Click here to switch to the map view.

The map label for this gene is menD

Identifier: 153948006

GI number: 153948006

Start: 1724127

End: 1725830

Strand: Direct

Name: menD

Synonym: YpsIP31758_1482

Alternate gene names: 153948006

Gene position: 1724127-1725830 (Clockwise)

Preceding gene: 153948373

Following gene: 153947677

Centisome position: 36.5

GC content: 56.63

Gene sequence:

>1704_bases
ATGTCGACAAGTGTTTTTAACCGTCGTTGGGCGGCACTGCTACTGGAGGCTTTGACCCGCCACGGTGTGCGTCATATCTG
TATTGCCCCAGGGTCTCGCTCTACACCGTTAACGTTAGCGGCTGCCGCTAATCCGTCGTTGGTTTGCCATACCCATTTTG
ATGAGCGCGGTCTGGGCCATCTGGCTCTGGGGCTGGCGAAGGCCTCAACAGAACCGGTGGCGGTCATTGTCACCTCGGGT
ACGGCGGTGGCGAATCTTTATCCTGCGCTAATCGAAGCGGGGTTAACGGGTGAGCGGTTGATATTATTGACCGCTGATCG
CCCACCGGAGTTAATCGATTGCGGTGCCAATCAGGCTATCCGCCAGCAGGGGCTGTTTGCCAGCCATCCAACCCTAAGTG
TTAATCTCCCCAGACCGACGCCCGATATCTCTGCCCGTTGGTTGGTTTCCACACTCGACAGCGCGATGGCGCAATTGCAA
CATGGCGCTTTGCACATTAATTGTCCCTTTGCTGAACCACTGTATGGGGGGGATGAGCAACAGTATGCCGACTGGTCTGC
CTCGCTGGGCGATTGGTGGCAGGATTGTCACCCATGGCTGCGTCAAACCTGTTACCCGCCCTCTCTTTATCAACCACTTG
CACAGCAAGCTGACTGGTTTTTCTGGCGGCAAAAACGTGGAGTCGTGATTGCCGGGCGGATGGGCGCGGAAGAGGGTAGG
CAACTGACCGCATGGGCTGCAATGCTGGGCTGGCCACTGATTGGCGATGTATTGTCACAAACGGGTCAGCCATTGCCTTG
TGCTGACTTATGGCTGGCGCACCCACGGGCGCAAGAAACCCTCGCTCAAGCACAGATTGTGTTGCAATTTGGCAGCAGTC
TGACCAGTAAGCGCCTTTTGCAATGGCAGACTGCATGTCAGCCACAGGAATACTGGCTGGTGGATAGCGCCCCAGGCCGC
CTTGATCCTGCTAACCATCGTGGCCGACGTATTATTTGCCCGGTGGGCGAGTGGTTGAGTCGGCACCCCGCGCAGCGGCG
TACACCTTGGGCCACGGAACTGGCGGCGTATTCGGAAAGTGCGCAAGCACAGGTGATCGAGACGCTGGCCGGCCAATTCA
GTGAGGCCGCCGTGGCACATCAGCTCGCGGAATTATTACCGGATAACGGCCAGCTATTTGTTGGCAACAGTTTGATTATT
CGGTTGATTGATGCGTTGGGACAGCTACCCGCAGGCTATCCGGTTTACAGCAACCGGGGGGCCAGTGGTATCGATGGTTT
GCTGTCAACCGCCGCCGGTGTACAGCGCGCGACGGCGAAACCGACATTAGCTATCGTCGGTGATTTGTCTGCCTTATATG
ACCTTAATGCGCTGGCACTTTTGCGCCAGAGCTCGGCACCGATGGTGCTACTGGTGATCAATAACAATGGGGGGCAGATT
TTCTCTTTATTGCCAACCCCAGAGGCCGAGCGCCAGCGTTTCTACTGTATGCCGCAGGATGTGAACTTCGAACACGCAGC
GGTTATGTTCAGCTTAGGTTATGCCCGGCCTAATAGCTGGCCTCAGCTACGGGAGCACGTGCATCAATGCTGGCTACGGG
GGGGAACCACGCTGATTGAAGTCCAGGTGCCACCAAGTCAGGGGGCAGAAACACTGCAACAGTTAGTACAACAGGTGACA
TTAATACCGCAGGTGGCCCCATGA

