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The map label for this gene is nifK [H]

Identifier: 154244048

GI number: 154244048

Start: 98968

End: 100527

Strand: Direct

Name: nifK [H]

Synonym: Xaut_0090

Alternate gene names: 154244048

Gene position: 98968-100527 (Clockwise)

Preceding gene: 154244047

Following gene: 154244049

Centisome position: 1.86

GC content: 62.24

Gene sequence:

>1560_bases
ATGCCACAGAATGCAGATAACGTGCTCGATCACTTCGAGCTGTTCCGCGGTCCCGAGTACCAGCAGATGCTGGCCAACAA
GAAGAAGCTGTTCGAGAACCCCCGCGACCCCGCCGAAGTCGAGCGCGTCCGCGAGTGGGCCAAGACTCCCGAGTACAAGG
AAAAGAACTTCGCCCGCGAAGCTCTGACCGTGAACCCGGCCAAGGCCTGCCAGCCGCTCGGCGCCGTCTTCGCCGCCGTG
GGCTTCGAGCAGACCATCCCCTTCGTGCACGGCTCGCAGGGTTGCGTCGCTTACTATCGGTCGCACTTCTCGCGTCACTT
CAAGGAGCCCAGCTCCTGCGTGTCCTCTTCCATGACGGAAGACGCGGCGGTGTTCGGCGGCCTGAACAACATGATCGACG
GCCTCGCCAACACCTACTCCATGTACAAGCCGAAGATGATTTCGGTCTCCACCACCTGCATGGCGGAAGTCATCGGTGAC
GACCTGAACGCCTTCATCAAGACCGCCAAGGAAAAGGGCTCGGTTCCCCAGGAATACGACGTTCCCTTCGCCCACACCCC
GGCGTTCGTCGGCAGCCACATCACCGGCTACGACAACGTGCTGAAGGGCATCTTCGAGCACTTCTGGGACGGCAAGGCCG
GCACCGCGCCGAAGCTCGAGCGCGTCCCGAACGAGAAGATCAACTTCATCGGCGGCTTCGACGGCTACACCGTCGGCAAC
CTGCGTGAAATCAAGCGCCTGTTCGGCCTGATGGGCGTGGAATACACCATCCTCGCCGACAATTCCGAAGTGTGGGACAC
CCCCACCGACGGCGAATTCCGCATGTATGACGGCGGCACGACCCTGGAAGAGGCGGCCGACGCGCTGCACGCCAAGGCCA
CCATCTCCATGCAGGAGTTCTGCACCGAGAAGACCCTGCCGCTGATCGCGAACCACGGCCAGGAAGTGGTTGCCTTCAAC
CACCCGGTGGGCGTGAAGGGCACCGACGAGTTCCTGATGGCGATCTCCCGCATCTCCGGCAAGGAGATTCCGGAAGCCCT
CGCCAAGGAGCGCGGTCGTCTGGTTGACGCCATCGCCGACTCCAACGCCCACATCCACGGCAAGAAGTTCGCCATCTACG
GCGATCCCGACCTCTGCATGGGCCTGGCGGCCTTCCTGCTCGAGCTCGGTGCCGAGCCGACCCACGTGCTTGCCACCAAC
GGCAACAAGAAGTGGGCCGACAAGATGCAGGAGCTGTTCGACTCTTCGCCGTTCGGCCAGAATTGCCAAGTCTATCCCGG
CCGTGACCTGTGGCACATGCGTTCGCTGCTCTTCACCGAGCCGGTGGACTTCCTGATCGGCAACACCTACGGCAAGTATC
TCGAGCGCGACACGGGCACCCCGCTGATCCGCATCGGCTTCCCGGTGTTCGACCGTCACCACCATCACCGCCGTCCGGTG
TGGGGTTATCAGGGCGGCATGAACGTGCTGATCACCATCCTCGACAAGATCTTCGACACCATCGATCAGAACACGATCGT
CCCGGCGAAGACCGACTACTCGTTCGACATCATCCGTTGA

Upstream 100 bases:

>100_bases
CGCGGCCGAGTGACCGCCTGAGCCCGAAACGCCCCTCCCCTTTTTGGGGAGGGGCACCTGGCGGGCTTGATCCGTTTTGA
CCCACCCATAGGGGTTCGAG

Downstream 100 bases:

