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The map label for this gene is pckA [H]

Identifier: 153951612

GI number: 153951612

Start: 782774

End: 784348

Strand: Direct

Name: pckA [H]

Synonym: JJD26997_0882

Alternate gene names: 153951612

Gene position: 782774-784348 (Clockwise)

Preceding gene: 153952195

Following gene: 153951734

Centisome position: 42.42

GC content: 33.02

Gene sequence:

>1575_bases
ATGAAAAAATTTGATAATTTAGGTTTGGATAATATAAAGGAAATTTTTCATAATTTAAGTTATGATGAATTAAATGCTCA
TGAAAAAGTAAACAATGAAGGTTTAAGTACGGATAATGATACTTTTTGTGTAGATACAGGCATTTTTACAGGTAGAAGTC
CTAAAGATAAATATTTTGTAAAACAAGATCCATCTAGCAAATATATAGCTTGGGGAAAGGTAAATCAACCTATTACAAAA
GAATTATTTGATAAGCTTTTGACTAAAGCAAAACAAGAATTAAGTGGAAAGAAAATTTATGTTCAAGATGCTTTTTGCGG
TGCTTCTTTACAAAGTCGTAAAGCGGTAAGATTTGTTACTGAAATTGCATGGCAAGCGCATTTTGTTAAGAATATGTTTA
TTCGCCCAAGTCAAGAGGAACTTGAGAATTTCAAAGCAGATTTTATCGTTTATAATGCTTGCAAATGTATCAATGAAGAT
TATAAACAAGATGGTTTAAATTCAGAAGTTTTTGTAATCTTTAATGTTGAAGAAAATATAGCAGTTATTGGTGGGACTTG
GTATGGTGGAGAAATGAAAAAAGGTATTTTTTCTATGATGAATTATTGGTTGCCGCTAGAAAATAAACTTTCTATGCATT
GCAGTGCAAATGTCGGAGAAAAGGGTGATGTAGCACTTTTCTTTGGACTTAGTGGCACTGGAAAAACTACGCTTTCAACA
GATCCAAAAAGAAAACTTATAGGTGATGATGAGCATGGCTGGGATGATGAAGGTGTATTTAATTTTGAGGGCGGTTGTTA
CGCAAAAACAATTAATCTTGATCCTGAGCACGAACCTGAAATTTATGGTGCGATTAAAAGAAATGCTCTCTTAGAAAATG
TAGTTTTAAGAGCCGATAAGAGTGTTGATTATGCTGATGCTTCAAAAACAGAAAATACAAGAGTTTCTTATCCTATAGAG
CATATTGAAAATCATGAGCCTAGCTTAAAAGCAGGTCATCCAAAAAATATTATATTTTTAAGTGCAGATGCTTTTGGAAT
TTTACCTCCGGTGAGCAAACTAAGCAAAGAGCAGGCTATGTATTATTTTTTAAGCGGTTATACAGCAAAAGTAGCTGGAA
CTGAGCGTGGTATAACAGAGCCTCAAGCAACTTTTTCAGCTTGCTTTGGGGAGCCTTTTATGCCTTTACATCCAACGGTT
TATGCGAGATTACTTGGTGAAAAGATTGAAAAATATGAAGTTAATGTTTATCTTGTTAATACAGGTTGGAGTGGTGGAAG
CTACGGTGTGGGTAAAAGAATGAGCATTAAGGCTACTAGGGCTTGCATTAATGCTATTTTAGATGGAAGCATTACAAAAT
GTGAATTTGAAAATTTTGAAGTGTTTGATTTGGCTATTCCAAAAGCTTTAGAAGGTGTAGAAAGCACGCTTTTAAATCCT
ATAAATACTTGGTCGGATAAAAATGCCTATACCGAAACAAGAGATAAATTAGCCCATATGTTTGTGCAAAATTTTAAACG
CTATGAAGATGTCAAAGAAGGCATTGAATTTAGCAAATTTGGACCAAAAATTTAA

Upstream 100 bases:

>100_bases
CCCAAAAGATGGAATTATTTCAGAGCTTTGTATTAAAATAGGCGATACTGTTAACGAAGGCGAAGTATTAGCTATTTACA
AGAATTAAAGTGAGGGAAAA

Downstream 100 bases:

>100_bases
GGCGTTTAAATGAAAAATGAAATGTGGTCAGGACGCTTTAGCGGTGCAAGTGATGAGCTTCTAAAGGAATTTAATGCGAG
TTTGAATGTAGATAAAACTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MKKFDNLGLDNIKEIFHNLSYDELNAHEKVNNEGLSTDNDTFCVDTGIFTGRSPKDKYFVKQDPSSKYIAWGKVNQPITK
ELFDKLLTKAKQELSGKKIYVQDAFCGASLQSRKAVRFVTEIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINED
YKQDGLNSEVFVIFNVEENIAVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKGDVALFFGLSGTGKTTLST
DPKRKLIGDDEHGWDDEGVFNFEGGCYAKTINLDPEHEPEIYGAIKRNALLENVVLRADKSVDYADASKTENTRVSYPIE
HIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTV
YARLLGEKIEKYEVNVYLVNTGWSGGSYGVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESTLLNP
INTWSDKNAYTETRDKLAHMFVQNFKRYEDVKEGIEFSKFGPKI

