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The map label for this gene is pheT [H]

Identifier: 153952099

GI number: 153952099

Start: 812060

End: 814381

Strand: Direct

Name: pheT [H]

Synonym: JJD26997_0917

Alternate gene names: 153952099

Gene position: 812060-814381 (Clockwise)

Preceding gene: 153952279

Following gene: 153951777

Centisome position: 44.01

GC content: 28.12

Gene sequence:

>2322_bases
ATGATAATTACTAAAAGTTGGTTGAATGATTGGTTAGAACTTGAAGAAATAAGTTCAGATAAAATAGCTAAAACTTTAAA
CTCTATAGGTATAGAAGTTGATAGGGTTGGCGCTTTAAAGTCTCCAGATAAAGTTGTTGTAGGCTATGTTAAAGAAAAAA
TTAAACATGAAAATTCAGATAAGTTAAGCATTTGTCAAGTTGATATCGGAAGTGAAACCTTGCAAATTGTCTGCGGTGCT
GCAAATGTTGATGCAGGACAATTTGTAGCTGTTGCAACTAAAGGTGCTATCATGCCAAACGGTATGGAAATAAAAGAAGC
AAAATTACGAGGAGTGGATTCTTGTGGTATGCTTTGTTCTAGTTTGGAACTGGGTTTTGAAAAAATCAATGAAGGAATTA
TGCTTTTAGATGAGAGCATAGGGAAATTAGAACTTGGGAGAGCTTTAAATACTTATGAAATTTTTAATGATGAGCTAATA
GAAGTAGAACTTACTCCTAATAGGGGTGATTGTTTAAGTATTTATGGTGTTGCTCGTGATTTAGCTACAGCTTTAAATTT
AAATTTAAAAGAAGCAAAACCATTCAAAGAAAGTGAAAATGTTTTAGGTATTGGTAGAATTTTGCGCTTAGCAGCTGAGA
AAGAACTTAATGGACTTTATAATTATCGAGCTATAGAGCTAAAAGAAGAAATTCAAACAAATTTATTGCTTAGTTTAAGA
CTTGCTCAAATCGAAGGTTTGGGAAAAAATTCTATAGAGAATTTATTAAATTATGCAACGCATTCAACTGGTGTTCTTTT
TAATGCTTATGATTTAAGTAGTTTTTCTGAAAAAGATGAAGAATTTACCATTAATTTAAGCAAGCAAGTACACGGAGAAA
CAAAAGTATCTTGCAAAGATAAACTTTTAAGCTTTAGCGGAATTTTCCAAAATAATGAAAGTAGATGTAAAAATGATTCT
AAAATAATTATTATTGAGGCTAATTATACCGATCCATCGGTAATTGCAGATGCTAAAATATATCATAAAGATCAAGATGA
AAAAATGCTTTATAGAAGTTTTAGAGGAAGTGAACCTAAATTAAATTTAGGAATGGATTTTTTACTTGGTATTTTTGAGC
AGATTCCAAATCTTGTTATTTATAGTTCTTCTCAACAAATTTTAACCGACAAAGAATTACCAATCATACCAATAAGCATT
GAAAGTATTGGTGATATAATAGGGCAAAATGTTGATAAAGATGAAGTATTAAAAATATTAAAAAAATTAGGTTTTGAACT
CATACTTTCAGGTGAAGGGCTTATAAATGTTAAAGCACCTTTACATCGTCCTGATATTAAAAATTTATCAGATATTTGTG
AGGAAGTTGTAAGAATTATAGGTATTTATAATATTGCTTCCAAGGGTTTAGAATTTGTAGAAAAAAATCGTTTAAATTCT
GTATATAAAAATTATATTGAGTTTTTGAATTTAAGGAAAAGAGCAGTAGCTAGTGGTTATTTTGAAAGCTTACATTATGT
TTTGGATAATGAAGAAGAACTTAAAAGATTGGGTTTTGACTCTGTTAAATTAAAACTAATCAATCCAATTACAGCAGAAC
TTAATACTTTAAGAACAACCCTTTTAAATCATTTATTAAATGCAGCAAGTTTAAATGCAAAAAATTCTAAAAAAATTATT
AAAATTTTTGAACTAGGGGCTGTTTTCAATGTCAATAATCAAGAATTAAATCGTATTGCATTTATCCATTCCGGCTTAAA
AGAAGAAGCTAAAATTTCTAACAAGGCAAAACCTGAATCGGTCCAATTTTATGATTTTCTTTTAGACATTAAAAATATTA
TAGGTGATTTTAAACTAAAATCATCAAAATATAATATTTTAAGCCCTTATGAACAAGCAGATATTTATCTATCGGATATT
AAAGTAGGTTTTATAGGCCGTTTGCATTTAAAAATAGAAAATGATAGAGATTTACCAAAAACATATATTTGCGAACTTGA
TTTAGATTTAATCAAGCAAGATTTTAAAATAGCTAAGCCTTACTCCAAATTCCCAGCTATCACTAGAGATCTTAGTGTGT
TAATACCTAAAGGTTTTGAATACAATCAGATTAAAAATTGCATCGAAGAATTGAATTTAGAAATGCTTGAAAATTTTCGT
TTAGTTGATATTTATAGTGATGAAAACCTTAAAGAATTTTATAGCATTACTATAAGTTTTTCTTTTAGGGATATAAATAA
AACTCTAGAAGATAATCAAGTAAATGAATGCATGGATAAAATTTTAAATACTCTTAAAAATTTGGGGCTTGATTTAAGAT
GA

Upstream 100 bases:

>100_bases
CTTTTGGATTGGGAGTAGAACGTTTTGCAATGCTTTTGCATCAAATTCCAGATTTAAGATCTTTATTTGAAGGTGATTTG
AGATTATTGGAGCAGTTTAG

Downstream 100 bases:

>100_bases
AAATTTATAAATTGCAAACCCCTGTAAATACTATACTTGAAAATATAGCAGCAGATAAAAGCATATCTCATCGTTTTGCC
ATATTTTCGCTTTTAACACA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKSPDKVVVGYVKEKIKHENSDKLSICQVDIGSETLQIVCGA
ANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCSSLELGFEKINEGIMLLDESIGKLELGRALNTYEIFNDELI
EVELTPNRGDCLSIYGVARDLATALNLNLKEAKPFKESENVLGIGRILRLAAEKELNGLYNYRAIELKEEIQTNLLLSLR
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFTINLSKQVHGETKVSCKDKLLSFSGIFQNNESRCKNDS
KIIIIEANYTDPSVIADAKIYHKDQDEKMLYRSFRGSEPKLNLGMDFLLGIFEQIPNLVIYSSSQQILTDKELPIIPISI
ESIGDIIGQNVDKDEVLKILKKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIYNIASKGLEFVEKNRLNS
VYKNYIEFLNLRKRAVASGYFESLHYVLDNEEELKRLGFDSVKLKLINPITAELNTLRTTLLNHLLNAASLNAKNSKKII
KIFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPESVQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDI
KVGFIGRLHLKIENDRDLPKTYICELDLDLIKQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEMLENFR
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR

Sequences:

>Translated_773_residues
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKSPDKVVVGYVKEKIKHENSDKLSICQVDIGSETLQIVCGA
ANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCSSLELGFEKINEGIMLLDESIGKLELGRALNTYEIFNDELI
EVELTPNRGDCLSIYGVARDLATALNLNLKEAKPFKESENVLGIGRILRLAAEKELNGLYNYRAIELKEEIQTNLLLSLR
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFTINLSKQVHGETKVSCKDKLLSFSGIFQNNESRCKNDS
KIIIIEANYTDPSVIADAKIYHKDQDEKMLYRSFRGSEPKLNLGMDFLLGIFEQIPNLVIYSSSQQILTDKELPIIPISI
ESIGDIIGQNVDKDEVLKILKKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIYNIASKGLEFVEKNRLNS
VYKNYIEFLNLRKRAVASGYFESLHYVLDNEEELKRLGFDSVKLKLINPITAELNTLRTTLLNHLLNAASLNAKNSKKII
KIFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPESVQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDI
KVGFIGRLHLKIENDRDLPKTYICELDLDLIKQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEMLENFR
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR
>Mature_773_residues
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKSPDKVVVGYVKEKIKHENSDKLSICQVDIGSETLQIVCGA
ANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCSSLELGFEKINEGIMLLDESIGKLELGRALNTYEIFNDELI
EVELTPNRGDCLSIYGVARDLATALNLNLKEAKPFKESENVLGIGRILRLAAEKELNGLYNYRAIELKEEIQTNLLLSLR
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFTINLSKQVHGETKVSCKDKLLSFSGIFQNNESRCKNDS
KIIIIEANYTDPSVIADAKIYHKDQDEKMLYRSFRGSEPKLNLGMDFLLGIFEQIPNLVIYSSSQQILTDKELPIIPISI
ESIGDIIGQNVDKDEVLKILKKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIYNIASKGLEFVEKNRLNS
VYKNYIEFLNLRKRAVASGYFESLHYVLDNEEELKRLGFDSVKLKLINPITAELNTLRTTLLNHLLNAASLNAKNSKKII
KIFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPESVQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDI
KVGFIGRLHLKIENDRDLPKTYICELDLDLIKQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEMLENFR
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=805, Percent_Identity=27.5776397515528, Blast_Score=223, Evalue=3e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87338; Mature: 87338

