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The map label for this gene is pyrF [H]

Identifier: 153951680

GI number: 153951680

Start: 1394415

End: 1395095

Strand: Direct

Name: pyrF [H]

Synonym: JJD26997_1577

Alternate gene names: 153951680

Gene position: 1394415-1395095 (Clockwise)

Preceding gene: 153952373

Following gene: 153951286

Centisome position: 75.57

GC content: 29.52

Gene sequence:

>681_bases
ATGAAGCTTTGTGTCGCACTTGATCTTTCTACAAAAGAAGAATGCTTGCAACTTACTAAAGAATTAAAAAATTTAGACAT
ATGGTTAAAAGTAGGTTTAAGAGCATATTTAAGAGATGGCTTTAAATTCATAGAAGAACTTAAAAAAGTTGATGATTTTA
AAATATTTCTTGATCTTAAAATTCATGATATTCCCAACACTATGGCAGATGCTTGCGAAGAAATATCAAAACTTGGTATT
GATATGATCAATATCCACGCAAGTGCTGGAAAAATAGCCATGCAAGAAGTTATGACACGGCTTAGCAAATTTTCAAAAAG
ACCTTTAGTTTTAGCAGTTTCTGCTCTTACAAGTTTTGATGAAGAAAATTTCTTTAGTATTTATAGACAAAAAATCGAAG
AAGCTGTAATTAATTTTTCAAAAATATCCTATGAAAATGGACTTGATGGTATGGTATGCTCTGTTTTTGAAAGTAAAATC
ATCAAAGAACACACTCAAAAAAACTTTCTAACTCTCACTCCAGGAATTCGTCCTTTTGGAGAAAAAAATGATGATCAAAA
ACGTGTCGCTAATTTAACAATGGCTAGAGAAAATTTAAGCGATTTTATAGTAGTTGGACGTCCTATTTATAAAGATAATA
ACCCTAGAAAAATTTGTGAAAAAATTCTACAAGAAATTTAA

Upstream 100 bases:

>100_bases
TTGTCATCAATGAAGCTATAGAACTTGCTAAAGAACTTGCTAATGACAATTCTCCAAAATTTATGAATGGAGTTTTAGAT
GCCTTGATAAAGGCTAAAAA

Downstream 100 bases:

>100_bases
TAATCATTATCCTTGTTATATTTTAATAATAAAAATTTTTAATAAGGAAAAATTATGATTATAACCCCTGAGAATTTATA
TAAAAAAAGAAGAGATTTTT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKLCVALDLSTKEECLQLTKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLKIHDIPNTMADACEEISKLGI
DMINIHASAGKIAMQEVMTRLSKFSKRPLVLAVSALTSFDEENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKI
IKEHTQKNFLTLTPGIRPFGEKNDDQKRVANLTMARENLSDFIVVGRPIYKDNNPRKICEKILQEI

Sequences:

>Translated_226_residues
MKLCVALDLSTKEECLQLTKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLKIHDIPNTMADACEEISKLGI
DMINIHASAGKIAMQEVMTRLSKFSKRPLVLAVSALTSFDEENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKI
IKEHTQKNFLTLTPGIRPFGEKNDDQKRVANLTMARENLSDFIVVGRPIYKDNNPRKICEKILQEI
>Mature_226_residues
MKLCVALDLSTKEECLQLTKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLKIHDIPNTMADACEEISKLGI
DMINIHASAGKIAMQEVMTRLSKFSKRPLVLAVSALTSFDEENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKI
IKEHTQKNFLTLTPGIRPFGEKNDDQKRVANLTMARENLSDFIVVGRPIYKDNNPRKICEKILQEI

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=222, Percent_Identity=34.2342342342342, Blast_Score=126, Evalue=9e-31,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25958; Mature: 25958

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLCVALDLSTKEECLQLTKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLK
CCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IHDIPNTMADACEEISKLGIDMINIHASAGKIAMQEVMTRLSKFSKRPLVLAVSALTSFD
EECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHCC
EENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKIIKEHTQKNFLTLTPGIRPFG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCC
EKNDDQKRVANLTMARENLSDFIVVGRPIYKDNNPRKICEKILQEI
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MKLCVALDLSTKEECLQLTKELKNLDIWLKVGLRAYLRDGFKFIEELKKVDDFKIFLDLK
CCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IHDIPNTMADACEEISKLGIDMINIHASAGKIAMQEVMTRLSKFSKRPLVLAVSALTSFD
EECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHCC
EENFFSIYRQKIEEAVINFSKISYENGLDGMVCSVFESKIIKEHTQKNFLTLTPGIRPFG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCC
EKNDDQKRVANLTMARENLSDFIVVGRPIYKDNNPRKICEKILQEI
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]