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The map label for this gene is pycB [H]

Identifier: 153952195

GI number: 153952195

Start: 780962

End: 782761

Strand: Direct

Name: pycB [H]

Synonym: JJD26997_0881

Alternate gene names: 153952195

Gene position: 780962-782761 (Clockwise)

Preceding gene: 153951231

Following gene: 153951612

Centisome position: 42.33

GC content: 32.61

Gene sequence:

>1800_bases
ATGGCTAAAAAATTCATCGATGTAATGGATACAAGCTTTAGGGATGGTTTTCAGTCTGTTTACGGGGCTAGGGTTTTAAT
GGATGATTTTTTTCCTGCAGTAGAAGCAGCAAAAGAAGCAGGCATTACACATTTTGAATTTGGCGGTGGTGCTAGATTTC
AAAGTTTGTATTTTTATCTTAATGAAGATGCTTTTACCATGATGGATAGATTTAGAGCTATTGTAGGAAAAGATGCCAAT
CTTCAAACTTTAGCAAGAGGGGTAAATACCGTTACTTTAGATACTGGAAGTAGTGAGCTTATTGATTTACACGCAAAACT
TTTTGCAAAACACGGTACTACAACGATAAGAAATTTTGATGCCCTTAATGATGTAAATAATTTAAAATTTAGTGGTGAAT
GTATAGTAAAATATGGGTTAAAACATGAAATTACTATTACTTTAATGGATTTACCGCCAAATTGCAAAGGTGCTCATGAT
GTTGCTTTTTATGAAAAAATTTTAAAAGAAATTTTAACAGCAGAAATTCCTTTTCATAGTATTTGTTTCAAAGATGCAAG
TGGAACTTCTAATCCAAATAAAATTTATGAAACCATTAAAATGGCTAGAAAAATTTTACCTCAAGATACACATATTAGAC
TTCATACGCATGAAACTGCAGGAGTGAGTATAGCTTGCTATCTTGCAGCGCTTGAAGCAGGGGTTAATGGCATCGATTTG
GCTGCAGCTCCAGTAAGTGGTGGAACTTCTCAGCCTGATATTTTAACCATGATGCATGCTTTAAAAGGTAAAGATTATGA
TTTAGGCGGACTTGAAGAAGAAAAAATTTTAAGATACGAAGAAGTTTTAAAAGATTGTTTAAAAGAGTATTTTTTACCAC
CTGAAGCTACTATGGTAAATCCACTTATTCCTTTTTCTCCTATGCCAGGAGGAGCTTTAACAGCAAATACACAAATGATG
AGAGATAATAATATTTTAGATAAATTTCCACAGGTCATTCATGCTATGAGAGAAGTTGTAGAAAAAGGTGGATTTGGTAC
TTCTGTTACTCCAGTTTCTCAATTTTATTTTCAACAAGCTTTTAATAATGTGATGTTTGGACCTTGGAAAAAAATCGCAG
AAGGTTATGGAAAAATGGTACTTGGATATTTTGGAAAAACTCCAGTTGCGCCTGATGCAAACATTATTGAACTTGCCTCA
AAGCAGCTTAATTTAGAGCCTACTACAGAGCTTGCTATCAATATAGCTGATAAAGATGAGAGTAAAAGCATGGCTTATAC
TAAAGCTTCACTTGAAAAAGAAGGTATAGAAACAAATGAAGAGAATATATTTATTGCAGCAGCTTGTAGAGAAAAAGGTA
TAGCATTTTTAAAAGGAGAGGCAAAGGTAAATGTACGCAAATTAGCTAGTATGCCAAAACCTTCAAGCATAGATGAAAAC
AAATTTACTGTAGCTGTAAATGGTAACAAATATCATGTAGAGGTAAACTATGGTTTTGATAAAGATGTTAATGTTAAAAG
TGTAAAAAAAGTAGAAAAAAATAAAAATATTATTTCTTCAAATTCAACAAGTTCTGTAGATGCTGAAAATGAAGTTTTCG
CAGGTATTTCAGGTAATGTTTTTAAAATTTATGTTAACGAAGGTGAAGAAGTAAAATCAGGTCAAGCCATCATGGTTTTA
GAAGCAATGAAAATGGAGATTGAGGTTAATGCCCCAAAAGATGGAATTATTTCAGAGCTTTGTATTAAAATAGGCGATAC
TGTTAACGAAGGCGAAGTATTAGCTATTTACAAGAATTAA

Upstream 100 bases:

>100_bases
TGTTTTTTATTCGTTTTATATCGCCTATAGCAATAATTTTGGTAATGTGTTATCAAATTTTTGTATAATCAATGAAAAAA
TATTTTTTTAAGGAAAAATA

Downstream 100 bases:

>100_bases
AGTGAGGGAAAAATGAAAAAATTTGATAATTTAGGTTTGGATAATATAAAGGAAATTTTTCATAATTTAAGTTATGATGA
ATTAAATGCTCATGAAAAAG

Product: oxaloacetate decarboxylase, alpha subunit, putative

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MAKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYLNEDAFTMMDRFRAIVGKDAN
LQTLARGVNTVTLDTGSSELIDLHAKLFAKHGTTTIRNFDALNDVNNLKFSGECIVKYGLKHEITITLMDLPPNCKGAHD
VAFYEKILKEILTAEIPFHSICFKDASGTSNPNKIYETIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVNGIDL
AAAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVNPLIPFSPMPGGALTANTQMM
RDNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQAFNNVMFGPWKKIAEGYGKMVLGYFGKTPVAPDANIIELAS
KQLNLEPTTELAINIADKDESKSMAYTKASLEKEGIETNEENIFIAAACREKGIAFLKGEAKVNVRKLASMPKPSSIDEN
KFTVAVNGNKYHVEVNYGFDKDVNVKSVKKVEKNKNIISSNSTSSVDAENEVFAGISGNVFKIYVNEGEEVKSGQAIMVL
EAMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN

Sequences:

>Translated_599_residues
MAKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYLNEDAFTMMDRFRAIVGKDAN
LQTLARGVNTVTLDTGSSELIDLHAKLFAKHGTTTIRNFDALNDVNNLKFSGECIVKYGLKHEITITLMDLPPNCKGAHD
VAFYEKILKEILTAEIPFHSICFKDASGTSNPNKIYETIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVNGIDL
AAAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVNPLIPFSPMPGGALTANTQMM
RDNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQAFNNVMFGPWKKIAEGYGKMVLGYFGKTPVAPDANIIELAS
KQLNLEPTTELAINIADKDESKSMAYTKASLEKEGIETNEENIFIAAACREKGIAFLKGEAKVNVRKLASMPKPSSIDEN
KFTVAVNGNKYHVEVNYGFDKDVNVKSVKKVEKNKNIISSNSTSSVDAENEVFAGISGNVFKIYVNEGEEVKSGQAIMVL
EAMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN
>Mature_598_residues
AKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYLNEDAFTMMDRFRAIVGKDANL
QTLARGVNTVTLDTGSSELIDLHAKLFAKHGTTTIRNFDALNDVNNLKFSGECIVKYGLKHEITITLMDLPPNCKGAHDV
AFYEKILKEILTAEIPFHSICFKDASGTSNPNKIYETIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVNGIDLA
AAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVNPLIPFSPMPGGALTANTQMMR
DNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQAFNNVMFGPWKKIAEGYGKMVLGYFGKTPVAPDANIIELASK
QLNLEPTTELAINIADKDESKSMAYTKASLEKEGIETNEENIFIAAACREKGIAFLKGEAKVNVRKLASMPKPSSIDENK
FTVAVNGNKYHVEVNYGFDKDVNVKSVKKVEKNKNIISSNSTSSVDAENEVFAGISGNVFKIYVNEGEEVKSGQAIMVLE
AMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=628, Percent_Identity=24.5222929936306, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI106049295, Length=628, Percent_Identity=24.5222929936306, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI106049292, Length=628, Percent_Identity=24.5222929936306, Blast_Score=111, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17562816, Length=286, Percent_Identity=27.972027972028, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319695, Length=636, Percent_Identity=25.4716981132075, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321376, Length=634, Percent_Identity=24.1324921135647, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24652212, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652210, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652214, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921944, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652216, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281363050, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652224, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652222, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652220, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652218, Length=640, Percent_Identity=26.40625, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 65964; Mature: 65833

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEE
NEDAFTMMDRFRAIVGKDANLQTLARGVNTVTLDTGSSELIDLHAKLFAKHGTTTIRNFD
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCH
ALNDVNNLKFSGECIVKYGLKHEITITLMDLPPNCKGAHDVAFYEKILKEILTAEIPFHS
HHCCCCCCEECCCEEEEECCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHH
ICFKDASGTSNPNKIYETIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVNGIDL
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEE
AAAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVN
EECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEC
PLIPFSPMPGGALTANTQMMRDNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQA
CCCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FNNVMFGPWKKIAEGYGKMVLGYFGKTPVAPDANIIELASKQLNLEPTTELAINIADKDE
HCCCCCCCHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
SKSMAYTKASLEKEGIETNEENIFIAAACREKGIAFLKGEAKVNVRKLASMPKPSSIDEN
CCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHCCCCCCCCCCC
KFTVAVNGNKYHVEVNYGFDKDVNVKSVKKVEKNKNIISSNSTSSVDAENEVFAGISGNV
EEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCEEECCCCCE
FKIYVNEGEEVKSGQAIMVLEAMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN
EEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
>Mature Secondary Structure 
AKKFIDVMDTSFRDGFQSVYGARVLMDDFFPAVEAAKEAGITHFEFGGGARFQSLYFYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEE
NEDAFTMMDRFRAIVGKDANLQTLARGVNTVTLDTGSSELIDLHAKLFAKHGTTTIRNFD
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCH
ALNDVNNLKFSGECIVKYGLKHEITITLMDLPPNCKGAHDVAFYEKILKEILTAEIPFHS
HHCCCCCCEECCCEEEEECCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHH
ICFKDASGTSNPNKIYETIKMARKILPQDTHIRLHTHETAGVSIACYLAALEAGVNGIDL
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEE
AAAPVSGGTSQPDILTMMHALKGKDYDLGGLEEEKILRYEEVLKDCLKEYFLPPEATMVN
EECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEC
PLIPFSPMPGGALTANTQMMRDNNILDKFPQVIHAMREVVEKGGFGTSVTPVSQFYFQQA
CCCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FNNVMFGPWKKIAEGYGKMVLGYFGKTPVAPDANIIELASKQLNLEPTTELAINIADKDE
HCCCCCCCHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCC
SKSMAYTKASLEKEGIETNEENIFIAAACREKGIAFLKGEAKVNVRKLASMPKPSSIDEN
CCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHCCCCCCCCCCC
KFTVAVNGNKYHVEVNYGFDKDVNVKSVKKVEKNKNIISSNSTSSVDAENEVFAGISGNV
EEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCEEECCCCCE
FKIYVNEGEEVKSGQAIMVLEAMKMEIEVNAPKDGIISELCIKIGDTVNEGEVLAIYKN
EEEEECCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]