>100_bases
TCGCAGAAGTTTGACGATAATCGATCCGGCCCGGGGCCCCGCCCGGGCCGGATCGAACAGGAGCCTGCCCTTTTCGGAAG
TTCTGATCTTTACACAGCCC

Product: nitrogenase molybdenum-iron protein subunit beta

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MPQNADNVLDHFELFRGPEYQQMLANKKKLFENPRDPAEVERVREWAKTPEYKEKNFAREALTVNPAKACQPLGAVFAAV
GFEQTIPFVHGSQGCVAYYRSHFSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANTYSMYKPKMISVSTTCMAEVIGD
DLNAFIKTAKEKGSVPQEYDVPFAHTPAFVGSHITGYDNVLKGIFEHFWDGKAGTAPKLERVPNEKINFIGGFDGYTVGN
LREIKRLFGLMGVEYTILADNSEVWDTPTDGEFRMYDGGTTLEEAADALHAKATISMQEFCTEKTLPLIANHGQEVVAFN
HPVGVKGTDEFLMAISRISGKEIPEALAKERGRLVDAIADSNAHIHGKKFAIYGDPDLCMGLAAFLLELGAEPTHVLATN
GNKKWADKMQELFDSSPFGQNCQVYPGRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPVFDRHHHHRRPV
WGYQGGMNVLITILDKIFDTIDQNTIVPAKTDYSFDIIR

Sequences:

>Translated_519_residues
MPQNADNVLDHFELFRGPEYQQMLANKKKLFENPRDPAEVERVREWAKTPEYKEKNFAREALTVNPAKACQPLGAVFAAV
GFEQTIPFVHGSQGCVAYYRSHFSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANTYSMYKPKMISVSTTCMAEVIGD
DLNAFIKTAKEKGSVPQEYDVPFAHTPAFVGSHITGYDNVLKGIFEHFWDGKAGTAPKLERVPNEKINFIGGFDGYTVGN
LREIKRLFGLMGVEYTILADNSEVWDTPTDGEFRMYDGGTTLEEAADALHAKATISMQEFCTEKTLPLIANHGQEVVAFN
HPVGVKGTDEFLMAISRISGKEIPEALAKERGRLVDAIADSNAHIHGKKFAIYGDPDLCMGLAAFLLELGAEPTHVLATN
GNKKWADKMQELFDSSPFGQNCQVYPGRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPVFDRHHHHRRPV
WGYQGGMNVLITILDKIFDTIDQNTIVPAKTDYSFDIIR
>Mature_518_residues
PQNADNVLDHFELFRGPEYQQMLANKKKLFENPRDPAEVERVREWAKTPEYKEKNFAREALTVNPAKACQPLGAVFAAVG
FEQTIPFVHGSQGCVAYYRSHFSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANTYSMYKPKMISVSTTCMAEVIGDD
LNAFIKTAKEKGSVPQEYDVPFAHTPAFVGSHITGYDNVLKGIFEHFWDGKAGTAPKLERVPNEKINFIGGFDGYTVGNL
REIKRLFGLMGVEYTILADNSEVWDTPTDGEFRMYDGGTTLEEAADALHAKATISMQEFCTEKTLPLIANHGQEVVAFNH
PVGVKGTDEFLMAISRISGKEIPEALAKERGRLVDAIADSNAHIHGKKFAIYGDPDLCMGLAAFLLELGAEPTHVLATNG
NKKWADKMQELFDSSPFGQNCQVYPGRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPVFDRHHHHRRPVW
GYQGGMNVLITILDKIFDTIDQNTIVPAKTDYSFDIIR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 57970; Mature: 57839

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
PQNADNVLDHFELFRGPEYQQMLANKKKLFENPRDPAEVERVREWAKTPEYKEKNFARE
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
ALTVNPAKACQPLGAVFAAVGFEQTIPFVHGSQGCVAYYRSHFSRHFKEPSSCVSSSMTE
HHCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
DAAVFGGLNNMIDGLANTYSMYKPKMISVSTTCMAEVIGDDLNAFIKTAKEKGSVPQEYD
HHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VPFAHTPAFVGSHITGYDNVLKGIFEHFWDGKAGTAPKLERVPNEKINFIGGFDGYTVGN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCC
LREIKRLFGLMGVEYTILADNSEVWDTPTDGEFRMYDGGTTLEEAADALHAKATISMQEF
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHH
CTEKTLPLIANHGQEVVAFNHPVGVKGTDEFLMAISRISGKEIPEALAKERGRLVDAIAD
HHHCCCHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCEEEHHHC
SNAHIHGKKFAIYGDPDLCMGLAAFLLELGAEPTHVLATNGNKKWADKMQELFDSSPFGQ
CCCEEECCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
NCQVYPGRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPVFDRHHHHRRPV
CCEECCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCC
WGYQGGMNVLITILDKIFDTIDQNTIVPAKTDYSFDIIR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157954; 12597275 [H]