Upstream 100 bases:

>100_bases
TTAACCATAAAGCGGTCGCTGCGGATTTTTCAACGGTAACGTTTCTCTTTATCTTAATTATCACGGCCGTAGCGTCTGTT
AACTGACTTTTGAGCAAACC

Downstream 100 bases:

>100_bases
CGACACTGGCTTGTCGAAAACTGGCCCCTCATCCGGAATCGCCCCGCCATCAACACGCCGGGCCTTGGTTGGTTTGGTTA
CATGGTTTGTTGGGCAGCAG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSTSVFNRRWAALLLEALTRHGVRHICIAPGSRSTPLTLAAAANPSLVCHTHFDERGLGHLALGLAKASTEPVAVIVTSG
TAVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAIRQQGLFASHPTLSVNLPRPTPDISARWLVSTLDSAMAQLQ
HGALHINCPFAEPLYGGDEQQYADWSASLGDWWQDCHPWLRQTCYPPSLYQPLAQQADWFFWRQKRGVVIAGRMGAEEGR
QLTAWAAMLGWPLIGDVLSQTGQPLPCADLWLAHPRAQETLAQAQIVLQFGSSLTSKRLLQWQTACQPQEYWLVDSAPGR
LDPANHRGRRIICPVGEWLSRHPAQRRTPWATELAAYSESAQAQVIETLAGQFSEAAVAHQLAELLPDNGQLFVGNSLII
RLIDALGQLPAGYPVYSNRGASGIDGLLSTAAGVQRATAKPTLAIVGDLSALYDLNALALLRQSSAPMVLLVINNNGGQI
FSLLPTPEAERQRFYCMPQDVNFEHAAVMFSLGYARPNSWPQLREHVHQCWLRGGTTLIEVQVPPSQGAETLQQLVQQVT
LIPQVAP

Sequences:

>Translated_567_residues
MSTSVFNRRWAALLLEALTRHGVRHICIAPGSRSTPLTLAAAANPSLVCHTHFDERGLGHLALGLAKASTEPVAVIVTSG
TAVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAIRQQGLFASHPTLSVNLPRPTPDISARWLVSTLDSAMAQLQ
HGALHINCPFAEPLYGGDEQQYADWSASLGDWWQDCHPWLRQTCYPPSLYQPLAQQADWFFWRQKRGVVIAGRMGAEEGR
QLTAWAAMLGWPLIGDVLSQTGQPLPCADLWLAHPRAQETLAQAQIVLQFGSSLTSKRLLQWQTACQPQEYWLVDSAPGR
LDPANHRGRRIICPVGEWLSRHPAQRRTPWATELAAYSESAQAQVIETLAGQFSEAAVAHQLAELLPDNGQLFVGNSLII
RLIDALGQLPAGYPVYSNRGASGIDGLLSTAAGVQRATAKPTLAIVGDLSALYDLNALALLRQSSAPMVLLVINNNGGQI
FSLLPTPEAERQRFYCMPQDVNFEHAAVMFSLGYARPNSWPQLREHVHQCWLRGGTTLIEVQVPPSQGAETLQQLVQQVT
LIPQVAP
>Mature_566_residues
STSVFNRRWAALLLEALTRHGVRHICIAPGSRSTPLTLAAAANPSLVCHTHFDERGLGHLALGLAKASTEPVAVIVTSGT
AVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAIRQQGLFASHPTLSVNLPRPTPDISARWLVSTLDSAMAQLQH
GALHINCPFAEPLYGGDEQQYADWSASLGDWWQDCHPWLRQTCYPPSLYQPLAQQADWFFWRQKRGVVIAGRMGAEEGRQ
LTAWAAMLGWPLIGDVLSQTGQPLPCADLWLAHPRAQETLAQAQIVLQFGSSLTSKRLLQWQTACQPQEYWLVDSAPGRL
DPANHRGRRIICPVGEWLSRHPAQRRTPWATELAAYSESAQAQVIETLAGQFSEAAVAHQLAELLPDNGQLFVGNSLIIR
LIDALGQLPAGYPVYSNRGASGIDGLLSTAAGVQRATAKPTLAIVGDLSALYDLNALALLRQSSAPMVLLVINNNGGQIF
SLLPTPEAERQRFYCMPQDVNFEHAAVMFSLGYARPNSWPQLREHVHQCWLRGGTTLIEVQVPPSQGAETLQQLVQQVTL
IPQVAP

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=560, Percent_Identity=70, Blast_Score=799, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_YERP3 (A7FGT2)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400460.1
- ProteinModelPortal:   A7FGT2
- STRING:   A7FGT2
- GeneID:   5387823
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1482
- NMPDR:   fig|349747.3.peg.1050
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   YPSE349747:YPSIP31758_1482-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 61842; Mature: 61711

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVFNRRWAALLLEALTRHGVRHICIAPGSRSTPLTLAAAANPSLVCHTHFDERGLGH
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCEEEEECCCCCCCCH
LALGLAKASTEPVAVIVTSGTAVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAI
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH
RQQGLFASHPTLSVNLPRPTPDISARWLVSTLDSAMAQLQHGALHINCPFAEPLYGGDEQ
HHCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCH
QYADWSASLGDWWQDCHPWLRQTCYPPSLYQPLAQQADWFFWRQKRGVVIAGRMGAEEGR
HHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHEEEECCCCEEEEECCCCCCCC
QLTAWAAMLGWPLIGDVLSQTGQPLPCADLWLAHPRAQETLAQAQIVLQFGSSLTSKRLL
HHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHH
QWQTACQPQEYWLVDSAPGRLDPANHRGRRIICPVGEWLSRHPAQRRTPWATELAAYSES
HHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCHHHHHHHCCH
AQAQVIETLAGQFSEAAVAHQLAELLPDNGQLFVGNSLIIRLIDALGQLPAGYPVYSNRG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCC
ASGIDGLLSTAAGVQRATAKPTLAIVGDLSALYDLNALALLRQSSAPMVLLVINNNGGQI
CCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
FSLLPTPEAERQRFYCMPQDVNFEHAAVMFSLGYARPNSWPQLREHVHQCWLRGGTTLIE
EEECCCCCHHCCEEEECCCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEE
VQVPPSQGAETLQQLVQQVTLIPQVAP
EECCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STSVFNRRWAALLLEALTRHGVRHICIAPGSRSTPLTLAAAANPSLVCHTHFDERGLGH
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCEEEEECCCCCCCCH
LALGLAKASTEPVAVIVTSGTAVANLYPALIEAGLTGERLILLTADRPPELIDCGANQAI
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH
RQQGLFASHPTLSVNLPRPTPDISARWLVSTLDSAMAQLQHGALHINCPFAEPLYGGDEQ
HHCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCH
QYADWSASLGDWWQDCHPWLRQTCYPPSLYQPLAQQADWFFWRQKRGVVIAGRMGAEEGR
HHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHEEEECCCCEEEEECCCCCCCC
QLTAWAAMLGWPLIGDVLSQTGQPLPCADLWLAHPRAQETLAQAQIVLQFGSSLTSKRLL
HHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHH
QWQTACQPQEYWLVDSAPGRLDPANHRGRRIICPVGEWLSRHPAQRRTPWATELAAYSES
HHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCHHHHHHHCCH
AQAQVIETLAGQFSEAAVAHQLAELLPDNGQLFVGNSLIIRLIDALGQLPAGYPVYSNRG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCC
ASGIDGLLSTAAGVQRATAKPTLAIVGDLSALYDLNALALLRQSSAPMVLLVINNNGGQI
CCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
FSLLPTPEAERQRFYCMPQDVNFEHAAVMFSLGYARPNSWPQLREHVHQCWLRGGTTLIE
EEECCCCCHHCCEEEECCCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEE
VQVPPSQGAETLQQLVQQVTLIPQVAP
EECCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA