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The map label for this gene is prfA [H]

Identifier: 153952433

GI number: 153952433

Start: 1735422

End: 1736489

Strand: Direct

Name: prfA [H]

Synonym: JJD26997_1966

Alternate gene names: 153952433

Gene position: 1735422-1736489 (Clockwise)

Preceding gene: 153951103

Following gene: 153952500

Centisome position: 94.06

GC content: 34.93

Gene sequence:

>1068_bases
ATGTTAGCTAGCAAACTAGATCCTTTTTTAAAACGCTTTGAAGAGTTAAATTCTCTTCTTAGCAGTTCTGATATACTCAA
TGATATCAGTAAAATGACTACACTCTCTAAAGAACAAAAAAATTTAGAACCTATCGTTTTAAAAACTAAAGAGTATTTAA
AAACTTTAGACAATATAGAAGAAAATAAAGCTTTGCTAAACGATCCAGAACTTGGAGAACTTGCAAAAGAAGAACTAAAA
ACTTTAGAAGAACTAAAACCAAAACTTGAAGAAGAAATTAAAATTTTACTTCTTCCTAAAGATCTCAATGATGAAAGAAA
TATTTTTCTTGAAATTCGTGCAGGCACAGGTGGCGATGAAGCATCTTTATTTGTAGGAGATCTTGTAAAAGCTTATGCAA
GATATGCTGAAAATCGTGGCTATAAACTTGAGATTGTAAGCTCTAGTGAAGGAAGTGTAGGTGGATTCAAAGAAATCATC
ATGCTTGTTAAAGGAACAGGGGCTTATTCAAGACTTAAATATGAAGGTGGAACGCACCGTGTTCAAAGAGTTCCTCAAAC
AGAATCCCAAGGACGCGTGCATACTTCTGCTATCACCGTGGCAGTAATGCCAGAAGTTGATGATATAGAAATCGAAATCA
ATCCAAACGATCTTAAAGTTGATGTGATGCGTTCTTCTGGACATGGTGGACAAAGTGTAAACACTACTGATTCAGCTGTT
CGTATCACTCATATCCCTACAGGTATAGTTGTAGTCAATCAAGATGGAAAAAGCCAACACAAAAATAAAGAAAGTGCGAT
GAAAGTCCTAAAAGCAAGGCTTTATGAAATGCAAGAGAACGAAAGACTAGCAAAAGAAAGTGAAGCAAGAAAATCTCAAG
TAGGAAGCGGAGATAGAAGCGAACGCATTCGCACTTACAATTTTCCACAAAATCGTATCAGCGACCATAGAATAAATCTT
ACTTTATATCGCTTAGATGCCATTATGCAAGATGGGCTTTTTGATGAAATCATAGAACCTTTAATCACTCATCATCAAGC
CCAAGCTTTACAAGAACAAAATTTATAA

Upstream 100 bases:

>100_bases
GGTAAGTCACGGTTTTATTAAAAAACAAACTGCTTCTCGCCGTGTAAGCCGCTTAGCCCTTTTAGTAAATAAAATCGCAT
AATTTTCTATATAGTTTTTA

Downstream 100 bases:

>100_bases
GATTTTATATAAATTAATGCTTATGTCGATTTCCACTAGCACCTACATGTGCGACATTTCCATTTTCTATATCATAAGCT
TGACCAAAACCTTTTACAAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLASKLDPFLKRFEELNSLLSSSDILNDISKMTTLSKEQKNLEPIVLKTKEYLKTLDNIEENKALLNDPELGELAKEELK
TLEELKPKLEEEIKILLLPKDLNDERNIFLEIRAGTGGDEASLFVGDLVKAYARYAENRGYKLEIVSSSEGSVGGFKEII
MLVKGTGAYSRLKYEGGTHRVQRVPQTESQGRVHTSAITVAVMPEVDDIEIEINPNDLKVDVMRSSGHGGQSVNTTDSAV
RITHIPTGIVVVNQDGKSQHKNKESAMKVLKARLYEMQENERLAKESEARKSQVGSGDRSERIRTYNFPQNRISDHRINL
TLYRLDAIMQDGLFDEIIEPLITHHQAQALQEQNL

Sequences:

