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The map label for this gene is nifD [H]

Identifier: 154244047

GI number: 154244047

Start: 97375

End: 98880

Strand: Direct

Name: nifD [H]

Synonym: Xaut_0089

Alternate gene names: 154244047

Gene position: 97375-98880 (Clockwise)

Preceding gene: 154244046

Following gene: 154244048

Centisome position: 1.83

GC content: 61.82

Gene sequence:

>1506_bases
ATGAGCTTGGCCCAACCGCAAAGCGTTGCTGAAATCAAGGCGCGTAACAAAGAACTCATCGCGGAAGTTCTCAAGGTTTA
TCCCGAGAAGACCGCGAAGCGCCGCGCGAAGCACCTCAACGTCCACGAGTCCGGCAAGTCCGATTGCGGCGTGAAGTCGA
ACATCAAGTCCATCCCGGGCGTGATGACGATCCGCGGCTGCGCGTATGCCGGTTCCAAGGGCGTGGTGTGGGGTCCCATC
AAGGACATGATCCACATCTCCCACGGCCCGGTGGGCTGCGGCCAGTACAGCTGGGCCGCCCGCCGCAACTACTACATCGG
CACGACGGGCATCGACACCTTCGTGACCATGCAGTTCACCTCGGACTTCCAGGAAAAGGACATCGTCTTCGGCGGCGACA
AGAAGCTCGCCAAGATCATGGACGAAATCCAGGACCTGTTCCCGCTGAACAACGGCATCACCGTCCAGTCCGAGTGCCCG
ATCGGCCTCATCGGCGACGACATCGAGGCCGTGTCCAAGGCGAAGTCCAAGGAATACGACAACAAGACCATCGTGCCGGT
CCGCTGCGAGGGCTTCCGCGGCGTGTCCCAGTCGCTCGGCCATCACATCGCCAACGATGCGATCCGGGACTGGGTGTTCG
ACAAGATGGACCCGAATGCGGCCCCCCGCTTCGAGCCGTCCCCGTATGACGTCGCCATCATCGGCGACTACAACATCGGT
GGCGACGCCTGGTCTTCGCGCATCCTGCTCGAGGAAATGGGCCTGCGCGTGATCGCACAGTGGTCCGGCGACGGCTCGCT
TGCCGAGCTCGAGGCGACTCCGAAGGCGAAGCTGAACGTCCTGCACTGCTACCGGTCCATGAACTACATCTCCCGCCACA
TGGAAGAGAAGTTCGGTATCCCGTGGTGCGAGTACAACTTCTTCGGCCCGTCCAAGATCGCCGAGTCGCTGCGTAAGATC
GCCGCTTACTTCGACGACAAGATCAAGGAAGGCGCCGAGCGCGTCATCGCCAAGTACCAGCCCCTCATGGACGCGGTCAT
TGCCAAGTACCGTCCGCGGCTCGAGGGCAAGACCGTCATGCTGTACGTGGGCGGCCTGCGTCCCCGTCACGTGATCGGCG
CCTACGAAGACCTCGGCATGGAAGTGGTCGGCACCGGCTACGAGTTCGGCCACAACGACGACTATCAGCGCACCGCCCAG
CACTACGTCAAGGATGGCACGATCATCTATGACGACGTGACCGGCTATGAGTTCGAGAAGTTCGTCGAGAAGGTCCAGCC
CGATCTCGTCGGCTCGGGCATCAAGGAAAAGTACGTGTTCCAGAAGATGGGTGTGCCGTTCCGGCAGATGCACTCCTGGG
ACTATTCCGGCCCGTATCACGGCTATGACGGCTTCGCCATCTTCGCCCGCGACATGGACATGGCCATCAACTCCCCCGTC
TGGAAGATGACCAAGGCTCCGTGGAAGCAGGCTCCCGCGCAGCCGGGCCTGCTCGCGGCCGAGTGA

Upstream 100 bases:

>100_bases
ACCGCCGCCGAGCTCACCGCCTGAGGCTGACGCAGCGAGGCCGGCCCCAATTGCGGGGTCGGCCTTCATCTGGCACCCAA
AACGAGAGCGAGATTGCGAA

Downstream 100 bases:

>100_bases
CCGCCTGAGCCCGAAACGCCCCTCCCCTTTTTGGGGAGGGGCACCTGGCGGGCTTGATCCGTTTTGACCCACCCATAGGG
GTTCGAGATGCCACAGAATG

Product: nitrogenase molybdenum-iron protein subunit alpha

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSLAQPQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPGVMTIRGCAYAGSKGVVWGPI
KDMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIQDLFPLNNGITVQSECP
IGLIGDDIEAVSKAKSKEYDNKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKMDPNAAPRFEPSPYDVAIIGDYNIG
GDAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGIPWCEYNFFGPSKIAESLRKI
AAYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVMLYVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTAQ
HYVKDGTIIYDDVTGYEFEKFVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPV
WKMTKAPWKQAPAQPGLLAAE

Sequences:

>Translated_501_residues
MSLAQPQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPGVMTIRGCAYAGSKGVVWGPI
KDMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIQDLFPLNNGITVQSECP
IGLIGDDIEAVSKAKSKEYDNKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKMDPNAAPRFEPSPYDVAIIGDYNIG
GDAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGIPWCEYNFFGPSKIAESLRKI
AAYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVMLYVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTAQ
HYVKDGTIIYDDVTGYEFEKFVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPV
WKMTKAPWKQAPAQPGLLAAE
>Mature_500_residues
SLAQPQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPGVMTIRGCAYAGSKGVVWGPIK
DMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIQDLFPLNNGITVQSECPI
GLIGDDIEAVSKAKSKEYDNKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKMDPNAAPRFEPSPYDVAIIGDYNIGG
DAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGIPWCEYNFFGPSKIAESLRKIA
AYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVMLYVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTAQH
YVKDGTIIYDDVTGYEFEKFVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVW
KMTKAPWKQAPAQPGLLAAE

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 56137; Mature: 56006

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAQPQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPG
CCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCC
VMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFT
EEEEEEEEECCCCCCEEECHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEEEEEEC
SDFQEKDIVFGGDKKLAKIMDEIQDLFPLNNGITVQSECPIGLIGDDIEAVSKAKSKEYD
CCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHHHHHCCC
NKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKMDPNAAPRFEPSPYDVAIIGDYNIG
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC
GDAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGI
CHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PWCEYNFFGPSKIAESLRKIAAYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVM
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LYVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTAQHYVKDGTIIYDDVTGYEFEK
EEECCCCCHHHCCCHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHH
FVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPV
HHHHHCHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEEEECHHHHHCCCH
WKMTKAPWKQAPAQPGLLAAE
HHHHCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
SLAQPQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPG
CCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCC
VMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFT
EEEEEEEEECCCCCCEEECHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEEEEEEC
SDFQEKDIVFGGDKKLAKIMDEIQDLFPLNNGITVQSECPIGLIGDDIEAVSKAKSKEYD
CCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHHHHHCCC
NKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKMDPNAAPRFEPSPYDVAIIGDYNIG
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCC
GDAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGI
CHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PWCEYNFFGPSKIAESLRKIAAYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVM
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LYVGGLRPRHVIGAYEDLGMEVVGTGYEFGHNDDYQRTAQHYVKDGTIIYDDVTGYEFEK
EEECCCCCHHHCCCHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHH
FVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPV
HHHHHCHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEEEECHHHHHCCCH
WKMTKAPWKQAPAQPGLLAAE
HHHHCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157954; 12597275; 6588133 [H]