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKSPDKVVVGYVKEKIKHENSD
CEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCC
KLSICQVDIGSETLQIVCGAANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCS
CEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
SLELGFEKINEGIMLLDESIGKLELGRALNTYEIFNDELIEVELTPNRGDCLSIYGVARD
HHHHHHHHHCCCEEEEECCCCCCHHCCCCCHHEECCCCEEEEEECCCCCCEEEHHHHHHH
LATALNLNLKEAKPFKESENVLGIGRILRLAAEKELNGLYNYRAIELKEEIQTNLLLSLR
HHHHHCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHH
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFTINLSKQVHGETKVSCKD
HHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEECHHHCCCCCCCHHH
KLLSFSGIFQNNESRCKNDSKIIIIEANYTDPSVIADAKIYHKDQDEKMLYRSFRGSEPK
HHHHHHHHCCCCHHHCCCCCEEEEEEECCCCCCCEECCHHEECCCHHHHHHHHHCCCCCC
LNLGMDFLLGIFEQIPNLVIYSSSQQILTDKELPIIPISIESIGDIIGQNVDKDEVLKIL
CCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCEEEEEHHHHHHHHCCCCCHHHHHHHH
KKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIYNIASKGLEFVEKNRLNS
HHCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYKNYIEFLNLRKRAVASGYFESLHYVLDNEEELKRLGFDSVKLKLINPITAELNTLRTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
LLNHLLNAASLNAKNSKKIIKIFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPES
HHHHHHHHHHCCCCCCCCEEEEEEECEEEECCHHHHHHHHHHHHCCHHHHHHCCCCCCCC
VQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDIKVGFIGRLHLKIENDRDLPK
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHCCEEEEEEEEEEEEEEEEEECCCCCCCC
TYICELDLDLIKQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEMLENFR
EEEEECCHHHHHHHHHHCCCHHHCCHHHCCHHEEECCCCCHHHHHHHHHHCCHHHHHCEE
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR
EEEEECCCCCCEEEEEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIITKSWLNDWLELEEISSDKIAKTLNSIGIEVDRVGALKSPDKVVVGYVKEKIKHENSD
CEEEHHHHHHHHHHHHCCHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCC
KLSICQVDIGSETLQIVCGAANVDAGQFVAVATKGAIMPNGMEIKEAKLRGVDSCGMLCS
CEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
SLELGFEKINEGIMLLDESIGKLELGRALNTYEIFNDELIEVELTPNRGDCLSIYGVARD
HHHHHHHHHCCCEEEEECCCCCCHHCCCCCHHEECCCCEEEEEECCCCCCEEEHHHHHHH
LATALNLNLKEAKPFKESENVLGIGRILRLAAEKELNGLYNYRAIELKEEIQTNLLLSLR
HHHHHCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHH
LAQIEGLGKNSIENLLNYATHSTGVLFNAYDLSSFSEKDEEFTINLSKQVHGETKVSCKD
HHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEECHHHCCCCCCCHHH
KLLSFSGIFQNNESRCKNDSKIIIIEANYTDPSVIADAKIYHKDQDEKMLYRSFRGSEPK
HHHHHHHHCCCCHHHCCCCCEEEEEEECCCCCCCEECCHHEECCCHHHHHHHHHCCCCCC
LNLGMDFLLGIFEQIPNLVIYSSSQQILTDKELPIIPISIESIGDIIGQNVDKDEVLKIL
CCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCEEEEEHHHHHHHHCCCCCHHHHHHHH
KKLGFELILSGEGLINVKAPLHRPDIKNLSDICEEVVRIIGIYNIASKGLEFVEKNRLNS
HHCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYKNYIEFLNLRKRAVASGYFESLHYVLDNEEELKRLGFDSVKLKLINPITAELNTLRTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
LLNHLLNAASLNAKNSKKIIKIFELGAVFNVNNQELNRIAFIHSGLKEEAKISNKAKPES
HHHHHHHHHHCCCCCCCCEEEEEEECEEEECCHHHHHHHHHHHHCCHHHHHHCCCCCCCC
VQFYDFLLDIKNIIGDFKLKSSKYNILSPYEQADIYLSDIKVGFIGRLHLKIENDRDLPK
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHCCEEEEEEEEEEEEEEEEEECCCCCCCC
TYICELDLDLIKQDFKIAKPYSKFPAITRDLSVLIPKGFEYNQIKNCIEELNLEMLENFR
EEEEECCHHHHHHHHHHCCCHHHCCHHHCCHHEEECCCCCHHHHHHHHHHCCHHHHHCEE
LVDIYSDENLKEFYSITISFSFRDINKTLEDNQVNECMDKILNTLKNLGLDLR
EEEEECCCCCCEEEEEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]