>Translated_355_residues
MLASKLDPFLKRFEELNSLLSSSDILNDISKMTTLSKEQKNLEPIVLKTKEYLKTLDNIEENKALLNDPELGELAKEELK
TLEELKPKLEEEIKILLLPKDLNDERNIFLEIRAGTGGDEASLFVGDLVKAYARYAENRGYKLEIVSSSEGSVGGFKEII
MLVKGTGAYSRLKYEGGTHRVQRVPQTESQGRVHTSAITVAVMPEVDDIEIEINPNDLKVDVMRSSGHGGQSVNTTDSAV
RITHIPTGIVVVNQDGKSQHKNKESAMKVLKARLYEMQENERLAKESEARKSQVGSGDRSERIRTYNFPQNRISDHRINL
TLYRLDAIMQDGLFDEIIEPLITHHQAQALQEQNL
>Mature_355_residues
MLASKLDPFLKRFEELNSLLSSSDILNDISKMTTLSKEQKNLEPIVLKTKEYLKTLDNIEENKALLNDPELGELAKEELK
TLEELKPKLEEEIKILLLPKDLNDERNIFLEIRAGTGGDEASLFVGDLVKAYARYAENRGYKLEIVSSSEGSVGGFKEII
MLVKGTGAYSRLKYEGGTHRVQRVPQTESQGRVHTSAITVAVMPEVDDIEIEINPNDLKVDVMRSSGHGGQSVNTTDSAV
RITHIPTGIVVVNQDGKSQHKNKESAMKVLKARLYEMQENERLAKESEARKSQVGSGDRSERIRTYNFPQNRISDHRINL
TLYRLDAIMQDGLFDEIIEPLITHHQAQALQEQNL

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=297, Percent_Identity=46.4646464646465, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI34577120, Length=331, Percent_Identity=37.1601208459215, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI166795305, Length=191, Percent_Identity=43.979057591623, Blast_Score=172, Evalue=3e-43,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=53.6516853932584, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=41.0714285714286, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=299, Percent_Identity=36.1204013377926, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=348, Percent_Identity=42.2413793103448, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI19921226, Length=288, Percent_Identity=41.6666666666667, Blast_Score=214, Evalue=6e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39983; Mature: 39983

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASKLDPFLKRFEELNSLLSSSDILNDISKMTTLSKEQKNLEPIVLKTKEYLKTLDNIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCHH
ENKALLNDPELGELAKEELKTLEELKPKLEEEIKILLLPKDLNDERNIFLEIRAGTGGDE
HCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCCCCC
ASLFVGDLVKAYARYAENRGYKLEIVSSSEGSVGGFKEIIMLVKGTGAYSRLKYEGGTHR
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHEEEECCCCHH
VQRVPQTESQGRVHTSAITVAVMPEVDDIEIEINPNDLKVDVMRSSGHGGQSVNTTDSAV
HHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEE
RITHIPTGIVVVNQDGKSQHKNKESAMKVLKARLYEMQENERLAKESEARKSQVGSGDRS
EEEECCCEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ERIRTYNFPQNRISDHRINLTLYRLDAIMQDGLFDEIIEPLITHHQAQALQEQNL
CCCEEECCCHHHCCCCEEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLASKLDPFLKRFEELNSLLSSSDILNDISKMTTLSKEQKNLEPIVLKTKEYLKTLDNIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCHH
ENKALLNDPELGELAKEELKTLEELKPKLEEEIKILLLPKDLNDERNIFLEIRAGTGGDE
HCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCCCCC
ASLFVGDLVKAYARYAENRGYKLEIVSSSEGSVGGFKEIIMLVKGTGAYSRLKYEGGTHR
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHEEEECCCCHH
VQRVPQTESQGRVHTSAITVAVMPEVDDIEIEINPNDLKVDVMRSSGHGGQSVNTTDSAV
HHCCCCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEE
RITHIPTGIVVVNQDGKSQHKNKESAMKVLKARLYEMQENERLAKESEARKSQVGSGDRS
EEEECCCEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ERIRTYNFPQNRISDHRINLTLYRLDAIMQDGLFDEIIEPLITHHQAQALQEQNL
CCCEEECCCHHHCCCCEEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA