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The map label for this gene is gltD [H]

Identifier: 153951673

GI number: 153951673

Start: 14822

End: 16267

Strand: Direct

Name: gltD [H]

Synonym: JJD26997_0009

Alternate gene names: 153951673

Gene position: 14822-16267 (Clockwise)

Preceding gene: 153951216

Following gene: 153952083

Centisome position: 0.8

GC content: 34.92

Gene sequence:

>1446_bases
ATGGGAAATACAAGAGGTTTTTTGGACTTTAAAAGAATGGATTTTAAAAAAATTGCTCCTAAAGAAAGGATTTTAAATTA
CAAAGAATTTATAACGCTTTTAGACAAGAAAGAACAAGAAATTCAAGGCGGGCGTTGTATGGATTGTGGTGTGGCCTTTT
GTCATACGGGTGTGATGAGCGAAGGAAAAGATGTGGGCTGTCCTTTAAACAATCTCATTCCTGAATGGAACGATCTTATT
TATCGTTCTTTATGGGAAGAAGCTTATGAAAGACTTGATCTTACCAATCCTTTTCCTGAATTTACCGGTCGTGTTTGCCC
TGCTCCTTGTGAGGATTCTTGTGTGTGTGCGATAAATGGCACAAGTGTGAGTATCAAAAATAATGAATTATCCATTATAG
AAAATGCTTTTAAAGAAAATTTAGTAAGAGTAAATAAACCTAAGCAATACAATGGCAAAAAAATCGCTATCATAGGAAGT
GGGCCTGCTGGGCTTGCTTGTGCTAACACTTTAAATTCGCTAGGTTATAAAGTAAGTGTTTTTGAAAGAAGCGATAAAAT
AGGCGGACTTTTGATGTATGGTATCCCTGATATGAAGCTTGATAAAAGTATAGTTGATAGACGGGTGGATTTGCTTAAAA
AAAGTGGGATTGAATTTAAAGTGAATGAAAATATAGACAGCAAAGATAAGGTTTCAAAACTTCTAAAAGAATTTGATGCT
TTGGTGCTTTGCACAGGAGCAAGTAAGCCTATAGATCTTGATATAGAAGGTAGAAAGCTAAAAGGGGTTGAATTTGCTCT
TGATTTTTTAACGCAAAATACCAAAACTTTGTTAAAAACAGGCAAGGGTGCAGATGCAGCCAAAGGTAAAAATGTCTTGG
TTATAGGAAGCGGGGATACAAGTGTGGATTGTATTGCTGTGGCGACAAGACAAGGGGCTAAATCTATAGTGCGTTTTGAA
AGAAGTCCAAAAAAACCTTTGCAAAGAAGCCAAAACAATCCTTGGCCTTTAAAAGCTGACATTTTTACTACTGATTATGG
TTTAGAAGAAGCCATTGCTGTATATGGCAAAGATCCTAGAGAATATCAAAAAATGACAAAGAAATTTTTAGGGAAAACTT
ATGTAGAAGGCGTTGAAGCAAATGATTTAAAACGCGAGTTTAAAGAAGGCAAGGCTATAAATGTTGAAATTCCAAATTCT
AAAAAAACTTATAAAGTGGATTTAGTACTTTTGGCTATGGGTTTTAGCGGGTGCGAAGAAGCTATCGCTAAAAATTTTGG
AGTGAAATTAGATGAGAAAAACAACATAAGCACAGAAAATTTCCAAACCACTCATAAAAAAATCTTTGCTTGCGGAGATG
CTAGAAAAGGACAATCTTTAGTTGTTTGGGCTATAAAAGATGGTATAGAATGTGCTTTGAGTTTGCATCAAAATTTAGCA
AAATAA

Upstream 100 bases:

>100_bases
GATTGGCTAAAGGAAGATATTGAAAAGAGAAATGAAGAGATTTATAATAGATTAGTAGACTTTTTTAAGCAAAATATTGA
TTAGTTTTAAGGAGTAAAAA

Downstream 100 bases:

>100_bases
TCATAAAAGTTTAAAACTTTTTCGGTGGAGTTTGCAAATTCCAAATTTAGCTATAACTTCTTTGTGGGCTTTTGTGCCAT
AGGCTTTGTTTTTTTCAAAT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MGNTRGFLDFKRMDFKKIAPKERILNYKEFITLLDKKEQEIQGGRCMDCGVAFCHTGVMSEGKDVGCPLNNLIPEWNDLI
YRSLWEEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAINGTSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAIIGS
GPAGLACANTLNSLGYKVSVFERSDKIGGLLMYGIPDMKLDKSIVDRRVDLLKKSGIEFKVNENIDSKDKVSKLLKEFDA
LVLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADAAKGKNVLVIGSGDTSVDCIAVATRQGAKSIVRFE
RSPKKPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPREYQKMTKKFLGKTYVEGVEANDLKREFKEGKAINVEIPNS
KKTYKVDLVLLAMGFSGCEEAIAKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLA
K

Sequences:

