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The map label for this gene is menF [H]

Identifier: 153948373

GI number: 153948373

Start: 1722532

End: 1723899

Strand: Direct

Name: menF [H]

Synonym: YpsIP31758_1481

Alternate gene names: 153948373

Gene position: 1722532-1723899 (Clockwise)

Preceding gene: 153947650

Following gene: 153948006

Centisome position: 36.47

GC content: 51.17

Gene sequence:

>1368_bases
GTGAAACAACTTTCTGGTTTGCTGGGTGAATTACGACAGAAATTATGTGCCGGTTTCCCTGAACAGGCAGGTATTCAACA
ACTTATTTTTCCAGCACCCGGCTTGGTGGGCCGCCAATTGTTAGAGTGGTTAACTGCTCAGACTCACTTTCCTCAATTTT
ATTGGCGTCACCGTGACAACCATGAAGAAGCCGCCGTCTGCGGGCAAACCCGCTCATTTGCCGATATGAAAGATGCCGAT
GATTTTATTCAGCAAAACCCTGATGCTAATGGGCTGCGTATTTGGGGCCTCAATGCATTTGAACCGGTGATGGTATTCGG
GAAACTAACAGATAATGGCCTGGTCAGTGGAAAAAACGCCCAAGCCAGCTTTCTTTTTCTACCGCGTCTTGAAATATTAC
GCCGGGGTAAAAAAACCTCGCTCACCCTTAATTTGAGCAGCGAGACCTCCCTACAGAAAGATGCCTTACAGGCCATCACG
TTTATTGACCAATTGATGGCAGCCCGCGCTCTACCTGTGTTGAATGTGCGTATTCAGCACGCGAGTCATACACCGGGATA
TCCGCAATGGCGCAATCTGATACAGCAAGCGCTAAATGATATTGAGCAGCAAAAGCTCGACAAAGTGGTACTGGCACGGA
CCACGACGTTGACGCTGAATAAACCGCTTTCGTGCGCTGCATTTATGGCTGCCAGCCGGCAGGTCAATCACCGTTGTTAT
CACTTTATGTTACGTTTTGATGATCGACAGGCCTTTCTGGGTTCCAGTCCCGAACGGCTCTATCTACGCCAGCAATTACA
TTTAGAAACCGAAGCATTGGCGGGGACTGTCTCTAATCTTGATAGCGACCCACAGGCGGCGGTATTGGCAGATTGGTTGA
TGCATGATGAAAAGAATCAGCGCGAGAATTTATTGGTGGTAGACGATATTTGTCAGCGCTTGCAAGGTGGGGTAACCGCC
GTGGATGTCATGCCGCCAGAAATTATCCGCTTGCGCAAAGTACAGCATTTGCGCCGCCGTATCTGTGCGCAACTCAGCCG
GGCCAGTGATACTGACTGCCTGCAACGCTTGCAGCCGACTGCGGCGGTGGCGGGGTTGCCCCGTGAGGCGGCGCGGCAGT
TTATTGCCAAACATGAGCTATTTTCCCGTGGCTGGTATGCGGGTTCTGCCGGTTATTTGTCACTTAAACGCACTGAATTC
AGTGTGGCCCTGCGTTCAGCGCGAGTCGATGGGCAGCAGATTCACCTGTATGCCGGGGCGGGGATCGTGGCCGGTTCTGA
TGCGGAACAAGAGTGGCAAGAGATCGATAATAAGTCAGCAGGTTTACAAAGCTTGTTGGAGCACGAAGCGCAGCCCGTTA
AAGCCTAA

Upstream 100 bases:

>100_bases
AACATTAGTGTGATAAGTAACAATAACTTTCCAATCCGACTTTTCTTATTATTATCACCCCGTATAATCCCACTCTTTCT
GTTGATTTACCGAGGTGGTC

Downstream 100 bases:

>100_bases
AATGCCGCAATTAACCGCACTTGTTATAGGGACGGATTGTCGCCCCCATGTGGGCGGTGTAGACTGTCAAACCGGAGCCA
CAACTCGGCAAGTATTAGCC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKQLSGLLGELRQKLCAGFPEQAGIQQLIFPAPGLVGRQLLEWLTAQTHFPQFYWRHRDNHEEAAVCGQTRSFADMKDAD
DFIQQNPDANGLRIWGLNAFEPVMVFGKLTDNGLVSGKNAQASFLFLPRLEILRRGKKTSLTLNLSSETSLQKDALQAIT
FIDQLMAARALPVLNVRIQHASHTPGYPQWRNLIQQALNDIEQQKLDKVVLARTTTLTLNKPLSCAAFMAASRQVNHRCY
HFMLRFDDRQAFLGSSPERLYLRQQLHLETEALAGTVSNLDSDPQAAVLADWLMHDEKNQRENLLVVDDICQRLQGGVTA
VDVMPPEIIRLRKVQHLRRRICAQLSRASDTDCLQRLQPTAAVAGLPREAARQFIAKHELFSRGWYAGSAGYLSLKRTEF
SVALRSARVDGQQIHLYAGAGIVAGSDAEQEWQEIDNKSAGLQSLLEHEAQPVKA

Sequences:

>Translated_455_residues
MKQLSGLLGELRQKLCAGFPEQAGIQQLIFPAPGLVGRQLLEWLTAQTHFPQFYWRHRDNHEEAAVCGQTRSFADMKDAD
DFIQQNPDANGLRIWGLNAFEPVMVFGKLTDNGLVSGKNAQASFLFLPRLEILRRGKKTSLTLNLSSETSLQKDALQAIT
FIDQLMAARALPVLNVRIQHASHTPGYPQWRNLIQQALNDIEQQKLDKVVLARTTTLTLNKPLSCAAFMAASRQVNHRCY
HFMLRFDDRQAFLGSSPERLYLRQQLHLETEALAGTVSNLDSDPQAAVLADWLMHDEKNQRENLLVVDDICQRLQGGVTA
VDVMPPEIIRLRKVQHLRRRICAQLSRASDTDCLQRLQPTAAVAGLPREAARQFIAKHELFSRGWYAGSAGYLSLKRTEF
SVALRSARVDGQQIHLYAGAGIVAGSDAEQEWQEIDNKSAGLQSLLEHEAQPVKA
>Mature_455_residues
MKQLSGLLGELRQKLCAGFPEQAGIQQLIFPAPGLVGRQLLEWLTAQTHFPQFYWRHRDNHEEAAVCGQTRSFADMKDAD
DFIQQNPDANGLRIWGLNAFEPVMVFGKLTDNGLVSGKNAQASFLFLPRLEILRRGKKTSLTLNLSSETSLQKDALQAIT
FIDQLMAARALPVLNVRIQHASHTPGYPQWRNLIQQALNDIEQQKLDKVVLARTTTLTLNKPLSCAAFMAASRQVNHRCY
HFMLRFDDRQAFLGSSPERLYLRQQLHLETEALAGTVSNLDSDPQAAVLADWLMHDEKNQRENLLVVDDICQRLQGGVTA
VDVMPPEIIRLRKVQHLRRRICAQLSRASDTDCLQRLQPTAAVAGLPREAARQFIAKHELFSRGWYAGSAGYLSLKRTEF
SVALRSARVDGQQIHLYAGAGIVAGSDAEQEWQEIDNKSAGLQSLLEHEAQPVKA

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=449, Percent_Identity=52.1158129175947, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1786809, Length=239, Percent_Identity=28.4518828451883, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788114, Length=210, Percent_Identity=30.952380952381, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787518, Length=296, Percent_Identity=26.6891891891892, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320935, Length=215, Percent_Identity=32.5581395348837, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 51002; Mature: 51002

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLSGLLGELRQKLCAGFPEQAGIQQLIFPAPGLVGRQLLEWLTAQTHFPQFYWRHRDN
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCC
HEEAAVCGQTRSFADMKDADDFIQQNPDANGLRIWGLNAFEPVMVFGKLTDNGLVSGKNA
CCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHEEEEECCCCCCCCCCC
QASFLFLPRLEILRRGKKTSLTLNLSSETSLQKDALQAITFIDQLMAARALPVLNVRIQH
CEEEEECCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
ASHTPGYPQWRNLIQQALNDIEQQKLDKVVLARTTTLTLNKPLSCAAFMAASRQVNHRCY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHE
HFMLRFDDRQAFLGSSPERLYLRQQLHLETEALAGTVSNLDSDPQAAVLADWLMHDEKNQ
EEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
RENLLVVDDICQRLQGGVTAVDVMPPEIIRLRKVQHLRRRICAQLSRASDTDCLQRLQPT
CCCEEEHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCH
AAVAGLPREAARQFIAKHELFSRGWYAGSAGYLSLKRTEFSVALRSARVDGQQIHLYAGA
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEECC
GIVAGSDAEQEWQEIDNKSAGLQSLLEHEAQPVKA
CEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKQLSGLLGELRQKLCAGFPEQAGIQQLIFPAPGLVGRQLLEWLTAQTHFPQFYWRHRDN
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCC
HEEAAVCGQTRSFADMKDADDFIQQNPDANGLRIWGLNAFEPVMVFGKLTDNGLVSGKNA
CCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHEEEEECCCCCCCCCCC
QASFLFLPRLEILRRGKKTSLTLNLSSETSLQKDALQAITFIDQLMAARALPVLNVRIQH
CEEEEECCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
ASHTPGYPQWRNLIQQALNDIEQQKLDKVVLARTTTLTLNKPLSCAAFMAASRQVNHRCY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHE
HFMLRFDDRQAFLGSSPERLYLRQQLHLETEALAGTVSNLDSDPQAAVLADWLMHDEKNQ
EEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
RENLLVVDDICQRLQGGVTAVDVMPPEIIRLRKVQHLRRRICAQLSRASDTDCLQRLQPT
CCCEEEHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCH
AAVAGLPREAARQFIAKHELFSRGWYAGSAGYLSLKRTEFSVALRSARVDGQQIHLYAGA
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEECC
GIVAGSDAEQEWQEIDNKSAGLQSLLEHEAQPVKA
CEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]