGTTCGTGC
GCCACTTCAAGGAGGCGATCCCGCTCCAGACCACTGCCATGAGTGAGGTCGCGACCGTGCTCGGCGGCCTCGACAATGTG
GAGCAGGCGGTTCTCAACATCTACAACCGGACCAAGCCGGAGATCATCGGCATCTGCTCCACCGGCGTCACCGAGACCAA
GGGCGACGACGTGGACGGCTATATCAAGCTGATCCGTGCCAAGTATCCGCAGCTTGCCGACTTCCCGATGGTCTATGTCT
CCACGCCCGACTTCAAGGACGCCTTCCAGGACGGCTGGGAGAAGACGGTCGCGAAGATGGTCGAGGTGCTGGTGAAGACG
CCGGAGCCGGAGACCAAGCGCGATCCCAAGCGCATCAATGTGCTGCCGGGTTGCCACCTGCAGCCCGGCGACCTCGACGA
ACTGCGCACCATCTTCGAGGATTTCGGCCTGGAGCCATCCTTCCTGCCGGATCTCGCCGGCTCGCTGGACGGGCACATTC
CGGACGACTTTACCCCCACCACCATTGGCGGGATCGGGGTGGAGGAAATCGCCACCATGGGCGATGCCTCCTGGACCATC
GCCATCGGCGCGCAGATGCGCCGGGCGGCCGAGGCCATGGAGAAGAAGACCGGTGTGCCCTATCGGCTGTTCGAGCGGCT
GTGCGGGCTCGGTCCGAACGATGAATTCTTCATGTTCCTGAGCGAGATCTCGGGACGCGAGGTGCCCAAGAAGTATCGCC
GGCAGCGGGGCCAGCTGGTGGATGCCATGCTGGACGCCCACTTCCATCTGGGCGGCCGCAAGCTCGCCATTGGTGCCGAG
CCGGACCTCCTGTTCGACGTGGCGTCCATGCTCCACGAGATGGGCACCCATATCACCACAGCGGTCACCACCACCCAGTC
GCCGGTGTTCGAGCAGCTGGAGGCGGATGAGGTGCTGCTCGGCGATCTCGAGGACCTGGAGACGCTGGCGAAGGAACGCG
GTTGCGACCTGCTGCTGACCCATTCCCATGGCCGGCAGGCGGCGGAGCGGCTGAACATTCCGTTCCACCGCATCGGCATT
CCCATGTTTGACCGCATCGGCGCCGGCCACCGCATGATCGTGGGCTATCGCGGCACGCGCGAGCTGATCTTCACGCTGGC
GAACCTGTGCATCGCTGATCACGAGGCGAACCACGACGTAACGCCTGACACCTGGAAAAATCCGTGGGACGGGGATGGTC
ACGGCCACGCCCATGCCCCAGCGGTGACGCCCACCCACGGCACCGCCGTGAGCACGTCCCACCACTAA

Upstream 100 bases:

>100_bases
CTGCGGCGGCTGCGCCGAGCGCATCGAGGAGATGCTGGACGCGGCTGTTGCGGATGCCGACGTGCCGGTCACCCTCGTGG
CAGCGGAATAGGAGGCCGGC

Downstream 100 bases:

>100_bases
GAACGAGCAGAGGCGCACCCTCCCATGAAAGTCGCATTTGCAACCCAGGACCTCAAACGGGTCGATGCCCATTTCGGGTG
GGCCAAGAACATCGCCATCT

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MAKFVTGKKACAVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAIPLQTTAMSEVATVLGGLDNV
EQAVLNIYNRTKPEIIGICSTGVTETKGDDVDGYIKLIRAKYPQLADFPMVYVSTPDFKDAFQDGWEKTVAKMVEVLVKT
PEPETKRDPKRINVLPGCHLQPGDLDELRTIFEDFGLEPSFLPDLAGSLDGHIPDDFTPTTIGGIGVEEIATMGDASWTI
AIGAQMRRAAEAMEKKTGVPYRLFERLCGLGPNDEFFMFLSEISGREVPKKYRRQRGQLVDAMLDAHFHLGGRKLAIGAE
PDLLFDVASMLHEMGTHITTAVTTTQSPVFEQLEADEVLLGDLEDLETLAKERGCDLLLTHSHGRQAAERLNIPFHRIGI
PMFDRIGAGHRMIVGYRGTRELIFTLANLCIADHEANHDVTPDTWKNPWDGDGHGHAHAPAVTPTHGTAVSTSHH

Sequences:

>Translated_475_residues
MAKFVTGKKACAVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAIPLQTTAMSEVATVLGGLDNV
EQAVLNIYNRTKPEIIGICSTGVTETKGDDVDGYIKLIRAKYPQLADFPMVYVSTPDFKDAFQDGWEKTVAKMVEVLVKT
PEPETKRDPKRINVLPGCHLQPGDLDELRTIFEDFGLEPSFLPDLAGSLDGHIPDDFTPTTIGGIGVEEIATMGDASWTI
AIGAQMRRAAEAMEKKTGVPYRLFERLCGLGPNDEFFMFLSEISGREVPKKYRRQRGQLVDAMLDAHFHLGGRKLAIGAE
PDLLFDVASMLHEMGTHITTAVTTTQSPVFEQLEADEVLLGDLEDLETLAKERGCDLLLTHSHGRQAAERLNIPFHRIGI
PMFDRIGAGHRMIVGYRGTRELIFTLANLCIADHEANHDVTPDTWKNPWDGDGHGHAHAPAVTPTHGTAVSTSHH
>Mature_474_residues
AKFVTGKKACAVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAIPLQTTAMSEVATVLGGLDNVE
QAVLNIYNRTKPEIIGICSTGVTETKGDDVDGYIKLIRAKYPQLADFPMVYVSTPDFKDAFQDGWEKTVAKMVEVLVKTP
EPETKRDPKRINVLPGCHLQPGDLDELRTIFEDFGLEPSFLPDLAGSLDGHIPDDFTPTTIGGIGVEEIATMGDASWTIA
IGAQMRRAAEAMEKKTGVPYRLFERLCGLGPNDEFFMFLSEISGREVPKKYRRQRGQLVDAMLDAHFHLGGRKLAIGAEP
DLLFDVASMLHEMGTHITTAVTTTQSPVFEQLEADEVLLGDLEDLETLAKERGCDLLLTHSHGRQAAERLNIPFHRIGIP
MFDRIGAGHRMIVGYRGTRELIFTLANLCIADHEANHDVTPDTWKNPWDGDGHGHAHAPAVTPTHGTAVSTSHH

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 52010; Mature: 51879

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFVTGKKACAVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAI
CCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHC
PLQTTAMSEVATVLGGLDNVEQAVLNIYNRTKPEIIGICSTGVTETKGDDVDGYIKLIRA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KYPQLADFPMVYVSTPDFKDAFQDGWEKTVAKMVEVLVKTPEPETKRDPKRINVLPGCHL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCC
QPGDLDELRTIFEDFGLEPSFLPDLAGSLDGHIPDDFTPTTIGGIGVEEIATMGDASWTI
CCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEE
AIGAQMRRAAEAMEKKTGVPYRLFERLCGLGPNDEFFMFLSEISGREVPKKYRRQRGQLV
EECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
DAMLDAHFHLGGRKLAIGAEPDLLFDVASMLHEMGTHITTAVTTTQSPVFEQLEADEVLL
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCHHHH
GDLEDLETLAKERGCDLLLTHSHGRQAAERLNIPFHRIGIPMFDRIGAGHRMIVGYRGTR
CCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCHHHHCCCHHHHCCCCCEEEEEECCHH
ELIFTLANLCIADHEANHDVTPDTWKNPWDGDGHGHAHAPAVTPTHGTAVSTSHH
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKFVTGKKACAVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAI
CCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHC
PLQTTAMSEVATVLGGLDNVEQAVLNIYNRTKPEIIGICSTGVTETKGDDVDGYIKLIRA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KYPQLADFPMVYVSTPDFKDAFQDGWEKTVAKMVEVLVKTPEPETKRDPKRINVLPGCHL
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCC
QPGDLDELRTIFEDFGLEPSFLPDLAGSLDGHIPDDFTPTTIGGIGVEEIATMGDASWTI
CCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEE
AIGAQMRRAAEAMEKKTGVPYRLFERLCGLGPNDEFFMFLSEISGREVPKKYRRQRGQLV
EECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
DAMLDAHFHLGGRKLAIGAEPDLLFDVASMLHEMGTHITTAVTTTQSPVFEQLEADEVLL
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCHHHH
GDLEDLETLAKERGCDLLLTHSHGRQAAERLNIPFHRIGIPMFDRIGAGHRMIVGYRGTR
CCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCHHHHCCCHHHHCCCCCEEEEEECCHH
ELIFTLANLCIADHEANHDVTPDTWKNPWDGDGHGHAHAPAVTPTHGTAVSTSHH
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]