>Translated_481_residues
MGNTRGFLDFKRMDFKKIAPKERILNYKEFITLLDKKEQEIQGGRCMDCGVAFCHTGVMSEGKDVGCPLNNLIPEWNDLI
YRSLWEEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAINGTSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAIIGS
GPAGLACANTLNSLGYKVSVFERSDKIGGLLMYGIPDMKLDKSIVDRRVDLLKKSGIEFKVNENIDSKDKVSKLLKEFDA
LVLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADAAKGKNVLVIGSGDTSVDCIAVATRQGAKSIVRFE
RSPKKPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPREYQKMTKKFLGKTYVEGVEANDLKREFKEGKAINVEIPNS
KKTYKVDLVLLAMGFSGCEEAIAKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLA
K
>Mature_480_residues
GNTRGFLDFKRMDFKKIAPKERILNYKEFITLLDKKEQEIQGGRCMDCGVAFCHTGVMSEGKDVGCPLNNLIPEWNDLIY
RSLWEEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAINGTSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAIIGSG
PAGLACANTLNSLGYKVSVFERSDKIGGLLMYGIPDMKLDKSIVDRRVDLLKKSGIEFKVNENIDSKDKVSKLLKEFDAL
VLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADAAKGKNVLVIGSGDTSVDCIAVATRQGAKSIVRFER
SPKKPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPREYQKMTKKFLGKTYVEGVEANDLKREFKEGKAINVEIPNSK
KTYKVDLVLLAMGFSGCEEAIAKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLAK

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=293, Percent_Identity=25.2559726962457, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=32.3651452282158, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1788811, Length=479, Percent_Identity=29.8538622129436, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082180, Length=468, Percent_Identity=30.1282051282051, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1788468, Length=458, Percent_Identity=26.4192139737991, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789243, Length=427, Percent_Identity=25.7611241217799, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17570289, Length=488, Percent_Identity=42.2131147540984, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71984108, Length=486, Percent_Identity=23.6625514403292, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543860, Length=183, Percent_Identity=28.9617486338798, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=484, Percent_Identity=43.3884297520661, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI28574881, Length=474, Percent_Identity=45.9915611814346, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24665539, Length=474, Percent_Identity=45.9915611814346, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24665547, Length=481, Percent_Identity=45.5301455301455, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24665543, Length=481, Percent_Identity=45.5301455301455, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24640763, Length=307, Percent_Identity=28.0130293159609, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18858217, Length=307, Percent_Identity=28.0130293159609, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53386; Mature: 53255

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNTRGFLDFKRMDFKKIAPKERILNYKEFITLLDKKEQEIQGGRCMDCGVAFCHTGVMS
CCCCCCCCCHHHCCHHHCCCHHHHCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCC
EGKDVGCPLNNLIPEWNDLIYRSLWEEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAING
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEEC
TSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAIIGSGPAGLACANTLNSLGYKVSV
CEEEEECCCHHHHHHHHHHCCEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEE
FERSDKIGGLLMYGIPDMKLDKSIVDRRVDLLKKSGIEFKVNENIDSKDKVSKLLKEFDA
EECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCE
LVLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADAAKGKNVLVIGSGDT
EEEEECCCCCEEEEECCCEECCHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEECCCC
SVDCIAVATRQGAKSIVRFERSPKKPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPR
CEEEEEEEHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHCCCCHH
EYQKMTKKFLGKTYVEGVEANDLKREFKEGKAINVEIPNSKKTYKVDLVLLAMGFSGCEE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEECCCCHHHH
AIAKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLA
HHHHHCCCEECCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHC
K
C
>Mature Secondary Structure 
GNTRGFLDFKRMDFKKIAPKERILNYKEFITLLDKKEQEIQGGRCMDCGVAFCHTGVMS
CCCCCCCCHHHCCHHHCCCHHHHCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHCCCC
EGKDVGCPLNNLIPEWNDLIYRSLWEEAYERLDLTNPFPEFTGRVCPAPCEDSCVCAING
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEEC
TSVSIKNNELSIIENAFKENLVRVNKPKQYNGKKIAIIGSGPAGLACANTLNSLGYKVSV
CEEEEECCCHHHHHHHHHHCCEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEE
FERSDKIGGLLMYGIPDMKLDKSIVDRRVDLLKKSGIEFKVNENIDSKDKVSKLLKEFDA
EECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCE
LVLCTGASKPIDLDIEGRKLKGVEFALDFLTQNTKTLLKTGKGADAAKGKNVLVIGSGDT
EEEEECCCCCEEEEECCCEECCHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEECCCC
SVDCIAVATRQGAKSIVRFERSPKKPLQRSQNNPWPLKADIFTTDYGLEEAIAVYGKDPR
CEEEEEEEHHHHHHHHHHHHCCCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHCCCCHH
EYQKMTKKFLGKTYVEGVEANDLKREFKEGKAINVEIPNSKKTYKVDLVLLAMGFSGCEE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEECCCCHHHH
AIAKNFGVKLDEKNNISTENFQTTHKKIFACGDARKGQSLVVWAIKDGIECALSLHQNLA
HHHHHCCCEECCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]