Sequences:

>Translated_524_residues
MKKFDNLGLDNIKEIFHNLSYDELNAHEKVNNEGLSTDNDTFCVDTGIFTGRSPKDKYFVKQDPSSKYIAWGKVNQPITK
ELFDKLLTKAKQELSGKKIYVQDAFCGASLQSRKAVRFVTEIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINED
YKQDGLNSEVFVIFNVEENIAVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKGDVALFFGLSGTGKTTLST
DPKRKLIGDDEHGWDDEGVFNFEGGCYAKTINLDPEHEPEIYGAIKRNALLENVVLRADKSVDYADASKTENTRVSYPIE
HIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTV
YARLLGEKIEKYEVNVYLVNTGWSGGSYGVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESTLLNP
INTWSDKNAYTETRDKLAHMFVQNFKRYEDVKEGIEFSKFGPKI
>Mature_524_residues
MKKFDNLGLDNIKEIFHNLSYDELNAHEKVNNEGLSTDNDTFCVDTGIFTGRSPKDKYFVKQDPSSKYIAWGKVNQPITK
ELFDKLLTKAKQELSGKKIYVQDAFCGASLQSRKAVRFVTEIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINED
YKQDGLNSEVFVIFNVEENIAVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKGDVALFFGLSGTGKTTLST
DPKRKLIGDDEHGWDDEGVFNFEGGCYAKTINLDPEHEPEIYGAIKRNALLENVVLRADKSVDYADASKTENTRVSYPIE
HIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAMYYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTV
YARLLGEKIEKYEVNVYLVNTGWSGGSYGVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESTLLNP
INTWSDKNAYTETRDKLAHMFVQNFKRYEDVKEGIEFSKFGPKI

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=535, Percent_Identity=56.8224299065421, Blast_Score=624, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6322950, Length=487, Percent_Identity=48.870636550308, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58988; Mature: 58988

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFDNLGLDNIKEIFHNLSYDELNAHEKVNNEGLSTDNDTFCVDTGIFTGRSPKDKYFV
CCCCCCCCHHHHHHHHHCCCHHHCCHHHHCCCCCCCCCCCCEEEECCEECCCCCCCCEEE
KQDPSSKYIAWGKVNQPITKELFDKLLTKAKQELSGKKIYVQDAFCGASLQSRKAVRFVT
EECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINEDYKQDGLNSEVFVIFNVEENI
HHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEHHHHHHCHHHHHCCCCCCEEEEEEECCCE
AVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKGDVALFFGLSGTGKTTLST
EEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
DPKRKLIGDDEHGWDDEGVFNFEGGCYAKTINLDPEHEPEIYGAIKRNALLENVVLRADK
CCCHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SVDYADASKTENTRVSYPIEHIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAM
CCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHE
YYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTVYARLLGEKIEKYEVNVYLVN
EEEECCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEE
TGWSGGSYGVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESTLLNP
CCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCC
INTWSDKNAYTETRDKLAHMFVQNFKRYEDVKEGIEFSKFGPKI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKFDNLGLDNIKEIFHNLSYDELNAHEKVNNEGLSTDNDTFCVDTGIFTGRSPKDKYFV
CCCCCCCCHHHHHHHHHCCCHHHCCHHHHCCCCCCCCCCCCEEEECCEECCCCCCCCEEE
KQDPSSKYIAWGKVNQPITKELFDKLLTKAKQELSGKKIYVQDAFCGASLQSRKAVRFVT
EECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EIAWQAHFVKNMFIRPSQEELENFKADFIVYNACKCINEDYKQDGLNSEVFVIFNVEENI
HHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEHHHHHHCHHHHHCCCCCCEEEEEEECCCE
AVIGGTWYGGEMKKGIFSMMNYWLPLENKLSMHCSANVGEKGDVALFFGLSGTGKTTLST
EEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
DPKRKLIGDDEHGWDDEGVFNFEGGCYAKTINLDPEHEPEIYGAIKRNALLENVVLRADK
CCCHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SVDYADASKTENTRVSYPIEHIENHEPSLKAGHPKNIIFLSADAFGILPPVSKLSKEQAM
CCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHE
YYFLSGYTAKVAGTERGITEPQATFSACFGEPFMPLHPTVYARLLGEKIEKYEVNVYLVN
EEEECCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEE
TGWSGGSYGVGKRMSIKATRACINAILDGSITKCEFENFEVFDLAIPKALEGVESTLLNP
CCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCEEEEEECCHHHHHHHHHHHCCC
INTWSDKNAYTETRDKLAHMFVQNFKRYEDVKEGIEFSKFGPKI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA